Strain identifier
BacDive ID: 159850
Type strain:
Species: Methylobacterium currus
Strain Designation: PR1016A
Strain history: W. Park; Korea Univ., South Korea; PR1016A.
NCBI tax ID(s): 2051553 (species)
General
@ref: 66754
BacDive-ID: 159850
keywords: genome sequence, 16S sequence, Bacteria, aerobe, methylotroph, mesophilic, Gram-negative, motile, rod-shaped, pigmented
description: Methylobacterium currus PR1016A is an aerobe, methylotroph, mesophilic bacterium that has a pink and red pigmentation and was isolated from biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year.
NCBI tax id
- NCBI tax id: 2051553
- Matching level: species
strain history
- @ref: 67770
- history: W. Park; Korea Univ., South Korea; PR1016A.
doi: 10.13145/bacdive159850.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylobacterium
- species: Methylobacterium currus
- full scientific name: Methylobacterium currus Park et al. 2018
@ref: 66754
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Hyphomicrobiales
family: Methylobacteriaceae
genus: Methylobacterium
species: Methylobacterium currus
strain designation: PR1016A
type strain: yes
Morphology
cell morphology
- @ref: 66754
- gram stain: negative
- cell length: 2.1-5.1 µm
- cell width: 0.8-0.9 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 66754
- production: yes
- color: pink and red
- name: Pink and reddish pigments
Culture and growth conditions
culture medium
- @ref: 66754
- name: tryptone yeast extract glucose agar
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
66754 | positive | optimum | 30 | mesophilic |
66754 | positive | growth | 18-42 | |
67770 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
66754 | positive | optimum | 7 | |
66754 | positive | growth | 5-9 | alkaliphile |
Physiology and metabolism
oxygen tolerance
- @ref: 66754
- oxygen tolerance: aerobe
nutrition type
- @ref: 66754
- type: methylotroph
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
66754 | NaCl | positive | growth | 0-1.0 %(w/v) |
66754 | NaCl | positive | optimum | 0 %(w/v) |
observation
@ref | observation |
---|---|
66754 | Pink and reddish pigments show maximum absorption at 251, 366, 503 and 530 nm on R2A agar media, but at 242, 365 and 489 nm on TYG media. |
66754 | Growth occurs when 0.1 % methanol is added as the sole carbon and energy source. |
66754 | Representative genes for methanol dehydrogenase (mxaF) are found and MLSA showed that the mxaF gene in this species is very close to that of M. aquaticum and M. platani (96 % similarity). |
67770 | quinones: Q-10 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
66754 | 16024 | D-mannose | - | assimilation |
66754 | 16899 | D-mannitol | - | assimilation |
66754 | 17306 | maltose | - | assimilation |
66754 | 18401 | phenylacetate | - | assimilation |
66754 | 27689 | decanoate | - | assimilation |
66754 | 59640 | N-acetylglucosamine | - | assimilation |
66754 | 17128 | adipate | + | assimilation |
66754 | 17634 | D-glucose | + | assimilation |
66754 | 25115 | malate | + | assimilation |
66754 | 30849 | L-arabinose | + | assimilation |
66754 | 32032 | potassium gluconate | + | assimilation |
66754 | 53258 | sodium citrate | + | assimilation |
66754 | 16991 | dna | - | hydrolysis |
66754 | 28017 | starch | - | hydrolysis |
66754 | 53425 | tween 60 | - | hydrolysis |
66754 | 62968 | cellulose | - | hydrolysis |
66754 | skimmed milk | - | hydrolysis | |
66754 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 66754
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
66754 | alpha-chymotrypsin | - | 3.4.21.1 |
66754 | alpha-fucosidase | - | 3.2.1.51 |
66754 | alpha-galactosidase | - | 3.2.1.22 |
66754 | alpha-glucosidase | - | 3.2.1.20 |
66754 | alpha-mannosidase | - | 3.2.1.24 |
66754 | beta-galactosidase | - | 3.2.1.23 |
66754 | beta-glucosidase | - | 3.2.1.21 |
66754 | beta-glucuronidase | - | 3.2.1.31 |
66754 | lipase (C 14) | - | |
66754 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
66754 | valine arylamidase | - | |
66754 | acid phosphatase | + | 3.1.3.2 |
66754 | alkaline phosphatase | + | 3.1.3.1 |
66754 | catalase | + | 1.11.1.6 |
66754 | cystine arylamidase | + | 3.4.11.3 |
66754 | cytochrome oxidase | + | 1.9.3.1 |
66754 | esterase (C 4) | + | |
66754 | esterase Lipase (C 8) | + | |
66754 | leucine arylamidase | + | 3.4.11.1 |
66754 | naphthol-AS-BI-phosphohydrolase | + | |
66754 | trypsin | + | 3.4.21.4 |
66754 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|
