Name: Streptacidiphilus pinicola MMS16-CNU450
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159753

Type strain:

Species: Streptacidiphilus pinicola

Strain Designation: MMS16-CNU450

Culture col. no.: KCTC 49008, JCM 32300

Strain history: <- Seung Bum Kim, Chungnam National Univ.

NCBI tax ID(s): 2219663 (species)

Section

Streptacidiphilus pinicola MMS16-CNU450 is an aerobe, spore-forming, mesophilic bacterium that was isolated from pine grove soil .

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptacidiphilus*
	Species	**Streptacidiphilus pinicola**
	Full Scientific Name (LPSN)	Streptacidiphilus pinicola Roh et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66683]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %94.303

[Ref.: #66683]	Multicellular complex forming ability	yes
[Ref.: #66683]	Multicellular complex color	yellowish-brown, beige
[Ref.: #66683]	Medium Name (multicellularity)	acidified starch casein agar, ISP2 agar, ISP3 agar, modified Bennett's agar and potato dextrose agar plates
[Ref.: #66683]	Multicellular complex further description	actinobacterium forming yellowish-brown and beige substrate mycelium and abundant yellowish brown, ivory, beige and dark beige aerialmycelium

Information on culture and growth conditions Culture and growth conditions

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Culture medium

acidified starch casein agar

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Culture medium growth

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Culture medium

Czapek-Dox agar

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Culture medium growth

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 4

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Culture medium growth

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Culture medium

ISP 5

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Culture medium growth

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Culture medium

ISP 6

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Culture medium growth

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Culture medium

ISP 7

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Culture medium growth

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Culture medium

modified Bennett's agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

potato dextrose agar

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

		Temperature
[Ref.: #66683]	optimum	30 °C
[Ref.: #66683]	growth	15-33 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #66683]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66683]			optimum	6
[Ref.: #66683]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66683]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #66683]	Spore description	Aerial mycelia differentiate into straight chains of spores (0.6 x 0.9 µm), and the spore surface is smooth.
[Ref.: #66683]	Type of spore	spore
[Ref.: #66683]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.699
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66683]		NaCl		growth	0-3	%
[Ref.: #66683]		NaCl		optimum	0.5	%

