Name: Sphaerisporangium rhizosphaerae NEAU-mq3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158369

Type strain:

Species: Sphaerisporangium rhizosphaerae

Strain Designation: NEAU-mq3

Culture col. no.: DSM 106198, CGMCC 4.7429, JCM 32389

Strain history: J. Zhao; Sch. of Life Sci., Northeast Agric. Univ., China; NEAU-mq3.

NCBI tax ID(s): 2269375 (species)

SI-ID 403352

DSM 106198

Special Collections

Section

Sphaerisporangium rhizosphaerae NEAU-mq3 is an aerobe, Gram-positive bacterium that was isolated from rhizosphere soil of a rubber tree.

Gram-positive
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Sphaerisporangium*
	Species	**Sphaerisporangium rhizosphaerae**
	Full Scientific Name (LPSN)	Sphaerisporangium rhizosphaerae Mu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66645]	Gram stain	positive

[Ref.: #66645]	Motility	no

[Ref.: #66645]	Multicellular complex forming ability	yes
[Ref.: #66645]	Multicellular complex color	yellowish pink
[Ref.: #66645]	Medium Name (multicellularity)	ISP3
[Ref.: #66645]	Multicellular complex further description	actinomycete which forms deep yellowish pink substrate mycelium on ISP 3 medium. Spherical sporangia are borne on aerial mycelia.

Multimedia content

[Ref.: #65045]

Caption:

Medium 65 28°C

[Ref.: #65045]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65045]

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

[Ref.: #65045]

Culture medium growth

[Ref.: #65045]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65045]

Culture medium composition

expand / minimize

[Ref.: #65045]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #65045]

Culture medium growth

[Ref.: #65045]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65045]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65045]	growth	28 °C
[Ref.: #66645]	optimum	28 °C
[Ref.: #66645]	growth	18-37 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #66645]			optimum	7
[Ref.: #66645]			growth	7-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66645]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66645]		NaCl		growth	0-1	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₂), MK-9(H₄), MK-9(H₀)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66645]	cellulose ChEBI	-	degradation
[Ref.: #66645]	creatine ChEBI	+	nitrogen source
[Ref.: #66645]	D-fructose ChEBI	-	carbon source
[Ref.: #66645]	D-galactose ChEBI	-	carbon source
[Ref.: #66645]	D-glucose ChEBI	+	carbon source
[Ref.: #66645]	D-mannitol ChEBI	-	carbon source
[Ref.: #66645]	D-mannose ChEBI	-	carbon source
[Ref.: #66645]	D-ribose ChEBI	-	carbon source
[Ref.: #66645]	D-sorbitol ChEBI	-	carbon source
[Ref.: #66645]	D-xylose ChEBI	-	carbon source
[Ref.: #66645]	gelatin ChEBI	-	hydrolysis
[Ref.: #66645]	glycine ChEBI	-	nitrogen source
[Ref.: #66645]	L-alanine ChEBI	+	nitrogen source
[Ref.: #66645]	L-arabinose ChEBI	-	carbon source
[Ref.: #66645]	L-arginine ChEBI	+	nitrogen source
[Ref.: #66645]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #66645]	L-aspartate ChEBI	-	nitrogen source
[Ref.: #66645]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #66645]	L-glutamine ChEBI	-	nitrogen source
[Ref.: #66645]	L-proline ChEBI	-	nitrogen source
[Ref.: #66645]	L-rhamnose ChEBI	+	carbon source
[Ref.: #66645]	L-serine ChEBI	+	nitrogen source
[Ref.: #66645]	L-threonine ChEBI	+	nitrogen source
[Ref.: #66645]	L-tyrosine ChEBI	+	nitrogen source
[Ref.: #66645]	lactose ChEBI	-	carbon source
[Ref.: #66645]	maltose ChEBI	+	carbon source
[Ref.: #66645]	milk	-	assimilation
[Ref.: #66645]	myo-inositol ChEBI	-	carbon source
[Ref.: #66645]	nitrate ChEBI	-	reduction
[Ref.: #66645]	raffinose ChEBI	-	carbon source
[Ref.: #66645]	starch ChEBI	+	hydrolysis
[Ref.: #66645]	sucrose ChEBI	-	carbon source
[Ref.: #66645]	tween 20 ChEBI	-	hydrolysis
[Ref.: #66645]	tween 40 ChEBI	+	hydrolysis
[Ref.: #66645]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66645]		hydrogen sulfide ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66645]		catalase	-	1.11.1.6
[Ref.: #66645]		urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65045]	Sample type/isolated from	rhizosphere soil of a rubber tree
[Ref.: #65045]	Geographic location (country and/or sea, region)	Mount Xianglu, Harbin city, Heilongjiang province (44° 4' N, 125° 42' E)
[Ref.: #65045]	Country	China
[Ref.: #65045]	Country ISO 3 Code	CHN
[Ref.: #65045]	Continent	Asia
[Ref.: #65045]	Geographic location	44.0667°/125.7000°

[Ref.: #66645]	Sample type/isolated from	rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg)
[Ref.: #66645]	Geographic location (country and/or sea, region)	Xianglu Mountain in Heilongjiang Province
[Ref.: #66645]	Country	China
[Ref.: #66645]	Country ISO 3 Code	CHN
[Ref.: #66645]	Continent	Asia
[Ref.: #66645]	Enrichment culture	humic acid-vitamin agar
[Ref.: #66645]	Enrichment culture composition	supplemented with cycloheximide (50 mg l-1) and nalidixic acid (20 mg l-1)
[Ref.: #66645]	Enrichment culture duration	21 days
[Ref.: #66645]	Enrichment culture temperature	28 ̊C
[Ref.: #66645]	Isolation/enrichment procedure	The rhizosphere soil sample was air-dried at room temperature 28°C for 14 days. After drying, 5 g of the soil sample was mixed with 45 ml distilled water and shaken on a rotary shaker at 180 r.p.m. and 28°C for 30 min. A 200 µl sample of the suspension was then spread on humic acid-vitamin agar for 21 days at 28°C.

[Ref.: #67770]	Sample type/isolated from	Rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg) from Xianglu Mountain in Heilongjiang Province
[Ref.: #67770]	Host species	Hevea brasiliensis
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MF981162 (>99% sequence identity) for Sphaerisporangium dianthi from Microbeatlas

Aquatic Samples	1
Soil Samples	10
Animal Samples
Plant Samples	12
Total Samples	23

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66645]	Sphaerisporangium rhizosphaerae strain NEAU-mq3 16S ribosomal RNA gene, partial sequence	MF981162	1511	GenBank ENA	2269375 tax ID

[Ref.: #66645]

GC-content

74.4 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65045]

Culture collection no.

DSM 106198, CGMCC 4.7429, JCM 32389

[Ref.: #111174]

SI-ID 403352

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphaerisporangium rhizosphaerae sp. nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg).	Mu S, Zhao Q, Zhao J, Cao T, Zhao X, Guo X, Li Y, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.002912	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65045

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106198

#66645

Shan Mu, Qiannan Zhao, Junwei Zhao, Tingting Cao, Xueli Zhao, Xiaowei Guo, Yuanyuan Li, Wensheng Xiang, Xiangjing Wang: Sphaerisporangium rhizosphaerae sp. nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg). IJSEM 68: 2860 - 2865 2018 ( DOI 10.1099/ijsem.0.002912 , PubMed 30010529 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#111174

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403352.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Sphaerisporangium rhizosphaerae in external resources:
SILVA
BRENDA
PANGAEA
GBIF