Simplicispira soli (KEMB 9005-529, KACC 19107, NBRC 112689)

Name: Simplicispira soli (KEMB 9005-529, KACC 19107, NBRC 112689)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158734

Type strain

Strain Designation CA-16

Culture col. no. KEMB 9005-529 KACC 19107 NBRC 112689 CA-16

NCBI tax ID(s) 2083185

Special Collections Literature strains

Links

Simplicispira soli CA-16 is an aerobe, Gram-negative, motile prokaryote that forms circular colonies and was isolated from a stream bank soil sample from near Kyonggi University.

Gram-negative motile rod-shaped colony-forming aerobe 16S sequence

Name and taxonomic classification

SI-ID 132806

NBRC 112689

@ref 20215

Last LPSN update 2025-12-16 10:08:05

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Comamonadaceae

Genus Simplicispira

Species Simplicispira soli

Full scientific name Simplicispira soli Dahal and Kim 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
65485	negative	1.5-2.1 µm	0.5-0.7 µm	rod-shaped		polar

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
65485	2-3 mm	brownish cream	circular	4 days	Tryptic Soy Agar

Pigmentation

@ref	Production	Name	Color
65485		flexirubin pigments
65485			red

Culture and growth conditions

Culture medium

@ref	Name	Growth
65485	Reasoner's 2A agar
65485	veal infusion agar
65485	Nutrient agar (NA)
65485	Mueller Hinton Agar
65485	Brain Heart Infusion
65485	Tryptic Soy Agar
65485	Marine agar (MA)
65485	MacConkey agar
65485	Laked Blood Agar

Culture temp

@ref	Growth	Type	Temperature (°C)
65485	positive	growth	15-37
65485	positive	optimum	20-32

Culture pH

@ref	Ability	Type	PH	PH range
65485	positive	optimum	6.5-9.0
65485	positive	growth	5.5-10.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

65485

Oxygen toleranceaerobe

Spore formation

65485

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65485	NaCl	positive	optimum	0 %
65485	NaCl	positive	growth	0-1.5 %

Observation

@ref	Observation
65485	major isoprenoid quinone is Q-8
65485	major polar lipids are phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65485		casein	-	hydrolysis
65485	62968 ChEBI	cellulose	-	hydrolysis
65485	17029 ChEBI	chitin	-	hydrolysis
65485	16991 ChEBI	dna	-	hydrolysis
65485	17234 ChEBI	glucose	-	fermentation
65485	17368 ChEBI	hypoxanthine	-	hydrolysis
65485	16977 ChEBI	L-alanine	+	assimilation
65485	15971 ChEBI	L-histidine	+	assimilation
65485	17203 ChEBI	L-proline	+	assimilation
65485	24996 ChEBI	lactate	+	assimilation
65485	25115 ChEBI	malate	+	assimilation
65485	17632 ChEBI	nitrate	-	reduction
65485	32954 ChEBI	sodium acetate	+	assimilation
65485	28017 ChEBI	starch	-	hydrolysis
65485	53423 ChEBI	tween 40	+	hydrolysis
65485	53425 ChEBI	tween 60	+	hydrolysis
65485	53426 ChEBI	tween 80	-	hydrolysis
65485	18186 ChEBI	tyrosine	+	hydrolysis
65485	31011 ChEBI	valerate	+	assimilation

Enzymes

@ref	Value	Activity	Ec
65485	acid phosphatase	-	3.1.3.2
65485	alkaline phosphatase	+	3.1.3.1
65485	alpha-chymotrypsin	+	3.4.21.1
65485	alpha-fucosidase	-	3.2.1.51
65485	alpha-galactosidase	-	3.2.1.22
65485	alpha-glucosidase	-	3.2.1.20
65485	alpha-mannosidase	-	3.2.1.24
65485	beta-galactosidase	-	3.2.1.23
65485	beta-glucosidase	-	3.2.1.21
65485	beta-glucuronidase	-	3.2.1.31
65485	catalase	-	1.11.1.6
65485	cystine arylamidase	+	3.4.11.3
65485	cytochrome oxidase	+	1.9.3.1
65485	esterase (C 4)	+
65485	esterase Lipase (C 8)	+
65485	leucine arylamidase	+	3.4.11.1
65485	lipase (C 14)	-
65485	N-acetyl-beta-glucosaminidase	-	3.2.1.52
65485	naphthol-AS-BI-phosphohydrolase	-
65485	trypsin	-	3.4.21.4
65485	urease	-	3.5.1.5
65485	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

65485

Fatty acid	Percentage
C16:1ω7c and/or C16:1ω6c	46.2
C16:0	26.4
C18:1ω7c and/or C18:1ω6c	8.5
C18:1ω7c-11methyl	6.5
C12:0	6
C10:0 3OH	3
C14:0	1.2
C8:0 3OH	1.2
C17:0	0.4
C18:0	0.3
C10:0	0.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#River (Creek)
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation procedure
65485	a stream bank soil sample from near Kyonggi University	Suwon, Gyeonggi-Do	Republic of Korea	KOR	Asia	Bacterial strains were isolated using a modified culture technique with six-well polycarbonate Transwell plates (Corning). Isolation, routine culture and preservation were done as described previously

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
65485	Simplicispira soli strain CA-16 16S ribosomal RNA gene, partial sequence	KY031325

GC content

@ref	GC-content (mol%)	Method
65485	63.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Simplicispira soli sp. nov., a betaproteobacterium isolated from stream bank soil.	Dahal RH, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002618	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65485	Ram Hari Dahal, Jaisoo Kim: Simplicispira soli sp. nov., a betaproteobacterium isolated from stream bank soil. IJSEM 68: 951 - 956 2018 ( DOI 10.1099/ijsem.0.002618 , PubMed 29458495 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Simplicispira soli (KEMB 9005-529, KACC 19107, NBRC 112689)

Name and taxonomic classification