Name: Flavobacterium lacicola KCTC 52571
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158799

Type strain:

Species: Flavobacterium lacicola

Culture col. no.: KCTC 52571, NBRC 112883, IMCC 25901

Strain history: <- Jang-Cheon Cho, Inha Univ.

NCBI tax ID(s): 2026655 (species)

Section

Flavobacterium lacicola KCTC 52571 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from oligotrophic freshwater lake.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	25-08-2022 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Bacteroidota
	Class	Flavobacteriia
	Order	Flavobacteriales
	Family	Flavobacteriaceae
	Genus	Flavobacterium
	Species	Flavobacterium lacicola
	Full Scientific Name (PNU)	Flavobacterium lacicola Park et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65559]	Gram stain	negative
[Ref.: #65559]	Cell length	1.0-1.5 µm
[Ref.: #65559]	Cell width	0.3-0.6 µm
[Ref.: #65559]	Cell shape	rod-shaped

[Ref.: #67771]	Gram stain	negative

[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #65559]	Colony color	orange
[Ref.: #65559]	Colony shape	circular
[Ref.: #65559]	Incubation period	5 days
[Ref.: #65559]	Cultivation medium used	R2A

[Ref.: #65559]	Pigment production	yes
[Ref.: #65559]	Pigment name	carotenoid

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65559]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #65559]

Culture medium growth

		Temperature
[Ref.: #65559]	growth	10-30 °C
[Ref.: #65559]	optimum	20 °C
[Ref.: #67771]	growth	25 °C

[Ref.: #65559]	Temperature range	psychrophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65559]			optimum	7
[Ref.: #65559]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65559]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65559]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65559]		NaCl		growth	0-1.0	%
[Ref.: #65559]		NaCl		optimum	0	%

[Ref.: #67771]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65559]	alpha-cyclodextrin ChEBI	+	oxidation
[Ref.: #65559]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #65559]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #65559]	casein	-	hydrolysis
[Ref.: #65559]	chitin ChEBI	-	hydrolysis
[Ref.: #65559]	D-galactose ChEBI	+	oxidation
[Ref.: #65559]	dextrin ChEBI	+	oxidation
[Ref.: #65559]	dna ChEBI	-	hydrolysis
[Ref.: #65559]	egg yolk	-	hydrolysis
[Ref.: #65559]	esculin ChEBI	+	hydrolysis
[Ref.: #65559]	gelatin ChEBI	-	hydrolysis
[Ref.: #65559]	glucose ChEBI	-	fermentation
[Ref.: #65559]	glucose 1-phosphate ChEBI	+	oxidation
[Ref.: #65559]	glycogen ChEBI	+	oxidation
[Ref.: #65559]	glycyl-l-glutamate ChEBI	+	oxidation
[Ref.: #65559]	lactose ChEBI	+	oxidation
[Ref.: #65559]	lactulose ChEBI	+	oxidation
[Ref.: #65559]	maltose ChEBI	+	oxidation
[Ref.: #65559]	nitrate ChEBI	-	reduction
[Ref.: #65559]	pectin ChEBI	+	hydrolysis
[Ref.: #65559]	starch ChEBI	+	hydrolysis
[Ref.: #65559]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65559]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65559]	tyrosine ChEBI	-	hydrolysis
[Ref.: #65559]	uridine ChEBI	+	oxidation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65559]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65559]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65559]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #65559]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #65559]	kanamycin ChEBI	yes	10	µg (disc)
[Ref.: #65559]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #65559]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #65559]	tetracycline ChEBI	yes	30	µg (disc)

	Metabolite production	Metabolite
[Ref.: #65559]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #65559]	acid phosphatase	+	3.1.3.2
[Ref.: #65559]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65559]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65559]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65559]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65559]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65559]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65559]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65559]	beta-galactosidase	+	3.2.1.23
[Ref.: #65559]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65559]	catalase	-	1.11.1.6
[Ref.: #65559]	cystine arylamidase	+	3.4.11.3
[Ref.: #65559]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65559]	esterase (C 4)	+
[Ref.: #65559]	esterase Lipase (C 8)	-
[Ref.: #65559]	leucine arylamidase	+	3.4.11.1
[Ref.: #65559]	lipase (C 14)	-
[Ref.: #65559]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65559]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65559]	trypsin	+	3.4.21.4
[Ref.: #65559]	urease	-	3.5.1.5
[Ref.: #65559]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65559]

Fatty acid	Percentage
C15:0 anteiso	31.3
C16:1ω6c / C16:1ω7c	19.7
C15:0 iso	13.3
C15:1 iso G	9.5
C16:0	4.1
C15:0 3OH	3.9
C14:0	2.8
C14:0 iso	2
C13:1	2
C16:0 3OH	1.9
C15:1 anteiso A	1.7
C12:0 iso	1.1
C15:0 2OH	1
C13:0 anteiso	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65559]	Sample type/isolated from	oligotrophic freshwater lake
[Ref.: #65559]	Sampling date	2016-05-12
[Ref.: #65559]	Geographic location (country and/or sea, region)	Lake Soyang
[Ref.: #65559]	Country	Republic of Korea
[Ref.: #65559]	Country ISO 3 Code	KOR
[Ref.: #65559]	Continent	Asia
[Ref.: #65559]	Geographic location	37.9475°/127.8190°
[Ref.: #65559]	Enrichment culture	R2A
[Ref.: #65559]	Enrichment culture duration	7 days
[Ref.: #65559]	Enrichment culture temperature	20 ̊C

[Ref.: #67771]	Sample type/isolated from	From freshwater lake
[Ref.: #67771]	Geographic location (country and/or sea, region)	Lake Soyang
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
[Ref.: #67771]	Geographic location	37.9475°/127.8190°

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65559]	16S rRNA gene sequence	KX505863		GenBank

[Ref.: #65559]	GC-content	34.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67771]	GC-content	34.2 mol%

Availability in culture collections External links

[Ref.: #65559]

Culture collection no.

KCTC 52571, NBRC 112883, IMCC 25901

Literature:

Title	Authors	Journal	DOI	Year	Mesh	Topic	Topic2
Flavobacterium lacicola sp. nov., isolated from a freshwater lake.	Park M, Song J, Nam GG, Kim S, Joung Y, Cho JC	Int J Syst Evol Microbiol	10.1099/ijsem.0.002712	2018	Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/classification/genetics/isolation & purification, Lakes/microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry	Phylogeny	Transcriptome	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65559

Miri Park, Jaeho Song, Gi Gyun Nam, Suhyun Kim, Yochan Joung and Jang-Cheon Cho: Flavobacterium lacicola sp. nov., isolated from a freshwater lake. IJSEM 68: 1565 - 1570 2018 ( DOI 10.1099/ijsem.0.002712 , PubMed 29537359 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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