Name: Stenotrophomonas lactitubi M15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158735

Type strain:

Species: Stenotrophomonas lactitubi

Strain Designation: M15

Culture col. no.: LMG 29943, DSM 104152, CIP 111740

Strain history: CIP <- 2019, LMG <- M. Weber, Friedrich Wilhelms Univ., Bonn, Germany: strain M15

NCBI tax ID(s): 2045214 (species)

SI-ID 399284

DSM 104152

Section

Stenotrophomonas lactitubi M15 is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from milking machine biofilms on a dairy farm during the characterization of the microbiota of milking machine biofilms on a dairy farm.

colony-forming
Gram-negative
rod-shaped
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Lysobacterales*
	Family	*Lysobacteraceae*
	Genus	*Stenotrophomonas*
	Species	**Stenotrophomonas lactitubi**
	Full Scientific Name (LPSN)	Stenotrophomonas lactitubi Weber et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65486]	Gram stain	negative
[Ref.: #120534]	Gram stain	negative

[Ref.: #65486]	Cell length	2.5-3.7 µm

[Ref.: #65486]	Cell width	0.9-1.1 µm

[Ref.: #65486]	Cell shape	rod-shaped
[Ref.: #120534]	Cell shape	rod-shaped

[Ref.: #120534]	Motility	yes

[Ref.: #65486]	Colony color	yellowish
[Ref.: #65486]	Colony shape	circular
[Ref.: #65486]	Cultivation medium used	Trypticase soy agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65486]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #65486]

Culture medium growth

[Ref.: #66319]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #66319]

Culture medium growth

[Ref.: #66319]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66319]

Culture medium composition

expand / minimize

[Ref.: #120534]

Culture medium

CIP Medium 72

[Ref.: #120534]

Culture medium growth

[Ref.: #120534]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #65486]		growth	6-37 °C
[Ref.: #65486]	no	growth	40 °C
[Ref.: #66319]		growth	28 °C

[Ref.: #65486]	Temperature range	thermophilic
[Ref.: #66319]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65486]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65486]

Oxygen tolerance

obligate aerobe

[Ref.: #65486]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65486]		NaCl		growth	3	%
[Ref.: #65486]		NaCl	no	growth	4	%

[Ref.: #65486]	Observation	Q8 is the predominant ubiquinone
[Ref.: #65486]	Observation	polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and traces of an amino phospholipid

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #65486]	cellobiose ChEBI	-	builds acid from
[Ref.: #65486]	D-fructose ChEBI	+/-	builds acid from
[Ref.: #65486]	D-glucose ChEBI	+/-	builds acid from
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #65486]	D-mannose ChEBI	+	builds acid from
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #65486]	D-xylose ChEBI	-	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #65486]	esculin ChEBI	+	assimilation
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #65486]	lactose ChEBI	-	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #65486]	melibiose ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #65486]	potassium 2-dehydro-D-gluconate	+	assimilation
[Ref.: #65486]	salicin ChEBI	-	assimilation
[Ref.: #65486]	sucrose ChEBI	-	assimilation
[Ref.: #65486]	sucrose ChEBI	-	builds acid from
[Ref.: #65486]	trehalose ChEBI	-	builds acid from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #65486]	turanose ChEBI	-	assimilation
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #65486]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #65486]	catalase	+	1.11.1.6
[Ref.: #65486]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65486]

Fatty acid	Percentage
C15:0 iso	47.7
C15:0 anteiso	25.8
C16:0	9.5
C16:1cis9	4.8
C11:0 iso	3.9
C14:0	2.2
C14:0 iso	1.7
C16:0 iso	1.7
C15:0	0.8
C17:0 iso	0.6
C13:0 iso 3OH	0.6
C12:0 3OH	0.4
C10:0	0.2
C11:0 iso 3OH	0.1

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #66319]	25466	+	-	-	-	-	+	+	+	+	-	+	-	+	+	-	-	-	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65486]	Sample type/isolated from	milking machine biofilms on a dairy farm (milking equipment retainer biofilms) during the characterization of the microbiota of milking machine biofilms on a dairy farm
[Ref.: #65486]	Geographic location (country and/or sea, region)	Königswinter
[Ref.: #65486]	Country	Germany
[Ref.: #65486]	Country ISO 3 Code	DEU
[Ref.: #65486]	Continent	Europe
[Ref.: #65486]	Geographic location	50.7138°/7.2069°
[Ref.: #65486]	Isolation/enrichment procedure	were picked from cetrimide agar (CFC), was isolated from trypticase soy agar (TSA) containing cloxacillin (2µg ml^-1)

[Ref.: #66319]	Sample type/isolated from	Milking machine biofilm
[Ref.: #66319]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #66319]	Country	Germany
[Ref.: #66319]	Country ISO 3 Code	DEU
[Ref.: #66319]	Continent	Europe
[Ref.: #66319]	Geographic location	50.7374°/7.0982°

[Ref.: #120534]	Sample type/isolated from	Milking machine, biofilm
[Ref.: #120534]	Geographic location (country and/or sea, region)	Königswinter Vinxel, Nord-Rhine-Westfalia, Germany
[Ref.: #120534]	Country	Germany
[Ref.: #120534]	Country ISO 3 Code	DEU
[Ref.: #120534]	Continent	Europe
[Ref.: #120534]	Isolation date	2014-03-19
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Engineered product
#Engineered	#Agriculture	#Livestock (Husbandry)
#Environmental	#Biofilm	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT222224 (>99% sequence identity) for Stenotrophomonas from Microbeatlas

Aquatic Samples	1647
Soil Samples	1650
Animal Samples	6387
Plant Samples	767
Total Samples	10451

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #120534]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65486]	16S rRNA gene sequence	LT222224		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Stenotrophomonas lactitubi M15	GCA_002803515	contig	GenBank	2045214 tax ID	*
[Ref.: #66792]	Stenotrophomonas lactitubi M15	2869333513	draft	JGI IMG/M	2045214 tax ID	*

Availability in culture collections External links

[Ref.: #66319]

Culture collection no.

LMG 29943, DSM 104152, CIP 111740

[Ref.: #111333]

SI-ID 399284

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Stenotrophomonas lactitubi sp. nov. and Stenotrophomonas indicatrix sp. nov., isolated from surfaces with food contact.	Weber M, Schunemann W, Fuss J, Kampfer P, Lipski A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002732	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65486

Mareike Weber, Wiebke Schünemann, Janina Fuß, Peter Kämpfer, André Lipski: Stenotrophomonas lactitubi sp. nov. and Stenotrophomonas indicatrix sp. nov., isolated from surfaces with food contact. IJSEM 68: 1830 - 1838 2018 ( DOI 10.1099/ijsem.0.002732 , PubMed 29638210 )

#66319

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104152

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#111333

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399284.1 )

#120534 Collection of Institut Pasteur ; Curators of the CIP; CIP 111740
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Stenotrophomonas lactitubi in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF