Name: Gottfriedia solisilvae NEAU-cbsb5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158259

Type strain:

Species: Gottfriedia solisilvae

Strain Designation: NEAU-cbsb5

Culture col. no.: DSM 100485, CGMCC 1.14993

Strain history: <- C. Liu, Northeast Agricultural Univ., Harbin, China; NEAU-cbsb5

NCBI tax ID(s): 1516104 (species)

SI-ID 397790

DSM 100485

Section

Gottfriedia solisilvae NEAU-cbsb5 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and builds singly or occasionally in pairs.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Gottfriedia*
	Species	**Gottfriedia solisilvae**
	Full Scientific Name (LPSN)	Gottfriedia solisilvae (Pan et al. 2017) Gupta et al. 2020

Synonym

Bacillus solisilvae

Information on morphological and physiological properties Morphology

[Ref.: #65400]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65400]	Cell length	1.8-2.1 µm

[Ref.: #65400]	Cell width	0.5-0.7 µm

[Ref.: #65400]	Cell shape	rod-shaped

[Ref.: #65400]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.857

[Ref.: #65400]	Flagellum arrangement	peritrichous

[Ref.: #65400]	Colony size	2.5-3.3 mm
[Ref.: #65400]	Colony color	cream
[Ref.: #65400]	Colony shape	circular
[Ref.: #65400]	Incubation period	2 days
[Ref.: #65400]	Cultivation medium used	nutrient agar

[Ref.: #65400]	Multicellular complex forming ability	yes
[Ref.: #65400]	Multicellular complex name	singly or occasionally in pairs

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64918]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #64918]

Culture medium growth

[Ref.: #64918]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64918]

Culture medium composition

expand / minimize

[Ref.: #65400]

Culture medium

Nutrient agar (NA)

[Ref.: #65400]

Culture medium growth

		Temperature
[Ref.: #64918]	growth	30 °C
[Ref.: #65400]	growth	15-40 °C
[Ref.: #65400]	optimum	30 °C

[Ref.: #64918]	Temperature range	mesophilic
[Ref.: #65400]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65400]			optimum	7.0
[Ref.: #65400]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65400]

Oxygen tolerance

facultative anaerobe

[Ref.: #65400]	Spore description	oval and sub-terminal and occur in slightly swollen sporangia after 2 days of incubation at 30C on NA containing MnSO4 (5mg/l 1)
[Ref.: #65400]	Type of spore	endospore
[Ref.: #65400]	Ability of spore formation	yes
[Ref.: #65400]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65400]		NaCl		growth	4.0	%(w/v)

[Ref.: #65400]	Observation	moist and loosely butyrous
[Ref.: #65400]	Observation	major menaquinones are MK-7 and MK-6
[Ref.: #65400]	Observation	cell-wall peptidoglycan contains meso-DAP as the diagnostic diamino acid

