Name: Thermostaphylospora grisealba 3-12X
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158325

Type strain:

Species: Thermostaphylospora grisealba

Strain Designation: 3-12X

Culture col. no.: DSM 46781, CGMCC 4.7160

Strain history: <- H. Wu, Guangxi University,China; 3-12X

NCBI tax ID(s): 1490296 (species)

SI-ID 407274

DSM 46781

Section

Thermostaphylospora grisealba 3-12X is an aerobe, thermophilic, Gram-positive bacterium that has a brown soluble pigmentation and was isolated from mushroom compost.

Gram-positive
pigmented
aerobe
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Thermostaphylospora*
	Species	**Thermostaphylospora grisealba**
	Full Scientific Name (LPSN)	Thermostaphylospora grisealba Wu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65333]	Gram stain	positive

[Ref.: #65333]	Motility	no

[Ref.: #65333]	Pigment production	yes
[Ref.: #65333]	Pigmentation color	brown soluble

[Ref.: #65333]	Multicellular complex forming ability	yes
[Ref.: #65333]	Multicellular complex color	whitish to yellow / white to greyish white
[Ref.: #65333]	Medium Name (multicellularity)	Humic acid-vitamin medium, Czapek's medium, ISP4 and ISP7 media
[Ref.: #65333]	Multicellular complex further description	Vegetative mycelium and aerial mycelium are well developed and branched without fragmentation. Vegetative mycelium is whitish to yellow. Aerial mycelium is white to greyish white.

Multimedia content

[Ref.: #65001]

Caption:

Medium 554 45°C

[Ref.: #65001]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65001]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #65001]

Culture medium growth

[Ref.: #65001]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #65001]

Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

[Ref.: #65001]

Culture medium growth

[Ref.: #65001]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65001]

Culture medium composition

expand / minimize

[Ref.: #65333]

Culture medium

Humic acid-vitamin medium

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

Bennett's agar

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

Czapek's medium

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

ISP 3

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

ISP 7

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

Gause's asparagine agar

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

water agar

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

ISP 4

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

Sauton's agar

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

potato dextrose agar

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

ISP 2

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

ISP 6

[Ref.: #65333]

Culture medium growth

[Ref.: #65333]

Culture medium

ISP 5

[Ref.: #65333]

Culture medium growth

		Temperature
[Ref.: #65001]	growth	45 °C
[Ref.: #65333]	growth	40-60 °C
[Ref.: #65333]	optimum	45-55 °C

