Strain identifier

BacDive ID: 158799

Type strain: Yes

Species: Flavobacterium lacicola

Culture col. no.: KCTC 52571, NBRC 112883, IMCC 25901

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General

@ref: 65559

BacDive-ID: 158799

keywords: colony-forming, rod-shaped, gram-negative, aerobe, Bacteria, 16S sequence

description: Flavobacterium lacicola KCTC 52571 is an aerobe, gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from oligotrophic freshwater lake.

doi: 10.13145/bacdive158799.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidetes
  • domain: Bacteria
  • phylum: Bacteroidetes
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium lacicola
  • full scientific name: Flavobacterium lacicola Park et al. 2018

@ref: 65559

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium lacicola

type strain: yes

Morphology

cell morphology

  • @ref: 65559
  • gram stain: negative
  • cell length: 1.0-1.5 µm
  • cell width: 0.3-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65559
  • colony color: orange
  • colony shape: circular
  • incubation period: 5 days
  • medium used: R2A

pigmentation

  • @ref: 65559
  • production: yes
  • name: carotenoid

Culture and growth conditions

culture medium

  • @ref: 65559
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65559positivegrowth10-30
65559positiveoptimum20psychrophilic

culture pH

@refabilitytypepH
65559positivegrowth6-8
65559positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 65559
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
65559NaClpositivegrowth0-1.0 %
65559NaClpositiveoptimum0 %non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6555940585alpha-cyclodextrin+oxidation
6555917925alpha-D-glucose+oxidation
6555985146carboxymethylcellulose+hydrolysis
65559casein-hydrolysis
6555917029chitin-hydrolysis
6555912936D-galactose+oxidation
6555923652dextrin+oxidation
6555916991dna-hydrolysis
65559egg yolk-hydrolysis
655594853esculin+hydrolysis
655595291gelatin-hydrolysis
6555917234glucose-fermentation
6555929042glucose 1-phosphate+oxidation
6555928087glycogen+oxidation
6555917716lactose+oxidation
655596359lactulose+oxidation
6555917306maltose+oxidation
6555973784glycyl-l-glutamate+oxidation
6555917632nitrate-reduction
6555917309pectin+hydrolysis
6555928017starch+hydrolysis
6555953424tween 20-hydrolysis
6555953426tween 80-hydrolysis
6555918186tyrosine-hydrolysis
6555916704uridine+oxidation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6555928077rifampicinyesyes5 µg (disc)
6555928971ampicillinyesyes10 µg (disc)
6555917833gentamicinyesyes10 µg (disc)
655596104kanamycinyesyes10 µg (disc)
6555948923erythromycinyesyes15 µg (disc)
6555917698chloramphenicolyesyes30 µg (disc)
65559100147nalidixic acidyesyes30 µg (disc)
6555927902tetracyclineyesyes30 µg (disc)

metabolite production

  • @ref: 65559
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65559cytochrome oxidase+1.9.3.1
65559catalase-1.11.1.6
65559beta-galactosidase+3.2.1.23
65559arginine dihydrolase-3.5.3.6
65559urease-3.5.1.5
65559alkaline phosphatase+3.1.3.1
65559esterase (C 4)+
65559leucine arylamidase+3.4.11.1
65559cystine arylamidase+3.4.11.3
65559trypsin+3.4.21.4
65559acid phosphatase+3.1.3.2
65559naphthol-AS-BI-phosphohydrolase+
65559esterase Lipase (C 8)-
65559lipase (C 14)-
65559valine arylamidase-
65559alpha-chymotrypsin-3.4.21.1
65559alpha-galactosidase-3.2.1.22
65559beta-glucuronidase-3.2.1.31
65559alpha-glucosidase-3.2.1.20
65559N-acetyl-beta-glucosaminidase-3.2.1.52
65559alpha-mannosidase-3.2.1.24
65559alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65559C14:02.8
    65559C16:04.1
    65559C13:12
    65559C12:0 iso1.1
    65559C14:0 iso2
    65559C15:0 iso13.3
    65559C15:1 iso G9.5
    65559C13:0 anteiso1
    65559C15:0 anteiso31.3
    65559C15:1 anteiso A1.7
    65559C15:0 2OH1
    65559C15:0 3OH3.9
    65559C16:0 3OH1.9
    65559C16:1ω6c / C16:1ω7c19.7
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 20
  • incubation time: 5
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 65559
  • sample type: oligotrophic freshwater lake
  • sampling date: 2016-05-12
  • geographic location: Lake Soyang
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 37.9475
  • longitude: 127.819
  • enrichment culture: R2A
  • enrichment culture duration: 7 days
  • enrichment culture temperature: 20

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Lake (large)

Sequence information

16S sequences

  • @ref: 65559
  • description: 16S rRNA gene sequence
  • accession: KX505863
  • database: nuccore

GC content

  • @ref: 65559
  • GC-content: 34.2
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 65559

culture collection no.: KCTC 52571, NBRC 112883, IMCC 25901

Reference

@idauthorstitledoi/urljournal
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
65559Miri Park, Jaeho Song, Gi Gyun Nam, Suhyun Kim, Yochan Joung and Jang-Cheon ChoFlavobacterium lacicola sp. nov., isolated from a freshwater lake10.1099/ijsem.0.002712IJSEM 68: 1565-1570 2018