Name: Streptomyces roietensis WES2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158258

Type strain:

Species: Streptomyces roietensis

Strain Designation: WES2

Culture col. no.: DSM 101729, NRRL B-65344

Strain history: <- O. Kaewkla, Flinders Univ., Department of Medical Biotechnology, Adeleide, South Australia; WES2

NCBI tax ID(s): 2044847 (species)

SI-ID 404496

DSM 101729

Section

Streptomyces roietensis WES2 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate mycelium and was isolated from surface-sterilized stem of jasmine rice Oryza sativa KDML 105.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces roietensis**
	Full Scientific Name (LPSN)	Streptomyces roietensis Kaewkla and Franco 2017

Information on morphological and physiological properties Morphology

[Ref.: #65253]	Gram stain	positive

[Ref.: #65253]	Pigment production	yes
[Ref.: #65253]	Pigment name	Melanin

[Ref.: #65253]	Multicellular complex forming ability	yes
[Ref.: #65253]	Multicellular complex name	substrate mycelium
[Ref.: #65253]	Multicellular complex color	light to dark brown

[Ref.: #65253]	Multicellular complex forming ability	yes
[Ref.: #65253]	Multicellular complex name	aerial mycelia
[Ref.: #65253]	Multicellular complex color	greenish grey or cream
[Ref.: #65253]	Multicellular complex further description	The mycelium (0.5 µm diameter) is extensively branched and forms chains of looped spores

Multimedia content

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Caption:

Medium 65 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

ISP2

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Culture medium growth

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Culture medium

ISP3

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Culture medium growth

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Culture medium

ISP4

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Culture medium growth

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Culture medium

ISP5

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Culture medium growth

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Culture medium

ISP7

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Culture medium growth

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Culture medium

Bennett's agar

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Culture medium growth

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Culture medium

half-strength potato dextrose agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #64917]	growth	28 °C
[Ref.: #65253]	growth	15-45 °C
[Ref.: #65253]	optimum	27-37 °C

[Ref.: #64917]	Temperature range	mesophilic
[Ref.: #65253]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65253]			optimum	6-9
[Ref.: #65253]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #65253]	Spore description	A rod-shaped spore (0.8 µm diameter x 1.1 µm length)
[Ref.: #65253]	Ability of spore formation	yes

	Salt	Tested relation		Salt conc.
[Ref.: #65253]	NaCl		growth	5	%(w/v)
[Ref.: #65253]	NaCl		growth	1	%(w/v)
[Ref.: #65253]	NaCl		growth	3	%(w/v)
[Ref.: #65253]	NaCl	no	growth	10	%(w/v)
[Ref.: #65253]	NaCl	no	growth	15	%(w/v)
[Ref.: #65253]	NaCl	no	growth	20	%(w/v)

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Observation

strongly inhibit bacterial leaf blight; X. oryzae KHWK 4.1_UBN_06 in vitro.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65253]	(S)-lactate ChEBI	+	assimilation
[Ref.: #65253]	acetate ChEBI	+	assimilation
[Ref.: #65253]	benzene ChEBI	+	assimilation
[Ref.: #65253]	benzoate ChEBI	-	assimilation
[Ref.: #65253]	cellulose ChEBI	+	builds acid from
[Ref.: #65253]	citrate ChEBI	+	assimilation
[Ref.: #65253]	erythritol ChEBI	-	builds acid from
[Ref.: #65253]	esculin ChEBI	+	hydrolysis
[Ref.: #65253]	fructose ChEBI	+	builds acid from
[Ref.: #65253]	fucose ChEBI	+	builds acid from
[Ref.: #65253]	galactose ChEBI	+	builds acid from
[Ref.: #65253]	glucose ChEBI	+	builds acid from
[Ref.: #65253]	hippurate ChEBI	-	hydrolysis
[Ref.: #65253]	L-arabinose ChEBI	+	builds acid from
[Ref.: #65253]	L-tartrate ChEBI	-	assimilation
[Ref.: #65253]	L-tyrosine ChEBI	+	hydrolysis
[Ref.: #65253]	malate ChEBI	+	assimilation
[Ref.: #65253]	maltose ChEBI	+	builds acid from
[Ref.: #65253]	mannitol ChEBI	+	builds acid from
[Ref.: #65253]	mannose ChEBI	+	builds acid from
[Ref.: #65253]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #65253]	myo-inositol ChEBI	+	builds acid from
[Ref.: #65253]	phenol ChEBI	-	carbon source
[Ref.: #65253]	propionate ChEBI	+	assimilation
[Ref.: #65253]	pyridine ChEBI	+	assimilation
[Ref.: #65253]	raffinose ChEBI	+	builds acid from
[Ref.: #65253]	ribose ChEBI	+	builds acid from
[Ref.: #65253]	skimmed milk	+	hydrolysis
[Ref.: #65253]	sorbitol ChEBI	-	builds acid from
[Ref.: #65253]	starch ChEBI	+	hydrolysis
[Ref.: #65253]	sucrose ChEBI	+	builds acid from
[Ref.: #65253]	toluene ChEBI	+	assimilation
[Ref.: #65253]	trehalose ChEBI	+	builds acid from
[Ref.: #65253]	urea ChEBI	-	hydrolysis
[Ref.: #65253]	uric acid ChEBI	-	hydrolysis
[Ref.: #65253]	xylose ChEBI	+	builds acid from
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	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65253]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64917]	Sample type/isolated from	surface-sterilized stem of jasmine rice Oryza sativa KDML 105
[Ref.: #64917]	Geographic location (country and/or sea, region)	Roi Et Province, Surin Rice Research Centre (16° 3' N, 103° 39' E)
[Ref.: #64917]	Country	Thailand
[Ref.: #64917]	Country ISO 3 Code	THA
[Ref.: #64917]	Continent	Asia
[Ref.: #64917]	Geographic location	16.0500°/103.6500°

[Ref.: #65253]	Sample type/isolated from	stem sample of a jasmine rice plant (Oryza sativa KDML 105)
[Ref.: #65253]	Sampling date	2012
[Ref.: #65253]	Geographic location (country and/or sea, region)	Thung Kura Rong Hai paddy field, Roi Et Province
[Ref.: #65253]	Country	Thailand
[Ref.: #65253]	Country ISO 3 Code	THA
[Ref.: #65253]	Continent	Asia
[Ref.: #65253]	Enrichment culture	humic acid-vitamin agar
[Ref.: #65253]	Enrichment culture duration	2 weeks
[Ref.: #65253]	Enrichment culture temperature	37 ̊C
[Ref.: #65253]	Isolation/enrichment procedure	Crushed surface-sterilized stem tissue was placed onto HVA medium. WES2T emerged as a small colony from the stem tissue after incubation for 2 weeks at 37°C.
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Stem (Branch)
#Engineered	#Treatment	#Sterilized (Desinfected)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64917]	Streptomyces roietensis strain WES2 16S ribosomal RNA gene, partial sequence	KX394336	1434	ENA	2044847 tax ID

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GC-content

70.7 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

DSM 101729, NRRL B-65344

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SI-ID 404496

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces roietensis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of jasmine rice, Oryza sativa KDML 105.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijsem.0.002402	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64917

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101729

#65253

Onuma Kaewkla, Christopher Milton Mathew Franco: Streptomyces roietensis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of jasmine rice, Oryza sativa KDML 105. IJSEM 67: 4868 - 4872 2017 ( DOI 10.1099/ijsem.0.002402 , PubMed 28984216 )

#111077

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404496.1 )

* These data were automatically processed and therefore are not curated

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