Name: Rhodoluna limnophila 27D-LEPI
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159679

Type strain:

Species: Rhodoluna limnophila

Strain Designation: 27D-LEPI

Culture col. no.: DSM 107804, JCM 32926

Strain history: M. W. Hahn; Res. Dept. for Limnol., Univ. of Innsbruck, Austria; 27D-LEPI.

NCBI tax ID(s): 232537 (species)

SI-ID 398667

DSM 107804

Special Collections

Other strains from Rhodoluna limnophila

Section

Rhodoluna limnophila 27D-LEPI is an aerobe, chemoorganotroph, Gram-positive bacterium that forms circular colonies and was isolated from freshwater.

colony-forming
Gram-positive
rod-shaped
aerobe
chemoorganotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Rhodoluna*
	Species	**Rhodoluna limnophila**
	Full Scientific Name (LPSN)	Rhodoluna limnophila Pitt et al. 2019

Synonym

"Candidatus Rhodoluna limnophila"

Information on morphological and physiological properties Morphology

[Ref.: #68248]	Gram stain	positive

[Ref.: #68248]	Cell length	0.5 µm

[Ref.: #68248]	Cell width	0.3 µm

[Ref.: #68248]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	no Confidence in %93.5

[Ref.: #68248]	Colony color	light red
[Ref.: #68248]	Colony shape	circular
[Ref.: #68248]	Cultivation medium used	NSY agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66530]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #66530]

Culture medium growth

[Ref.: #66530]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66530]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #66530]	growth	22 °C
[Ref.: #67770]	growth	25 °C
[Ref.: #68248]	growth	6-34 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68248]

Oxygen tolerance

aerobe

[Ref.: #68248]

Nutrition type

chemoorganotroph

[Ref.: #69480]

Ability of spore formation

no Confidence in %90.053

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68248]		NaCl		growth	0-0.4	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-11

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68248]	(-)-quinic acid ChEBI	-	assimilation
[Ref.: #68248]	2-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #68248]	4-hydroxyphenylacetic acid ChEBI	-	assimilation
[Ref.: #68248]	acetoacetate ChEBI	+	assimilation
[Ref.: #68248]	alpha-D-glucose ChEBI	+	assimilation
[Ref.: #68248]	alpha-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #68248]	beta-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #68248]	bromosuccinate ChEBI	-	assimilation
[Ref.: #68248]	cellobiose ChEBI	-	assimilation
[Ref.: #68248]	citrate ChEBI	-	assimilation
[Ref.: #68248]	D-fructose ChEBI	+	assimilation
[Ref.: #68248]	D-fructose 6-phosphate ChEBI	+	assimilation
[Ref.: #68248]	D-galactonic acid lactone ChEBI	-	assimilation
[Ref.: #68248]	D-galactose ChEBI	+	assimilation
[Ref.: #68248]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #68248]	D-gluconate ChEBI	+/-	assimilation
[Ref.: #68248]	D-glucose 6-phosphate ChEBI	+/-	assimilation
[Ref.: #68248]	D-glucuronate ChEBI	-	assimilation
[Ref.: #68248]	D-lactic acid methyl ester	-	assimilation
[Ref.: #68248]	D-malate ChEBI	-	assimilation
[Ref.: #68248]	D-saccharate ChEBI	-	assimilation
[Ref.: #68248]	D-salicin ChEBI	-	assimilation
[Ref.: #68248]	D-serine ChEBI	-	assimilation
[Ref.: #68248]	formate ChEBI	-	assimilation
[Ref.: #68248]	gelatin ChEBI	-	assimilation
[Ref.: #68248]	gentiobiose ChEBI	-	assimilation
[Ref.: #68248]	glucuronamide ChEBI	+	assimilation
[Ref.: #68248]	glycyl-L-proline ChEBI	-	assimilation
[Ref.: #68248]	L-arginine ChEBI	-	assimilation
[Ref.: #68248]	L-glutamate ChEBI	-	assimilation
[Ref.: #68248]	L-histidine ChEBI	-	assimilation
[Ref.: #68248]	L-lactate	-	assimilation
[Ref.: #68248]	L-malate ChEBI	+/-	assimilation
[Ref.: #68248]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #68248]	L-rhamnose ChEBI	-	assimilation
[Ref.: #68248]	L-serine ChEBI	-	assimilation
[Ref.: #68248]	maltose ChEBI	+	assimilation
[Ref.: #68248]	melibiose ChEBI	+	assimilation
[Ref.: #68248]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #68248]	methyl pyruvate ChEBI	-	assimilation
[Ref.: #68248]	N-acetyl-beta-D-mannosamine ChEBI	-	assimilation
[Ref.: #68248]	n-acetyl-D-galactosamine ChEBI	-	assimilation
[Ref.: #68248]	N-acetyl-D-glucosamine ChEBI	-	assimilation
[Ref.: #68248]	n-acetyl-neuraminic acid	-	assimilation
[Ref.: #68248]	pectin ChEBI	+	assimilation
[Ref.: #68248]	stachyose ChEBI	+/-	assimilation
[Ref.: #68248]	sucrose ChEBI	+	assimilation
[Ref.: #68248]	trehalose ChEBI	+/-	assimilation
[Ref.: #68248]	turanose ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68248]

