Name: Cuniculiplasma divulgatum S5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141129

Type strain:

Species: Cuniculiplasma divulgatum

Strain Designation: S5

Culture col. no.: JCM 30642, VKM B-2941

Strain history: O. Golyshina; Bangor Univ., UK; S5.

NCBI tax ID(s): 1673428 (species)

Other strains from Cuniculiplasma divulgatum

C. divulgatum JCM 30641, VKM B-2940

Section

Cuniculiplasma divulgatum S5 is a facultative anaerobe, organoheterotroph, spore-forming archaeon that was isolated from acidic streamer formed on the surfaces of copper-ore-containing suldic deposits.

pleomorphic-shaped
spore-forming
facultative anaerobe
organoheterotroph
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Thermoplasmata*
	Order	*Thermoplasmatales*
	Family	*Cuniculiplasmataceae*
	Genus	*Cuniculiplasma*
	Species	**Cuniculiplasma divulgatum**
	Full Scientific Name (LPSN)	Cuniculiplasma divulgatum Golyshina et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.968

[Ref.: #44041]	Cell shape	pleomorphic-shaped

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44041]

Culture medium

modified medium 88 (DSMZ)

[Ref.: #44041]

Culture medium growth

[Ref.: #44041]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #44041]	growth	10-48 °C
[Ref.: #44041]	optimum	37-40 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #67770]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #44041]			optimum	1.0-1.2
[Ref.: #44041]			growth	0.5-4.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44041]

Oxygen tolerance

facultative anaerobe

[Ref.: #44041]

Nutrition type

organoheterotroph

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995

[Ref.: #44041]	Observation	cell wall
[Ref.: #67770]	Observation	quinones: Menaquinones and thermoplasmaquinones

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44041]	Sample type/isolated from	acidic streamer formed on the surfaces of copper-ore-containing suldic deposits
[Ref.: #44041]	Geographic location (country and/or sea, region)	Cantareras Mine
[Ref.: #44041]	Country	Spain
[Ref.: #44041]	Country ISO 3 Code	ESP
[Ref.: #44041]	Continent	Europe
[Ref.: #44041]	Enrichment culture	modified medium 88 (DSMZ)
[Ref.: #44041]	Enrichment culture composition	All g/l (NH 4)2SO4, 1.3; KH2PO4, 0.28; MgSO4 . 0.7 H2O, 0.25; CaCl2 . 2 H2O, 0.07; FeCl3 . 6 H2O, 0.02. Other salts in medium 88 were replaced with the trace element solution SL-10 from DSMZ medium 320 at a concentration of 1 : 1000. Beef extract (3 g/l), 0.06 % betaine and Kao and Michayluk’s vitamin solution (Sigma-Aldrich) at 1 : 100 (v/v) were added to the medium. The medium was adjusted to pH 1.0–1.2 with concentrated H2SO4.
[Ref.: #44041]	Enrichment culture duration	5 days
[Ref.: #44041]	Enrichment culture temperature	37 ̊C

[Ref.: #67770]	Sample type/isolated from	Acidic water from the surface of a copper mine site in Mynydd Parys
[Ref.: #67770]	Geographic location (country and/or sea, region)	Anglesey
[Ref.: #67770]	Country	United Kingdom
[Ref.: #67770]	Country ISO 3 Code	GBR
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Condition	#Acidic	-
#Engineered	#Other	#Mine
#Environmental	#Microbial community	-
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KT005320 (>99% sequence identity) for Cuniculiplasma divulgatum subclade from Microbeatlas

Aquatic Samples	135
Soil Samples	5
Animal Samples	2
Plant Samples
Total Samples	142

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #44041]	Cuniculiplasma divulgatum strain S5 16S ribosomal RNA gene, partial sequence	KT005321	1469	GenBank	1673428 tax ID
[Ref.: #67770]	Cuniculiplasma divulgatum strain PM4 16S ribosomal RNA gene, partial sequence	KT005320	1469	ENA	1673428 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cuniculiplasma divulgatum S5(T) (=JCM 30642; =VKM B-2941)	GCA_900083515	complete	GenBank	1673428 tax ID	*
[Ref.: #66792]	Cuniculiplasma divulgatum S5	2773858060	complete	JGI IMG/M	1673428 tax ID	*

[Ref.: #67770]

GC-content

37.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cuniculiplasma divulgatum S5(T) (=JCM 30642 NCBI: GCA_900083515.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cuniculiplasma divulgatum S5(T) (=JCM 30642 NCBI: GCA_900083515.1
[Ref.: #69480]	motile	no	86.612	no
[Ref.: #69480]	flagellated	no	98.029	no
[Ref.: #69480]	gram-positive	no	90.308	no
[Ref.: #69480]	anaerobic	no	55.507	no
[Ref.: #69480]	aerobic	no	91.531	yes
[Ref.: #69480]	halophile	no	87.066	no
[Ref.: #69480]	spore-forming	no	94.033	no
[Ref.: #69480]	glucose-ferment	no	64.513	no
[Ref.: #69480]	thermophile	yes	95.57	no
[Ref.: #69480]	glucose-util	yes	82.919	no

Availability in culture collections External links

[Ref.: #44041]

Culture collection no.

JCM 30642, VKM B-2941

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	The novel extremely acidophilic, cell-wall-deficient archaeon Cuniculiplasma divulgatum gen. nov., sp. nov. represents a new family, Cuniculiplasmataceae fam. nov., of the order Thermoplasmatales.	Golyshina OV, Lunsdorf H, Kublanov IV, Goldenstein NI, Hinrichs KU, Golyshin PN	Int J Syst Evol Microbiol	10.1099/ijsem.0.000725	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44041

Olga V. Golyshina, Heinrich Lünsdorf, Ilya V. Kublanov, Nadine I. Goldenstein, Kai-Uwe Hinrichs and Peter N. Golyshin: The novel extremely acidophilic, cell-wall-deficient archaeon Cuniculiplasma divulgatum gen. nov., sp. nov. represents a new family, Cuniculiplasmataceae fam. nov., of the order Thermoplasmatales. IJSEM 66: 332 - 340 2016 ( DOI 10.1099/ijsem.0.000725 , PubMed 26518885 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Cuniculiplasma divulgatum S5(T) (=JCM 30642

Cuniculiplasma divulgatum S5(T) (=JCM 30642

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