Name: Methylobacterium currus PR1016A
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159850

Type strain:

Species: Methylobacterium currus

Strain Designation: PR1016A

Culture col. no.: KACC 19662, JCM 32670

Strain history: W. Park; Korea Univ., South Korea; PR1016A.

NCBI tax ID(s): 2051553 (species)

Section

Methylobacterium currus PR1016A is an aerobe, methylotroph, mesophilic bacterium that has a pink and red pigmentation and was isolated from biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year.

Gram-negative
motile
pigmented
rod-shaped
aerobe
mesophilic
methylotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylobacteriaceae*
	Genus	*Methylobacterium*
	Species	**Methylobacterium currus**
	Full Scientific Name (LPSN)	Methylobacterium currus Park et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66754]	Gram stain	negative

[Ref.: #66754]	Cell length	2.1-5.1 µm

[Ref.: #66754]	Cell width	0.8-0.9 µm

[Ref.: #66754]	Cell shape	rod-shaped

[Ref.: #66754]	Motility	yes

[Ref.: #66754]	Pigment production	yes
[Ref.: #66754]	Pigmentation color	pink and red
[Ref.: #66754]	Pigment name	Pink and reddish pigments

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66754]

Culture medium

tryptone yeast extract glucose agar

[Ref.: #66754]

Culture medium growth

		Temperature
[Ref.: #66754]	optimum	30 °C
[Ref.: #66754]	growth	18-42 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #66754]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66754]			optimum	7
[Ref.: #66754]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66754]

Oxygen tolerance

aerobe

[Ref.: #66754]

Nutrition type

methylotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66754]		NaCl		growth	0-1.0	%(w/v)
[Ref.: #66754]		NaCl		optimum	0	%(w/v)

[Ref.: #66754]	Observation	Pink and reddish pigments show maximum absorption at 251, 366, 503 and 530 nm on R2A agar media, but at 242, 365 and 489 nm on TYG media.
[Ref.: #66754]	Observation	Growth occurs when 0.1 % methanol is added as the sole carbon and energy source.
[Ref.: #66754]	Observation	Representative genes for methanol dehydrogenase (mxaF) are found and MLSA showed that the mxaF gene in this species is very close to that of M. aquaticum and M. platani (96 % similarity).
[Ref.: #67770]	Observation	quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66754]	adipate ChEBI	+	assimilation
[Ref.: #66754]	cellulose ChEBI	-	hydrolysis
[Ref.: #66754]	D-glucose ChEBI	+	assimilation
[Ref.: #66754]	D-mannitol ChEBI	-	assimilation
[Ref.: #66754]	D-mannose ChEBI	-	assimilation
[Ref.: #66754]	decanoate ChEBI	-	assimilation
[Ref.: #66754]	dna ChEBI	-	hydrolysis
[Ref.: #66754]	L-arabinose ChEBI	+	assimilation
[Ref.: #66754]	malate ChEBI	+	assimilation
[Ref.: #66754]	maltose ChEBI	-	assimilation
[Ref.: #66754]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #66754]	nitrate ChEBI	+	reduction
[Ref.: #66754]	phenylacetate ChEBI	-	assimilation
[Ref.: #66754]	potassium gluconate ChEBI	+	assimilation
[Ref.: #66754]	skimmed milk	-	hydrolysis
[Ref.: #66754]	sodium citrate ChEBI	+	assimilation
[Ref.: #66754]	starch ChEBI	-	hydrolysis
[Ref.: #66754]	tween 60 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66754]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66754]	acid phosphatase	+	3.1.3.2
[Ref.: #66754]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66754]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66754]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66754]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66754]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66754]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66754]	beta-galactosidase	-	3.2.1.23
[Ref.: #66754]	beta-glucosidase	-	3.2.1.21
[Ref.: #66754]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66754]	catalase	+	1.11.1.6
[Ref.: #66754]	cystine arylamidase	+	3.4.11.3
[Ref.: #66754]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66754]	esterase (C 4)	+
[Ref.: #66754]	esterase Lipase (C 8)	+
[Ref.: #66754]	leucine arylamidase	+	3.4.11.1
[Ref.: #66754]	lipase (C 14)	-
[Ref.: #66754]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66754]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66754]	trypsin	+	3.4.21.4
[Ref.: #66754]	urease	+	3.5.1.5
[Ref.: #66754]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66754]	Sample type/isolated from	biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year
[Ref.: #66754]	Country	Republic of Korea
[Ref.: #66754]	Country ISO 3 Code	KOR
[Ref.: #66754]	Continent	Asia
[Ref.: #66754]	Enrichment culture	R2A agar
[Ref.: #66754]	Enrichment culture duration	7 days
[Ref.: #66754]	Enrichment culture temperature	28 ̊C
[Ref.: #66754]	Isolation/enrichment procedure	It was isolated from biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year in Korea. Aluminium heat exchanger fins were harvested, washed with PBS, and the washes were serially diluted and plated onto R2A agar. After incubation at 28°C for 7 days, the strain was isolated.

[Ref.: #67770]	Sample type/isolated from	Biofilms growing on the surface of motor car evaporators
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Biofilm	-
#Engineered	#Built environment	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_00022 16S ribosomal RNA gene, partial sequence	MH158285	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_00456 16S ribosomal RNA gene, partial sequence	MH158286	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_01673 16S ribosomal RNA gene, partial sequence	MH158287	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_02939 16S ribosomal RNA gene, partial sequence	MH158288	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_02991 16S ribosomal RNA gene, partial sequence	MH158289	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_03240 16S ribosomal RNA gene, partial sequence	MH158290	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_03948 16S ribosomal RNA gene, partial sequence	MH158291	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_04412 16S ribosomal RNA gene, partial sequence	MH158292	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_05315 16S ribosomal RNA gene, partial sequence	MH158293	1500	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_06260 16S ribosomal RNA gene, partial sequence	MH158294	1484	ENA	2051553 tax ID
[Ref.: #66754]	Methylobacterium sp. CP-2018 strain MP1016A_06305 16S ribosomal RNA gene, partial sequence	MH158295	1484	ENA	2051553 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylobacterium currus PR1016A	GCA_003058325	chromosome	GenBank	2051553 tax ID	*
[Ref.: #66792]	Methylobacterium currus PR1016A	2841517901	complete	JGI IMG/M	2051553 tax ID	*

[Ref.: #66754]	GC-content	70.2 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	70.2 mol%

Availability in culture collections External links

[Ref.: #66754]

Culture collection no.

KACC 19662, JCM 32670

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing.	Feng GD, Chen W, Zhang XJ, Zhang J, Wang SN, Zhu H	Int J Syst Evol Microbiol	10.1099/ijsem.0.004112	2020	*
Phylogeny	Methylobacterium terricola sp. nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil.	Kim J, Chhetri G, Kim I, Lee B, Jang W, Kim MK, Seo T	Int J Syst Evol Microbiol	10.1099/ijsem.0.004054	2020	*
Phylogeny	Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil.	Kim J, Chhetri G, Kim I, Kim H, Kim MK, Seo T	J Microbiol	10.1007/s12275-019-9007-9	2019	*
Phylogeny	Methylobacterium currus sp. nov., isolated from a car air conditioning system.	Park C, Lee YS, Park SY, Park W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003045	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66754

Chulwoo Park, Yun Suk Lee, So-yoon Park, Woojun Park: Methylobacterium currus sp. nov., isolated from a car air conditioning system. IJSEM 68: 3621 - 3626 2018 ( DOI 10.1099/ijsem.0.003045 , PubMed 30252644 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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