Strain identifier

BacDive ID: 158793

Type strain: Yes

Species: Companilactobacillus metriopterae

Strain Designation: Hime5-1

Strain history: S. Kawasaki; Dept. of Biosci., Tokyo Univ. of Agric., Japan; Hime5-1.

NCBI tax ID(s): 1909267 (species)

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General

@ref: 67232

BacDive-ID: 158793

DSM-Number: 103730

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, rod-shaped

description: Companilactobacillus metriopterae Hime5-1 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from gut of grasshopper Metrioptera engelhardti.

NCBI tax id

  • NCBI tax id: 1909267
  • Matching level: species

strain history

@refhistory
67232<- S. Kawasaki, Dept. Bio-Science, Tokyo Univ. Agriculture, Japan; Hime5-1 <- S. Kawasaki and M. Chiba
67770S. Kawasaki; Dept. of Biosci., Tokyo Univ. of Agric., Japan; Hime5-1.

doi: 10.13145/bacdive158793.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Companilactobacillus
  • species: Companilactobacillus metriopterae
  • full scientific name: Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus metriopterae
    20215Lactobacillus terrae

@ref: 67232

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Companilactobacillus

species: Companilactobacillus metriopterae

full scientific name: Companilactobacillus metriopterae (Chiba et al. 2018) Zheng et al. 2020

strain designation: Hime5-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
65552positive0.6-1.2 µm0.2 µmrod-shapedno
69480no97.899
69480positive100

colony morphology

  • @ref: 65552
  • colony size: 1-2 mm
  • colony color: yellowish
  • incubation period: 2 days
  • medium used: MRS agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65552MRS agaryes
67232MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
65552positivegrowth15-37
65552nogrowth10psychrophilic
65552nogrowth45thermophilic
67232positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 65552
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
65552no
69480no99.999
69481no100

halophily

@refsaltgrowthtested relationconcentration
65552NaClpositivegrowth7.0 %(w/v)
65552NaClnogrowth7.5 %(w/v)

observation

@refobservation
65552singly, in pairs or short chains
65552homofermentative

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65552168082-dehydro-D-gluconate-builds acid from
65552581435-dehydro-D-gluconate-builds acid from
6555227613amygdalin+/-builds acid from
6555218305arbutin-builds acid from
6555217057cellobiose+/-builds acid from
6555215963ribitol-builds acid from
6555217108D-arabinose-builds acid from
6555218333D-arabitol-builds acid from
6555215824D-fructose+builds acid from
6555228847D-fucose-builds acid from
6555212936D-galactose+/-builds acid from
6555217634D-glucose+builds acid from
6555262318D-lyxose-builds acid from
6555216899D-mannitol-builds acid from
6555216024D-mannose+builds acid from
6555216988D-ribose+builds acid from
6555217924D-sorbitol-builds acid from
6555216443D-tagatose-builds acid from
6555265327D-xylose-builds acid from
6555216813galactitol-builds acid from
6555217113erythritol-builds acid from
655524853esculin+builds acid from
6555228066gentiobiose+/-builds acid from
6555224265gluconate-builds acid from
6555217234glucose-builds gas from
6555217234glucose+fermentation
6555217754glycerol-builds acid from
6555228087glycogen-builds acid from
6555217268myo-inositol-builds acid from
6555215443inulin-builds acid from
6555230849L-arabinose-builds acid from
6555218403L-arabitol-builds acid from
6555218287L-fucose-builds acid from
6555262345L-rhamnose-builds acid from
6555217266L-sorbose-builds acid from
6555265328L-xylose-builds acid from
6555217716lactose-builds acid from
6555217306maltose-builds acid from
655526731melezitose-builds acid from
6555228053melibiose-builds acid from
65552320061methyl alpha-D-glucopyranoside-builds acid from
6555243943methyl alpha-D-mannoside-builds acid from
6555274863methyl beta-D-xylopyranoside-builds acid from
65552506227N-acetylglucosamine+builds acid from
6555217632nitrate-reduction
6555216634raffinose-builds acid from
6555217814salicin+/-builds acid from
6555228017starch-builds acid from
6555217992sucrose-builds acid from
6555227082trehalose+/-builds acid from
6555232528turanose-builds acid from
6555217151xylitol-builds acid from

enzymes

@refvalueactivityec
65552catalase-1.11.1.6
65552arginine dihydrolase-3.5.3.6
65552alkaline phosphatase+3.1.3.1
65552proline-arylamidase+3.4.11.5
65552phenylalanine arylamidase+
65552tyrosine arylamidase+
65552alanine arylamidase+3.4.11.2
65552glycin arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65552C16:017.8
    65552C18:03.8
    65552C19:0 iso2.3
    65552C18:1ω9c52.1
    65552C16:1ω6c / C16:1ω7c1
    65552unknown 18.846 / C19:1ω6c / C19:0 cycl11.9
    65552C18:1ω6c / C18:1ω7c10.3
  • type of FA analysis: whole cell analysis
  • incubation medium: MRS
  • incubation temperature: 37
  • incubation time: 2
  • incubation_oxygen: anaerobic
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment culturehost species
65552gut of grasshopper Metrioptera engelhardti2015-09Nonomi plateau, Nagano PrefectureJapanJPNAsia37138.567MRS agar
67770Grasshopper, Metrioptera engelhardti (Japanese common name: himegisu)mountainous areas of the Nonomi plateau in Nagano Pref.JapanJPNAsiaMetrioptera engelhardti

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_96087.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1756;97_50083;98_65901;99_96087&stattab=map
  • Last taxonomy: Companilactobacillus
  • 16S sequence: LC190736
  • Sequence Identity:
  • Total samples: 137
  • soil counts: 24
  • aquatic counts: 1
  • animal counts: 105
  • plant counts: 7

Sequence information

16S sequences

  • @ref: 65552
  • description: 16S rRNA gene sequence
  • accession: LC190736
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus metriopterae strain JCM 316351909267.3wgspatric1909267
66792Companilactobacillus metriopterae JCM 316352881902429draftimg1909267
67770Companilactobacillus metriopterae JCM 31635GCA_004117915contigncbi1909267

GC content

@refGC-contentmethod
6555237.3high performance liquid chromatography (HPLC)
6777032.8genome sequence analysis
6777037-37.6high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.263yes
gram-positiveyes97.184yes
anaerobicno96.062no
aerobicno94.334no
halophileyes88.289no
spore-formingno96.327yes
glucose-utilyes92.444no
flagellatedno98.952no
thermophileno99.381no
glucose-fermentyes86.646yes

External links

@ref: 67232

culture collection no.: JCM 31635, DSM 103730, Hime 5-1

straininfo link

  • @ref: 111341
  • straininfo: 397160

literature

  • topic: Phylogeny
  • Pubmed-ID: 29543150
  • title: Lactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti.
  • authors: Chiba M, Itabashi T, Hirai K, Sakamoto M, Ohkuma M, Ishige T, Kawasaki S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002694
  • year: 2018
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Grasshoppers/*microbiology, Japan, Lactobacillus/*classification/genetics/isolation & purification, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65552Makoto Chiba, Takanori Itabashi, Koujiro Hirai, Mitsuo Sakamoto, Moriya Ohkuma, Taichiro Ishige and Shinji KawasakiLactobacillus metriopterae sp. nov., a novel lactic acid bacterium isolated from the gut of grasshopper Metrioptera engelhardti10.1099/ijsem.0.002694IJSEM 68: 1484-1489 201829543150
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67232Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103730Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103730)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111341Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397160.1