Strain identifier

BacDive ID: 141111

Type strain: Yes

Species: Quisquiliibacterium transsilvanicum

Strain Designation: CGI-09

Strain history: T. Felföldi; Eötvös Loránd Univ., Hungary; CGI-09.

NCBI tax ID(s): 1549638 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 64934

BacDive-ID: 141111

DSM-Number: 29781

keywords: genome sequence, 16S sequence, Bacteria, aerobe, rod-shaped, colony-forming

description: Quisquiliibacterium transsilvanicum CGI-09 is an aerobe, rod-shaped bacterium that forms circular colonies and was isolated from activated sludge bioreactor which treated landfill leachate.

NCBI tax id

  • NCBI tax id: 1549638
  • Matching level: species

strain history

@refhistory
64934<- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary; CGI-09 <- I. Máthé and R.-D. Fikó,
67770T. Felföldi; Eötvös Loránd Univ., Hungary; CGI-09.

doi: 10.13145/bacdive141111.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Quisquiliibacterium
  • species: Quisquiliibacterium transsilvanicum
  • full scientific name: Quisquiliibacterium transsilvanicum Felföldi et al. 2017

@ref: 64934

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Quisquiliibacterium

species: Quisquiliibacterium transsilvanicum

full scientific name: Quisquiliibacterium transsilvanicum Felföldi et al. 2017

strain designation: CGI-09

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilitygram stainconfidence
440191.0-1..2 µm0.6-0.7 µmrod-shapedno
44019negative
69480negative100

colony morphology

  • @ref: 44019
  • colony size: 1 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 4-6 days
  • medium used: nutrient medium

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
44019nutrient mediumyesDSM medium 1, pH 7
64934COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693

culture temp

@refgrowthtypetemperaturerange
44019positivegrowth10-45
44019positiveoptimum25-30mesophilic
64934positivegrowth20psychrophilic
67770positivegrowth21psychrophilic

culture pH

  • @ref: 44019
  • ability: positive
  • type: growth
  • pH: 7.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 44019
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.997

halophily

@refsaltgrowthtested relationconcentration
44019NaClpositivegrowth0-2 %
44019NaClpositiveoptimum0-1 %

observation

  • @ref: 44019
  • observation: respiratory quinone is Q-8 (no other quinones are detected)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
44019168082-dehydro-D-gluconate-assimilation
440193-O-methyl alpha-D-glucopyranoside-assimilation
44019581435-dehydro-D-gluconate-assimilation
4401927613amygdalin-assimilation
4401927689decanoate-assimilation
4401917057cellobiose-assimilation
4401915963ribitol-assimilation
4401917108D-arabinose-assimilation
4401918333D-arabitol-assimilation
4401915824D-fructose-assimilation
4401928847D-fucose-assimilation
4401912936D-galactose-assimilation
4401917634D-glucose-assimilation
4401962318D-lyxose-assimilation
4401916899D-mannitol-assimilation
4401916024D-mannose-assimilation
4401916988D-ribose-assimilation
4401917924D-sorbitol-assimilation
4401916443D-tagatose-assimilation
4401965327D-xylose-assimilation
4401916813galactitol-assimilation
4401917113erythritol-assimilation
440194853esculin-assimilation
4401928066gentiobiose-assimilation
4401924265gluconate-assimilation
4401917754glycerol-assimilation
4401928087glycogen-assimilation
4401917268myo-inositol-assimilation
4401915443inulin-assimilation
4401930849L-arabinose-assimilation
4401918403L-arabitol-assimilation
4401918287L-fucose-assimilation
4401962345L-rhamnose-assimilation
4401917266L-sorbose-assimilation
4401965328L-xylose-assimilation
4401917716lactose-assimilation
4401925115malate-assimilation
4401917306maltose-assimilation
440196731melezitose-assimilation
4401928053melibiose-assimilation
4401943943methyl alpha-D-mannoside-assimilation
4401974863methyl beta-D-xylopyranoside-assimilation
44019506227N-acetylglucosamine-assimilation
4401918401phenylacetate-assimilation
4401916634raffinose-assimilation
4401917814salicin-assimilation
4401953258sodium citrate-assimilation
4401928017starch-assimilation
4401932528turanose-assimilation
4401917151xylitol-assimilation
4401927082trehalose-assimilation
4401917634D-glucose-builds acid from
4401917234glucose-fermentation
440194853esculin-hydrolysis
440195291gelatin-hydrolysis
4401917128adipate+assimilation
6836925115malate-assimilation
6836917128adipate+assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
4401935581indoleno
4401916301nitriteyes
6836935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
44019acid phosphatase+3.1.3.2
44019alkaline phosphatase+3.1.3.1
44019arginine dihydrolase+3.5.3.6
44019alpha-chymotrypsin+3.4.21.1
44019cystine arylamidase+3.4.11.3
44019esterase (C 4)+
44019esterase Lipase (C 8)+
44019leucine arylamidase+3.4.11.1
44019naphthol-AS-BI-phosphohydrolase+
44019urease+3.5.1.5
44019alpha-fucosidase-3.2.1.51
44019alpha-galactosidase-3.2.1.22
44019beta-glucuronidase-3.2.1.31
44019alpha-glucosidase-3.2.1.20
44019beta-glucosidase-3.2.1.21
44019lipase (C 14)-
44019alpha-mannosidase-3.2.1.24
44019N-acetyl-beta-glucosaminidase-3.2.1.52
44019trypsin-3.4.21.4
44019valine arylamidase-
44019cytochrome oxidase+1.9.3.1
44019catalase+1.11.1.6
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    4401911-methyl-C18:1ω7c2.9
    44019C12:02.6
    44019C14:04
    44019C16:021.2
    44019C16:1ω7c32.3
    44019C18:1ω7c14.5
    44019cycloC17:020.5
    44019C16:0 iso2.1
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 6
  • system: MIS MIDI
  • cutoff value:

