Strain identifier

BacDive ID: 158625

Type strain: Yes

Species: Actinotalea solisilvae

Strain Designation: THG-T121

Strain history: KACC 19191 <-- T.-H. Yi; Kyung Hee Univ., South Korea; THG-T121.

NCBI tax ID(s): 2072922 (species)

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General

@ref: 65350

BacDive-ID: 158625

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped, colony-forming

description: Actinotalea solisilvae THG-T121 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 2072922
  • Matching level: species

strain history

  • @ref: 67770
  • history: KACC 19191 <-- T.-H. Yi; Kyung Hee Univ., South Korea; THG-T121.

doi: 10.13145/bacdive158625.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Actinotalea
  • species: Actinotalea solisilvae
  • full scientific name: Actinotalea solisilvae Yan et al. 2018

@ref: 65350

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Cellulomonadaceae

genus: Actinotalea

species: Actinotalea solisilvae

strain designation: THG-T121

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
65350positive1-1.2 µm0.6-0.8 µmrod-shapedno
69480positive100

colony morphology

  • @ref: 65350
  • colony size: 2 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 2 days
  • medium used: nutrient agar

Culture and growth conditions

culture medium

@refnamegrowth
65350Nutrient agar (NA)yes
65350Reasoner's 2A agar (R2A)yes
65350Marine agar (MA)yes
65350tryptic soy agaryes
65350LB (Luria-Bertani) MEDIUMyes
65350MacConkey agarno

culture temp

@refgrowthtypetemperaturerange
65350positivegrowth10-40
65350positiveoptimum28-30mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
65350positivegrowth6-8
65350positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 65350
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.793

halophily

@refsaltgrowthtested relationconcentration
65350NaClpositivegrowth0-4 %
65350NaClpositiveoptimum1 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65350168082-dehydro-D-gluconate-assimilation
65350161933-hydroxybenzoate-assimilation
65350370543-hydroxybutyrate+assimilation
65350167244-hydroxybutyrate-assimilation
65350581435-dehydro-D-gluconate+assimilation
6535017128adipate-assimilation
6535027689decanoate-assimilation
65350casein-hydrolysis
6535062968cellulose+hydrolysis
6535017029chitin-hydrolysis
6535016947citrate+assimilation
6535017634D-glucose-assimilation
6535016899D-mannitol-assimilation
6535016024D-mannose-assimilation
6535016988D-ribose+assimilation
6535017924D-sorbitol+assimilation
6535024996lactate+assimilation
6535016991dna-hydrolysis
653504853esculin+hydrolysis
653505291gelatin+hydrolysis
6535024265gluconate+assimilation
6535017234glucose+builds acid from
6535028087glycogen+assimilation
6535017268myo-inositol+assimilation
6535017240itaconate-assimilation
6535016977L-alanine+assimilation
6535030849L-arabinose+assimilation
6535018287L-fucose-assimilation
6535015971L-histidine-assimilation
6535017203L-proline+assimilation
6535062345L-rhamnose+assimilation
6535017115L-serine-assimilation
6535017895L-tyrosine-hydrolysis
6535025115malate+assimilation
6535017306maltose+assimilation
6535028053melibiose+assimilation
65350506227N-acetylglucosamine+assimilation
6535017632nitrate-reduction
6535018401phenylacetate-assimilation
6535017272propionate+assimilation
6535017814salicin+assimilation
6535032954sodium acetate+assimilation
6535053258sodium citrate-assimilation
6535062983sodium malonate+assimilation
6535028017starch+hydrolysis
653509300suberic acid+assimilation
6535017992sucrose+assimilation
6535053424tween 20-hydrolysis
6535053426tween 80-hydrolysis
6535031011valerate+assimilation

metabolite production

  • @ref: 65350
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65350cytochrome oxidase+1.9.3.1
65350catalase+1.11.1.6
65350urease+3.5.1.5
65350alkaline phosphatase+3.1.3.1
65350esterase (C 4)+
65350esterase Lipase (C 8)+
65350leucine arylamidase+3.4.11.1
65350cystine arylamidase+3.4.11.3
65350acid phosphatase+3.1.3.2
65350naphthol-AS-BI-phosphohydrolase+
65350beta-galactosidase+3.2.1.23
65350beta-glucuronidase+3.2.1.31
65350alpha-glucosidase+3.2.1.20
65350beta-glucosidase+3.2.1.21
65350N-acetyl-beta-glucosaminidase+3.2.1.52
65350arginine dihydrolase-3.5.3.6
65350lipase (C 14)-
65350valine arylamidase-
65350trypsin-3.4.21.4
65350alpha-chymotrypsin-3.4.21.1
65350alpha-galactosidase-3.2.1.22
65350alpha-mannosidase-3.2.1.24
65350alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65350C14:01.5
    65350C16:015.6
    65350C18:01.3
    65350C14:0 iso3.5
    65350C15:0 iso3.1
    65350C16:0 iso8.6
    65350C17:0 iso5.6
    65350C15:0 anteiso34.2
    65350C15:1 anteiso A19.4
    65350C17:0 anteiso6.9
  • type of FA analysis: whole cell analysis
  • incubation medium: nutrient agar
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 4
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
65350forest soil2016-09-21Kyung Hee University, YonginRepublic of KoreaKORAsia38.2566126.436nutrient agar1 week28soil sample (1 g) was suspended in 9 ml 0.85 % (w/v) NaCl solution and mixed in a shaker for 30 min. Aliquots of the serially diluted sample were spread on nutrient agar plates and incubated at 28°C for 1 week.
67770Forest soil at Kyung Hee UniversityYonginRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Sequence information

16S sequences

  • @ref: 65350
  • description: 16S rRNA gene sequence
  • accession: KY287246
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinotalea solisilvae KACC 19191GCA_016464425contigncbi2072922
66792Actinotalea solisilvae strain KACC 191912072922.3wgspatric2072922

GC content

  • @ref: 67770
  • GC-content: 72.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno77no
motileyes76.204no
gram-positiveyes90.921no
anaerobicno98.663yes
aerobicyes89.188yes
halophileno95.601yes
spore-formingno88.039no
glucose-utilyes82.393yes
flagellatedno92.468yes
thermophileno95.516yes
glucose-fermentno70.338no

External links

@ref: 65350

culture collection no.: KACC 19191, CGMCC 4.7389, JCM 32747

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458505
  • title: Actinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea.
  • authors: Yan ZF, Lin P, Li CT, Kook M, Yi TH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002584
  • year: 2018
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Glycolipids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65350Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Tae-Hoo YiActinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea10.1099/ijsem.0.002584IJSEM 68: 788-794 201829458505
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes