Strain identifier
BacDive ID: 141111
Type strain:
Species: Quisquiliibacterium transsilvanicum
Strain Designation: CGI-09
Strain history: T. Felföldi; Eötvös Loránd Univ., Hungary; CGI-09.
NCBI tax ID(s): 1549638 (species)
General
@ref: 64934
BacDive-ID: 141111
DSM-Number: 29781
keywords: genome sequence, 16S sequence, Bacteria, aerobe, rod-shaped, colony-forming
description: Quisquiliibacterium transsilvanicum CGI-09 is an aerobe, rod-shaped bacterium that forms circular colonies and was isolated from activated sludge bioreactor which treated landfill leachate.
NCBI tax id
- NCBI tax id: 1549638
- Matching level: species
strain history
@ref | history |
---|---|
64934 | <- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary; CGI-09 <- I. Máthé and R.-D. Fikó, |
67770 | T. Felföldi; Eötvös Loránd Univ., Hungary; CGI-09. |
doi: 10.13145/bacdive141111.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Quisquiliibacterium
- species: Quisquiliibacterium transsilvanicum
- full scientific name: Quisquiliibacterium transsilvanicum Felföldi et al. 2017
@ref: 64934
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Quisquiliibacterium
species: Quisquiliibacterium transsilvanicum
full scientific name: Quisquiliibacterium transsilvanicum Felföldi et al. 2017
strain designation: CGI-09
type strain: yes
Morphology
cell morphology
@ref | cell length | cell width | cell shape | motility | gram stain | confidence |
---|---|---|---|---|---|---|
44019 | 1.0-1..2 µm | 0.6-0.7 µm | rod-shaped | no | ||
44019 | negative | |||||
69480 | negative | 100 |
colony morphology
- @ref: 44019
- colony size: 1 mm
- colony color: beige
- colony shape: circular
- incubation period: 4-6 days
- medium used: nutrient medium
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
44019 | nutrient medium | yes | DSM medium 1, pH 7 | |
64934 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base | https://mediadive.dsmz.de/medium/693 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
44019 | positive | growth | 10-45 | |
44019 | positive | optimum | 25-30 | mesophilic |
64934 | positive | growth | 20 | psychrophilic |
67770 | positive | growth | 21 | psychrophilic |
culture pH
- @ref: 44019
- ability: positive
- type: growth
- pH: 7.0-8.0
Physiology and metabolism
oxygen tolerance
- @ref: 44019
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.997 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
44019 | NaCl | positive | growth | 0-2 % |
44019 | NaCl | positive | optimum | 0-1 % |
observation
- @ref: 44019
- observation: respiratory quinone is Q-8 (no other quinones are detected)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
44019 | 16808 | 2-dehydro-D-gluconate | - | assimilation |
44019 | 3-O-methyl alpha-D-glucopyranoside | - | assimilation | |
44019 | 58143 | 5-dehydro-D-gluconate | - | assimilation |
44019 | 27613 | amygdalin | - | assimilation |
44019 | 27689 | decanoate | - | assimilation |
44019 | 17057 | cellobiose | - | assimilation |
44019 | 15963 | ribitol | - | assimilation |
44019 | 17108 | D-arabinose | - | assimilation |
44019 | 18333 | D-arabitol | - | assimilation |
44019 | 15824 | D-fructose | - | assimilation |
44019 | 28847 | D-fucose | - | assimilation |
44019 | 12936 | D-galactose | - | assimilation |
44019 | 17634 | D-glucose | - | assimilation |
44019 | 62318 | D-lyxose | - | assimilation |
44019 | 16899 | D-mannitol | - | assimilation |
44019 | 16024 | D-mannose | - | assimilation |
44019 | 16988 | D-ribose | - | assimilation |
44019 | 17924 | D-sorbitol | - | assimilation |
44019 | 16443 | D-tagatose | - | assimilation |
44019 | 65327 | D-xylose | - | assimilation |
44019 | 16813 | galactitol | - | assimilation |
44019 | 17113 | erythritol | - | assimilation |
44019 | 4853 | esculin | - | assimilation |
44019 | 28066 | gentiobiose | - | assimilation |
44019 | 24265 | gluconate | - | assimilation |
44019 | 17754 | glycerol | - | assimilation |
44019 | 28087 | glycogen | - | assimilation |
44019 | 17268 | myo-inositol | - | assimilation |
44019 | 15443 | inulin | - | assimilation |
44019 | 30849 | L-arabinose | - | assimilation |
44019 | 18403 | L-arabitol | - | assimilation |
44019 | 18287 | L-fucose | - | assimilation |
44019 | 62345 | L-rhamnose | - | assimilation |
44019 | 17266 | L-sorbose | - | assimilation |
44019 | 65328 | L-xylose | - | assimilation |
44019 | 17716 | lactose | - | assimilation |
44019 | 25115 | malate | - | assimilation |
44019 | 17306 | maltose | - | assimilation |
44019 | 6731 | melezitose | - | assimilation |
44019 | 28053 | melibiose | - | assimilation |
44019 | 43943 | methyl alpha-D-mannoside | - | assimilation |
44019 | 74863 | methyl beta-D-xylopyranoside | - | assimilation |
44019 | 506227 | N-acetylglucosamine | - | assimilation |
44019 | 18401 | phenylacetate | - | assimilation |
44019 | 16634 | raffinose | - | assimilation |
44019 | 17814 | salicin | - | assimilation |
44019 | 53258 | sodium citrate | - | assimilation |
44019 | 28017 | starch | - | assimilation |
44019 | 32528 | turanose | - | assimilation |
44019 | 17151 | xylitol | - | assimilation |
44019 | 27082 | trehalose | - | assimilation |
44019 | 17634 | D-glucose | - | builds acid from |
44019 | 17234 | glucose | - | fermentation |
44019 | 4853 | esculin | - | hydrolysis |
44019 | 5291 | gelatin | - | hydrolysis |
44019 | 17128 | adipate | + | assimilation |
68369 | 25115 | malate | - | assimilation |
68369 | 17128 | adipate | + | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | - | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
44019 | 35581 | indole | no |
44019 | 16301 | nitrite | yes |
68369 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
44019 | acid phosphatase | + | 3.1.3.2 |
44019 | alkaline phosphatase | + | 3.1.3.1 |
44019 | arginine dihydrolase | + | 3.5.3.6 |
44019 | alpha-chymotrypsin | + | 3.4.21.1 |
44019 | cystine arylamidase | + | 3.4.11.3 |
44019 | esterase (C 4) | + | |
44019 | esterase Lipase (C 8) | + | |
44019 | leucine arylamidase | + | 3.4.11.1 |
44019 | naphthol-AS-BI-phosphohydrolase | + | |
44019 | urease | + | 3.5.1.5 |
44019 | alpha-fucosidase | - | 3.2.1.51 |
44019 | alpha-galactosidase | - | 3.2.1.22 |
44019 | beta-glucuronidase | - | 3.2.1.31 |
44019 | alpha-glucosidase | - | 3.2.1.20 |
44019 | beta-glucosidase | - | 3.2.1.21 |
44019 | lipase (C 14) | - | |
44019 | alpha-mannosidase | - | 3.2.1.24 |
44019 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
44019 | trypsin | - | 3.4.21.4 |
44019 | valine arylamidase | - | |
44019 | cytochrome oxidase | + | 1.9.3.1 |
44019 | catalase | + | 1.11.1.6 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage 44019 11-methyl-C18:1ω7c 2.9 44019 C12:0 2.6 44019 C14:0 4 44019 C16:0 21.2 44019 C16:1ω7c 32.3 44019 C18:1ω7c 14.5 44019 cycloC17:0 20.5 44019 C16:0 iso 2.1 - type of FA analysis: whole cell analysis
- incubation medium: TSA
- agar/liquid: agar
- incubation temperature: 25
- incubation time: 6
- system: MIS MIDI
- cutoff value:
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
64934 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date | isolation procedure |
---|---|---|---|---|---|---|---|
44019 | activated sludge bioreactor which treated landfill leachate | landfill leachate originated from Transylvania, Odorheiu Secuiesc (Harghita County) | Romania | ROU | Europe | June 2013 | for isolation, a diluted R2A-based medium was used, which contained 360 ml R2A medium (DSMZ medium 830), 1.33 g CaCl2 and 1.81 g NH4Cl in 1L final volume (pH 8.0), and was solidified with 10 g/L gellangum (Gelzan CM, Sigma) |
64934 | landfill leachate-treating bioreactor | Romania | ROU | Europe | |||
67770 | Activated sludge sample of a bioreactor which treated the leachate of a landfill site located in Odorheiu Secuiesc | Harghita County | Romania | ROU | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Waste | #Activated sludge |
#Engineered | #Bioreactor |
taxonmaps
- @ref: 69479
- File name: preview.99_51288.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_8560;97_10297;98_12637;99_51288&stattab=map
- Last taxonomy: Quisquiliibacterium transsilvanicum subclade
- 16S sequence: KM083133
- Sequence Identity:
- Total samples: 223
- soil counts: 49
- aquatic counts: 132
- animal counts: 31
- plant counts: 11
Safety information
risk assessment
- @ref: 64934
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 64934
- description: Quisquiliibacterium transsilvanicum strain CGI-09 16S ribosomal RNA gene, partial sequence
- accession: KM083133
- length: 1453
- database: ena
- NCBI tax ID: 1549638
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Quisquiliibacterium transsilvanicum DSM 29781 | GCA_014202215 | scaffold | ncbi | 1549638 |
66792 | Quisquiliibacterium transsilvanicum strain DSM 29781 | 1549638.3 | wgs | patric | 1549638 |
66792 | Quisquiliibacterium transsilvanicum DSM 29781 | 2861366533 | draft | img | 1549638 |
GC content
@ref | GC-content | method |
---|---|---|
44019 | 70.2 | |
64934 | 70.2 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 91.128 | yes |
gram-positive | no | 98.509 | no |
anaerobic | no | 95.937 | yes |
aerobic | yes | 82.895 | yes |
halophile | no | 88.391 | no |
spore-forming | no | 96.779 | no |
glucose-util | no | 87.359 | yes |
thermophile | no | 99.407 | no |
glucose-ferment | no | 90.846 | yes |
motile | no | 82.318 | no |
External links
@ref: 64934
culture collection no.: DSM 29781, JCM 31785
straininfo link
- @ref: 96859
- straininfo: 406943
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 28950929 | Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor. | Felfoldi T, Fiko RD, Mentes A, Kovacs E, Mathe I, Schumann P, Toth E | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002368 | 2017 | Alcaligenaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Romania, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry | Transcriptome |
Phylogeny | 34599477 | Zeimonas arvi gen. nov., sp. nov., of the family Burkholderiaceae, harboring biphenyl- and phenolic acid-metabolizing genes, isolated from a long-term ecological research field. | Lin SY, Hameed A, Tsai CF, Young CC | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01664-x | 2021 | Bacterial Typing Techniques, Biphenyl Compounds, *Burkholderiaceae, DNA, Bacterial/genetics, Fatty Acids, Hydroxybenzoates, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
44019 | Tamás Felföldi, Róbert Dezso Fikó, Anikó Mentes, Erika Kovács, István Máthé, Peter Schumann, Erika Tóth | Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor | 10.1099/ijsem.0.002368 | IJSEM 67: 4742-4746 2017 | 28950929 | |
64934 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29781 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29781) | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68369 | Automatically annotated from API 20NE | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
96859 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406943.1 |