Name: Cytobacillus solani 5L6
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158260

Type strain:

Species: Cytobacillus solani

Strain Designation: 5L6

Culture col. no.: DSM 104513, CICC 23855, KCTC 33663

Strain history: <- Y. Liu, China Natl. Res. Inst. of Food and Fermentation Ind., Beijing, China; 5L6 <- Y. Cao

NCBI tax ID(s): 1637975 (species)

SI-ID 403524

DSM 104513

Other strains from Cytobacillus solani

C. solani FJAT-18043, DSM 29501, CCTCC AB ... (type strain)

Section

Cytobacillus solani 5L6 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from maize seeds.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Cytobacillus*
	Species	**Cytobacillus solani**
	Full Scientific Name (LPSN)	Cytobacillus solani (Liu et al. 2015) Patel and Gupta 2020

	Synonym	Cytobacillus ciccensis
	Synonym	Bacillus ciccensis
	Synonym	Bacillus solani

Information on morphological and physiological properties Morphology

[Ref.: #65267]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65267]	Cell length	3-4 µm

[Ref.: #65267]	Cell width	0.6-0.8 µm

[Ref.: #65267]	Cell shape	rod-shaped

[Ref.: #65267]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.865

[Ref.: #65267]	Flagellum arrangement	peritrichous

[Ref.: #65267]	Colony color	creamy
[Ref.: #65267]	Colony shape	circular
[Ref.: #65267]	Cultivation medium used	tryptic soy agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64919]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #64919]

Culture medium growth

[Ref.: #64919]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64919]

Culture medium composition

expand / minimize

[Ref.: #65267]

Culture medium

tryptic soy agar

[Ref.: #65267]

Culture medium growth

		Temperature
[Ref.: #64919]	growth	30 °C
[Ref.: #65267]	growth	4-45 °C
[Ref.: #65267]	optimum	30 °C

[Ref.: #64919]	Temperature range	mesophilic
[Ref.: #65267]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65267]			optimum	7
[Ref.: #65267]			growth	6-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65267]

Oxygen tolerance

aerobe

[Ref.: #65267]	Type of spore	endospore
[Ref.: #65267]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65267]		NaCl		growth	0-9	%(w/v)
[Ref.: #65267]		NaCl		optimum	0	%(w/v)

[Ref.: #65267]

Observation

The cell-wall peptidoglycan contained ornithine, serine, aspartic acid, glutamic acid and alanine.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65267]	4-nitrophenyl beta-D-galactopyranoside ChEBI	-	hydrolysis
[Ref.: #65267]	amygdalin ChEBI	-	builds acid from
[Ref.: #65267]	arabinose ChEBI	-	assimilation
[Ref.: #65267]	arbutin ChEBI	-	builds acid from
[Ref.: #65267]	casein	-	hydrolysis
[Ref.: #65267]	cellobiose ChEBI	+	builds acid from
[Ref.: #65267]	cellobiose ChEBI	+	carbon source
[Ref.: #65267]	citrate ChEBI	-	assimilation
[Ref.: #65267]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65267]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65267]	D-fructose ChEBI	-	builds acid from
[Ref.: #65267]	D-fucose ChEBI	-	builds acid from
[Ref.: #65267]	D-galactose ChEBI	-	builds acid from
[Ref.: #65267]	D-glucose ChEBI	+	builds acid from
[Ref.: #65267]	D-glucose ChEBI	+	carbon source
[Ref.: #65267]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65267]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65267]	D-mannose ChEBI	-	builds acid from
[Ref.: #65267]	D-ribose ChEBI	+	builds acid from
[Ref.: #65267]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #65267]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65267]	D-xylose ChEBI	-	builds acid from
[Ref.: #65267]	decanoate ChEBI	-	assimilation
[Ref.: #65267]	erythritol ChEBI	-	builds acid from
[Ref.: #65267]	esculin ChEBI	+	builds acid from
[Ref.: #65267]	esculin ChEBI	+	carbon source
[Ref.: #65267]	esculin ChEBI	+	hydrolysis
[Ref.: #65267]	galactitol ChEBI	-	builds acid from
[Ref.: #65267]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65267]	glucose ChEBI	-	builds acid from
[Ref.: #65267]	glycerol ChEBI	+	builds acid from
[Ref.: #65267]	glycerol ChEBI	+	carbon source
[Ref.: #65267]	glycogen ChEBI	-	builds acid from
[Ref.: #65267]	inulin ChEBI	-	builds acid from
[Ref.: #65267]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65267]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65267]	L-fucose ChEBI	-	builds acid from
[Ref.: #65267]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65267]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65267]	L-xylose ChEBI	-	builds acid from
[Ref.: #65267]	lactose ChEBI	-	builds acid from
[Ref.: #65267]	malate ChEBI	-	assimilation
[Ref.: #65267]	maltose ChEBI	-	assimilation
[Ref.: #65267]	maltose ChEBI	+	builds acid from
[Ref.: #65267]	maltose ChEBI	+	carbon source
[Ref.: #65267]	mannitol ChEBI	-	assimilation
[Ref.: #65267]	mannitol ChEBI	+	builds acid from
[Ref.: #65267]	mannose ChEBI	-	assimilation
[Ref.: #65267]	melezitose ChEBI	-	builds acid from
[Ref.: #65267]	melibiose ChEBI	-	builds acid from
[Ref.: #65267]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65267]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65267]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65267]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65267]	myo-inositol ChEBI	+	builds acid from
[Ref.: #65267]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65267]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #65267]	nitrate ChEBI	+	reduction
[Ref.: #65267]	phenylacetate ChEBI	-	assimilation
[Ref.: #65267]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65267]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65267]	raffinose ChEBI	-	builds acid from
[Ref.: #65267]	ribitol ChEBI	-	builds acid from
[Ref.: #65267]	salicin ChEBI	+	builds acid from
[Ref.: #65267]	salicin ChEBI	+	carbon source
[Ref.: #65267]	starch ChEBI	-	builds acid from
[Ref.: #65267]	sucrose ChEBI	-	builds acid from
[Ref.: #65267]	sucrose ChEBI	+	builds acid from
[Ref.: #65267]	sucrose ChEBI	+	carbon source
[Ref.: #65267]	trehalose ChEBI	-	builds acid from
[Ref.: #65267]	turanose ChEBI	-	builds acid from
[Ref.: #65267]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65267]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65267]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65267]	acetoin ChEBI	no
[Ref.: #65267]	hydrogen sulfide ChEBI	no
[Ref.: #65267]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65267]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #65267]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65267]	catalase	+	1.11.1.6
[Ref.: #65267]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65267]	gelatinase	+
[Ref.: #65267]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65267]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65267]	tryptophan deaminase	-	4.1.99.1
[Ref.: #65267]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64919]	Sample type/isolated from	maize seeds
[Ref.: #64919]	Geographic location (country and/or sea, region)	Gansu Province (39° 04' 57.94'' N, 100° 12' 49.40'' E), Zhangye City, scientific research base of the Beijing Academy of Agriculture and Forestry Sciences (BAAFS)
[Ref.: #64919]	Country	China
[Ref.: #64919]	Country ISO 3 Code	CHN
[Ref.: #64919]	Continent	Asia
[Ref.: #64919]	Geographic location	39.0828°/100.2140°

