Strain identifier

BacDive ID: 159850

Type strain: Yes

Species: Methylobacterium currus

Strain Designation: PR1016A

Strain history: W. Park; Korea Univ., South Korea; PR1016A.

NCBI tax ID(s): 2051553 (species)

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General

@ref: 66754

BacDive-ID: 159850

keywords: genome sequence, 16S sequence, Bacteria, aerobe, methylotroph, mesophilic, Gram-negative, motile, rod-shaped, pigmented

description: Methylobacterium currus PR1016A is an aerobe, methylotroph, mesophilic bacterium that has a pink and red pigmentation and was isolated from biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year.

NCBI tax id

  • NCBI tax id: 2051553
  • Matching level: species

strain history

  • @ref: 67770
  • history: W. Park; Korea Univ., South Korea; PR1016A.

doi: 10.13145/bacdive159850.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylobacteriaceae
  • genus: Methylobacterium
  • species: Methylobacterium currus
  • full scientific name: Methylobacterium currus Park et al. 2018

@ref: 66754

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Methylobacteriaceae

genus: Methylobacterium

species: Methylobacterium currus

strain designation: PR1016A

type strain: yes

Morphology

cell morphology

  • @ref: 66754
  • gram stain: negative
  • cell length: 2.1-5.1 µm
  • cell width: 0.8-0.9 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 66754
  • production: yes
  • color: pink and red
  • name: Pink and reddish pigments

Culture and growth conditions

culture medium

  • @ref: 66754
  • name: tryptone yeast extract glucose agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66754positiveoptimum30mesophilic
66754positivegrowth18-42
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
66754positiveoptimum7
66754positivegrowth5-9alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66754
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 66754
  • type: methylotroph

halophily

@refsaltgrowthtested relationconcentration
66754NaClpositivegrowth0-1.0 %(w/v)
66754NaClpositiveoptimum0 %(w/v)

observation

@refobservation
66754Pink and reddish pigments show maximum absorption at 251, 366, 503 and 530 nm on R2A agar media, but at 242, 365 and 489 nm on TYG media.
66754Growth occurs when 0.1 % methanol is added as the sole carbon and energy source.
66754Representative genes for methanol dehydrogenase (mxaF) are found and MLSA showed that the mxaF gene in this species is very close to that of M. aquaticum and M. platani (96 % similarity).
67770quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6675416024D-mannose-assimilation
6675416899D-mannitol-assimilation
6675417306maltose-assimilation
6675418401phenylacetate-assimilation
6675427689decanoate-assimilation
6675459640N-acetylglucosamine-assimilation
6675417128adipate+assimilation
6675417634D-glucose+assimilation
6675425115malate+assimilation
6675430849L-arabinose+assimilation
6675432032potassium gluconate+assimilation
6675453258sodium citrate+assimilation
6675416991dna-hydrolysis
6675428017starch-hydrolysis
6675453425tween 60-hydrolysis
6675462968cellulose-hydrolysis
66754skimmed milk-hydrolysis
6675417632nitrate+reduction

metabolite production

  • @ref: 66754
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66754alpha-chymotrypsin-3.4.21.1
66754alpha-fucosidase-3.2.1.51
66754alpha-galactosidase-3.2.1.22
66754alpha-glucosidase-3.2.1.20
66754alpha-mannosidase-3.2.1.24
66754beta-galactosidase-3.2.1.23
66754beta-glucosidase-3.2.1.21
66754beta-glucuronidase-3.2.1.31
66754lipase (C 14)-
66754N-acetyl-beta-glucosaminidase-3.2.1.52
66754valine arylamidase-
66754acid phosphatase+3.1.3.2
66754alkaline phosphatase+3.1.3.1
66754catalase+1.11.1.6
66754cystine arylamidase+3.4.11.3
66754cytochrome oxidase+1.9.3.1
66754esterase (C 4)+
66754esterase Lipase (C 8)+
66754leucine arylamidase+3.4.11.1
66754naphthol-AS-BI-phosphohydrolase+
66754trypsin+3.4.21.4
66754urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66754biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 yearRepublic of KoreaKORAsiaR2A agar7 days28It was isolated from biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year in Korea. Aluminium heat exchanger fins were harvested, washed with PBS, and the washes were serially diluted and plated onto R2A agar. After incubation at 28°C for 7 days, the strain was isolated.
67770Biofilms growing on the surface of motor car evaporatorsRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2
#Environmental#Biofilm
#Engineered#Built environment

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
66754Methylobacterium sp. CP-2018 strain MP1016A_00022 16S ribosomal RNA gene, partial sequenceMH1582851484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_00456 16S ribosomal RNA gene, partial sequenceMH1582861484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_01673 16S ribosomal RNA gene, partial sequenceMH1582871484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_02939 16S ribosomal RNA gene, partial sequenceMH1582881484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_02991 16S ribosomal RNA gene, partial sequenceMH1582891484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_03240 16S ribosomal RNA gene, partial sequenceMH1582901484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_03948 16S ribosomal RNA gene, partial sequenceMH1582911484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_04412 16S ribosomal RNA gene, partial sequenceMH1582921484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_05315 16S ribosomal RNA gene, partial sequenceMH1582931500ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_06260 16S ribosomal RNA gene, partial sequenceMH1582941484ena2051553
66754Methylobacterium sp. CP-2018 strain MP1016A_06305 16S ribosomal RNA gene, partial sequenceMH1582951484ena2051553

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methylobacterium currus PR1016AGCA_003058325chromosomencbi2051553
66792Methylobacterium currus PR1016A2841517901completeimg2051553

GC content

@refGC-contentmethod
6675470.2genome sequence analysis
6777070.2

External links

@ref: 66754

culture collection no.: KACC 19662, JCM 32670

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30252644Methylobacterium currus sp. nov., isolated from a car air conditioning system.Park C, Lee YS, Park SY, Park WInt J Syst Evol Microbiol10.1099/ijsem.0.0030452018*Air Conditioning, *Automobiles, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistryTranscriptome
Phylogeny31463788Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil.Kim J, Chhetri G, Kim I, Kim H, Kim MK, Seo TJ Microbiol10.1007/s12275-019-9007-92019Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Gamma Rays, Genotype, Lipids/chemistry, Methylobacterium/*classification/genetics/*isolation & purification/radiation effects, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Radiation Tolerance, Republic of Korea, Sequence Analysis, DNA, Soil, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistry, Whole Genome SequencingGenetics
Phylogeny32100693Methylobacterium terricola sp. nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil.Kim J, Chhetri G, Kim I, Lee B, Jang W, Kim MK, Seo TInt J Syst Evol Microbiol10.1099/ijsem.0.0040542020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Gamma Rays, Methylobacterium/*classification/isolation & purification/radiation effects, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistryTranscriptome
Phylogeny32207677Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing.Feng GD, Chen W, Zhang XJ, Zhang J, Wang SN, Zhu HInt J Syst Evol Microbiol10.1099/ijsem.0.0041122020Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Methylobacterium/*classification/isolation & purification, *Mining, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Tungsten, Ubiquinone/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66754Chulwoo Park, Yun Suk Lee, So-yoon Park, Woojun ParkMethylobacterium currus sp. nov., isolated from a car air conditioning system10.1099/ijsem.0.003045IJSEM 68: 3621-3626 201830252644
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/