Rhodoluna limnophila (DSM 107804, JCM 32926, 27D-LEPI)

Name: Rhodoluna limnophila (DSM 107804, JCM 32926, 27D-LEPI)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 159679

Type strain

Strain Designation 27D-LEPI

Culture col. no. DSM 107804 JCM 32926 27D-LEPI

NCBI tax ID(s) 232537

Special Collections DSMZ JCM Literature strains

Links

Rhodoluna limnophila 27D-LEPI is an aerobe, chemoorganotroph, psychrophilic prokaryote that forms circular colonies and was isolated from freshwater.

Gram-positive rod-shaped colony-forming aerobe chemoorganotroph psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 398667

JCM 32926,DSM 107804

@ref 20215

Last LPSN update 2025-12-16 10:08:19

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Rhodoluna

Species Rhodoluna limnophila

Full scientific name Rhodoluna limnophila Pitt et al. 2019

Synonyms (1)

"Candidatus Rhodoluna limnophila"

BacDive ID	Other strains from **Rhodoluna limnophila** (2)	Type strain
159678	R. limnophila CM 32925, 1B-Mac, DSM 107802, JCM 32925
159680	R. limnophila 36A-HellB, DSM 107805, JCM 32927

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
68248	positive	0.5 µm	0.3 µm	rod-shaped
125438

Colony morphology

@ref	Colony color	Colony shape	Medium used
68248	light red	circular	NSY agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
66530	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
66530	positive	growth	22	psychrophilic
67770	positive	growth	25	mesophilic
68248	positive	growth	6-34

Physiology and metabolism

Oxygen tolerance

68248

Oxygen toleranceaerobe

Nutrition type

68248

Typechemoorganotroph

Spore formation

@ref	Spore formation	Confidence
125438		90.053

Halophily

@ref	Salt	Growth	Tested relation	Concentration
68248	NaCl	positive	growth	0-0.4 %(w/v)

Observation

67770

Observationquinones: MK-11

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68248	17521 ChEBI	(-)-quinic acid	-	assimilation
68248	64552 ChEBI	2-hydroxybutyrate	-	assimilation
68248	18101 ChEBI	4-hydroxyphenylacetic acid	-	assimilation
68248	13705 ChEBI	acetoacetate	+	assimilation
68248	17925 ChEBI	alpha-D-glucose	+	assimilation
68248	64552 ChEBI	alpha-hydroxybutyrate	-	assimilation
68248	8295 ChEBI	beta-hydroxybutyrate	-	assimilation
68248	73706 ChEBI	bromosuccinate	-	assimilation
68248	17057 ChEBI	cellobiose	-	assimilation
68248	16947 ChEBI	citrate	-	assimilation
68248	15824 ChEBI	D-fructose	+	assimilation
68248	78697 ChEBI	D-fructose 6-phosphate	+	assimilation
68248	15895 ChEBI	D-galactonic acid lactone	-	assimilation
68248	12936 ChEBI	D-galactose	+	assimilation
68248	18024 ChEBI	D-galacturonic acid	-	assimilation
68248	8391 ChEBI	D-gluconate	+/-	assimilation
68248	14314 ChEBI	D-glucose 6-phosphate	+/-	assimilation
68248	15748 ChEBI	D-glucuronate	-	assimilation
68248		D-lactic acid methyl ester	-	assimilation
68248	15588 ChEBI	D-malate	-	assimilation
68248	33801 ChEBI	D-saccharate	-	assimilation
68248	17814 ChEBI	D-salicin	-	assimilation
68248	16523 ChEBI	D-serine	-	assimilation
68248	15740 ChEBI	formate	-	assimilation
68248	5291 ChEBI	gelatin	-	assimilation
68248	28066 ChEBI	gentiobiose	-	assimilation
68248	32323 ChEBI	glucuronamide	+	assimilation
68248	70744 ChEBI	glycyl-L-proline	-	assimilation
68248	16467 ChEBI	L-arginine	-	assimilation
68248	29985 ChEBI	L-glutamate	-	assimilation
68248	15971 ChEBI	L-histidine	-	assimilation
68248		L-lactate	-	assimilation
68248	15589 ChEBI	L-malate	+/-	assimilation
68248	18183 ChEBI	L-pyroglutamic acid	-	assimilation
68248	62345 ChEBI	L-rhamnose	-	assimilation
68248	17115 ChEBI	L-serine	-	assimilation
68248	17306 ChEBI	maltose	+	assimilation
68248	28053 ChEBI	melibiose	+	assimilation
68248	37657 ChEBI	methyl D-glucoside	-	assimilation
68248	51850 ChEBI	methyl pyruvate	-	assimilation
68248	63154 ChEBI	N-acetyl-beta-D-mannosamine	-	assimilation
68248	28037 ChEBI	n-acetyl-D-galactosamine	-	assimilation
68248	506227 ChEBI	N-acetyl-D-glucosamine	-	assimilation
68248		n-acetyl-neuraminic acid	-	assimilation
68248	17309 ChEBI	pectin	+	assimilation
68248	17164 ChEBI	stachyose	+/-	assimilation
68248	17992 ChEBI	sucrose	+	assimilation
68248	27082 ChEBI	trehalose	+/-	assimilation
68248	32528 ChEBI	turanose	+	assimilation

Fatty acid profile

Metadata FA analysis

@ref

68248

Fatty acid	Percentage
C15:0 anteiso	35.9
C14:0 iso	30.8
C14:0	8.4
C16:0 iso	7.9
C16:0	7
C13:0 anteiso	3.5
C15:1 anteiso A	1.6
C15:0 iso	1.6
C13:0	0.9
C17:0 anteiso	0.8
C12:0 iso	0.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
66530	freshwater	Leopoldskronerweiher, Salzburg	Austria	AUT	Europe
67770	Surface water from the Leopoldskroner Weiher, a small artificial freshwater pond	Salzburg	Austria	AUT	Europe
68248	watersurface	Leopoldskroner Weiher, Salzburg	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence MK951675 (>99% sequence identity) for Rhodoluna limnophila subclade from Microbeatlas

Aquatic Samples	2284
Soil Samples	110
Animal Samples	677
Plant Samples	16
Total Samples	3087

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM584536v1 assembly for Rhodoluna limnophila 27D-LEPI	complete	GCA_005845365	232537.4	2816332122	232537	98.49

Genome browser: GCA_005845365

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66530	Rhodoluna limnophila strain 27D-LEPI 16S ribosomal RNA gene, partial sequence	MK951675	1386		232537

GC content

@ref	GC-content (mol%)	Method
68248	54	genome sequence analysis
67770	53.7	genome sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Rhodoluna limnophila 27D-LEPI
NCBI: GCA_005845365.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.12	no
125438	anaerobic	anaerobicⓘ	no	90.20	yes
125438	aerobic	aerobicⓘ	yes	66.39	yes
125438	spore-forming	spore-formingⓘ	no	90.05	no
125438	thermophilic	thermophileⓘ	no	86.47	no
125438	flagellated	motile2+ⓘ	no	93.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhodoluna limnophila sp. nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria.	Pitt A, Schmidt J, Koll U, Hahn MW	Int J Syst Evol Microbiol	10.1099/ijsem.0.003720	2019

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66530	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107804
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68248	Alexandra Pitt, Johanna Schmidt, Ulrike Koll and Martin W. Hahn: Rhodoluna limnophila sp. nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria. IJSEM 69: 3946 - 3954 2019 ( DOI 10.1099/ijsem.0.003720 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rhodoluna limnophila (DSM 107804, JCM 32926, 27D-LEPI)

Name and taxonomic classification