Methanolobus psychrotolerans (DSM 104044, BCRC AR10049, NBRC 112514)

Name: Methanolobus psychrotolerans (DSM 104044, BCRC AR10049, NBRC 112514)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158352

Type strain

Strain Designation YSF-03

Culture col. no. DSM 104044 BCRC AR10049 NBRC 112514 YSF-03

NCBI tax ID(s) 1874706

Special Collections DSMZ Literature strains

History <- M.-C. Lai, National Chung Hsing Univ., Dept. of Life Scs., Taiwan; YSF-03 <- S.-L. Tang and A. G. Degermendzhy, Siberian Federal University, Krasnoyarsk, Russia {2014}

Links

Methanolobus psychrotolerans YSF-03 is an obligate anaerobe, mesophilic, Gram-negative prokaryote that was isolated from sediment of saline lake.

Gram-negative coccus-shaped obligate anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 400903

NBRC 112514,DSM 104044

@ref 20215

Last LPSN update 2026-02-09 11:38:18

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanolobus

Species Methanolobus psychrotolerans

Full scientific name Methanolobus psychrotolerans Chen et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Motility
65460	negative	0.8-1.2 µm	coccus-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65028	METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)		Medium recipe at MediaDive	Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
65028	positive	growth	35	mesophilic
65460	positive	growth	0-37
65460	positive	optimum	30-37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
65460	positive	optimum	7-7.4
65460	positive	growth	6-8.9	alkaliphile

Physiology and metabolism

Oxygen tolerance

65460

Oxygen toleranceobligate anaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65460	NaCl	positive	growth	0-0.85 M
65460	NaCl	positive	optimum	0.17 M

Observation

@ref	Observation
65460	occur singly, in pairs, in tetra-cocci and in aggregates
65460	cell growth is completely inhibited by chloramphenicol and tetracycline, but not by ampicillin, kanamycin, penicillin, spectinomycin or vancomycin

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65460	16526 ChEBI	carbon dioxide	-	assimilation
65460	18276 ChEBI	dihydrogen	-	assimilation
65460	17437 ChEBI	dimethyl sulfide	-	assimilation
65460	17170 ChEBI	dimethylamine	-	assimilation
65460	17790 ChEBI	methanol	+	assimilation
65460	16830 ChEBI	methylamine	-	assimilation
65460	32954 ChEBI	sodium acetate	-	assimilation
65460	62965 ChEBI	sodium formate	-	assimilation
65460	18139 ChEBI	trimethylamine	+	assimilation

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Isolation procedure
65028	sediment of saline lake		East Siberia, Republic of Khakassia, Lake Shira	Russia	RUS	Europe	54.5167	90.2 54.5167/90.2
65460	sediment samples of the saline meromictic Lake Shira	July 2010	Republic of Khakassia, Siberia	Russia	RUS	Asia	54.5097	90.1922 54.5097/90.1922	one to two ml of the sediment samples were immediately inoculated into 20 ml MB/W enrichment medium with acetate (50 mM), formate (100 mM) or methanol (50 mM) as catabolic substrates in 60 ml serum bottles sealed with butyl rubber stoppers and aluminium caps. These samples were incubated at room temperature in the laboratory

Taxonmaps

69479

Global distribution of 16S sequence KX573102 (>99% sequence identity) for Methanolobus psychrotolerans subclade from Microbeatlas

Aquatic Samples	8
Soil Samples	1
Animal Samples
Plant Samples	4
Total Samples	13

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM224304v1 assembly for Methanolobus psychrotolerans YSF-03	contig	GCA_002243045	1874706.3	8058276500	1874706	52.3

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
65028	Methanolobus psychrotolerans strain YSF-03 16S ribosomal RNA gene, partial sequence	KX573102	1404		1874706

GC content

@ref	GC-content (mol%)	Method
65028	41.3	sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanolobus psychrotolerans YSF-03
NCBI: GCA_002243045.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	68.31	no
125438	anaerobic	anaerobicⓘ	yes	89.25	yes
125438	aerobic	aerobicⓘ	no	93.18	yes
125438	spore-forming	spore-formingⓘ	no	83.69	no
125438	thermophilic	thermophileⓘ	no	82.65	no
125438	flagellated	motile2+ⓘ	no	81.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanolobus psychrotolerans sp. nov., a psychrotolerant methanoarchaeon isolated from a saline meromictic lake in Siberia.	Chen SC, Huang HH, Lai MC, Weng CY, Chiu HH, Tang SL, Rogozin DY, Degermendzhy AG	Int J Syst Evol Microbiol	10.1099/ijsem.0.002685	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65028	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104044
#65460	Sheng-Chung Chen, Hsing-Hua Huang, Mei-Chin Lai, Chieh-Yin Weng, Hsiu-Hui Chiu, Sen-Lin Tang, Denis Yu Rogozin, Andrey G. Degermendzhy: Methanolobus psychrotolerans sp. nov., a psychrotolerant methanoarchaeon isolated from a saline meromictic lake in Siberia. IJSEM 68: 1378 - 1383 2018 ( DOI 10.1099/ijsem.0.002685 , PubMed 29509131 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanolobus psychrotolerans (DSM 104044, BCRC AR10049, NBRC 112514)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_002243045

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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