Name: Actinotalea solisilvae THG-T121
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158625

Type strain:

Species: Actinotalea solisilvae

Strain Designation: THG-T121

Culture col. no.: KACC 19191, CGMCC 4.7389, JCM 32747

Strain history: KACC 19191 <-- T.-H. Yi; Kyung Hee Univ., South Korea; THG-T121.

NCBI tax ID(s): 2072922 (species)

Section

Actinotalea solisilvae THG-T121 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from forest soil.

colony-forming
Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Cellulomonadaceae*
	Genus	*Actinotalea*
	Species	**Actinotalea solisilvae**
	Full Scientific Name (LPSN)	Actinotalea solisilvae Yan et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65350]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65350]	Cell length	1-1.2 µm

[Ref.: #65350]	Cell width	0.6-0.8 µm

[Ref.: #65350]	Cell shape	rod-shaped

[Ref.: #65350]	Motility	no

[Ref.: #65350]	Colony size	2 mm
[Ref.: #65350]	Colony color	yellow
[Ref.: #65350]	Colony shape	circular
[Ref.: #65350]	Incubation period	2 days
[Ref.: #65350]	Cultivation medium used	nutrient agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

tryptic soy agar

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

		Temperature
[Ref.: #65350]	growth	10-40 °C
[Ref.: #65350]	optimum	28-30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #65350]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65350]			optimum	7
[Ref.: #65350]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

no Confidence in %99.793

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65350]		NaCl		growth	0-4	%
[Ref.: #65350]		NaCl		optimum	1	%

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Observation

quinones: MK-10(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65350]	2-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #65350]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #65350]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65350]	4-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65350]	5-dehydro-D-gluconate ChEBI	+	assimilation
[Ref.: #65350]	adipate ChEBI	-	assimilation
[Ref.: #65350]	casein	-	hydrolysis
[Ref.: #65350]	cellulose ChEBI	+	hydrolysis
[Ref.: #65350]	chitin ChEBI	-	hydrolysis
[Ref.: #65350]	citrate ChEBI	+	assimilation
[Ref.: #65350]	D-glucose ChEBI	-	assimilation
[Ref.: #65350]	D-mannitol ChEBI	-	assimilation
[Ref.: #65350]	D-mannose ChEBI	-	assimilation
[Ref.: #65350]	D-ribose ChEBI	+	assimilation
[Ref.: #65350]	D-sorbitol ChEBI	+	assimilation
[Ref.: #65350]	decanoate ChEBI	-	assimilation
[Ref.: #65350]	dna ChEBI	-	hydrolysis
[Ref.: #65350]	esculin ChEBI	+	hydrolysis
[Ref.: #65350]	gelatin ChEBI	+	hydrolysis
[Ref.: #65350]	gluconate ChEBI	+	assimilation
[Ref.: #65350]	glucose ChEBI	+	builds acid from
[Ref.: #65350]	glycogen ChEBI	+	assimilation
[Ref.: #65350]	itaconate ChEBI	-	assimilation
[Ref.: #65350]	L-alanine ChEBI	+	assimilation
[Ref.: #65350]	L-arabinose ChEBI	+	assimilation
[Ref.: #65350]	L-fucose ChEBI	-	assimilation
[Ref.: #65350]	L-histidine ChEBI	-	assimilation
[Ref.: #65350]	L-proline ChEBI	+	assimilation
[Ref.: #65350]	L-rhamnose ChEBI	+	assimilation
[Ref.: #65350]	L-serine ChEBI	-	assimilation
[Ref.: #65350]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #65350]	lactate ChEBI	+	assimilation
[Ref.: #65350]	malate ChEBI	+	assimilation
[Ref.: #65350]	maltose ChEBI	+	assimilation
[Ref.: #65350]	melibiose ChEBI	+	assimilation
[Ref.: #65350]	myo-inositol ChEBI	+	assimilation
[Ref.: #65350]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65350]	nitrate ChEBI	-	reduction
[Ref.: #65350]	phenylacetate ChEBI	-	assimilation
[Ref.: #65350]	propionate ChEBI	+	assimilation
[Ref.: #65350]	salicin ChEBI	+	assimilation
[Ref.: #65350]	sodium acetate ChEBI	+	assimilation
[Ref.: #65350]	sodium citrate ChEBI	-	assimilation
[Ref.: #65350]	sodium malonate ChEBI	+	assimilation
[Ref.: #65350]	starch ChEBI	+	hydrolysis
[Ref.: #65350]	suberic acid ChEBI	+	assimilation
[Ref.: #65350]	sucrose ChEBI	+	assimilation
[Ref.: #65350]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65350]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65350]	valerate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65350]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65350]	acid phosphatase	+	3.1.3.2
[Ref.: #65350]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65350]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65350]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65350]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65350]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65350]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65350]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65350]	beta-galactosidase	+	3.2.1.23
[Ref.: #65350]	beta-glucosidase	+	3.2.1.21
[Ref.: #65350]	beta-glucuronidase	+	3.2.1.31
[Ref.: #65350]	catalase	+	1.11.1.6
[Ref.: #65350]	cystine arylamidase	+	3.4.11.3
[Ref.: #65350]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65350]	esterase (C 4)	+
[Ref.: #65350]	esterase Lipase (C 8)	+
[Ref.: #65350]	leucine arylamidase	+	3.4.11.1
[Ref.: #65350]	lipase (C 14)	-
[Ref.: #65350]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #65350]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65350]	trypsin	-	3.4.21.4
[Ref.: #65350]	urease	+	3.5.1.5
[Ref.: #65350]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65350]

