Name: Saccharolobus caldissimus HS-3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158656

Type strain:

Species: Saccharolobus caldissimus

Strain Designation: HS-3

Culture col. no.: JCM 32116, InaCC Ar80

Strain history: N. Kurosawa; Fac. of Sci. & Eng., Soka Univ., Japan; HS-3.

NCBI tax ID(s): 1702097 (species)

Section

Saccharolobus caldissimus HS-3 is a hyperthermophilic, coccus-shaped archaeon that was isolated from acidic terrestrial hot spring.

coccus-shaped
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Sulfolobales*
	Family	*Sulfolobaceae*
	Genus	*Saccharolobus*
	Species	**Saccharolobus caldissimus**
	Full Scientific Name (LPSN)	Saccharolobus caldissimus Sakai and Kurosawa 2018

Information on morphological and physiological properties Morphology

[Ref.: #65383]	Cell length	0.8-2.2 µm

[Ref.: #65383]	Cell shape	coccus-shaped

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #65383]	growth	65-93 °C
[Ref.: #65383]	optimum	85 °C
[Ref.: #67770]	growth	85 °C

[Ref.: #65383]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic

	pH	Kind of pH		pH
[Ref.: #65383]			optimum	3
[Ref.: #65383]			growth	1.5-6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65383]	Observation	Colony forming efficiency is quite low (less than 0.001 %).
[Ref.: #65383]	Observation	Heterotrophic growth occurs on following complex substrates (yeast extract, peptone, tryptone, casamino acids) and sugars (starch, sucrose, lactose, maltose, raffinose, D-glucose, D-galactose, D-mannose, D-arabinose and L-arabinose).
[Ref.: #65383]	Observation	Chemolithotrophic growth occurs on pyrite (FeS 2 ) but not on elemental sulfur, K2S4O6 , and oxidation of hydrogen.
[Ref.: #65383]	Observation	Anaerobic growth on FeCl 3 occurs in the presence of yeast extract.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65383]	Sample type/isolated from	acidic terrestrial hot spring
[Ref.: #65383]	Geographic location (country and/or sea, region)	Ohwakudani, Hakone
[Ref.: #65383]	Country	Japan
[Ref.: #65383]	Country ISO 3 Code	JPN
[Ref.: #65383]	Continent	Asia
[Ref.: #65383]	Geographic location	35.2394°/139.0190°
[Ref.: #65383]	Enrichment culture	yeast extract 0.01 % (w/v); and a basal medium of modified Brock's basal salt (MBS)
[Ref.: #65383]	Enrichment culture composition	supplemented with 1.0 g yeast extract l-1, pH 6.0
[Ref.: #65383]	Enrichment culture temperature	83 ̊C
[Ref.: #65383]	Isolation/enrichment procedure	ENFE strategy (exploring novel micro-organisms in frozen enrichment cultures)

[Ref.: #67770]	Sample type/isolated from	Acidic terrestrial hot spring in Hakone Ohwaku-dani
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Acidic	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65383]	16S rRNA gene sequence	LC275065		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Saccharolobus caldissimus JCM 32116	GCA_020886315	complete	GenBank	1702097 tax ID	*
[Ref.: #66792]	Saccharolobus caldissimus strain JCM32116 strain JCM 32116	1702097.3	complete	PATRIC	1702097 tax ID	*

[Ref.: #65383]

GC-content

31.7 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Saccharolobus caldissimus JCM32116 NCBI: GCA_020886315.1
[Ref.: #69480]	flagellated	no	96.469	no
[Ref.: #69480]	gram-positive	no	92.349	no
[Ref.: #69480]	anaerobic	yes	70.03	no
[Ref.: #69480]	aerobic	no	74.727	no
[Ref.: #69480]	halophile	no	85.487	no
[Ref.: #69480]	spore-forming	no	93.832	no
[Ref.: #69480]	motile	no	81.326	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	83.544	no
[Ref.: #69480]	glucose-ferment	no	81.782	no

Availability in culture collections External links

[Ref.: #65383]

Culture collection no.

JCM 32116, InaCC Ar80

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of the Hyperthermophilic and Acidophilic Archaeon Saccharolobus caldissimus Strain HS-3(T).	Sakai HD, Kurosawa N	Microbiol Resour Announc	10.1128/mra.01078-21	2022	*
Phylogeny	Saccharolobus caldissimus gen. nov., sp. nov., a facultatively anaerobic iron-reducing hyperthermophilic archaeon isolated from an acidic terrestrial hot spring, and reclassification of Sulfolobus solfataricus as Saccharolobus solfataricus comb. nov. and Sulfolobus shibatae as Saccharolobus shibatae comb. nov.	Sakai HD, Kurosawa N	Int J Syst Evol Microbiol	10.1099/ijsem.0.002665	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65383

Hiroyuki D. Sakai, Norio Kurosawa: Saccharolobus caldissimus gen. nov., sp. nov., a facultatively anaerobic iron-reducing hyperthermophilic archaeon isolated from an acidic terrestrial hot spring, and reclassification of Sulfolobus solfataricus as Saccharolobus solfataricus comb. nov. and Sulfolobus shibatae as Saccharolobus shibatae comb. nov.. IJSEM 68: 1271 - 1278 2018 ( DOI 10.1099/ijsem.0.002665 , PubMed 29485400 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Saccharolobus caldissimus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Saccharolobus caldissimus JCM32116

Change proposal

Change proposal