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65400]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #65400]	amygdalin ChEBI	-	builds acid from
[Ref.: #65400]	arbutin ChEBI	+	builds acid from
[Ref.: #65400]	cellobiose ChEBI	+	builds acid from
[Ref.: #65400]	creatine ChEBI	-	carbon source
[Ref.: #65400]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65400]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65400]	D-fructose ChEBI	-	carbon source
[Ref.: #65400]	D-fructose ChEBI	+	builds acid from
[Ref.: #65400]	D-fucose ChEBI	-	builds acid from
[Ref.: #65400]	D-galactose ChEBI	-	carbon source
[Ref.: #65400]	D-galactose ChEBI	-	builds acid from
[Ref.: #65400]	D-glucose ChEBI	-	carbon source
[Ref.: #65400]	D-glucose ChEBI	+	builds acid from
[Ref.: #65400]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65400]	D-mannitol ChEBI	+	builds acid from
[Ref.: #65400]	D-mannitol ChEBI	+	carbon source
[Ref.: #65400]	D-mannose ChEBI	-	builds acid from
[Ref.: #65400]	D-ribose ChEBI	-	builds acid from
[Ref.: #65400]	D-ribose ChEBI	-	carbon source
[Ref.: #65400]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65400]	D-sorbitol ChEBI	+	carbon source
[Ref.: #65400]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65400]	D-xylose ChEBI	+	carbon source
[Ref.: #65400]	D-xylose ChEBI	-	builds acid from
[Ref.: #65400]	erythritol ChEBI	-	builds acid from
[Ref.: #65400]	esculin ChEBI	+	builds acid from
[Ref.: #65400]	esculin ferric citrate	+	hydrolysis
[Ref.: #65400]	galactitol ChEBI	-	builds acid from
[Ref.: #65400]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65400]	gluconate ChEBI	-	builds acid from
[Ref.: #65400]	glycerol ChEBI	-	builds acid from
[Ref.: #65400]	glycine ChEBI	+	carbon source
[Ref.: #65400]	glycogen ChEBI	+	builds acid from
[Ref.: #65400]	inulin ChEBI	-	builds acid from
[Ref.: #65400]	isocitrate ChEBI	-	assimilation
[Ref.: #65400]	L-alanine ChEBI	-	carbon source
[Ref.: #65400]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65400]	L-arabinose ChEBI	-	carbon source
[Ref.: #65400]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65400]	L-arginine ChEBI	-	carbon source
[Ref.: #65400]	L-asparagine ChEBI	-	carbon source
[Ref.: #65400]	L-aspartate ChEBI	+	carbon source
[Ref.: #65400]	L-fucose ChEBI	-	builds acid from
[Ref.: #65400]	L-glutamate ChEBI	+	carbon source
[Ref.: #65400]	L-glutamine ChEBI	-	carbon source
[Ref.: #65400]	L-proline ChEBI	+	carbon source
[Ref.: #65400]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65400]	L-rhamnose ChEBI	+	carbon source
[Ref.: #65400]	L-serine ChEBI	-	carbon source
[Ref.: #65400]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65400]	L-threonine ChEBI	-	carbon source
[Ref.: #65400]	L-tyrosine ChEBI	+	carbon source
[Ref.: #65400]	L-xylose ChEBI	-	builds acid from
[Ref.: #65400]	lactose ChEBI	-	builds acid from
[Ref.: #65400]	lactose ChEBI	-	carbon source
[Ref.: #65400]	malate ChEBI	+	carbon source
[Ref.: #65400]	maltose ChEBI	-	carbon source
[Ref.: #65400]	maltose ChEBI	+	builds acid from
[Ref.: #65400]	mannose ChEBI	-	carbon source
[Ref.: #65400]	melezitose ChEBI	-	builds acid from
[Ref.: #65400]	melibiose ChEBI	-	builds acid from
[Ref.: #65400]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65400]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65400]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65400]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65400]	myo-inositol ChEBI	-	carbon source
[Ref.: #65400]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65400]	nitrate ChEBI	-	reduction
[Ref.: #65400]	oxalate ChEBI	+	carbon source
[Ref.: #65400]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65400]	pyruvate ChEBI	-	carbon source
[Ref.: #65400]	raffinose ChEBI	-	builds acid from
[Ref.: #65400]	raffinose ChEBI	+	carbon source
[Ref.: #65400]	ribitol ChEBI	-	builds acid from
[Ref.: #65400]	salicin ChEBI	+	builds acid from
[Ref.: #65400]	starch ChEBI	+	builds acid from
[Ref.: #65400]	starch ChEBI	+	hydrolysis
[Ref.: #65400]	sucrose ChEBI	-	builds acid from
[Ref.: #65400]	sucrose ChEBI	+	carbon source
[Ref.: #65400]	trehalose ChEBI	+	builds acid from
[Ref.: #65400]	turanose ChEBI	-	builds acid from
[Ref.: #65400]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65400]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65400]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65400]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65400]	casein	no
[Ref.: #65400]	cellulose ChEBI	no
[Ref.: #65400]	gelatin ChEBI	no
[Ref.: #65400]	indole ChEBI	no
[Ref.: #65400]	sulfide ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #65400]		acetoin ChEBI	-
[Ref.: #65400]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #65400]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65400]	catalase	+	1.11.1.6
[Ref.: #65400]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65400]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65400]	tryptophan deaminase	-	4.1.99.1
[Ref.: #65400]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64918]	Sample type/isolated from	forest soil
[Ref.: #64918]	Geographic location (country and/or sea, region)	Heilongjiang Province (42° 06' N 128° 04' E), Changbai Mountain at an altitude of 1500 m)
[Ref.: #64918]	Country	China
[Ref.: #64918]	Country ISO 3 Code	CHN
[Ref.: #64918]	Continent	Asia
[Ref.: #64918]	Geographic location	42.1000°/128.0670°