Fatty acid	Percentage
C15:0 anteiso	34.2
C15:1 anteiso A	19.4
C16:0	15.6
C16:0 iso	8.6
C17:0 anteiso	6.9
C17:0 iso	5.6
C14:0 iso	3.5
C15:0 iso	3.1
C14:0	1.5
C18:0	1.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65350]	Sample type/isolated from	forest soil
[Ref.: #65350]	Sampling date	2016-09-21
[Ref.: #65350]	Geographic location (country and/or sea, region)	Kyung Hee University, Yongin
[Ref.: #65350]	Country	Republic of Korea
[Ref.: #65350]	Country ISO 3 Code	KOR
[Ref.: #65350]	Continent	Asia
[Ref.: #65350]	Geographic location	38.2566°/126.4360°
[Ref.: #65350]	Enrichment culture	nutrient agar
[Ref.: #65350]	Enrichment culture duration	1 week
[Ref.: #65350]	Enrichment culture temperature	28 ̊C
[Ref.: #65350]	Isolation/enrichment procedure	soil sample (1 g) was suspended in 9 ml 0.85 % (w/v) NaCl solution and mixed in a shaker for 30 min. Aliquots of the serially diluted sample were spread on nutrient agar plates and incubated at 28°C for 1 week.

[Ref.: #67770]	Sample type/isolated from	Forest soil at Kyung Hee University
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yongin
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65350]	16S rRNA gene sequence	KY287246		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinotalea solisilvae KACC 19191	GCA_016464425	contig	GenBank	2072922 tax ID	*
[Ref.: #66792]	Actinotalea solisilvae strain KACC 19191	2072922.3	wgs	PATRIC	2072922 tax ID	*

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GC-content

72.4 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinotalea solisilvae strain KACC 19191 PATRIC: 2072922.3
[Ref.: #69481]	spore-forming	no	77	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinotalea solisilvae strain KACC 19191 PATRIC: 2072922.3
[Ref.: #69480]	motile	yes	76.204	no
[Ref.: #69480]	gram-positive	yes	90.921	no
[Ref.: #69480]	anaerobic	no	98.663	yes
[Ref.: #69480]	aerobic	yes	89.188	yes
[Ref.: #69480]	halophile	no	95.601	yes
[Ref.: #69480]	spore-forming	no	88.039	no
[Ref.: #69480]	glucose-util	yes	82.393	yes
[Ref.: #69480]	flagellated	no	92.468	yes
[Ref.: #69480]	thermophile	no	95.516	yes
[Ref.: #69480]	glucose-ferment	no	70.338	no

Availability in culture collections External links

[Ref.: #65350]

Culture collection no.

KACC 19191, CGMCC 4.7389, JCM 32747

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea.	Yan ZF, Lin P, Li CT, Kook M, Yi TH	Int J Syst Evol Microbiol	10.1099/ijsem.0.002584	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65350

Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Tae-Hoo Yi: Actinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea. IJSEM 68: 788 - 794 2018 ( DOI 10.1099/ijsem.0.002584 , PubMed 29458505 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Actinotalea solisilvae strain KACC 19191

Actinotalea solisilvae strain KACC 19191

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