[Ref.: #66683]	Observation	Yellowish brown and beige soluble pigments are formed on acidified starch casein agar and ISP2 agar.
[Ref.: #67770]	Observation	quinones: MK-9(H₆), MK-9(H₈), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66683]	adipate ChEBI	-	carbon source
[Ref.: #66683]	arabinose ChEBI	-	carbon source
[Ref.: #66683]	casein	-	degradation
[Ref.: #66683]	cellulose ChEBI	-	degradation
[Ref.: #66683]	chitin ChEBI	+	degradation
[Ref.: #66683]	chitosan ChEBI	-	degradation
[Ref.: #66683]	citric acid ChEBI	-	carbon source
[Ref.: #66683]	decanoate ChEBI	-	carbon source
[Ref.: #66683]	dna ChEBI	+	degradation
[Ref.: #66683]	gluconate ChEBI	+	carbon source
[Ref.: #66683]	glucose ChEBI	+	carbon source
[Ref.: #66683]	hypoxanthine ChEBI	+	degradation
[Ref.: #66683]	L-alanine ChEBI	+	nitrogen source
[Ref.: #66683]	L-arginine ChEBI	-	nitrogen source
[Ref.: #66683]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #66683]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #66683]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #66683]	L-histidine ChEBI	-	nitrogen source
[Ref.: #66683]	L-isoleucine ChEBI	+	nitrogen source
[Ref.: #66683]	L-phenylalanine ChEBI	+	nitrogen source
[Ref.: #66683]	L-serine ChEBI	+	nitrogen source
[Ref.: #66683]	L-threonine ChEBI	+	nitrogen source
[Ref.: #66683]	L-valine ChEBI	+	nitrogen source
[Ref.: #66683]	malate ChEBI	-	carbon source
[Ref.: #66683]	maltose ChEBI	-	carbon source
[Ref.: #66683]	mannitol ChEBI	+	carbon source
[Ref.: #66683]	mannose ChEBI	-	carbon source
[Ref.: #66683]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #66683]	phenylacetate ChEBI	-	carbon source
[Ref.: #66683]	starch ChEBI	+	degradation
[Ref.: #66683]	tributyrin ChEBI	-	degradation
[Ref.: #66683]	tween 20 ChEBI	-	degradation
[Ref.: #66683]	tween 40 ChEBI	+	degradation
[Ref.: #66683]	tween 80 ChEBI	-	degradation
[Ref.: #66683]	tyrosine ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66683]	acid phosphatase	+	3.1.3.2
[Ref.: #66683]	alkaline phosphatase	-	3.1.3.1
[Ref.: #66683]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66683]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66683]	alpha-galactosidase	+	3.2.1.22
[Ref.: #66683]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66683]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66683]	beta-galactosidase	+	3.2.1.23
[Ref.: #66683]	beta-glucosidase	+	3.2.1.21
[Ref.: #66683]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66683]	cystine arylamidase	+	3.4.11.3
[Ref.: #66683]	esterase (C 4)	+
[Ref.: #66683]	esterase Lipase (C 8)	+
[Ref.: #66683]	leucine arylamidase	+	3.4.11.1
[Ref.: #66683]	lipase (C 14)	-
[Ref.: #66683]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66683]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66683]	trypsin	+	3.4.21.4
[Ref.: #66683]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C16:0 iso	40
C15:0 iso	12.7
C15:0 anteiso	10.4
C16:0	9.8
C14:0 iso	7.1
C17:0 anteiso	4.2
C16:1 iso H	4
C17:0 iso	3.2
C16:1 w7c/ C16:1 w6c	2.3
C17:1 iso w9c / C16:0 10-methyl	1.7
C17:0 cyclo	1.2
C17:1 anteiso w9c	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66683]	Sample type/isolated from	pine grove soil (pH 4.54)
[Ref.: #66683]	Geographic location (country and/or sea, region)	Daejeon
[Ref.: #66683]	Country	Republic of Korea
[Ref.: #66683]	Country ISO 3 Code	KOR
[Ref.: #66683]	Continent	Asia
[Ref.: #66683]	Geographic location	36.3702°/127.3510°
[Ref.: #66683]	Enrichment culture	ISP2
[Ref.: #66683]	Enrichment culture composition	adjusted to pH 5.0 and supplemented with antifungal antibiotics cycloheximide and nystatin (each at 50 µg ml-1 final concentration)

[Ref.: #67770]	Sample type/isolated from	Acidic pine grove soil from Daejeon
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Chungnam National University
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Grove (Orchard)
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MG189375 (>99% sequence identity) for Streptacidiphilus from Microbeatlas

Aquatic Samples	2
Soil Samples	10
Animal Samples
Plant Samples
Total Samples	12

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66683]	Streptacidiphilus pinicola strain MMS16-CNU450 16S ribosomal RNA gene, partial sequence	MG189375	1450	ENA	2219663 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Streptacidiphilus pinicola MMS16-CNU450	GCA_003258295	scaffold	GenBank	2219663 tax ID
[Ref.: #66792]	Streptacidiphilus pinicola MMS16-CNU450	2856758937	draft	JGI IMG/M	2219663 tax ID	*

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GC-content

68.3-68.9 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptacidiphilus pinicola MMS16-CNU450 NCBI: GCA_003258295.1
[Ref.: #69481]	spore-forming	yes	100	no

Availability in culture collections External links

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Culture collection no.

KCTC 49008, JCM 32300

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptacidiphilus pinicola sp. nov., isolated from pine grove soil.	Roh SG, Kim MK, Park S, Yun BR, Park J, Kim MJ, Kim YS, Kim SB	Int J Syst Evol Microbiol	10.1099/ijsem.0.002957	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66683

Su Gwon Roh, Min-Kyeong Kim, Sewook Park, Bo-Ram Yun, Jisun Park, Min Ji Kim, Yeong Seok Kim, Seung Bum Kim: Streptacidiphilus pinicola sp. nov., isolated from pine grove soil. IJSEM 68: 3149 - 3155 2018 ( DOI 10.1099/ijsem.0.002957 , PubMed 30102143 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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