66754 | biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year | Republic of Korea | KOR | Asia | R2A agar | 7 days | 28 | It was isolated from biofilms growing on the surface of motor car evaporators disassembled from a sports utility vehicle and four saloon cars that had been used for more than 1 year in Korea. Aluminium heat exchanger fins were harvested, washed with PBS, and the washes were serially diluted and plated onto R2A agar. After incubation at 28°C for 7 days, the strain was isolated. |
67770 | Biofilms growing on the surface of motor car evaporators | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 |
---|---|
#Environmental | #Biofilm |
#Engineered | #Built environment |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
66754 | Methylobacterium sp. CP-2018 strain MP1016A_00022 16S ribosomal RNA gene, partial sequence | MH158285 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_00456 16S ribosomal RNA gene, partial sequence | MH158286 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_01673 16S ribosomal RNA gene, partial sequence | MH158287 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_02939 16S ribosomal RNA gene, partial sequence | MH158288 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_02991 16S ribosomal RNA gene, partial sequence | MH158289 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_03240 16S ribosomal RNA gene, partial sequence | MH158290 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_03948 16S ribosomal RNA gene, partial sequence | MH158291 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_04412 16S ribosomal RNA gene, partial sequence | MH158292 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_05315 16S ribosomal RNA gene, partial sequence | MH158293 | 1500 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_06260 16S ribosomal RNA gene, partial sequence | MH158294 | 1484 | ena | 2051553 |
66754 | Methylobacterium sp. CP-2018 strain MP1016A_06305 16S ribosomal RNA gene, partial sequence | MH158295 | 1484 | ena | 2051553 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methylobacterium currus PR1016A | GCA_003058325 | chromosome | ncbi | 2051553 |
66792 | Methylobacterium currus PR1016A | 2841517901 | complete | img | 2051553 |
GC content
@ref | GC-content | method |
---|---|---|
66754 | 70.2 | genome sequence analysis |
67770 | 70.2 |
External links
@ref: 66754
culture collection no.: KACC 19662, JCM 32670
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 30252644 | Methylobacterium currus sp. nov., isolated from a car air conditioning system. | Park C, Lee YS, Park SY, Park W | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003045 | 2018 | *Air Conditioning, *Automobiles, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 31463788 | Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil. | Kim J, Chhetri G, Kim I, Kim H, Kim MK, Seo T | J Microbiol | 10.1007/s12275-019-9007-9 | 2019 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Gamma Rays, Genotype, Lipids/chemistry, Methylobacterium/*classification/genetics/*isolation & purification/radiation effects, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Radiation Tolerance, Republic of Korea, Sequence Analysis, DNA, Soil, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistry, Whole Genome Sequencing | Genetics |
Phylogeny | 32100693 | Methylobacterium terricola sp. nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil. | Kim J, Chhetri G, Kim I, Lee B, Jang W, Kim MK, Seo T | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004054 | 2020 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Gamma Rays, Methylobacterium/*classification/isolation & purification/radiation effects, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 32207677 | Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing. | Feng GD, Chen W, Zhang XJ, Zhang J, Wang SN, Zhu H | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004112 | 2020 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Methylobacterium/*classification/isolation & purification, *Mining, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Tungsten, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66754 | Chulwoo Park, Yun Suk Lee, So-yoon Park, Woojun Park | Methylobacterium currus sp. nov., isolated from a car air conditioning system | 10.1099/ijsem.0.003045 | IJSEM 68: 3621-3626 2018 | 30252644 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |