Acidithiobacillus sulfuriphilus (DSM 105150, KCTC 4683, CJ-2)

Name: Acidithiobacillus sulfuriphilus (DSM 105150, KCTC 4683, CJ-2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 159192

Type strain

Strain Designation CJ-2

Culture col. no. DSM 105150 KCTC 4683 CJ-2

NCBI tax ID(s) 1867749

Special Collections DSMZ KCTC Literature strains

Links

Acidithiobacillus sulfuriphilus CJ-2 is an aerobe, chemolithoautotroph, mesophilic prokaryote that forms circular colonies and was isolated from mine-drainage sample.

motile rod-shaped colony-forming aerobe chemolithoautotroph mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 401630

KCTC 4683,DSM 105150

@ref 20215

Last LPSN update 2025-12-16 10:08:12

Domain Pseudomonadati

Phylum Pseudomonadota

Class Acidithiobacillia

Order Acidithiobacillales

Family Acidithiobacillaceae

Genus Acidithiobacillus

Species Acidithiobacillus sulfuriphilus

Full scientific name Acidithiobacillus sulfuriphilus Falagán et al. 2019

Synonyms (1)

"Ambacidithiobacillus sulfuriphilus"

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
67771				rod-shaped
67771	negative
68181	negative	1.5-2.5 µm	0.5 µm	rod-shaped
125439	negative				96.3
125438	negative				97.708

Colony morphology

@ref	Colony color	Colony shape
68181	white	circular

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
66015	ACIDITHIOBACILLUS MEDIUM (DSMZ Medium 35)		Medium recipe at MediaDive	Name: ACIDITHIOBACILLUS MEDIUM (DSMZ Medium 35) Composition: Sulfur 10.0 g/l KH2PO4 3.0 g/l CaCl2 x 2 H2O 0.14 g/l MgCl2 x 6 H2O 0.1 g/l NH4Cl 0.1 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
66015	positive	growth	28	mesophilic
67771	positive	growth	30	mesophilic
68181	positive	optimum	25-28	mesophilic
68181	positive	growth	15-30

Culture pH

@ref	Ability	Type	PH	PH range
68181	positive	optimum	3	acidophile
68181	positive	growth	1.8-7.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
67771	aerobe
68181	obligate aerobe

Nutrition type

68181

Typechemolithoautotroph

Spore formation

@ref	Type of spore	Spore formation	Confidence
68181	endospore
125439			93.9

Observation

@ref	Observation
67771	quinones: Q8
68181	Usage of organic carbon as a carbon source
68181	uses zerovalent sulfur and reduced inorganic sulfur anions as electron donors

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68181	18276 ChEBI	dihydrogen	-	electron donor
68181	33403 ChEBI	elemental sulfur	+	electron donor
68181	29033 ChEBI	ferrous ion	-	electron donor
67964	17306 ChEBI	maltose	-	assimilation
68181	86471 ChEBI	pyrite	-	electron donor
68181	15226 ChEBI	tetrathionate	+	electron donor
68181	15226 ChEBI	tetrathionate	-	electron donor
68181	16094 ChEBI	thiosulfate	+	electron donor

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Other	#Mine

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature
66015	mine-drainage sample		North Wales, Catherine and Jane consols lead mine	United Kingdom	GBR	Europe	52.9497	4.0361 52.9497/4.0361
67771	From an adit draining a former lead mine (Catherine and Jane consols mine)		North Wales	United Kingdom	GBR	Europe
68181	mine-drainage sample	2016-04-01	adit that originates at the Catherine and Jane Consols lead mine	Wales	GBR	Europe	52.9498	-4.03609 52.9498/-4.03609	solid medium	containing ferrous iron, potassium tetrathionate and tryptone soya broth	3 weeks	30

Taxonmaps

69479

Global distribution of 16S sequence MK193868 (>99% sequence identity) for Acidithiobacillus sulfuriphilus subclade from Microbeatlas

Aquatic Samples	496
Soil Samples	130
Animal Samples	16
Plant Samples	2
Total Samples	644

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
68181	CJ2_v4 assembly for Acidithiobacillus sulfuriphilus CJ-2	complete	GCA_003721225	1867749.3	2840828123	1867749	92.84

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66015	Acidithiobacillus sulfuriphilus 16S ribosomal RNA gene, partial sequence	KX426303	1187		1867749
68181	Acidithiobacillus sulfuriphilus strain CJ-2 16S ribosomal RNA gene, partial sequence	MK193868	1530		1867749

GC content

@ref	GC-content (mol%)	Method
66015	61.5	sequence analysis
68181	61.5	genome sequence analysis
67771	61.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acidithiobacillus sulfuriphilus CJ-2
NCBI: GCA_003721225

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	93.90	no
125439	motility	BacteriaNetⓘ	yes	65.40	no
125439	gram_stain	BacteriaNetⓘ	negative	96.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	89.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acidithiobacillus sp. CJ-2
PATRIC: 1867749.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.71	yes
125438	anaerobic	anaerobicⓘ	no	80.52	yes
125438	spore-forming	spore-formingⓘ	no	89.74	yes
125438	aerobic	aerobicⓘ	yes	55.44	yes
125438	thermophilic	thermophileⓘ	no	86.35	yes
125438	flagellated	motile2+ⓘ	yes	55.23	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment.	Falagan C, Moya-Beltran A, Castro M, Quatrini R, Johnson DB	Int J Syst Evol Microbiol	10.1099/ijsem.0.003576	2019

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66015	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105150
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#67964	Yunyun Huo, Wenjun Ton, Juanjuan Wang, Fang Wang, Wenqing Bai, Entao Wang, Peng Shi, Weimin Chen, Gehong Wei: Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China. IJSEM 69: 2049 - 2056 2019 ( DOI 10.1099/ijsem.0.003430 )
#68181	Carmen Falagán1, Ana Moya-Beltrán, Matías Castro, Raquel Quatrini, D. Barrie Johnson: Acidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment. IJSEM 69: 2907 - 2913 2019 ( DOI 10.1099/ijsem.0.003576 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acidithiobacillus sulfuriphilus (DSM 105150, KCTC 4683, CJ-2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003721225

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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