Quisquiliibacterium transsilvanicum (DSM 29781, JCM 31785, CGI-09)

Name: Quisquiliibacterium transsilvanicum (DSM 29781, JCM 31785, CGI-09)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141111

Type strain

Strain Designation CGI-09

Culture col. no. DSM 29781 JCM 31785 CGI-09

NCBI tax ID(s) 1549638

Special Collections DSMZ JCM Literature strains

History <- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary; CGI-09 <- I. Máthé and R.-D. Fikó, T. Felföldi; Eötvös Loránd Univ., Hungary; CGI-09.

Links

Quisquiliibacterium transsilvanicum CGI-09 is an aerobe, rod-shaped bacterium that forms circular colonies and was isolated from activated sludge bioreactor which treated landfill leachate.

rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 406943

JCM 31785,DSM 29781

@ref 20215

Last LPSN update 2026-02-09 11:33:49

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Quisquiliibacterium

Species Quisquiliibacterium transsilvanicum

Full scientific name Quisquiliibacterium transsilvanicum Felföldi et al. 2017

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Gram stain	Confidence
44019	1.0-1.2 µm	0.6-0.7 µm	rod-shaped
44019				negative
125439				negative	92.9
125438				negative	98

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
44019	1 mm	beige	circular	4-6 days	nutrient medium

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
44019	nutrient medium			DSM medium 1, pH 7
64934	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

Culture temp

@ref	Growth	Type	Temperature (°C)
44019	positive	growth	10-45
44019	positive	optimum	25-30
64934	positive	growth	20
67770	positive	growth	21

Culture pH

@ref	Ability	Type	PH
44019	positive	growth	7.0-8.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
44019	aerobe
125439	obligate aerobe	92.5

Spore formation

@ref	Spore formation	Confidence
125439		96.7
125438		95.555

Halophily

@ref	Salt	Growth	Tested relation	Concentration
44019	NaCl	positive	growth	0-2 %
44019	NaCl	positive	optimum	0-1 %

Observation

44019

Observationrespiratory quinone is Q-8 (no other quinones are detected)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
44019	16808 ChEBI	2-dehydro-D-gluconate	-	assimilation
44019		3-O-methyl alpha-D-glucopyranoside	-	assimilation
44019	58143 ChEBI	5-dehydro-D-gluconate	-	assimilation
44019	17128 ChEBI	adipate	+	assimilation
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
44019	27613 ChEBI	amygdalin	-	assimilation
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
44019	17057 ChEBI	cellobiose	-	assimilation
44019	17108 ChEBI	D-arabinose	-	assimilation
44019	18333 ChEBI	D-arabitol	-	assimilation
44019	15824 ChEBI	D-fructose	-	assimilation
44019	28847 ChEBI	D-fucose	-	assimilation
44019	12936 ChEBI	D-galactose	-	assimilation
44019	17634 ChEBI	D-glucose	-	assimilation
44019	17634 ChEBI	D-glucose	-	builds acid from
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
44019	62318 ChEBI	D-lyxose	-	assimilation
44019	16899 ChEBI	D-mannitol	-	assimilation
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
44019	16024 ChEBI	D-mannose	-	assimilation
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
44019	16988 ChEBI	D-ribose	-	assimilation
44019	17924 ChEBI	D-sorbitol	-	assimilation
44019	16443 ChEBI	D-tagatose	-	assimilation
44019	65327 ChEBI	D-xylose	-	assimilation
44019	27689 ChEBI	decanoate	-	assimilation
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
44019	17113 ChEBI	erythritol	-	assimilation
44019	4853 ChEBI	esculin	-	assimilation
44019	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
44019	16813 ChEBI	galactitol	-	assimilation
44019	5291 ChEBI	gelatin	-	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
44019	28066 ChEBI	gentiobiose	-	assimilation
44019	24265 ChEBI	gluconate	-	assimilation
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
44019	17234 ChEBI	glucose	-	fermentation
44019	17754 ChEBI	glycerol	-	assimilation
44019	28087 ChEBI	glycogen	-	assimilation
44019	15443 ChEBI	inulin	-	assimilation
44019	30849 ChEBI	L-arabinose	-	assimilation
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
44019	18403 ChEBI	L-arabitol	-	assimilation
44019	18287 ChEBI	L-fucose	-	assimilation
44019	62345 ChEBI	L-rhamnose	-	assimilation
44019	17266 ChEBI	L-sorbose	-	assimilation
44019	65328 ChEBI	L-xylose	-	assimilation
44019	17716 ChEBI	lactose	-	assimilation
44019	25115 ChEBI	malate	-	assimilation
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
44019	17306 ChEBI	maltose	-	assimilation
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
44019	6731 ChEBI	melezitose	-	assimilation
44019	28053 ChEBI	melibiose	-	assimilation
44019	43943 ChEBI	methyl alpha-D-mannoside	-	assimilation
44019	74863 ChEBI	methyl beta-D-xylopyranoside	-	assimilation
44019	17268 ChEBI	myo-inositol	-	assimilation
44019	506227 ChEBI	N-acetylglucosamine	-	assimilation
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
44019	18401 ChEBI	phenylacetate	-	assimilation
44019	16634 ChEBI	raffinose	-	assimilation
44019	15963 ChEBI	ribitol	-	assimilation
44019	17814 ChEBI	salicin	-	assimilation
44019	53258 ChEBI	sodium citrate	-	assimilation
44019	28017 ChEBI	starch	-	assimilation
44019	27082 ChEBI	trehalose	-	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
44019	32528 ChEBI	turanose	-	assimilation
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
44019	17151 ChEBI	xylitol	-	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite
44019	35581 ChEBI	indole
68369	35581 ChEBI	indole	from API 20NE
44019	16301 ChEBI	nitrite

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
44019	acid phosphatase	+	3.1.3.2
44019	alkaline phosphatase	+	3.1.3.1
44019	alpha-chymotrypsin	+	3.4.21.1
44019	alpha-fucosidase	-	3.2.1.51
44019	alpha-galactosidase	-	3.2.1.22
44019	alpha-glucosidase	-	3.2.1.20
44019	alpha-mannosidase	-	3.2.1.24
44019	arginine dihydrolase	+	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
44019	beta-glucosidase	-	3.2.1.21
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
44019	beta-glucuronidase	-	3.2.1.31
44019	catalase	+	1.11.1.6
44019	cystine arylamidase	+	3.4.11.3
44019	cytochrome oxidase	+	1.9.3.1
44019	esterase (C 4)	+
44019	esterase Lipase (C 8)	+
68369	gelatinase	-		from API 20NE
44019	leucine arylamidase	+	3.4.11.1
44019	lipase (C 14)	-
44019	N-acetyl-beta-glucosaminidase	-	3.2.1.52
44019	naphthol-AS-BI-phosphohydrolase	+
44019	trypsin	-	3.4.21.4
44019	urease	+	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
44019	valine arylamidase	-

Fatty acid profile

Metadata FA analysis

@ref

44019

Fatty acid	Percentage
C16:1ω7c	32.3
C16:0	21.2
cycloC17:0	20.5
C18:1ω7c	14.5
C14:0	4
11-methyl-C18:1ω7c	2.9
C12:0	2.6
C16:0 iso	2.1

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
64934	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge
#Engineered	#Bioreactor	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date	Isolation procedure
44019	activated sludge bioreactor which treated landfill leachate	landfill leachate originated from Transylvania, Odorheiu Secuiesc (Harghita County)	Romania	ROU	Europe	June 2013	for isolation, a diluted R2A-based medium was used, which contained 360 ml R2A medium (DSMZ medium 830), 1.33 g CaCl2 and 1.81 g NH4Cl in 1L final volume (pH 8.0), and was solidified with 10 g/L gellangum (Gelzan CM, Sigma)
64934	landfill leachate-treating bioreactor		Romania	ROU	Europe
67770	Activated sludge sample of a bioreactor which treated the leachate of a landfill site located in Odorheiu Secuiesc	Harghita County	Romania	ROU	Europe

Taxonmaps

69479

Global distribution of 16S sequence KM083133 (>99% sequence identity) for Quisquiliibacterium transsilvanicum subclade from Microbeatlas

Aquatic Samples	132
Soil Samples	49
Animal Samples	31
Plant Samples	11
Total Samples	223

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64934	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1420221v1 assembly for Quisquiliibacterium transsilvanicum DSM 29781	scaffold	GCA_014202215	1549638.3	2861366533	1549638	72.61
124043	ASM3954035v1 assembly for Quisquiliibacterium transsilvanicum JCM 31785	scaffold	GCA_039540355			1549638	66.7

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64934	Quisquiliibacterium transsilvanicum strain CGI-09 16S ribosomal RNA gene, partial sequence	KM083133	1453		1549638

GC content

@ref	GC-content (mol%)	Method
44019	70.2
64934	70.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Quisquiliibacterium transsilvanicum DSM 29781
NCBI: GCA_014202215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.70	no
125439	motility	BacteriaNetⓘ	yes	74.00	no
125439	gram_stain	BacteriaNetⓘ	negative	92.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	92.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Quisquiliibacterium transsilvanicum DSM 29781
NCBI: GCA_014202215.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.00	no
125438	anaerobic	anaerobicⓘ	no	91.52	yes
125438	aerobic	aerobicⓘ	yes	82.83	yes
125438	spore-forming	spore-formingⓘ	no	95.56	no
125438	thermophilic	thermophileⓘ	no	94.55	yes
125438	flagellated	motile2+ⓘ	no	65.42	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Zeimonas sediminis sp. nov., isolated from mangrove sediment.	Li J, Liu L, Huang Y, Pan J, Li M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005673	2023
Phylogeny	Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor.	Felfoldi T, Fiko RD, Mentes A, Kovacs E, Mathe I, Schumann P, Toth E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002368	2017
Phylogeny	Zeimonas arvi gen. nov., sp. nov., of the family Burkholderiaceae, harboring biphenyl- and phenolic acid-metabolizing genes, isolated from a long-term ecological research field.	Lin SY, Hameed A, Tsai CF, Young CC	Antonie Van Leeuwenhoek	10.1007/s10482-021-01664-x	2021

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#44019	Tamás Felföldi, Róbert Dezso Fikó, Anikó Mentes, Erika Kovács, István Máthé, Peter Schumann, Erika Tóth: Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor. IJSEM 67: 4742 - 4746 2017 ( DOI 10.1099/ijsem.0.002368 , PubMed 28950929 )
#64934	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29781
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Quisquiliibacterium transsilvanicum (DSM 29781, JCM 31785, CGI-09)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014202215

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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