Name: Companilactobacillus metriopterae Hime5-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158793

Type strain:

Species: Companilactobacillus metriopterae

Strain Designation: Hime5-1

Culture col. no.: JCM 31635, DSM 103730, Hime 5-1

Strain history: S. Kawasaki; Dept. of Biosci., Tokyo Univ. of Agric., Japan; Hime5-1.

NCBI tax ID(s): 1909267 (species)

Section

Companilactobacillus metriopterae Hime5-1 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from gut of grasshopper Metrioptera engelhardti.

Gram-positive
rod-shaped
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	25-08-2022 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Bacillota
	Class	Bacilli
	Order	Lactobacillales
	Family	Lactobacillaceae
	Genus	Companilactobacillus
	Species	Companilactobacillus metriopterae
	Full Scientific Name (PNU)	Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020

	Synonym	Lactobacillus metriopterae
	Synonym	Lactobacillus terrae

Information on morphological and physiological properties Morphology

[Ref.: #65552]	Gram stain	positive
[Ref.: #65552]	Cell length	0.6-1.2 µm
[Ref.: #65552]	Cell width	0.2 µm
[Ref.: #65552]	Cell shape	rod-shaped

[Ref.: #65552]	Colony size	1-2 mm
[Ref.: #65552]	Colony color	yellowish
[Ref.: #65552]	Incubation period	2 days
[Ref.: #65552]	Cultivation medium used	MRS agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65552]

Culture medium

MRS agar

[Ref.: #65552]

Culture medium growth

[Ref.: #67232]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #67232]

Culture medium growth

[Ref.: #67232]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67232]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #65552]		growth	15-37 °C
[Ref.: #65552]	no	growth	10 °C
[Ref.: #65552]	no	growth	45 °C
[Ref.: #67232]		growth	30 °C
[Ref.: #67770]		growth	30 °C

[Ref.: #65552]	Temperature range	psychrophilic
[Ref.: #65552]	Temperature range	thermophilic
[Ref.: #67232]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65552]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65552]		NaCl		growth	7.0	%(w/v)
[Ref.: #65552]		NaCl	no	growth	7.5	%(w/v)

[Ref.: #65552]	Observation	singly, in pairs or short chains
[Ref.: #65552]	Observation	homofermentative

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65552]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #65552]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #65552]	amygdalin ChEBI	+/-	builds acid from
[Ref.: #65552]	arbutin ChEBI	-	builds acid from
[Ref.: #65552]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #65552]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65552]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65552]	D-fructose ChEBI	+	builds acid from
[Ref.: #65552]	D-fucose ChEBI	-	builds acid from
[Ref.: #65552]	D-galactose ChEBI	+/-	builds acid from
[Ref.: #65552]	D-glucose ChEBI	+	builds acid from
[Ref.: #65552]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65552]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65552]	D-mannose ChEBI	+	builds acid from
[Ref.: #65552]	D-ribose ChEBI	+	builds acid from
[Ref.: #65552]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65552]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65552]	D-xylose ChEBI	-	builds acid from
[Ref.: #65552]	erythritol ChEBI	-	builds acid from
[Ref.: #65552]	esculin ChEBI	+	builds acid from
[Ref.: #65552]	galactitol ChEBI	-	builds acid from
[Ref.: #65552]	gentiobiose ChEBI	+/-	builds acid from
[Ref.: #65552]	gluconate ChEBI	-	builds acid from
[Ref.: #65552]	glucose ChEBI	-	builds gas from
[Ref.: #65552]	glucose ChEBI	+	fermentation
[Ref.: #65552]	glycerol ChEBI	-	builds acid from
[Ref.: #65552]	glycogen ChEBI	-	builds acid from
[Ref.: #65552]	inulin ChEBI	-	builds acid from
[Ref.: #65552]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65552]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65552]	L-fucose ChEBI	-	builds acid from
[Ref.: #65552]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65552]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65552]	L-xylose ChEBI	-	builds acid from
[Ref.: #65552]	lactose ChEBI	-	builds acid from
[Ref.: #65552]	maltose ChEBI	-	builds acid from
[Ref.: #65552]	melezitose ChEBI	-	builds acid from
[Ref.: #65552]	melibiose ChEBI	-	builds acid from
[Ref.: #65552]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65552]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65552]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65552]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65552]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65552]	nitrate ChEBI	-	reduction
[Ref.: #65552]	raffinose ChEBI	-	builds acid from
[Ref.: #65552]	ribitol ChEBI	-	builds acid from
[Ref.: #65552]	salicin ChEBI	+/-	builds acid from
[Ref.: #65552]	starch ChEBI	-	builds acid from
[Ref.: #65552]	sucrose ChEBI	-	builds acid from
[Ref.: #65552]	trehalose ChEBI	+/-	builds acid from
[Ref.: #65552]	turanose ChEBI	-	builds acid from
[Ref.: #65552]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65552]	alanine arylamidase	+	3.4.11.2
[Ref.: #65552]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65552]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65552]	catalase	-	1.11.1.6
[Ref.: #65552]	glycin arylamidase	+
[Ref.: #65552]	phenylalanine arylamidase	+
[Ref.: #65552]	proline-arylamidase	+	3.4.11.5
[Ref.: #65552]	tyrosine arylamidase	+