Fatty acid	Percentage
C15:0 anteiso	35.9
C14:0 iso	30.8
C14:0	8.4
C16:0 iso	7.9
C16:0	7
C13:0 anteiso	3.5
C15:1 anteiso A	1.6
C15:0 iso	1.6
C13:0	0.9
C17:0 anteiso	0.8
C12:0 iso	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66530]	Sample type/isolated from	freshwater
[Ref.: #66530]	Geographic location (country and/or sea, region)	Leopoldskronerweiher, Salzburg
[Ref.: #66530]	Country	Austria
[Ref.: #66530]	Country ISO 3 Code	AUT
[Ref.: #66530]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Surface water from the Leopoldskroner Weiher, a small artificial freshwater pond
[Ref.: #67770]	Geographic location (country and/or sea, region)	Salzburg
[Ref.: #67770]	Country	Austria
[Ref.: #67770]	Country ISO 3 Code	AUT
[Ref.: #67770]	Continent	Europe

[Ref.: #68248]	Sample type/isolated from	watersurface
[Ref.: #68248]	Sampling date	2017-11-01
[Ref.: #68248]	Geographic location (country and/or sea, region)	Leopoldskroner Weiher, Salzburg
[Ref.: #68248]	Country	Austria
[Ref.: #68248]	Country ISO 3 Code	AUT
[Ref.: #68248]	Continent	Europe
[Ref.: #68248]	Geographic location	47.7883°/13.0367°
[Ref.: #68248]	Enrichment culture	NSY agar
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MK951675 (>99% sequence identity) for Rhodoluna limnophila subclade from Microbeatlas

Aquatic Samples	2284
Soil Samples	110
Animal Samples	677
Plant Samples	16
Total Samples	3087

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66530]	Rhodoluna limnophila strain 27D-LEPI 16S ribosomal RNA gene, partial sequence	MK951675	1386	GenBank ENA	232537 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rhodoluna limnophila 27D-LEPI	GCA_005845365	complete	GenBank ENA	232537 tax ID	*

[Ref.: #68248]	GC-content	54 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	53.7 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhodoluna limnophila 27D-LEPI NCBI: GCA_005845365.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	84.123	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	90.197	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	66.389	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	90.053	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	86.465	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93.5	no

Availability in culture collections External links

[Ref.: #66530]

Culture collection no.

DSM 107804, JCM 32926

[Ref.: #112150]

SI-ID 398667

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhodoluna limnophila sp. nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.	Pitt A, Schmidt J, Koll U, Hahn MW	Int J Syst Evol Microbiol	10.1099/ijsem.0.003720	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66530

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107804

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68248

Alexandra Pitt, Johanna Schmidt, Ulrike Koll and Martin W. Hahn: Rhodoluna limnophila sp. nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria. IJSEM 69: 3946 - 3954 2019 ( DOI 10.1099/ijsem.0.003720 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#112150

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398667.1 )

* These data were automatically processed and therefore are not curated

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