Strain identifier

BacDive ID: 159192

Type strain: Yes

Species: Acidithiobacillus sulfuriphilus

Strain Designation: CJ-2

Strain history: <- Carmen Falagan, Bangor Univ.

NCBI tax ID(s): 1867749 (species)

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General

@ref: 66015

BacDive-ID: 159192

DSM-Number: 105150

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemolithoautotroph, mesophilic, motile, rod-shaped, colony-forming

description: Acidithiobacillus sulfuriphilus CJ-2 is an aerobe, chemolithoautotroph, mesophilic bacterium that forms circular colonies and was isolated from mine-drainage sample.

NCBI tax id

  • NCBI tax id: 1867749
  • Matching level: species

strain history

@refhistory
66015<- C. Falagan, Bangor Univ., Wales, U.K.; CJ-2
67771<- Carmen Falagan, Bangor Univ.

doi: 10.13145/bacdive159192.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Acidithiobacillia
  • order: Acidithiobacillales
  • family: Acidithiobacillaceae
  • genus: Acidithiobacillus
  • species: Acidithiobacillus sulfuriphilus
  • full scientific name: Acidithiobacillus sulfuriphilus Falagán et al. 2019

  • synonyms

    • @ref: 20215
    • synonym: Ambacidithiobacillus sulfuriphilus

@ref: 66015

domain: Bacteria

phylum: Proteobacteria

class: Acidithiobacillia

order: Acidithiobacillales

family: Acidithiobacillaceae

genus: Acidithiobacillus

species: Acidithiobacillus sulfuriphilus

full scientific name: Acidithiobacillus sulfuriphilus Falagán et al. 2019

strain designation: CJ-2

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram staincell lengthcell widthconfidence
67771rod-shapedyes
67771negative
68181rod-shapedyesnegative1.5-2.5 µm0.5 µm
125439negative96.3
125438negative97.708

colony morphology

  • @ref: 68181
  • colony color: white
  • colony shape: circular

Culture and growth conditions

culture medium

  • @ref: 66015
  • name: ACIDITHIOBACILLUS MEDIUM (DSMZ Medium 35)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/35
  • composition: Name: ACIDITHIOBACILLUS MEDIUM (DSMZ Medium 35) Composition: Sulfur 10.0 g/l KH2PO4 3.0 g/l CaCl2 x 2 H2O 0.14 g/l MgCl2 x 6 H2O 0.1 g/l NH4Cl 0.1 g/l Distilled water

culture temp

@refgrowthtypetemperature
66015positivegrowth28
67771positivegrowth30
68181positiveoptimum25-28
68181positivegrowth15-30

culture pH

@refabilitytypepHPH range
68181positiveoptimum3acidophile
68181positivegrowth1.8-7.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
67771aerobe
68181obligate aerobe

nutrition type

  • @ref: 68181
  • type: chemolithoautotroph

spore formation

@reftype of sporespore formationconfidence
68181endosporeno
125439no93.9

observation

@refobservation
67771quinones: Q8
68181Usage of organic carbon as a carbon source
68181uses zerovalent sulfur and reduced inorganic sulfur anions as electron donors

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6796417306maltose-assimilation
6818133403elemental sulfur+electron donor
6818115226tetrathionate+electron donor
6818116094thiosulfate+electron donor
6818129033ferrous ion-electron donor
6818186471pyrite-electron donor
6818118276dihydrogen-electron donor
6818115226tetrathionate-electron donor

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
66015mine-drainage sampleNorth Wales, Catherine and Jane consols lead mineUnited KingdomGBREurope52.94974.0361
67771From an adit draining a former lead mine (Catherine and Jane consols mine)North WalesUnited KingdomGBREurope
68181mine-drainage sampleadit that originates at the Catherine and Jane Consols lead mineWalesGBREurope52.9498-4.036092016-04-01solid mediumcontaining ferrous iron, potassium tetrathionate and tryptone soya broth3 weeks30

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Other
  • Cat3: #Mine

taxonmaps

  • @ref: 69479
  • File name: preview.99_8442.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_163;96_544;97_4817;98_6173;99_8442&stattab=map
  • Last taxonomy: Acidithiobacillus sulfuriphilus subclade
  • 16S sequence: MK193868
  • Sequence Identity:
  • Total samples: 644
  • soil counts: 130
  • aquatic counts: 496
  • animal counts: 16
  • plant counts: 2

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
66015Acidithiobacillus sulfuriphilus 16S ribosomal RNA gene, partial sequenceKX4263031187nuccore1867749
68181Acidithiobacillus sulfuriphilus strain CJ-2 16S ribosomal RNA gene, partial sequenceMK1938681530nuccore1867749

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acidithiobacillus sp. CJ-21867749.3wgspatric1867749
66792Acidithiobacillus sulfuriphilus CJ-22840828123draftimg1867749
68181Acidithiobacillus sulfuriphilus CJ-2GCA_003721225contigncbi1867749

GC content

@refGC-contentmethod
6601561.5sequence analysis
6818161.5genome sequence analysis
6777161.5

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno97.708yes
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no80.521yes
125438spore-formingspore-formingAbility to form endo- or exosporesno89.737yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes55.438yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno86.354yes
125438motile2+flagellatedAbility to perform flagellated movementyes55.234no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno93.9
125439BacteriaNetmotilityAbility to perform movementyes65.4
125439BacteriaNetgram_stainReaction to gram-stainingnegative96.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthanaerobe89.3

External links

@ref: 66015

culture collection no.: DSM 105150, KCTC 4683

straininfo link

  • @ref: 111694
  • straininfo: 401630

literature

  • topic: Phylogeny
  • Pubmed-ID: 31274405
  • title: Acidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment.
  • authors: Falagan C, Moya-Beltran A, Castro M, Quatrini R, Johnson DB
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003576
  • year: 2019
  • mesh: Acidithiobacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydrogen-Ion Concentration, Iron, *Mining, Nucleic Acid Hybridization, Oxidation-Reduction, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfur/*metabolism, Wales, *Water Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66015Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105150Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105150)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
67964Yunyun Huo, Wenjun Ton, Juanjuan Wang, Fang Wang, Wenqing Bai, Entao Wang, Peng Shi, Weimin Chen, Gehong WeiRhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China10.1099/ijsem.0.003430IJSEM 69: 2049-2056 2019
68181Carmen Falagán1, Ana Moya-Beltrán, Matías Castro, Raquel Quatrini, D. Barrie JohnsonAcidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment10.1099/ijsem.0.003576IJSEM 69: 2907-2913 2019
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111694Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401630.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG