Name: Acinetobacter piscicola LW15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158638

Type strain:

Species: Acinetobacter piscicola

Strain Designation: LW15

Culture col. no.: MCCC 1K03337, CICC 24241, KCTC 62134, JCM 32101

Strain history: <- Yang Liu, Institute of Agricultural Quality Standards and Testing Technology Research

NCBI tax ID(s): 2006115 (species)

Section

Acinetobacter piscicola LW15 is a facultative anaerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from external lesions of diseased farmed Murray cod.

colony-forming
Gram-negative
rod-shaped
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Acinetobacter*
	Species	**Acinetobacter piscicola**
	Full Scientific Name (LPSN)	Acinetobacter piscicola Liu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65363]	Gram stain	negative

[Ref.: #65363]	Cell length	0.9-2.2 µm

[Ref.: #65363]	Cell width	0.6-1.2 µm

[Ref.: #65363]	Cell shape	rod-shaped
[Ref.: #65363]	Cell shape	coccus-shaped

[Ref.: #65363]	Motility	no

[Ref.: #65363]	Type of hemolysis	gamma
[Ref.: #65363]	Colony size	1-3 mm
[Ref.: #65363]	Colony color	milky-yellow
[Ref.: #65363]	Colony shape	circular
[Ref.: #65363]	Incubation period	2 days
[Ref.: #65363]	Cultivation medium used	Luria-Bertani agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65363]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #65363]

Culture medium growth

		Temperature
[Ref.: #65363]	growth	4-32 °C
[Ref.: #65363]	optimum	25-28 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #65363]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65363]			optimum	7
[Ref.: #65363]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65363]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65363]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65363]		NaCl		growth	0-2	%(w/v)
[Ref.: #65363]		NaCl		optimum	0	%(w/v)

[Ref.: #65363]