[Ref.: #65267]	Sample type/isolated from	isolated from seeds of hybrid maize (Zea mays L., Jingke 968)
[Ref.: #65267]	Sampling date	2014-09
[Ref.: #65267]	Geographic location (country and/or sea, region)	scientific research base of the Beijing Academy of Agriculture and Forestry Sciences (BAAFS) in Gansu Province
[Ref.: #65267]	Country	China
[Ref.: #65267]	Country ISO 3 Code	CHN
[Ref.: #65267]	Continent	Asia
[Ref.: #65267]	Geographic location	39.0827°/100.2140°
[Ref.: #65267]	Enrichment culture	LB agar
[Ref.: #65267]	Enrichment culture duration	2 days
[Ref.: #65267]	Enrichment culture temperature	30 ̊C
[Ref.: #65267]	Isolation/enrichment procedure	The hybrid maize seed suspension (about 100 g) was prepared according to the procedure described by Qiu et al. [10]. Strains 5L6 T and 6L6 were isolated on LB agar which had been inoculated with a suspension of maize seeds and incubated at 30°C for 48 h.
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Plant	#Fruit (Seed)
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP965576 (>99% sequence identity) for Bacillaceae from Microbeatlas

Aquatic Samples	80
Soil Samples	273
Animal Samples	120
Plant Samples	68
Total Samples	541

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64919]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64919]	Bacillus ciccensis strain 5L6 16S ribosomal RNA gene, partial sequence	KP965576	1512	ENA	1670641 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cytobacillus solani 5L6	GCA_006704205	complete	GenBank	1637975 tax ID	*

[Ref.: #64919]

GC-content

37.4 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cytobacillus solani 5L6 NCBI: GCA_006704205.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cytobacillus solani 5L6 NCBI: GCA_006704205.1
[Ref.: #69480]	motile	yes	90.809	yes
[Ref.: #69480]	flagellated	yes	90.422	yes
[Ref.: #69480]	gram-positive	yes	91.021	yes
[Ref.: #69480]	anaerobic	no	97.875	no
[Ref.: #69480]	aerobic	yes	84.932	no
[Ref.: #69480]	halophile	no	80.174	yes
[Ref.: #69480]	spore-forming	yes	96.358	no
[Ref.: #69480]	thermophile	no	98.809	yes
[Ref.: #69480]	glucose-util	yes	86.746	yes
[Ref.: #69480]	glucose-ferment	no	93.628	no

Availability in culture collections External links

[Ref.: #64919]

Culture collection no.

DSM 104513, CICC 23855, KCTC 33663

[Ref.: #111079]

SI-ID 403524

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus ciccensis sp. nov., isolated from maize (Zea mays L.) seeds.	Liu Y, Li N, Eom MK, Schumann P, Zhang X, Cao Y, Ge Y, Xiao M, Zhao J, Cheng C, Kim SG	Int J Syst Evol Microbiol	10.1099/ijsem.0.002341	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64919

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104513

#65267

Yang Liu, Nannan Li, Mi Kyung Eom, Peter Schumann, Xin Zhang, Yanhua Cao, Yuanyuan Ge, Ming Xiao, Jiuran Zhao, Chi Cheng, Song-Gun Kim: Bacillus ciccensis sp. nov., isolated from maize (Zea mays L.) seeds. IJSEM 67: 4606 - 4611 2017 ( DOI 10.1099/ijsem.0.002341 , PubMed 28984568 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111079

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403524.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Cytobacillus ciccensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF