Name: Malaciobacter canalis F138-33
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158655

Type strain:

Species: Malaciobacter canalis

Strain Designation: F138-33

Culture col. no.: CECT 8984, LMG 29148, CIP 111615

Strain history: CIP <- 2018, MJ. Figueiras, Rovira i Virgil Univ., Reus, Spain: strain F138-33

NCBI tax ID(s): 1912871 (species)

Section

Malaciobacter canalis F138-33 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from oysters exposed for 72 h to untreated urban sewage.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Arcobacteraceae*
	Genus	Malaciobacter
	Species	Malaciobacter canalis
	Full Scientific Name (LPSN)	Malaciobacter canalis corrig. (Pérez-Cataluña et al. 2018) Pérez-Cataluña et al. 2019

	Synonym	Malacobacter canalis
	Synonym	**Arcobacter canalis**

Information on morphological and physiological properties Morphology

[Ref.: #65382]	Gram stain	negative
[Ref.: #117127]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #65382]	Cell length	1.2-2.8 µm

[Ref.: #65382]	Cell width	0.2-0.4 µm

[Ref.: #65382]	Cell shape	rod-shaped
[Ref.: #117127]	Cell shape	rod-shaped

[Ref.: #65382]	Motility	yes
[Ref.: #117127]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.827

[Ref.: #65382]	Flagellum arrangement	monotrichous, polar

[Ref.: #65382]	Colony size	2-4 mm
[Ref.: #65382]	Colony color	pale yellow to pale orange
[Ref.: #65382]	Colony shape	circular
[Ref.: #65382]	Incubation period	2 days
[Ref.: #65382]	Cultivation medium used	marine agar 2216

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

minimal medium

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Culture medium composition

expand / minimize

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Culture medium

nutrient medium

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

0.005 % basic fuchsine

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Culture medium

0.001 % brilliant green

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Culture medium

MacConkey agar

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Culture medium composition

expand / minimize

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Culture medium

CIP Medium 13

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

blood agar

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Culture medium growth

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Culture medium

campylobacter charcoal deoxycholate agar

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Culture medium growth

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Culture medium

nutrient medium

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #65382]	growth	22-25 °C
[Ref.: #65382]	growth	30 °C
[Ref.: #65382]	growth	37 °C
[Ref.: #65382]	growth	42 °C

[Ref.: #65382]	Temperature range	mesophilic
[Ref.: #65382]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65382]	Oxygen tolerance	aerobe
[Ref.: #65382]	Oxygen tolerance	microaerophile
[Ref.: #65382]	Oxygen tolerance	anaerobe

[Ref.: #65382]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.938
[Ref.: #69481]	Ability of spore formation	no Confidence in %92

[Ref.: #65382]	Observation	Pigments are not produced in marine agar.
[Ref.: #65382]	Observation	After 48 h, colonies are smaller at 37 and 42°C than at room temperature or 30°C.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65382]	casein	-	hydrolysis
[Ref.: #65382]	glucose ChEBI	-	builds acid from
[Ref.: #65382]	indoxyl acetate	-	hydrolysis
[Ref.: #65382]	lecithin ChEBI	-	hydrolysis
[Ref.: #65382]	nitrate ChEBI	-	reduction
[Ref.: #65382]	starch ChEBI	-	hydrolysis
[Ref.: #65382]	urea ChEBI	-	hydrolysis

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65382]	cefalotin ChEBI	yes	0.03	µg/mL
[Ref.: #65382]	cefoperazone ChEBI	yes	64000	µg/mL
[Ref.: #65382]	nalidixic acid ChEBI	yes	0.03	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #65382]		hydrogen sulfide ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65382]		catalase	+	1.11.1.6
[Ref.: #65382]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65382]	Sample type/isolated from	oysters exposed for 72 h to untreated urban sewage
[Ref.: #65382]	Geographic location (country and/or sea, region)	Poble Nou canal, Catalonia
[Ref.: #65382]	Country	Spain
[Ref.: #65382]	Country ISO 3 Code	ESP
[Ref.: #65382]	Continent	Europe
[Ref.: #65382]	Geographic location	40.6418°/0.6936°
[Ref.: #65382]	Enrichment culture	Arcobacter CAT (cefoperazone, amphotericin B and teicoplanin) broth
[Ref.: #65382]	Enrichment culture composition	with 2.5% NaCl
[Ref.: #65382]	Enrichment culture temperature	30 ̊C
[Ref.: #65382]	Isolation/enrichment procedure	The isolation protocol involved an enrichment step in Arcobacter CAT (cefoperazone, amphotericin B and teicoplanin) broth supplemented with 2.5 % NaCl, followed by sub-culturing on marine agar at 30°C under aerobic and microaerobic conditions

[Ref.: #117127]	Sample type/isolated from	Environment, Urban sewage
[Ref.: #117127]	Geographic location (country and/or sea, region)	Sant Marti, Barcelona
[Ref.: #117127]	Country	Spain
[Ref.: #117127]	Country ISO 3 Code	ESP
[Ref.: #117127]	Continent	Europe
[Ref.: #117127]	Isolation date	2013
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Mollusca
#Engineered	#Waste	#Sewage sludge

What are isolation sources categories?

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Global distribution of 16S sequence MG015880 (>99% sequence identity) for Malaciobacter from Microbeatlas

Aquatic Samples	585
Soil Samples	47
Animal Samples	254
Plant Samples	9
Total Samples	895

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65382]	16S rRNA gene sequence	MG015880		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Malaciobacter canalis LMG 29148	GCA_008000835	complete	GenBank	1912871 tax ID	*
[Ref.: #66792]	Malaciobacter canalis F138-33	GCA_002723485	contig	GenBank	1912871 tax ID	*
[Ref.: #66792]	Arcobacter canalis strain LMG 29148	1912871.9	complete	PATRIC	1912871 tax ID	*
[Ref.: #66792]	Arcobacter canalis LMG 29148	2888026065	complete	JGI IMG/M	1912871 tax ID	*

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GC-content

27.5 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Malaciobacter canalis LMG 29148 NCBI: GCA_008000835.1
[Ref.: #69481]	spore-forming	no	92	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Malaciobacter canalis LMG 29148 NCBI: GCA_008000835.1
[Ref.: #69480]	motile	yes	88.23	no
[Ref.: #69480]	flagellated	yes	87.31	yes
[Ref.: #69480]	gram-positive	no	98.759	yes
[Ref.: #69480]	anaerobic	no	59.399	no
[Ref.: #69480]	aerobic	no	90.046	no
[Ref.: #69480]	halophile	no	68.371	no
[Ref.: #69480]	spore-forming	no	93.578	yes
[Ref.: #69480]	glucose-util	no	80.969	no
[Ref.: #69480]	thermophile	no	98.502	no
[Ref.: #69480]	glucose-ferment	no	86.904	no

Availability in culture collections External links

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Culture collection no.

CECT 8984, LMG 29148, CIP 111615

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65382

Alba Pérez-Cataluña, Nuria Salas-Massó, María José Figueras: Arcobacter canalis sp. nov., isolated from a water canal contaminated with urban sewage. IJSEM 68: 1258 - 1264 2018 ( DOI 10.1099/ijsem.0.002662 , PubMed 29488868 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#117127 Collection of Institut Pasteur ; Curators of the CIP; CIP 111615
* These data were automatically processed and therefore are not curated

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Malaciobacter canalis LMG 29148

Malaciobacter canalis LMG 29148

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