Name: Lysobacter olei D-14
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158751

Type strain:

Species: Lysobacter olei

Strain Designation: D-14

Culture col. no.: KEMB 9005-572, KACC 19173, JCM 31917

Strain history: J. Kim; Kyonggi Univ., South Korea; D-14.

NCBI tax ID(s): 1926870 (species)

Special Collections

Section

Lysobacter olei D-14 is an aerobe, non-halophilic, Gram-negative bacterium that forms circular colonies and was isolated from oil-contaminated soil.

colony-forming
Gram-negative
rod-shaped
aerobe
non-halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Lysobacterales*
	Family	*Lysobacteraceae*
	Genus	*Lysobacter*
	Species	**Lysobacter olei**
	Full Scientific Name (LPSN)	Lysobacter olei Chaudhary et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65503]	Gram stain	negative

[Ref.: #65503]	Cell length	1.2-1.5 µm

[Ref.: #65503]	Cell width	0.4-0.6 µm

[Ref.: #65503]	Cell shape	rod-shaped

[Ref.: #65503]	Motility	no

[Ref.: #65503]	Colony size	0.5-1.0 mm
[Ref.: #65503]	Colony color	brown
[Ref.: #65503]	Colony shape	circular
[Ref.: #65503]	Incubation period	5 days
[Ref.: #65503]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Trypticase Soy Agar (TSA)

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

MacConkey

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

BHI

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Culture medium growth

		Temperature
[Ref.: #65503]	growth	20-40 °C
[Ref.: #65503]	optimum	25-35 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #65503]			optimum	6.5-8.0
[Ref.: #65503]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

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Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65503]		NaCl		optimum	0	%
[Ref.: #65503]		NaCl		growth	0-1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65503]	2-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #65503]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65503]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65503]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65503]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #65503]	adipate ChEBI	-	assimilation
[Ref.: #65503]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65503]	casein	+	hydrolysis
[Ref.: #65503]	chitin ChEBI	-	hydrolysis
[Ref.: #65503]	D-glucose ChEBI	-	assimilation
[Ref.: #65503]	D-glucose ChEBI	+	assimilation
[Ref.: #65503]	D-mannitol ChEBI	-	assimilation
[Ref.: #65503]	D-mannose ChEBI	-	assimilation
[Ref.: #65503]	D-ribose ChEBI	-	assimilation
[Ref.: #65503]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65503]	decanoate ChEBI	-	assimilation
[Ref.: #65503]	dna ChEBI	+	hydrolysis
[Ref.: #65503]	esculin ChEBI	-	hydrolysis
[Ref.: #65503]	esculin ferric citrate	-	hydrolysis
[Ref.: #65503]	gelatin ChEBI	+	hydrolysis
[Ref.: #65503]	glucose ChEBI	-	fermentation
[Ref.: #65503]	glycogen ChEBI	-	assimilation
[Ref.: #65503]	itaconate ChEBI	-	assimilation
[Ref.: #65503]	L-alanine ChEBI	-	assimilation
[Ref.: #65503]	L-arabinose ChEBI	-	assimilation
[Ref.: #65503]	L-fucose ChEBI	-	assimilation
[Ref.: #65503]	L-histidine ChEBI	-	assimilation
[Ref.: #65503]	L-proline ChEBI	-	assimilation
[Ref.: #65503]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65503]	L-serine ChEBI	-	assimilation
[Ref.: #65503]	lactate ChEBI	-	assimilation
[Ref.: #65503]	malate ChEBI	-	assimilation
[Ref.: #65503]	maltose ChEBI	+	assimilation
[Ref.: #65503]	maltose ChEBI	+/-	assimilation
[Ref.: #65503]	melibiose ChEBI	-	assimilation
[Ref.: #65503]	myo-inositol ChEBI	-	assimilation
[Ref.: #65503]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65503]	nitrate ChEBI	-	reduction
[Ref.: #65503]	phenylacetate ChEBI	-	assimilation
[Ref.: #65503]	potassium gluconate ChEBI	-	assimilation
[Ref.: #65503]	propionate ChEBI	-	assimilation
[Ref.: #65503]	salicin ChEBI	-	assimilation
[Ref.: #65503]	sodium acetate ChEBI	-	assimilation
[Ref.: #65503]	sodium citrate ChEBI	-	assimilation
[Ref.: #65503]	sodium malonate ChEBI	-	assimilation
[Ref.: #65503]	starch ChEBI	-	hydrolysis
[Ref.: #65503]	suberic acid ChEBI	-	assimilation
[Ref.: #65503]	sucrose ChEBI	+/-	assimilation
[Ref.: #65503]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65503]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65503]	tyrosine ChEBI	-	hydrolysis
[Ref.: #65503]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65503]	acetoin ChEBI	no
[Ref.: #65503]	hydrogen sulfide ChEBI	no
[Ref.: #65503]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #65503]		acetoin ChEBI	-
[Ref.: #65503]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #65503]	acid phosphatase	+	3.1.3.2
[Ref.: #65503]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65503]	alpha-chymotrypsin	+/-	3.4.21.1
[Ref.: #65503]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65503]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65503]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65503]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65503]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65503]	beta-galactosidase	+/-	3.2.1.23
[Ref.: #65503]	beta-glucosidase	-	3.2.1.21
[Ref.: #65503]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65503]	catalase	+	1.11.1.6
[Ref.: #65503]	cystine arylamidase	+/-	3.4.11.3
[Ref.: #65503]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65503]	esterase (C 4)	+/-
[Ref.: #65503]	esterase Lipase (C 8)	+/-
[Ref.: #65503]	leucine arylamidase	+	3.4.11.1
[Ref.: #65503]	lipase (C 14)	-
[Ref.: #65503]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65503]	naphthol-AS-BI-phosphohydrolase	+/-
[Ref.: #65503]	trypsin	+	3.4.21.4
[Ref.: #65503]	urease	-	3.5.1.5
[Ref.: #65503]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65503]

