Name: Desulfothermobacter acidiphilus 3408-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158353

Type strain:

Species: Desulfothermobacter acidiphilus

Strain Designation: 3408-1

Culture col. no.: DSM 105356, VKM B-3183

Strain history: <- E. Frolov; Winogradsky Institute of Microbiology, Research Center of Biotechnology, RAS., Moscow, RUSSIA; 3408-1 <- E. N. Frolov {2016}

NCBI tax ID(s): 1938353 (species)

SI-ID 406469

DSM 105356

Special Collections

Section

Desulfothermobacter acidiphilus 3408-1 is an anaerobe, spore-forming, Gram-positive bacterium that was isolated from terrestrial hot spring.

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	*Thermoanaerobacteraceae*
	Genus	*Desulfothermobacter*
	Species	**Desulfothermobacter acidiphilus**
	Full Scientific Name (LPSN)	Desulfothermobacter acidiphilus Frolov et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65358]	Gram stain	positive

[Ref.: #65358]	Cell length	2-5 µm

[Ref.: #65358]	Cell width	0.6-0.7 µm

[Ref.: #65358]	Cell shape	rod-shaped

[Ref.: #65358]	Motility	yes

[Ref.: #65358]	Flagellum arrangement	peritrichous

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65029]

Culture medium

DESULFOTHERMOBACTER MEDIUM (DSMZ Medium 1675)

[Ref.: #65029]

Culture medium growth

[Ref.: #65029]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65029]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65029]	growth	55 °C
[Ref.: #65358]	growth	42-70 °C
[Ref.: #65358]	optimum	55 °C

	pH	Kind of pH		pH
[Ref.: #65358]			optimum	4.5
[Ref.: #65358]			growth	2.9-6.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65358]	Oxygen tolerance	anaerobe
[Ref.: #65358]	Oxygen tolerance	microaerophile

[Ref.: #65358]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65358]		NaCl		growth	0-2	%(w/v)
[Ref.: #65358]		NaCl	no	growth	>2	%(w/v)

[Ref.: #65358]	Observation	Moderately thermoacidophilic
[Ref.: #65358]	Observation	The doubling time is 14 h under optimum growth conditions.
[Ref.: #65358]	Observation	Facultatively chemolithoautotrophic

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65358]	acetate ChEBI	-	growth
[Ref.: #65358]	butyrate ChEBI	-	growth
[Ref.: #65358]	citrate ChEBI	-	growth
[Ref.: #65358]	dioxygen ChEBI	-	electron acceptor
[Ref.: #65358]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #65358]	ethanol ChEBI	-	growth
[Ref.: #65358]	ferric iron ChEBI	-	electron acceptor
[Ref.: #65358]	fumarate ChEBI	-	electron acceptor
[Ref.: #65358]	fumarate ChEBI	-	growth
[Ref.: #65358]	glucose ChEBI	+	fermentation
[Ref.: #65358]	glycerol ChEBI	-	growth
[Ref.: #65358]	lactate ChEBI	-	growth
[Ref.: #65358]	lactose ChEBI	-	growth
[Ref.: #65358]	malate ChEBI	-	growth
[Ref.: #65358]	maltose ChEBI	+	fermentation
[Ref.: #65358]	methanol ChEBI	-	growth
[Ref.: #65358]	nitrate ChEBI	-	electron acceptor
[Ref.: #65358]	nitrite ChEBI	-	electron acceptor
[Ref.: #65358]	peptone	-	growth
[Ref.: #65358]	propanol ChEBI	-	growth
[Ref.: #65358]	propionate ChEBI	-	growth
[Ref.: #65358]	pyruvate ChEBI	+	fermentation
[Ref.: #65358]	succinate ChEBI	-	growth
[Ref.: #65358]	sucrose ChEBI	+	fermentation
[Ref.: #65358]	sulfate ChEBI	+	electron acceptor
[Ref.: #65358]	sulfite ChEBI	-	electron acceptor
[Ref.: #65358]	thiosulfate ChEBI	+	electron acceptor
[Ref.: #65358]	yeast extract	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65029]	Sample type/isolated from	terrestrial hot spring
[Ref.: #65029]	Geographic location (country and/or sea, region)	Kamchatka, Uzon Caldera, Kronotsky Nature Reserve
[Ref.: #65029]	Country	Russia
[Ref.: #65029]	Country ISO 3 Code	RUS
[Ref.: #65029]	Continent	Europe
[Ref.: #65029]	Geographic location	52.5348°/158.1980°

[Ref.: #65358]	Sample type/isolated from	terrestrial hot spring, elevation 794 m
[Ref.: #65358]	Sampling date	2014-08
[Ref.: #65358]	Geographic location (country and/or sea, region)	foot of Mutnovskii volcano, Kamchatka
[Ref.: #65358]	Country	Russia
[Ref.: #65358]	Country ISO 3 Code	RUS
[Ref.: #65358]	Continent	Asia
[Ref.: #65358]	Geographic location	52.5347°/158.1970°
[Ref.: #65358]	Enrichment culture	liquid medium
[Ref.: #65358]	Enrichment culture composition	(g l-1): NH4Cl, 0.33; KCl, 0.33; MgCl2 x 2H2O, 0.33; CaCl2 x 6H2O, 0.33; KH2PO4, 0.33; Na2SO4, 2.8; Na2S9H2O, 0.5; 1 ml trace element solution [8]; vitamin solution [9]; 1.0 mg l-1 resazurin. Sodium sulfide was used as a reducing agent. Resazurin was added as a redox indicator. To adjust the pH of the medium to 4.5, 3 N HCl was used.
[Ref.: #65358]	Enrichment culture duration	4 days
[Ref.: #65358]	Enrichment culture temperature	55 ̊C
[Ref.: #65358]	Isolation/enrichment procedure	An enrichment culture was initiated by inoculating the sample (10 % w/v) into anaerobically prepared sterilized (by autoclaving at 121°C for 1 h) liquid medium. The medium was dispensed in 5 ml aliquots into 17 ml Hungate tubes; the head space was filled with H 2 /CO 2 (4 : 1, 151.9 kPa).
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Thermal spring

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65029]	Desulfothermobacter acidiphilus strain 3408-1 16S ribosomal RNA gene, partial sequence	KY465722	1537	GenBank ENA	1938353 tax ID

[Ref.: #65029]	GC-content	58 mol%
[Ref.: #65358]	GC-content	58 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65029]

Culture collection no.

DSM 105356, VKM B-3183

[Ref.: #111158]

SI-ID 406469

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfothermobacter acidiphilus gen. nov., sp. nov., a thermoacidophilic sulfate-reducing bacterium isolated from a terrestrial hot spring.	Frolov EN, Zayulina KS, Kopitsyn DS, Kublanov IV, Bonch-Osmolovskaya EA, Chernyh NA	Int J Syst Evol Microbiol	10.1099/ijsem.0.002599	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65029

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105356

#65358

E. N. Frolov, K. S. Zayulina, D. S. Kopitsyn, I. V. Kublanov, E. A. Bonch-Osmolovskaya, N. A. Chernyh: Desulfothermobacter acidiphilus gen. nov., sp. nov., a thermoacidophilic sulfate-reducing bacterium isolated from a terrestrial hot spring. IJSEM 68: 871 - 875 2018 ( DOI 10.1099/ijsem.0.002599 , PubMed 29458537 )

#111158

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406469.1 )

* These data were automatically processed and therefore are not curated

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