[Ref.: #65400]	Sample type/isolated from	forest soil
[Ref.: #65400]	Geographic location (country and/or sea, region)	Changbai Mountain, Heilongjiang Province
[Ref.: #65400]	Country	China
[Ref.: #65400]	Country ISO 3 Code	CHN
[Ref.: #65400]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ733017 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	505
Soil Samples	3503
Animal Samples	669
Plant Samples	391
Total Samples	5068

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64918]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64918]	Bacillus solisilvae strain NEAU-cbsb5 16S ribosomal RNA gene, partial sequence	KJ733017	1543	ENA	1516104 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gottfriedia solisilvae NEAU-cbsb5	GCA_002128405	contig	GenBank	1516104 tax ID	*
[Ref.: #66792]	Gottfriedia solisilvae CGMCC 1.14993	GCA_014640495	scaffold	GenBank	1516104 tax ID	*
[Ref.: #66792]	Bacillus solisilvae strain CGMCC 1.14993	1516104.166	wgs	PATRIC	1516104 tax ID	*
[Ref.: #66792]	Bacillus sp. NEAU-cbsb5 strain NEAU-cbsb5	1516104.3	wgs	PATRIC	1516104 tax ID	*

[Ref.: #64918]	GC-content	33.0 mol%	sequence analysis
[Ref.: #65400]	GC-content	33 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus sp. NEAU-cbsb5 strain NEAU-cbsb5 PATRIC: 1516104.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Gottfriedia solisilvae CGMCC 1.14993 NCBI: GCA_014640495.1
[Ref.: #69480]	flagellated	yes	86.592	yes
[Ref.: #69480]	gram-positive	yes	87.184	no
[Ref.: #69480]	anaerobic	no	98.975	yes
[Ref.: #69480]	aerobic	yes	73.359	yes
[Ref.: #69480]	halophile	no	90.951	no
[Ref.: #69480]	spore-forming	yes	96.6	yes
[Ref.: #69480]	glucose-util	yes	84.427	yes
[Ref.: #69480]	thermophile	no	98.093	yes
[Ref.: #69480]	motile	yes	89.536	yes
[Ref.: #69480]	glucose-ferment	no	89.464	no

Availability in culture collections External links

[Ref.: #64918]

Culture collection no.

DSM 100485, CGMCC 1.14993

[Ref.: #111078]

SI-ID 397790

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus solisilvae sp. nov., isolated from forest soil.	Pan T, He H, Wang X, Shen Y, Zhao J, Yan K, Wang X, Liu C, Zhang J, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.002312	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64918

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100485

#65400

Tong Pan, Hairong He, Xiaochong Wang, Yibo Shen, Junwei Zhao, Kai Yan, Xiangjing Wang, Chongxi Liu, Ji Zhang, Wensheng Xiang: Bacillus solisilvae sp. nov., isolated from forest soil. IJSEM 67: 4449 - 4455 2017 ( DOI 10.1099/ijsem.0.002312 , PubMed 28920837 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111078

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397790.1 )

* These data were automatically processed and therefore are not curated

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Bacillus sp. NEAU-cbsb5 strain NEAU-cbsb5

Gottfriedia solisilvae CGMCC 1.14993

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