Fatty acid	Percentage
C15:0 iso	22.2
C16:0 iso	20.1
C17:1ω9c / C16:0 10-methyl	13.2
C16:1ω7c / C16:1ω6c	8.8
C14:0 iso	8.3
C11:0 iso 3OH	6.2
C15:1 iso F	6.1
C16:0	5.5
C11:0 iso	4.7
C14:0	1.7
C15:0 anteiso	1.2
C17:0 iso	1.2
C16:1 iso H	0.8
C12:0 iso	0.8
C17:0 cyclo	0.6
C13:0 iso	0.6
C10:0 iso	0.5
C12:0 iso 3OH	0.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65503]	Sample type/isolated from	oil-contaminated soil
[Ref.: #65503]	Geographic location (country and/or sea, region)	Gunsan, North Jeolla Province
[Ref.: #65503]	Country	Republic of Korea
[Ref.: #65503]	Country ISO 3 Code	KOR
[Ref.: #65503]	Continent	Asia
[Ref.: #65503]	Enrichment culture duration	2 days
[Ref.: #65503]	Enrichment culture temperature	28 ̊C
[Ref.: #65503]	Isolation/enrichment procedure	modified culture technique with six-well polycarbonate transwell plates and streaked on R2A medium

[Ref.: #67770]	Sample type/isolated from	Oil-contaminated soil of Gunsan
[Ref.: #67770]	Geographic location (country and/or sea, region)	North Jeolla Province
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

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Global distribution of 16S sequence KY313413 (>99% sequence identity) for Lysobacter from Microbeatlas

Aquatic Samples	835
Soil Samples	3732
Animal Samples	239
Plant Samples	430
Total Samples	5236

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65503]	Lysobacter olei 16S ribosomal RNA gene, partial sequence	KY313413		GenBank ENA

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GC-content

68.7 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

KEMB 9005-572, KACC 19173, JCM 31917

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lysobacter olei sp. nov., isolated from oil-contaminated soil.	Chaudhary DK, Lee SD, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002348	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65503

Dhiraj Kumar Chaudhary, Sang Don Lee and Jaisoo Kim: Lysobacter olei sp. nov., isolated from oil-contaminated soil. IJSEM 67: 4660 - 4666 2017 ( DOI 10.1099/ijsem.0.002348 , PubMed 28984550 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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