[Ref.: #65001]	Temperature range	thermophilic
[Ref.: #65333]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #65333]			optimum	7-10
[Ref.: #65333]			growth	6-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65333]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65333]		NaCl		growth	0-2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65333]	adenine ChEBI	-	hydrolysis
[Ref.: #65333]	allantoin ChEBI	-	hydrolysis
[Ref.: #65333]	casein	-	hydrolysis
[Ref.: #65333]	cellobiose ChEBI	-	carbon source
[Ref.: #65333]	D-fructose ChEBI	-	carbon source
[Ref.: #65333]	D-galactose ChEBI	-	carbon source
[Ref.: #65333]	D-mannose ChEBI	-	carbon source
[Ref.: #65333]	D-sorbitol ChEBI	+	carbon source
[Ref.: #65333]	D-xylose ChEBI	-	carbon source
[Ref.: #65333]	dextrin ChEBI	-	carbon source
[Ref.: #65333]	esculin ChEBI	-	hydrolysis
[Ref.: #65333]	gelatin ChEBI	-	hydrolysis
[Ref.: #65333]	glucose ChEBI	+	carbon source
[Ref.: #65333]	glycine ChEBI	-	nitrogen source
[Ref.: #65333]	guanine ChEBI	-	hydrolysis
[Ref.: #65333]	hydroxyproline ChEBI	-	nitrogen source
[Ref.: #65333]	hypoxanthine ChEBI	+	hydrolysis
[Ref.: #65333]	inulin ChEBI	-	carbon source
[Ref.: #65333]	isoleucine ChEBI	-	nitrogen source
[Ref.: #65333]	L-alanine ChEBI	-	nitrogen source
[Ref.: #65333]	L-arabinose ChEBI	+	carbon source
[Ref.: #65333]	L-arginine ChEBI	+	nitrogen source
[Ref.: #65333]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #65333]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #65333]	L-histidine ChEBI	-	nitrogen source
[Ref.: #65333]	L-lysine ChEBI	+	nitrogen source
[Ref.: #65333]	L-methionine ChEBI	+	nitrogen source
[Ref.: #65333]	L-phenylalanine ChEBI	-	nitrogen source
[Ref.: #65333]	L-proline ChEBI	+	nitrogen source
[Ref.: #65333]	L-rhamnose ChEBI	-	carbon source
[Ref.: #65333]	L-serine ChEBI	-	nitrogen source
[Ref.: #65333]	L-threonine ChEBI	-	nitrogen source
[Ref.: #65333]	L-tyrosine ChEBI	+	hydrolysis
[Ref.: #65333]	L-tyrosine ChEBI	+	nitrogen source
[Ref.: #65333]	L-valine ChEBI	-	nitrogen source
[Ref.: #65333]	lactose ChEBI	-	carbon source
[Ref.: #65333]	maltose ChEBI	-	carbon source
[Ref.: #65333]	mannitol ChEBI	-	carbon source
[Ref.: #65333]	milk	-	assimilation
[Ref.: #65333]	myo-inositol ChEBI	-	carbon source
[Ref.: #65333]	nitrate ChEBI	-	reduction
[Ref.: #65333]	raffinose ChEBI	+	carbon source
[Ref.: #65333]	ribose ChEBI	-	carbon source
[Ref.: #65333]	salicin ChEBI	+	carbon source
[Ref.: #65333]	sodium acetate ChEBI	-	carbon source
[Ref.: #65333]	sodium citrate ChEBI	+	carbon source
[Ref.: #65333]	sodium propionate ChEBI	-	carbon source
[Ref.: #65333]	starch ChEBI	-	hydrolysis
[Ref.: #65333]	sucrose ChEBI	+	carbon source
[Ref.: #65333]	trehalose ChEBI	-	carbon source
[Ref.: #65333]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65333]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65333]	tween 60 ChEBI	+	hydrolysis
[Ref.: #65333]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65333]	urea ChEBI	-	hydrolysis
[Ref.: #65333]	xanthine ChEBI	-	hydrolysis
[Ref.: #65333]	xylan ChEBI	-	hydrolysis
[Ref.: #65333]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65333]		hydrogen sulfide ChEBI	no
[Ref.: #65333]		melanin ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65333]		catalase	-	1.11.1.6
[Ref.: #65333]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65001]	Sample type/isolated from	mushroom compost
[Ref.: #65001]	Geographic location (country and/or sea, region)	Nanning, Guangxi University (22° 51' N 108° 17' E)
[Ref.: #65001]	Country	China
[Ref.: #65001]	Country ISO 3 Code	CHN
[Ref.: #65001]	Continent	Asia
[Ref.: #65001]	Geographic location	22.8500°/108.2830°

[Ref.: #65333]	Sample type/isolated from	mushroom compost
[Ref.: #65333]	Geographic location (country and/or sea, region)	Guangxi University, Nanning, Guangxi Zhuang Autonomous Region
[Ref.: #65333]	Country	China
[Ref.: #65333]	Country ISO 3 Code	CHN
[Ref.: #65333]	Continent	Asia
[Ref.: #65333]	Geographic location	22.8500°/108.2830°
[Ref.: #65333]	Enrichment culture	Humic acid-vitamin medium
[Ref.: #65333]	Enrichment culture duration	3 days
[Ref.: #65333]	Isolation/enrichment procedure	dilution plate technique, incubated in the dark and under aerobic conditions
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Host	#Fungi	#Mushroom
#Engineered	#Agriculture	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ027598 (>99% sequence identity) for Thermostaphylospora grisealba from Microbeatlas

Aquatic Samples	142
Soil Samples	1951
Animal Samples	215
Plant Samples	704
Total Samples	3012

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65001]	Thermostaphylospora grisealba strain 3-12x 16S ribosomal RNA gene, partial sequence	KJ027598	1457	ENA	1490296 tax ID

[Ref.: #65333]

GC-content

74.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65001]

Culture collection no.

DSM 46781, CGMCC 4.7160

[Ref.: #111135]

SI-ID 407274

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermostaphylospora grisealba gen. nov., sp. nov., isolated from mushroom compost and transfer of Thermomonospora chromogena Zhang et al. 1998 to Thermostaphylospora chromogena comb. nov.	Wu H, Liu B, Shao Y, Ou X, Huang F	Int J Syst Evol Microbiol	10.1099/ijsem.0.002551	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65001

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46781

#65333

Hao Wu, Bin Liu, Yuanyuan Shao, Xiaoyun Ou, Fuchang Huang: Thermostaphylospora grisealba gen. nov., sp. nov., isolated from mushroom compost and transfer of Thermomonospora chromogena Zhang et al. 1998 to Thermostaphylospora chromogena comb. nov.. IJSEM 68: 602 - 608 2018 ( DOI 10.1099/ijsem.0.002551 , PubMed 29388545 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#111135

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407274.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Thermostaphylospora grisealba in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF