Strain identifier
BacDive ID: 15513
Type strain: 
Species: Streptomyces resistomycificus
Strain Designation: Pürk 262
Strain history: KCC S-0409 <-- IFO 12814 <-- SAJ <-- ISP 5133 <-- P. Wilde Pürk 262.
NCBI tax ID(s): 67356 (species)
version 8.1 (current version)
General
@ref: 9335
BacDive-ID: 15513
DSM-Number: 40133
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production
description: Streptomyces resistomycificus Pürk 262 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.
NCBI tax id
- NCBI tax id: 67356
- Matching level: species
strain history
| @ref | history |
|---|---|
| 9335 | <- E.B. Shirling, ISP <- P. Wilde, Pürk 262 |
| 67770 | KCC S-0409 <-- IFO 12814 <-- SAJ <-- ISP 5133 <-- P. Wilde Pürk 262. |
doi: 10.13145/bacdive15513.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces resistomycificus
- full scientific name: Streptomyces resistomycificus Lindenbein 1952 (Approved Lists 1980)
@ref: 9335
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces resistomycificus
full scientific name: Streptomyces resistomycificus Lindenbein 1952 emend. Nouioui et al. 2018
strain designation: Pürk 262
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 93.474 | |
| 69480 | 100 | positive |
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 21381 | Olive brown | 10-14 days | ISP 2 |
| 21381 | Nut brown | 10-14 days | ISP 3 |
| 21381 | Fawn brown | 10-14 days | ISP 4 |
| 21381 | Grey brown | 10-14 days | ISP 5 |
| 21381 | Black brown | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 21381 | yes | Aerial Mycelium | Dusty grey | ISP 4 |
| 21381 | no | |||
| 21381 | no | Suter without tyrosine | ||
| 21381 | no | ISP 2 | ||
| 21381 | yes | Aerial Mycelium | Signal grey | ISP 3 |
| 21381 | yes | Aerial Mycelium | Fawn brown | ISP 4 |
| 21381 | yes | Aerial Mycelium | ISP 5 | |
| 21381 | no | ISP 7 |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9335 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 21381 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 21381 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 21381 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 21381 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 21381 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 9335 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 98 |
| 69480 | yes | 100 |
compound production
| @ref | compound |
|---|---|
| 9335 | resistomycin |
| 20216 | Resistomycin = Croceomycin |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity |
|---|---|---|---|
| 21381 | 17234 | glucose | + |
| 21381 | 22599 | arabinose | + |
| 21381 | 17992 | sucrose | - |
| 21381 | 18222 | xylose | + |
| 21381 | 17268 | myo-inositol | + |
| 21381 | 37684 | mannose | + |
| 21381 | 28757 | fructose | + |
| 21381 | 26546 | rhamnose | + |
| 21381 | 16634 | raffinose | + |
| 21381 | 62968 | cellulose | - |
metabolite production
- @ref: 67770
- Chebi-ID: 29671
- metabolite: resistomycin
- production: yes
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | - | |
| 68382 | esterase lipase (C 8) | - | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | + | |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | trypsin | + | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21381 | + | - | - | - | + | + | - | + | - | + | - | - | + | - | + | + | + | - | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type |
|---|---|
| 9335 | soil |
| 67770 | Soil |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 9335
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Streptomyces resistomycificus partial 16S rRNA gene, type strain DSM 40133 | AJ310926 | 1448 | ena | 67356 |
| 20218 | Streptomyces resistomycificus partial 16S rRNA gene, strain ISP 5133 | AJ399472 | 1449 | ena | 67356 |
| 20218 | Streptomyces resistomycificus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4409 | D44124 | 120 | ena | 67356 |
| 20218 | Streptomyces resistomycificus gene for 16S rRNA, partial sequence, strain: NBRC 12814 | AB184166 | 1466 | ena | 67356 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Streptomyces resistomycificus strain DSM 40133 | 67356.6 | wgs | patric | 67356 |
| 66792 | Streptomyces resistomycificus strain NRRL 2290 | 67356.5 | wgs | patric | 67356 |
| 66792 | Streptomyces resistomycificus DSM 40133 | 2806310739 | draft | img | 67356 |
| 66792 | Streptomyces resistomycificus NRRL 2290 | 2772190671 | draft | img | 67356 |
| 67770 | Streptomyces resistomycificus NRRL ISP-5133 | GCA_000716215 | scaffold | ncbi | 67356 |
| 67770 | Streptomyces resistomycificus NRRL 2290 | GCA_001270505 | contig | ncbi | 67356 |
| 67770 | Streptomyces resistomycificus DSM 40133 | GCA_001514265 | scaffold | ncbi | 67356 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 67770 | 73.6 | thermal denaturation, midpoint method (Tm) |
| 67770 | 71 | genome sequence analysis |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 98 | no |
| gram-positive | yes | 89.681 | no |
| anaerobic | no | 99.152 | no |
| halophile | no | 92.536 | no |
| spore-forming | yes | 95.399 | no |
| glucose-util | yes | 90.945 | no |
| thermophile | no | 98.584 | yes |
| flagellated | no | 97.643 | no |
| motile | no | 94.144 | no |
| aerobic | yes | 91.597 | no |
| glucose-ferment | no | 89.174 | no |
External links
@ref: 9335
culture collection no.: DSM 40133, ATCC 19804, CBS 556.68, ETH 23893, ETH 32680, IFO 12814, IMRU 3658, ISP 5133, JCM 4409, NBRC 12814, NRRL 2290, RIA 1083, BCRC 13755, NCIMB 9843, NRRL ISP-5133, PCM 2296, VKM Ac-1895
straininfo link
- @ref: 84599
- straininfo: 45638
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15803387 | Streptomyces roseoalbus sp. nov., an actinomycete isolated from soil in Yunnan, China. | Xu LH, Jiang Y, Li WJ, Wen ML, Li MG, Jiang CL | Antonie Van Leeuwenhoek | 10.1007/s10482-004-3720-y | 2005 | Bacterial Typing Techniques, Base Sequence, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Molecular Sequence Data, Phospholipids/analysis/isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification, Vitamin K 2/analysis/isolation & purification | Enzymology |
| Phylogeny | 19502310 | Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China. | Guo JK, Lin YB, Zhao ML, Sun R, Wang TT, Tang M, Wei GH | Int J Syst Evol Microbiol | 10.1099/ijs.0.004713-0 | 2009 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Drug Resistance, Bacterial, Genotype, Lead/*pharmacology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants/*pharmacology, Streptomyces/*classification/drug effects/genetics/isolation & purification | Genetics |
| Phylogeny | 22888189 | Streptomyces ziwulingensis sp. nov., isolated from grassland soil. | Lin YB, Wang XY, Wang TT, An SS, Shi P, Wei GH | Int J Syst Evol Microbiol | 10.1099/ijs.0.043026-0 | 2012 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Poaceae, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analysis | Genetics |
| Phylogeny | 27298302 | Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil. | Lee HJ, Whang KS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001236 | 2016 | Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sasa/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 9335 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40133) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40133 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20216 | Curators of the HKI | http://www.leibniz-hki.de/de/ | Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 21381 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40133.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68382 | Automatically annotated from API zym | |||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 84599 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45638.1 | StrainInfo: A central database for resolving microbial strain identifiers |