Observation

with fimbria- or pilus-like structures all over their surface

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65363]	2,3-butanediol ChEBI	-	growth
[Ref.: #65363]	4-hydroxybenzoate ChEBI	-	growth
[Ref.: #65363]	4-oxopentanoate ChEBI	-	growth
[Ref.: #65363]	acetate ChEBI	+	carbon source
[Ref.: #65363]	adipate ChEBI	-	growth
[Ref.: #65363]	azelaate ChEBI	+	carbon source
[Ref.: #65363]	benzoate ChEBI	-	growth
[Ref.: #65363]	beta-alanine ChEBI	+	carbon source
[Ref.: #65363]	casein	-	hydrolysis
[Ref.: #65363]	citraconate ChEBI	-	growth
[Ref.: #65363]	D-gluconate ChEBI	-	growth
[Ref.: #65363]	D-glucose ChEBI	-	builds acid from
[Ref.: #65363]	D-glucose ChEBI	-	growth
[Ref.: #65363]	D-malate ChEBI	+	carbon source
[Ref.: #65363]	D-ribose ChEBI	-	growth
[Ref.: #65363]	esculin ChEBI	-	hydrolysis
[Ref.: #65363]	ethanol ChEBI	-	growth
[Ref.: #65363]	gamma-aminobutyric acid ChEBI	-	growth
[Ref.: #65363]	gelatin ChEBI	-	hydrolysis
[Ref.: #65363]	gentisate ChEBI	-	growth
[Ref.: #65363]	glutaric acid ChEBI	+	carbon source
[Ref.: #65363]	histamine ChEBI	-	growth
[Ref.: #65363]	L-arabinose ChEBI	-	growth
[Ref.: #65363]	L-arginine ChEBI	+	carbon source
[Ref.: #65363]	L-aspartate ChEBI	-	growth
[Ref.: #65363]	L-histidine ChEBI	-	growth
[Ref.: #65363]	L-leucine ChEBI	-	growth
[Ref.: #65363]	L-ornithine ChEBI	-	growth
[Ref.: #65363]	L-phenylalanine ChEBI	-	growth
[Ref.: #65363]	L-tartrate ChEBI	-	growth
[Ref.: #65363]	lactate ChEBI	+	carbon source
[Ref.: #65363]	malonate ChEBI	-	growth
[Ref.: #65363]	phenylacetate ChEBI	-	growth
[Ref.: #65363]	putrescine ChEBI	-	growth
[Ref.: #65363]	sodium L-glutamate ChEBI	+	carbon source
[Ref.: #65363]	starch ChEBI	-	hydrolysis
[Ref.: #65363]	trans-aconitate ChEBI	-	growth
[Ref.: #65363]	tricarballylate ChEBI	-	growth
[Ref.: #65363]	trigonelline ChEBI	-	growth
[Ref.: #65363]	tryptamine ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65363]	acetoin ChEBI	no
[Ref.: #65363]	hydrogen sulfide ChEBI	no
[Ref.: #65363]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65363]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #65363]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65363]	catalase	+	1.11.1.6
[Ref.: #65363]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65363]	esterase Lipase (C 8)	+
[Ref.: #65363]	leucine aminopeptidase	+	3.4.1.1
[Ref.: #65363]	lipase (C 14)	+
[Ref.: #65363]	naphthol-AS-BI-phosphohydrolase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65363]	Sample type/isolated from	external lesions of diseased farmed Murray cod
[Ref.: #65363]	Sampling date	2017-04
[Ref.: #65363]	Geographic location (country and/or sea, region)	Dajing town, Yueqing City, Zhejiang province
[Ref.: #65363]	Country	China
[Ref.: #65363]	Country ISO 3 Code	CHN
[Ref.: #65363]	Continent	Asia
[Ref.: #65363]	Geographic location	28.4100°/121.1400°
[Ref.: #65363]	Enrichment culture	Columbia agar
[Ref.: #65363]	Enrichment culture composition	5% sheep blood
[Ref.: #65363]	Enrichment culture duration	3 days
[Ref.: #65363]	Enrichment culture temperature	30 ̊C
[Ref.: #65363]	Isolation/enrichment procedure	The external muscle ulcerative lesions on the fishtail of Murray cod were separated, mashed and suspended in sterilized water, then serially diluted and spread onto Columbia agar with 5 % sheep blood and aerobic incubation was performed at 30°C for 3 days

[Ref.: #67770]	Sample type/isolated from	External lesions of diseased farmed Murray cod in Dajing Town
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yueqing City, Zhejiang Province
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From external lesions of diseased farmed Murray cod(Maccullochella peelii), Intensively cultureed pond of Murray cod(E121.4
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Other	#Wound
#Infection	#Disease	-
#Host	#Fishes	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65363]	16S rRNA gene sequence	MF062566		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Acinetobacter piscicola LW15	GCA_002233755	scaffold	GenBank	2006115 tax ID
[Ref.: #66792]	Acinetobacter piscicola LW15	2791355509	draft	JGI IMG/M	2006115 tax ID	*

[Ref.: #65363]

GC-content

37.2 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65363]

Culture collection no.

MCCC 1K03337, CICC 24241, KCTC 62134, JCM 32101

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acinetobacter piscicola sp. nov., isolated from diseased farmed Murray cod (Maccullochella peelii peelii).	Liu Y, Rao Q, Tu J, Zhang J, Huang M, Hu B, Lin Q, Luo T	Int J Syst Evol Microbiol	10.1099/ijsem.0.002608	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65363

Yang Liu, Qiuhua Rao, Jiefeng Tu, Jiaonan Zhang, Minmin Huang, Bing Hu, Qiu Lin, Tuyan Luo: Acinetobacter piscicola sp. nov., isolated from diseased farmed Murray cod (Maccullochella peelii peelii). IJSEM 68: 905 - 910 2018 ( DOI 10.1099/ijsem.0.002608 , PubMed 29458492 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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