Name: Acidithiobacillus sulfuriphilus CJ-2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159192

Type strain:

Species: Acidithiobacillus sulfuriphilus

Strain Designation: CJ-2

Culture col. no.: DSM 105150, KCTC 4683

Strain history: <- Carmen Falagan, Bangor Univ.

NCBI tax ID(s): 1867749 (species)

SI-ID 401630

DSM 105150

Special Collections

Section

Acidithiobacillus sulfuriphilus CJ-2 is an aerobe, chemolithoautotroph, motile bacterium that forms circular colonies and was isolated from mine-drainage sample.

colony-forming
motile
rod-shaped
aerobe
chemolithoautotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Acidithiobacillia*
	Order	*Acidithiobacillales*
	Family	*Acidithiobacillaceae*
	Genus	*Acidithiobacillus*
	Species	**Acidithiobacillus sulfuriphilus**
	Full Scientific Name (LPSN)	Acidithiobacillus sulfuriphilus Falagán et al. 2019

Synonym

"Ambacidithiobacillus sulfuriphilus"

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	negative
[Ref.: #68181]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %97.708

[Ref.: #68181]	Cell length	1.5-2.5 µm

[Ref.: #68181]	Cell width	0.5 µm

[Ref.: #68181]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #68181]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #68181]	Colony color	white
[Ref.: #68181]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66015]

Culture medium

ACIDITHIOBACILLUS MEDIUM (DSMZ Medium 35)

[Ref.: #66015]

Culture medium growth

[Ref.: #66015]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66015]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #66015]	growth	28 °C
[Ref.: #67771]	growth	30 °C
[Ref.: #68181]	optimum	25-28 °C
[Ref.: #68181]	growth	15-30 °C

	pH	Kind of pH		pH
[Ref.: #68181]			optimum	3
[Ref.: #68181]			growth	1.8-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #68181]	Oxygen tolerance	obligate aerobe

[Ref.: #68181]

Nutrition type

chemolithoautotroph

[Ref.: #68181]	Type of spore	endospore
[Ref.: #68181]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %98

[Ref.: #67771]	Observation	quinones: Q8
[Ref.: #68181]	Observation	Usage of organic carbon as a carbon source
[Ref.: #68181]	Observation	uses zerovalent sulfur and reduced inorganic sulfur anions as electron donors

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68181]	dihydrogen ChEBI	-	electron donor
[Ref.: #68181]	elemental sulfur ChEBI	+	electron donor
[Ref.: #68181]	ferrous ion ChEBI	-	electron donor
[Ref.: #67964]	maltose ChEBI	-	assimilation
[Ref.: #68181]	pyrite ChEBI	-	electron donor
[Ref.: #68181]	tetrathionate ChEBI	+	electron donor
[Ref.: #68181]	tetrathionate ChEBI	-	electron donor
[Ref.: #68181]	thiosulfate ChEBI	+	electron donor

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66015]	Sample type/isolated from	mine-drainage sample
[Ref.: #66015]	Geographic location (country and/or sea, region)	North Wales, Catherine and Jane consols lead mine
[Ref.: #66015]	Country	United Kingdom
[Ref.: #66015]	Country ISO 3 Code	GBR
[Ref.: #66015]	Continent	Europe
[Ref.: #66015]	Geographic location	52.9497°/4.0361°

[Ref.: #67771]	Sample type/isolated from	From an adit draining a former lead mine (Catherine and Jane consols mine)
[Ref.: #67771]	Geographic location (country and/or sea, region)	North Wales
[Ref.: #67771]	Country	United Kingdom
[Ref.: #67771]	Country ISO 3 Code	GBR
[Ref.: #67771]	Continent	Europe

[Ref.: #68181]	Sample type/isolated from	mine-drainage sample
[Ref.: #68181]	Sampling date	2016-04-01
[Ref.: #68181]	Geographic location (country and/or sea, region)	adit that originates at the Catherine and Jane Consols lead mine
[Ref.: #68181]	Country	Wales
[Ref.: #68181]	Country ISO 3 Code	GBR
[Ref.: #68181]	Continent	Europe
[Ref.: #68181]	Geographic location	52.9498°/-4.0361°
[Ref.: #68181]	Enrichment culture	solid medium
[Ref.: #68181]	Enrichment culture composition	containing ferrous iron, potassium tetrathionate and tryptone soya broth
[Ref.: #68181]	Enrichment culture duration	3 weeks
[Ref.: #68181]	Enrichment culture temperature	30 ̊C
* marker position based on {}

Isolation sources categories

#Engineered

#Other

#Mine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MK193868 (>99% sequence identity) for Acidithiobacillus sulfuriphilus subclade from Microbeatlas

Aquatic Samples	496
Soil Samples	130
Animal Samples	16
Plant Samples	2
Total Samples	644

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66015]	Acidithiobacillus sulfuriphilus 16S ribosomal RNA gene, partial sequence	KX426303	1187	GenBank ENA	1867749 tax ID
[Ref.: #68181]	Acidithiobacillus sulfuriphilus strain CJ-2 16S ribosomal RNA gene, partial sequence	MK193868	1530	GenBank ENA	1867749 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68181]	Acidithiobacillus sulfuriphilus CJ-2	GCA_003721225	contig	GenBank ENA	1867749 tax ID
[Ref.: #66792]	Acidithiobacillus sp. CJ-2	1867749.3	wgs	PATRIC	1867749 tax ID	*
[Ref.: #66792]	Acidithiobacillus sulfuriphilus CJ-2	2840828123	draft	JGI IMG/M	1867749 tax ID	*

[Ref.: #66015]	GC-content	61.5 mol%	sequence analysis
[Ref.: #68181]	GC-content	61.5 mol%	genome sequence analysis
[Ref.: #67771]	GC-content	61.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Acidithiobacillus sp. CJ-2 PATRIC: 1867749.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	98	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Acidithiobacillus sp. CJ-2 PATRIC: 1867749.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	97.708	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	80.521	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.737	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	55.438	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	86.354	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	55.234	no

Availability in culture collections External links

[Ref.: #66015]

Culture collection no.

DSM 105150, KCTC 4683

[Ref.: #111694]

SI-ID 401630

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment.	Falagan C, Moya-Beltran A, Castro M, Quatrini R, Johnson DB	Int J Syst Evol Microbiol	10.1099/ijsem.0.003576	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66015

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105150

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#67964

Yunyun Huo, Wenjun Ton, Juanjuan Wang, Fang Wang, Wenqing Bai, Entao Wang, Peng Shi, Weimin Chen, Gehong Wei: Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China. IJSEM 69: 2049 - 2056 2019 ( DOI 10.1099/ijsem.0.003430 )

#68181

Carmen Falagán1, Ana Moya-Beltrán, Matías Castro, Raquel Quatrini, D. Barrie Johnson: Acidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment. IJSEM 69: 2907 - 2913 2019 ( DOI 10.1099/ijsem.0.003576 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111694

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401630.1 )

* These data were automatically processed and therefore are not curated

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Acidithiobacillus sp. CJ-2

Acidithiobacillus sp. CJ-2

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