Name: Proteiniborus indolifex BA2-13
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158351

Type strain:

Species: Proteiniborus indolifex

Strain Designation: BA2-13

Culture col. no.: DSM 103060, LMG 29818

Strain history: <- S. Hahnke, Institut für Agrartechnik Bornim e.V.; BA2-13

NCBI tax ID(s): 1776760 (species)

SI-ID 407087

DSM 103060

Section

Proteiniborus indolifex BA2-13 is an obligate anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from thermophilic biogas plant.

colony-forming
Gram-positive
rod-shaped
mesophilic
obligate anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Proteiniborus*
	Species	**Proteiniborus indolifex**
	Full Scientific Name (LPSN)	Proteiniborus indolifex Hahnke et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65484]	Gram stain	positive

[Ref.: #65484]	Cell length	1.8-8.5 µm

[Ref.: #65484]	Cell width	0.6-0.8 µm

[Ref.: #65484]	Cell shape	rod-shaped

[Ref.: #65484]	Colony size	1.2 mm
[Ref.: #65484]	Colony color	light beige
[Ref.: #65484]	Colony shape	circular
[Ref.: #65484]	Cultivation medium used	Columbia blood agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Columbia blood agar

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Culture medium growth

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Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #65027]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65027]	growth	37 °C
[Ref.: #65484]	growth	25-50 °C
[Ref.: #65484]	optimum	35-50 °C

[Ref.: #65027]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #65484]			optimum	7.6
[Ref.: #65484]			growth	6.3-9.5

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

obligate anaerobe

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Observation

major polar lipids are diphosphatidylglycerol, glycolipids, phospholipids and phosphatidylgycerol

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65484]	arabinose ChEBI	-	growth
[Ref.: #65484]	cellobiose ChEBI	-	assimilation
[Ref.: #65484]	cellobiose ChEBI	-	growth
[Ref.: #65484]	D-glucose ChEBI	-	assimilation
[Ref.: #65484]	D-mannitol ChEBI	-	assimilation
[Ref.: #65484]	D-mannose ChEBI	-	assimilation
[Ref.: #65484]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65484]	D-xylose ChEBI	-	assimilation
[Ref.: #65484]	esculin ChEBI	-	hydrolysis
[Ref.: #65484]	ethanol ChEBI	-	growth
[Ref.: #65484]	fructose ChEBI	-	growth
[Ref.: #65484]	fucose ChEBI	-	growth
[Ref.: #65484]	gelatin ChEBI	+	hydrolysis
[Ref.: #65484]	glycerol ChEBI	-	assimilation
[Ref.: #65484]	L-arabinose ChEBI	-	assimilation
[Ref.: #65484]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65484]	lactose ChEBI	-	assimilation
[Ref.: #65484]	lactose ChEBI	-	growth
[Ref.: #65484]	maltose ChEBI	-	assimilation
[Ref.: #65484]	maltose ChEBI	-	growth
[Ref.: #65484]	melezitose ChEBI	-	assimilation
[Ref.: #65484]	raffinose ChEBI	-	assimilation
[Ref.: #65484]	salicin ChEBI	-	assimilation
[Ref.: #65484]	starch ChEBI	-	growth
[Ref.: #65484]	succinamate ChEBI	-	growth
[Ref.: #65484]	sucrose ChEBI	-	assimilation
[Ref.: #65484]	trehalose ChEBI	-	assimilation
[Ref.: #65484]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65484]	acetate ChEBI	yes
[Ref.: #65484]	carbon dioxide ChEBI	yes
[Ref.: #65484]	dihydrogen ChEBI	yes
[Ref.: #65484]	indole ChEBI	yes
[Ref.: #65484]	isobutyrate ChEBI	yes
[Ref.: #65484]	isovalerate ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #65484]	acid phosphatase	+	3.1.3.2
[Ref.: #65484]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65484]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #65484]	alpha-fucosidase	+	3.2.1.51
[Ref.: #65484]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65484]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65484]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65484]	beta-galactosidase	-	3.2.1.23
[Ref.: #65484]	beta-glucosidase	-	3.2.1.21
[Ref.: #65484]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65484]	catalase	-	1.11.1.6
[Ref.: #65484]	cystine arylamidase	+	3.4.11.3
[Ref.: #65484]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65484]	esterase (C 4)	+
[Ref.: #65484]	esterase Lipase (C 8)	+
[Ref.: #65484]	leucine arylamidase	+	3.4.11.1
[Ref.: #65484]	lipase (C 14)	+
[Ref.: #65484]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65484]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65484]	trypsin	-	3.4.21.4
[Ref.: #65484]	urease	-	3.5.1.5
[Ref.: #65484]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65484]

Fatty acid	Percentage
C15:0 iso	34.7
C14:0 iso 3OH	14.7
C14:0	9.7
iso-C15:1 H and/or C13:0 3-OH	9.6
C17:1 iso I and/or C17:1 anteiso B	9.1
C15:0 anteiso	4.3
C16:0	2.7
Unknown 13.565	2.6
C13:1ω1c	1.9
C13:0 iso	1.4
C14:0 2OH	1.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65027]	Sample type/isolated from	thermophilic biogas plant
[Ref.: #65027]	Geographic location (country and/or sea, region)	Viersen
[Ref.: #65027]	Country	Germany
[Ref.: #65027]	Country ISO 3 Code	DEU
[Ref.: #65027]	Continent	Europe

[Ref.: #65484]	Sample type/isolated from	reactor material of a thermophilic industrial-scale biogas plant, the fermenter was operated at 54°C and was fed with maize silage, pig and cow manure, and barley. A detailed description of this biogas plant as well as a polyphasic analysis of its microbial community was recently published by Maus et al.
[Ref.: #65484]	Enrichment culture duration	6 weeks
[Ref.: #65484]	Enrichment culture temperature	50 ̊C
[Ref.: #65484]	Isolation/enrichment procedure	The bacterial strain was isolated from an enrichment culture which was set up in order to cultivate methanogenic archaea. Therefore, MZ medium 287 was modified according to Stantscheff et al. and supplemented with 1 mg l^-1 Na2WO4xH2O, sodium acetate and formate (10mM each), methanol, and reactor sludge (5 % (v/v) each). Sludge was prepared as described for DSMZ medium 119. The medium was prepared under anoxic conditions in an anaerobic chamber, transferred to serum bottles and autoclaved under
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Bioreactor	-

What are isolation sources categories?

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Global distribution of 16S sequence KT351641 (>99% sequence identity) for Proteiniborus from Microbeatlas

Aquatic Samples	529
Soil Samples	922
Animal Samples	16115
Plant Samples	391
Total Samples	17957

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65027]	Proteiniborus indolifex strain BA2-13 16S ribosomal RNA gene, partial sequence	KT351641	1359	ENA	1776760 tax ID

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GC-content

33.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65027]

Culture collection no.

DSM 103060, LMG 29818

[Ref.: #111156]

SI-ID 407087

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proteiniborus indolifex sp. nov., isolated from a thermophilic industrial-scale biogas plant.	Hahnke S, Langer T, Klocke M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002591	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65027

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103060

#65484

Sarah Hahnke, Thomas Langer, Michael Klocke: Proteiniborus indolifex sp. nov., isolated from a thermophilic industrial-scale biogas plant. IJSEM 68: 824 - 828 2018 ( DOI 10.1099/ijsem.0.002591 , PubMed 29458500 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#111156

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407087.1 )

* These data were automatically processed and therefore are not curated

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