Strain identifier
BacDive ID: 158799
Type strain:
Species: Flavobacterium lacicola
Strain history: <- Jang-Cheon Cho, Inha Univ.
NCBI tax ID(s): 2026655 (species)
version 8.1 (current version)
General
@ref: 65559
BacDive-ID: 158799
keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming
description: Flavobacterium lacicola KCTC 52571 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from oligotrophic freshwater lake.
NCBI tax id
- NCBI tax id: 2026655
- Matching level: species
strain history
- @ref: 67771
- history: <- Jang-Cheon Cho, Inha Univ.
doi: 10.13145/bacdive158799.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Flavobacterium
- species: Flavobacterium lacicola
- full scientific name: Flavobacterium lacicola Park et al. 2018
@ref: 65559
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Flavobacterium
species: Flavobacterium lacicola
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
65559 | negative | 1.0-1.5 µm | 0.3-0.6 µm | rod-shaped | no |
67771 | rod-shaped | no | |||
67771 | negative |
colony morphology
- @ref: 65559
- colony color: orange
- colony shape: circular
- incubation period: 5 days
- medium used: R2A
pigmentation
- @ref: 65559
- production: yes
- name: carotenoid
Culture and growth conditions
culture medium
- @ref: 65559
- name: Reasoner's 2A agar (R2A)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65559 | positive | growth | 10-30 | |
65559 | positive | optimum | 20 | psychrophilic |
67771 | positive | growth | 25 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
65559 | positive | growth | 6-8 |
65559 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
65559 | aerobe |
67771 | aerobe |
halophily
@ref | salt | growth | tested relation | concentration | halophily level |
---|---|---|---|---|---|
65559 | NaCl | positive | growth | 0-1.0 % | |
65559 | NaCl | positive | optimum | 0 % | non-halophilic |
observation
- @ref: 67771
- observation: quinones: MK-6
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65559 | 40585 | alpha-cyclodextrin | + | oxidation |
65559 | 17925 | alpha-D-glucose | + | oxidation |
65559 | 85146 | carboxymethylcellulose | + | hydrolysis |
65559 | casein | - | hydrolysis | |
65559 | 17029 | chitin | - | hydrolysis |
65559 | 12936 | D-galactose | + | oxidation |
65559 | 23652 | dextrin | + | oxidation |
65559 | 16991 | dna | - | hydrolysis |
65559 | egg yolk | - | hydrolysis | |
65559 | 4853 | esculin | + | hydrolysis |
65559 | 5291 | gelatin | - | hydrolysis |
65559 | 17234 | glucose | - | fermentation |
65559 | 29042 | glucose 1-phosphate | + | oxidation |
65559 | 28087 | glycogen | + | oxidation |
65559 | 17716 | lactose | + | oxidation |
65559 | 6359 | lactulose | + | oxidation |
65559 | 17306 | maltose | + | oxidation |
65559 | 73784 | glycyl-l-glutamate | + | oxidation |
65559 | 17632 | nitrate | - | reduction |
65559 | 17309 | pectin | + | hydrolysis |
65559 | 28017 | starch | + | hydrolysis |
65559 | 53424 | tween 20 | - | hydrolysis |
65559 | 53426 | tween 80 | - | hydrolysis |
65559 | 18186 | tyrosine | - | hydrolysis |
65559 | 16704 | uridine | + | oxidation |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. |
---|---|---|---|---|---|
65559 | 28077 | rifampicin | yes | yes | 5 µg (disc) |
65559 | 28971 | ampicillin | yes | yes | 10 µg (disc) |
65559 | 17833 | gentamicin | yes | yes | 10 µg (disc) |
65559 | 6104 | kanamycin | yes | yes | 10 µg (disc) |
65559 | 48923 | erythromycin | yes | yes | 15 µg (disc) |
65559 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) |
65559 | 100147 | nalidixic acid | yes | yes | 30 µg (disc) |
65559 | 27902 | tetracycline | yes | yes | 30 µg (disc) |
metabolite production
- @ref: 65559
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
65559 | cytochrome oxidase | + | 1.9.3.1 |
65559 | catalase | - | 1.11.1.6 |
65559 | beta-galactosidase | + | 3.2.1.23 |
65559 | arginine dihydrolase | - | 3.5.3.6 |
65559 | urease | - | 3.5.1.5 |
65559 | alkaline phosphatase | + | 3.1.3.1 |
65559 | esterase (C 4) | + | |
65559 | leucine arylamidase | + | 3.4.11.1 |
65559 | cystine arylamidase | + | 3.4.11.3 |
65559 | trypsin | + | 3.4.21.4 |
65559 | acid phosphatase | + | 3.1.3.2 |
65559 | naphthol-AS-BI-phosphohydrolase | + | |
65559 | esterase Lipase (C 8) | - | |
65559 | lipase (C 14) | - | |
65559 | valine arylamidase | - | |
65559 | alpha-chymotrypsin | - | 3.4.21.1 |
65559 | alpha-galactosidase | - | 3.2.1.22 |
65559 | beta-glucuronidase | - | 3.2.1.31 |
65559 | alpha-glucosidase | - | 3.2.1.20 |
65559 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65559 | alpha-mannosidase | - | 3.2.1.24 |
65559 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage 65559 C14:0 2.8 65559 C16:0 4.1 65559 C13:1 2 65559 C12:0 iso 1.1 65559 C14:0 iso 2 65559 C15:0 iso 13.3 65559 C15:1 iso G 9.5 65559 C13:0 anteiso 1 65559 C15:0 anteiso 31.3 65559 C15:1 anteiso A 1.7 65559 C15:0 2OH 1 65559 C15:0 3OH 3.9 65559 C16:0 3OH 1.9 65559 C16:1ω6c / C16:1ω7c 19.7 - type of FA analysis: whole cell analysis
- incubation medium: R2A
- incubation temperature: 20
- incubation time: 5
- library/peak naming table: TSBA6
- system: MIS MIDI
- method/protocol: Sasser, 1990
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | sampling date | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|---|---|
65559 | oligotrophic freshwater lake | 2016-05-12 | Lake Soyang | Republic of Korea | KOR | Asia | 37.9475 | 127.819 | R2A | 7 days | 20 |
67771 | From freshwater lake | Lake Soyang | Republic of Korea | KOR | Asia | 37.9475 | 127.819 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Freshwater |
#Environmental | #Aquatic | #Lake (large) |
Sequence information
16S sequences
- @ref: 65559
- description: 16S rRNA gene sequence
- accession: KX505863
- database: nuccore
GC content
@ref | GC-content | method |
---|---|---|
65559 | 34.2 | thermal denaturation, midpoint method (Tm) |
67771 | 34.2 |
External links
@ref: 65559
culture collection no.: KCTC 52571, NBRC 112883, IMCC 25901
literature
- topic: Phylogeny
- Pubmed-ID: 29537359
- title: Flavobacterium lacicola sp. nov., isolated from a freshwater lake.
- authors: Park M, Song J, Nam GG, Kim S, Joung Y, Cho JC
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002712
- year: 2018
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Lakes/*microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65559 | Miri Park, Jaeho Song, Gi Gyun Nam, Suhyun Kim, Yochan Joung and Jang-Cheon Cho | Flavobacterium lacicola sp. nov., isolated from a freshwater lake | 10.1099/ijsem.0.002712 | IJSEM 68: 1565-1570 2018 | 29537359 |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc |