Streptomyces resistomycificus (DSM 40133, ATCC 19804, CBS 556.68)

Name: Streptomyces resistomycificus (DSM 40133, ATCC 19804, CBS 556.68)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 15513

Type strain

Strain Designation Pürk 262

Culture col. no. DSM 40133 ATCC 19804 CBS 556.68 ETH 23893 ETH 32680 13 more

NCBI tax ID(s) 67356

Special Collections DSMZ JCM Wink compendium

Links

Streptomyces resistomycificus Pürk 262 is a mesophilic prokaryote that builds an aerial mycelium and produces antibiotic compounds.

antibiotic compound production mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 45638

PCM 2296,ATCC 19804,IFO 12814,NRRL 2290,DSM 40133,JCM 4409,NRRL B-2290,CCRC 13755,NCIMB 9843,VKM Ac-1895,CBS 556.68,NBRC 12814,BCRC 13755,CGMCC 4.1304

@ref 20215

Last LPSN update 2025-12-16 09:50:37

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces resistomycificus

Full scientific name Streptomyces resistomycificus Lindenbein 1952 (Approved Lists 1980)

BacDive ID	Other strains from **Streptomyces resistomycificus** (1)	Type strain
15514	S. resistomycificus MP-2, DSM 40911

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99

Colony morphology

@ref	Colony color	Incubation period	Medium used
21381	Olive brown	10-14 days	ISP 2
21381	Nut brown	10-14 days	ISP 3
21381	Fawn brown	10-14 days	ISP 4
21381	Grey brown	10-14 days	ISP 5
21381	Black brown	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
21381	Aerial Mycelium	Dusty grey	ISP 4
21381
21381			Suter without tyrosine
21381			ISP 2
21381	Aerial Mycelium	Signal grey	ISP 3
21381	Aerial Mycelium	Fawn brown	ISP 4
21381	Aerial Mycelium		ISP 5
21381			ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9335	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21381	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21381	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21381	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21381	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21381	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
9335	positive	growth	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	90.762
125439	obligate aerobe	99.1

Spore formation

@ref	Spore formation	Confidence
125438		91.64
125439		94.5

Compound production

@ref	Compound
9335	resistomycin
20216	Resistomycin = Croceomycin

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
21381	22599 ChEBI	arabinose	+
21381	62968 ChEBI	cellulose	-
21381	28757 ChEBI	fructose	+
21381	17234 ChEBI	glucose	+
21381	37684 ChEBI	mannose	+
21381	17268 ChEBI	myo-inositol	+
21381	16634 ChEBI	raffinose	+
21381	26546 ChEBI	rhamnose	+
21381	17992 ChEBI	sucrose	-
21381	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite	Production
67770	29671 ChEBI	resistomycin

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	+	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
21381	not determinedn.d.	+	-	-	-	+	+	-	+	-	+	-	-	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
9335	soil
67770	Soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9335	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM151426v1 assembly for Streptomyces resistomycificus DSM 40133	scaffold	GCA_001514265	67356.6	2806310739	67356	66.74
67770	ASM127050v1 assembly for Streptomyces resistomycificus NRRL 2290	contig	GCA_001270505	67356.5	2772190671	67356	30.16
67770	ASM71621v1 assembly for Streptomyces resistomycificus NRRL ISP-5133	scaffold	GCA_000716215	67356.3	2806310730	67356

Genome browser: GCA_001514265

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces resistomycificus partial 16S rRNA gene, type strain DSM 40133	AJ310926	1448	67356
20218	Streptomyces resistomycificus partial 16S rRNA gene, strain ISP 5133	AJ399472	1449	67356
20218	Streptomyces resistomycificus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4409	D44124	120	67356
20218	Streptomyces resistomycificus gene for 16S rRNA, partial sequence, strain: NBRC 12814	AB184166	1466	67356

GC content

@ref	GC-content (mol%)	Method
67770	73.6	thermal denaturation, midpoint method (Tm)
67770	71	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Streptomyces resistomycificus NRRL ISP-5133
NCBI: GCA_000716215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	94.50	no
125439	motility	BacteriaNetⓘ	no	89.20	no
125439	gram_stain	BacteriaNetⓘ	positive	99.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces resistomycificus strain DSM 40133
PATRIC: 67356.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.91	no
125438	anaerobic	anaerobicⓘ	no	96.95	no
125438	spore-forming	spore-formingⓘ	yes	91.64	no
125438	aerobic	aerobicⓘ	yes	90.76	no
125438	thermophilic	thermophileⓘ	no	97.00	no
125438	flagellated	motile2+ⓘ	no	87.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates.	Liu XL, Liu SL, Liu M, Kong BH, Liu L, Li YH.	Braz J Microbiol	10.1590/s1517-83822014000100022	2014
Metabolism	Biosynthesis of C-nucleoside antibiotics in actinobacteria: recent advances and future developments.	Zhang M, Kong L, Gong R, Iorio M, Donadio S, Deng Z, Sosio M, Chen W.	Microb Cell Fact	10.1186/s12934-021-01722-z	2022
Metabolism	Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction of C-Nucleoside Scaffolds.	Zhang M, Zhang P, Xu G, Zhou W, Gao Y, Gong R, Cai YS, Cong H, Deng Z, Price NPJ, Mao X, Chen W.	Appl Environ Microbiol	10.1128/aem.01971-19	2020
	Antibiotic heliomycin and its water-soluble 4-aminomethylated derivative provoke cell death in T24 bladder cancer cells by targeting sirtuin 1 (SIRT1).	Lin MH, Islam A, Liu YH, Weng CW, Zhan JH, Liang RH, Tikhomirov AS, Shchekotikhin AE, Chueh PJ.	Am J Cancer Res		2022
	Water-soluble 4-(dimethylaminomethyl)heliomycin exerts greater antitumor effects than parental heliomycin by targeting the tNOX-SIRT1 axis and apoptosis in oral cancer cells.	Islam A, Chang YC, Chen XC, Weng CW, Chen CY, Wang CW, Chen MK, Tikhomirov AS, Shchekotikhin AE, Chueh PJ.	Elife	10.7554/elife.87873	2024
	Resistomycin Suppresses Prostate Cancer Cell Growth by Instigating Oxidative Stress, Mitochondrial Apoptosis, and Cell Cycle Arrest.	Aloufi AS, Habotta OA, Abdelfattah MS, Habib MN, Omran MM, Ali SA, Abdel Moneim AE, Korany SM, Alrajhi AM.	Molecules	10.3390/molecules28237871	2023
Enzymology	The polyketide cyclase RemF from Streptomyces resistomycificus contains an unusual octahedral zinc binding site.	Silvennoinen L, Sandalova T, Schneider G.	FEBS Lett	10.1016/j.febslet.2009.07.061	2009
	Orchestration of discoid polyketide cyclization in the resistomycin pathway.	Fritzsche K, Ishida K, Hertweck C.	J Am Chem Soc	10.1021/ja800251m	2008
Metabolism	A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; exploring polyketide cyclization beyond linear and angucyclic patterns.	Jakobi K, Hertweck C.	J Am Chem Soc	10.1021/ja0390698	2004
	The boat-shaped polyketide resistoflavin results from re-facial central hydroxylation of the discoid metabolite resistomycin.	Ishida K, Maksimenka K, Fritzsche K, Scherlach K, Bringmann G, Hertweck C.	J Am Chem Soc	10.1021/ja064550u	2006
	Overproduction, crystallization and X-ray diffraction data analysis of ectoine synthase from the cold-adapted marine bacterium Sphingopyxis alaskensis.	Kobus S, Widderich N, Hoeppner A, Bremer E, Smits SH.	Acta Crystallogr F Struct Biol Commun	10.1107/s2053230x15011115	2015
Enzymology	FR901277, a novel inhibitor of human leukocyte elastase from Streptomyces resistomycificus. Producing organism, fermentation, isolation, physico-chemical and biological properties.	Fujie K, Shinguh Y, Hatanaka H, Shigematsu N, Murai H, Fujita T, Yamashita M, Okamoto M, Okuhara M.	J Antibiot (Tokyo)	10.7164/antibiotics.46.908	1993
	The Hexahistidine Motif of Host-Defense Protein Human Calprotectin Contributes to Zinc Withholding and Its Functional Versatility.	Nakashige TG, Stephan JR, Cunden LS, Brophy MB, Wommack AJ, Keegan BC, Shearer JM, Nolan EM.	J Am Chem Soc	10.1021/jacs.6b06845	2016
Metabolism	Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli.	Cummings M, Peters AD, Whitehead GFS, Menon BRK, Micklefield J, Webb SJ, Takano E.	PLoS Biol	10.1371/journal.pbio.3000347	2019
Metabolism	Phenalenone Polyketide Cyclization Catalyzed by Fungal Polyketide Synthase and Flavin-Dependent Monooxygenase.	Gao SS, Duan A, Xu W, Yu P, Hang L, Houk KN, Tang Y.	J Am Chem Soc	10.1021/jacs.6b01528	2016
Metabolism	Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from Streptomyces albus JA3453.	Zhao C, Ju J, Christenson SD, Smith WC, Song D, Zhou X, Shen B, Deng Z.	J Bacteriol	10.1128/jb.00173-06	2006
Enzymology	Biochemistry and Crystal Structure of Ectoine Synthase: A Metal-Containing Member of the Cupin Superfamily.	Widderich N, Kobus S, Hoppner A, Riclea R, Seubert A, Dickschat JS, Heider J, Smits SH, Bremer E.	PLoS One	10.1371/journal.pone.0151285	2016
Metabolism	Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units.	Zhao C, Coughlin JM, Ju J, Zhu D, Wendt-Pienkowski E, Zhou X, Wang Z, Shen B, Deng Z.	J Biol Chem	10.1074/jbc.m109.090092	2010
Pathogenicity	Calcium ion gradients modulate the zinc affinity and antibacterial activity of human calprotectin.	Brophy MB, Hayden JA, Nolan EM.	J Am Chem Soc	10.1021/ja307974e	2012
Metabolism	Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis.	Van Lanen SG, Oh TJ, Liu W, Wendt-Pienkowski E, Shen B.	J Am Chem Soc	10.1021/ja073275o	2007
Metabolism	Novel pathway of salicylate degradation by Streptomyces sp. strain WA46.	Ishiyama D, Vujaklija D, Davies J.	Appl Environ Microbiol	10.1128/aem.70.3.1297-1306.2004	2004
Metabolism	Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics.	Gomes ES, Schuch V, de Macedo Lemos EG.	Braz J Microbiol	10.1590/s1517-83822013000400002	2013
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Metabolism	Streptomyces avermitilis sp. nov., nom. rev., a taxonomic home for the avermectin-producing streptomycetes.	Kim SB, Goodfellow M.	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2011	2002
Phylogeny	Streptomyces ziwulingensis sp. nov., isolated from grassland soil.	Lin YB, Wang XY, Wang TT, An SS, Shi P, Wei GH	Int J Syst Evol Microbiol	10.1099/ijs.0.043026-0	2012
Phylogeny	Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil.	Lee HJ, Whang KS	Int J Syst Evol Microbiol	10.1099/ijsem.0.001236	2016
Phylogeny	Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China.	Guo JK, Lin YB, Zhao ML, Sun R, Wang TT, Tang M, Wei GH	Int J Syst Evol Microbiol	10.1099/ijs.0.004713-0	2009
Phylogeny	Streptomyces roseoalbus sp. nov., an actinomycete isolated from soil in Yunnan, China.	Xu LH, Jiang Y, Li WJ, Wen ML, Li MG, Jiang CL	Antonie Van Leeuwenhoek	10.1007/s10482-004-3720-y	2005

References

#9335	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40133
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#21381	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces resistomycificus (DSM 40133, ATCC 19804, CBS 556.68)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001514265

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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