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65552]

Fatty acid	Percentage
C18:1ω9c	52.1
C16:0	17.8
unknown 18.846 / C19:1ω6c / C19:0 cycl	11.9
C18:1ω6c / C18:1ω7c	10.3
C18:0	3.8
C19:0 iso	2.3
C16:1ω6c / C16:1ω7c	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65552]	Sample type/isolated from	gut of grasshopper Metrioptera engelhardti
[Ref.: #65552]	Sampling date	2015-09
[Ref.: #65552]	Geographic location (country and/or sea, region)	Nonomi plateau, Nagano Prefecture
[Ref.: #65552]	Country	Japan
[Ref.: #65552]	Country ISO 3 Code	JPN
[Ref.: #65552]	Continent	Asia
[Ref.: #65552]	Geographic location	37.0000°/138.5670°
[Ref.: #65552]	Enrichment culture	MRS agar

[Ref.: #67770]	Sample type/isolated from	Grasshopper, Metrioptera engelhardti (Japanese common name: himegisu)
[Ref.: #67770]	Host species	Metrioptera engelhardti
[Ref.: #67770]	Geographic location (country and/or sea, region)	mountainous areas of the Nonomi plateau in Nagano Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65552]	16S rRNA gene sequence	LC190736		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Companilactobacillus metriopterae JCM 31635	GCA_004117915	contig	GenBank	1909267 tax ID	*
[Ref.: #66792]	Lactobacillus metriopterae strain JCM 31635	1909267.3	wgs	PATRIC	1909267 tax ID	*
[Ref.: #66792]	Companilactobacillus metriopterae JCM 31635	2881902429	draft	JGI IMG/M	1909267 tax ID	*
[Ref.: #67770]	Companilactobacillus metriopterae strain JCM 31635, whole genome shotgun sequencing project	RSTD00000000		GenBank	1909267 tax ID

[Ref.: #65552]	GC-content	37.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	32.8 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	37-37.6 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #67232]

Culture collection no.

JCM 31635, DSM 103730, Hime 5-1

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti.	Chiba M, Itabashi T, Hirai K, Sakamoto M, Ohkuma M, Ishige T, Kawasaki S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002694	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65552

Makoto Chiba, Takanori Itabashi, Koujiro Hirai, Mitsuo Sakamoto, Moriya Ohkuma, Taichiro Ishige and Shinji Kawasaki: Lactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti. IJSEM 68: 1484 - 1489 2018 ( DOI 10.1099/ijsem.0.002694 , PubMed 29543150 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67232

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103730

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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