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Strain identifier

BacDive ID: 158793

Type strain: Yes

Species: Companilactobacillus metriopterae

Strain Designation: Hime5-1

Strain history: S. Kawasaki; Dept. of Biosci., Tokyo Univ. of Agric., Japan; Hime5-1.

NCBI tax ID(s): 1909267 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
version 7 (current version):
version 6:
version 5:
version 4.1:
version 7 (current version)


@ref: 67232

BacDive-ID: 158793

DSM-Number: 103730

keywords: facultative anaerobe, Bacteria, 16S sequence, genome sequence, Gram-positive, rod-shaped

description: Companilactobacillus metriopterae Hime5-1 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from gut of grasshopper Metrioptera engelhardti.

NCBI tax id

  • NCBI tax id: 1909267
  • Matching level: species

strain history

doi: 10.13145/bacdive158793.20220920.7

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Companilactobacillus
  • species: Companilactobacillus metriopterae
  • full scientific name: Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020
  • synonyms

    20215Lactobacillus metriopterae
    20215Lactobacillus terrae

@ref: 67232

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Companilactobacillus

species: Companilactobacillus metriopterae

full scientific name: Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020

strain designation: Hime5-1

type strain: yes


cell morphology

  • @ref: 65552
  • gram stain: positive
  • cell length: 0.6-1.2 µm
  • cell width: 0.2 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65552
  • colony size: 1-2 mm
  • colony color: yellowish
  • incubation period: 2 days
  • medium used: MRS agar

Culture and growth conditions

culture medium

65552MRS agaryes
67232MRS MEDIUM (DSMZ Medium 11)yeshttps://bacmedia.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp


Physiology and metabolism

oxygen tolerance

  • @ref: 65552
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 65552
  • spore formation: no


@refsaltgrowthtested relationconcentration
65552NaClpositivegrowth7.0 %(w/v)
65552NaClnogrowth7.5 %(w/v)


65552singly, in pairs or short chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65552168082-dehydro-D-gluconate-builds acid from
65552581435-dehydro-D-gluconate-builds acid from
6555227613amygdalin+/-builds acid from
6555218305arbutin-builds acid from
6555217057cellobiose+/-builds acid from
6555215963ribitol-builds acid from
6555217108D-arabinose-builds acid from
6555218333D-arabitol-builds acid from
6555215824D-fructose+builds acid from
6555228847D-fucose-builds acid from
6555212936D-galactose+/-builds acid from
6555217634D-glucose+builds acid from
6555262318D-lyxose-builds acid from
6555216899D-mannitol-builds acid from
6555216024D-mannose+builds acid from
6555216988D-ribose+builds acid from
6555217924D-sorbitol-builds acid from
6555216443D-tagatose-builds acid from
6555265327D-xylose-builds acid from
6555216813galactitol-builds acid from
6555217113erythritol-builds acid from
655524853esculin+builds acid from
6555228066gentiobiose+/-builds acid from
6555224265gluconate-builds acid from
6555217234glucose-builds gas from
6555217754glycerol-builds acid from
6555228087glycogen-builds acid from
6555217268myo-inositol-builds acid from
6555215443inulin-builds acid from
6555230849L-arabinose-builds acid from
6555218403L-arabitol-builds acid from
6555218287L-fucose-builds acid from
6555262345L-rhamnose-builds acid from
6555217266L-sorbose-builds acid from
6555265328L-xylose-builds acid from
6555217716lactose-builds acid from
6555217306maltose-builds acid from
655526731melezitose-builds acid from
6555228053melibiose-builds acid from
65552320061methyl alpha-D-glucopyranoside-builds acid from
6555243943methyl alpha-D-mannoside-builds acid from
6555274863methyl beta-D-xylopyranoside-builds acid from
65552506227N-acetylglucosamine+builds acid from
6555216634raffinose-builds acid from
6555217814salicin+/-builds acid from
6555228017starch-builds acid from
6555217992sucrose-builds acid from
6555227082trehalose+/-builds acid from
6555232528turanose-builds acid from
6555217151xylitol-builds acid from


65552arginine dihydrolase-
65552alkaline phosphatase+
65552phenylalanine arylamidase+
65552tyrosine arylamidase+
65552alanine arylamidase+
65552glycin arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65552C19:0 iso2.3
    65552C16:1ω6c / C16:1ω7c1
    65552unknown 18.846 / C19:1ω6c / C19:0 cycl11.9
    65552C18:1ω6c / C18:1ω7c10.3
  • type of FA analysis: whole cell analysis
  • incubation medium: MRS
  • incubation temperature: 37
  • incubation time: 2
  • incubation_oxygen: anaerobic
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information


@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment culturehost species
65552gut of grasshopper Metrioptera engelhardti2015-09Nonomi plateau, Nagano PrefectureJapanJPNAsia37138.567MRS agar
67770Grasshopper, Metrioptera engelhardti (Japanese common name: himegisu)mountainous areas of the Nonomi plateau in Nagano Pref.JapanJPNAsiaMetrioptera engelhardti

isolation source categories

#Host Body-Site#Gastrointestinal tract

Sequence information

16S sequences

  • @ref: 65552
  • description: 16S rRNA gene sequence
  • accession: LC190736
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Companilactobacillus metriopterae JCM 31635GCA_004117915contigncbi1909267
66792Lactobacillus metriopterae strain JCM 316351909267.3wgspatric1909267
66792Companilactobacillus metriopterae JCM 316352881902429draftimg1909267
67770Companilactobacillus metriopterae strain JCM 31635, whole genome shotgun sequencing projectRSTD00000000ncbi1909267

GC content

6555237.3high performance liquid chromatography (HPLC)
6777032.8genome sequence analysis
6777037-37.6high performance liquid chromatography (HPLC)

External links

@ref: 67232

culture collection no.: JCM 31635, DSM 103730, Hime 5-1


  • topic: Phylogeny
  • Pubmed-ID: 29543150
  • title: Lactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti.
  • authors: Chiba M, Itabashi T, Hirai K, Sakamoto M, Ohkuma M, Ishige T, Kawasaki S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002694
  • year: 2018
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Grasshoppers/*microbiology, Japan, Lactobacillus/*classification/genetics/isolation & purification, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome


20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65552Makoto Chiba, Takanori Itabashi, Koujiro Hirai, Mitsuo Sakamoto, Moriya Ohkuma, Taichiro Ishige and Shinji KawasakiLactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti10.1099/ijsem.0.002694IJSEM 68: 1484-1489 201829543150
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67232Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103730Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103730)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/