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
64934----------------+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation dateisolation procedure
44019activated sludge bioreactor which treated landfill leachatelandfill leachate originated from Transylvania, Odorheiu Secuiesc (Harghita County)RomaniaROUEuropeJune 2013for isolation, a diluted R2A-based medium was used, which contained 360 ml R2A medium (DSMZ medium 830), 1.33 g CaCl2 and 1.81 g NH4Cl in 1L final volume (pH 8.0), and was solidified with 10 g/L gellangum (Gelzan CM, Sigma)
64934landfill leachate-treating bioreactorRomaniaROUEurope
67770Activated sludge sample of a bioreactor which treated the leachate of a landfill site located in Odorheiu SecuiescHarghita CountyRomaniaROUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Engineered#Bioreactor

taxonmaps

  • @ref: 69479
  • File name: preview.99_51288.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_8560;97_10297;98_12637;99_51288&stattab=map
  • Last taxonomy: Quisquiliibacterium transsilvanicum subclade
  • 16S sequence: KM083133
  • Sequence Identity:
  • Total samples: 223
  • soil counts: 49
  • aquatic counts: 132
  • animal counts: 31
  • plant counts: 11

Safety information

risk assessment

  • @ref: 64934
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64934
  • description: Quisquiliibacterium transsilvanicum strain CGI-09 16S ribosomal RNA gene, partial sequence
  • accession: KM083133
  • length: 1453
  • database: ena
  • NCBI tax ID: 1549638

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Quisquiliibacterium transsilvanicum DSM 29781GCA_014202215scaffoldncbi1549638
66792Quisquiliibacterium transsilvanicum strain DSM 297811549638.3wgspatric1549638
66792Quisquiliibacterium transsilvanicum DSM 297812861366533draftimg1549638

GC content

@refGC-contentmethod
4401970.2
6493470.2high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno91.128yes
gram-positiveno98.509no
anaerobicno95.937yes
aerobicyes82.895yes
halophileno88.391no
spore-formingno96.779no
glucose-utilno87.359yes
thermophileno99.407no
glucose-fermentno90.846yes
motileno82.318no

External links

@ref: 64934

culture collection no.: DSM 29781, JCM 31785

straininfo link

  • @ref: 96859
  • straininfo: 406943

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28950929Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor.Felfoldi T, Fiko RD, Mentes A, Kovacs E, Mathe I, Schumann P, Toth EInt J Syst Evol Microbiol10.1099/ijsem.0.0023682017Alcaligenaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Romania, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistryTranscriptome
Phylogeny34599477Zeimonas arvi gen. nov., sp. nov., of the family Burkholderiaceae, harboring biphenyl- and phenolic acid-metabolizing genes, isolated from a long-term ecological research field.Lin SY, Hameed A, Tsai CF, Young CCAntonie Van Leeuwenhoek10.1007/s10482-021-01664-x2021Bacterial Typing Techniques, Biphenyl Compounds, *Burkholderiaceae, DNA, Bacterial/genetics, Fatty Acids, Hydroxybenzoates, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44019Tamás Felföldi, Róbert Dezso Fikó, Anikó Mentes, Erika Kovács, István Máthé, Peter Schumann, Erika TóthQuisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor10.1099/ijsem.0.002368IJSEM 67: 4742-4746 201728950929
64934Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29781Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29781)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96859Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406943.1