Name: Parageobacillus yumthangensis AYN2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159838

Type strain:

Species: Parageobacillus yumthangensis

Strain Designation: AYN2

Culture col. no.: MTCC 12749, KCTC 33950, JCM 32596, MTCC 12769

Strain history: <- Nagendra Thakur, Sikkim Univ.

NCBI tax ID(s): 2037913 (species)

Special Collections

Section

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Parageobacillus yumthangensis AYN2 is an aerobe, spore-forming, thermophilic bacterium that forms circular colonies and has a creamy-white pigmentation.

colony-forming
Gram-positive
pigmented
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Parageobacillus*
	Species	**Parageobacillus yumthangensis**
	Full Scientific Name (LPSN)	Parageobacillus yumthangensis (Najar et al. 2018) Najar et al. 2020

Synonym

Geobacillus yumthangensis

Information on morphological and physiological properties Morphology

[Ref.: #66718]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #66718]	Cell length	2.5-5 µm

[Ref.: #66718]	Cell width	0.4-0.6 µm

[Ref.: #66718]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #66718]	Motility	yes

[Ref.: #66718]	Colony color	creamy-white
[Ref.: #66718]	Colony shape	circular
[Ref.: #66718]	Incubation period	1 day
[Ref.: #66718]	Cultivation medium used	actinomycetes agar

[Ref.: #66718]	Pigment production	yes
[Ref.: #66718]	Pigmentation color	creamy-white

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66718]

Culture medium

actinomycetes agar

[Ref.: #66718]

Culture medium growth

[Ref.: #66718]

Culture medium composition

expand / minimize

[Ref.: #66718]

Culture medium

solid thermus agar

[Ref.: #66718]

Culture medium growth

[Ref.: #66718]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #66718]		optimum	60 °C
[Ref.: #66718]	negative	growth	80 °C
[Ref.: #66718]		growth	40-70 °C
[Ref.: #67770]		growth	60 °C
[Ref.: #67771]		growth	60 °C

	Kind of pH		pH
[Ref.: #66718]		optimum	7.5-8.0
[Ref.: #66718]	no	growth	0-6
[Ref.: #66718]	no	growth	10-12
[Ref.: #66718]		growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66718]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #66718]	Spore description	spores are located at the sub-terminal to terminal positions
[Ref.: #66718]	Type of spore	endospore
[Ref.: #66718]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66718]		NaCl		growth	0-5	%(w/v)
[Ref.: #66718]		NaCl		optimum	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66718]	acetic acid ChEBI	+	assimilation
[Ref.: #66718]	arabinose ChEBI	-	builds acid from
[Ref.: #66718]	casein	-	hydrolysis
[Ref.: #66718]	cellobiose ChEBI	+	builds acid from
[Ref.: #66718]	formate ChEBI	+	assimilation
[Ref.: #66718]	galactose ChEBI	+	builds acid from
[Ref.: #66718]	glucose ChEBI	+	builds acid from
[Ref.: #66718]	lactate ChEBI	+	assimilation
[Ref.: #66718]	lactose ChEBI	+	builds acid from
[Ref.: #66718]	ribose ChEBI	-	builds acid from
[Ref.: #66718]	sorbitol ChEBI	+	builds acid from
[Ref.: #66718]	starch ChEBI	+	hydrolysis
[Ref.: #66718]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66718]		catalase	+	1.11.1.6

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66718]

Fatty acid	Percentage
C16:0 iso	13.9
C17:0 iso	13.7
C15:0 iso	12.8
C16:0	7
C17:0	4.7
C14:0	3.3
a-C17:0	3.2
C18:1	3.2
C18:0	2.6
C18:0 iso	2.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66718]	Sample type/isolated from	the source of the Yumthang hot spring, North Sikkim, India
[Ref.: #66718]	Geographic location (country and/or sea, region)	Yumthang hot spring, North Sikkim
[Ref.: #66718]	Country	India
[Ref.: #66718]	Country ISO 3 Code	IND
[Ref.: #66718]	Continent	Asia
[Ref.: #66718]	Geographic location	27.7929°/88.7086°
[Ref.: #66718]	Enrichment culture	solid thermus agar
[Ref.: #66718]	Enrichment culture composition	8 gl-1 peptone, 4 gl-1 yeast extract, 2 gl-1 NaCl
[Ref.: #66718]	Enrichment culture duration	24 h
[Ref.: #66718]	Enrichment culture temperature	60 ̊C
[Ref.: #66718]	Isolation/enrichment procedure	Water samples were taken from the source of the Yumthang hot spring, North Sikkim, India. Strain was isolated on solid thermus agar containing 8 gl-1 peptone, 4 gl-1 yeast extract and 2 gl-1 1NaCl with an incubation of 24h at 60°C.

[Ref.: #67770]	Sample type/isolated from	Yumthang hot spring
[Ref.: #67770]	Geographic location (country and/or sea, region)	North Sikkim
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From yumthang hot spring
[Ref.: #67771]	Geographic location (country and/or sea, region)	North Sikkim
[Ref.: #67771]	Country	India
[Ref.: #67771]	Country ISO 3 Code	IND
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Thermal spring

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66718]	Parageobacillus yumthangensis strain AYN2 16S ribosomal RNA gene, partial sequence	MG603320	1457	GenBank ENA	2037913 tax ID
[Ref.: #67770]	Parageobacillus yumthangensis strain AYN2 DnaJ (dnaJ) gene, complete cds	MG657000	1143	GenBank ENA	2037913 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Parageobacillus yumthangensis AYN2	GCA_002494375	scaffold	GenBank ENA	2037913 tax ID
[Ref.: #66792]	Parageobacillus yumthangensis AYN2	2791355095	draft	JGI IMG/M	2037913 tax ID	*

[Ref.: #66718]	GC-content	42.11 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	42.11 mol%
[Ref.: #67770]	GC-content	42.11 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Parageobacillus yumthangensis AYN2 NCBI: GCA_002494375
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	yes	84.8
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	77.3
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	variable	82.4
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	89

Availability in culture collections External links

[Ref.: #66718]

Culture collection no.

MTCC 12749, KCTC 33950, JCM 32596, MTCC 12769

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66718

Ishfaq Nabi Najar, Mingma Thundu Sherpa, Sayak Das, Kamalesh Verma, Vikash Kumar Dubey, Nagendra Thakur: Geobacillus yumthangensis sp. nov., a thermophilic bacterium isolated from a north-east Indian hot spring. IJSEM 68: 3430 - 3434 2018 ( DOI 10.1099/ijsem.0.003002 , PubMed 30222099 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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