Nitrosopumilus maritimus (ATCC TSD-97, NCIMB 15022, SCM 1)

Name: Nitrosopumilus maritimus (ATCC TSD-97, NCIMB 15022, SCM 1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141102

Type strain

Culture col. no. ATCC TSD-97 NCIMB 15022 SCM 1

NCBI tax ID(s) 338192

Special Collections Literature strains

Nitrosopumilus maritimus ATCC TSD-97 is an aerobe, chemoautolithotroph, rod-shaped archaeon that was isolated from a tropical marine fish tank at the Seattle Aquarium in Seattle.

rod-shaped aerobe chemoautolithotroph genome sequence Archaea

Name and taxonomic classification

Culture Collection Numbers

ATCC TSD-97 NCIMB 15022 SCM 1

@ref 20215

Last LPSN update 2026-02-09 11:33:49

Domain Archaea

Phylum Thermoproteota

Class Nitrososphaeria

Order Nitrosopumilales

Family Nitrosopumilaceae

Genus Nitrosopumilus

Species Nitrosopumilus maritimus

Full scientific name Nitrosopumilus maritimus Qin et al. 2017

Synonyms (1)

"Candidatus Nitrosopumilus maritimus"

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Motility
44010	0.5-0.9 µm	0.17-0.22 µm	rod-shaped

Colony morphology

Multicellular morphology

44010

Forms multicellular complexno

Culture and growth conditions

Culture medium

@ref	Growth	Composition
44010		26 g/L NaCl, 5 g/L MgSO4 6H2O, 1.5 g/L CaCl 2x2H2O, 0.1 g/L KBr, 10 ml/L HEPES buffer (1 M HEPES and 0.6 M NaOH), 2 ml/L NaHCO3 (1 M), 5 ml/L KH2PO4 (0.4 g/L), 1 ml/L FeNaEDTA solution (7.5 M), 1 ml/L trace element solution, and 1 ml/L NH 4 Cl (1 M)

Culture temp

@ref	Growth	Type	Temperature (°C)
44010	positive	growth	15-35
44010	positive	optimum	32
44010

Culture pH

@ref	Ability	Type	PH
44010	positive	optimum	7.3
44010	positive	growth	6.8-8.1

Physiology and metabolism

Oxygen tolerance

44010

Oxygen toleranceaerobe

Nutrition type

44010

Typechemoautolithotroph

Halophily

@ref	Salt	Growth	Tested relation	Concentration	Halophily level
44010	NaCl	positive	optimum	32-37 %
44010					moderately halophilic

Observation

@ref	Observation
44010	the cell envelope consists of an S-layer with p6 symmetry covering a monolayer cytoplasmic membrane
44010	the minimum generation time is around 19 h
44010	ectoine and hydroxyectoine are synthesized in cells in response to osmotic shock
44010	cells are photosensitive and completely inhibited by continuous illumination
44010	respiratory quinones are saturated and monounsaturated menaquinones with 6 isoprenoid units
44010	free-living in a wide range of marine systems, including surface oceans, hadal oceans, saltwater aquaria, brackish waters, marine and estuarine sediments, salt marshes, and brine-seawater interfaces

Tolerance

@ref	Compound
44010	ammonia
44010	nitrite

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
44010	16134 ChEBI	ammonia	+	oxidation
44010	16526 ChEBI	carbon dioxide	+	carbon source
44010	16199 ChEBI	urea	+	nitrogen source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
44010	30411 ChEBI	cobalamin

Enzymes

@ref	Value	Activity	Ec
44010	urease	-	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Climate	#Hot	#Tropical
#Engineered	#Built environment	#Water reservoir (Aquarium/pool)
#Environmental	#Aquatic	#Marine

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
44010	a tropical marine fish tank at the Seattle Aquarium in Seattle	Washington	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence CP000866 (>99% sequence identity) for Nitrosopumilus from Microbeatlas

Aquatic Samples	3053
Soil Samples	75
Animal Samples	2
Plant Samples	3
Total Samples	3133

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
44010	ASM1846v1 assembly for Nitrosopumilus maritimus SCM1	complete	GCA_000018465	436308.17	641228499	436308	98.98

GC content

44010

GC-content (mol%)34.2

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Geographic Distribution of Ammonia-Oxidizing Archaea along the Kuril Islands in the Western Subarctic Pacific.	Jing H, Cheung S, Xia X, Suzuki K, Nishioka J, Liu H.	Front Microbiol	10.3389/fmicb.2017.01247	2017
Metabolism	Functional analysis of archaeal MBF1 by complementation studies in yeast.	Marrero Coto J, Ehrenhofer-Murray AE, Pons T, Siebers B	Biol Direct	10.1186/1745-6150-6-18	2011
	An evidence of laccases in archaea.	Sharma KK, Kuhad RC	Indian J Microbiol	10.1007/s12088-009-0039-4	2009
	Distribution and Oxidation Rates of Ammonia-Oxidizing Archaea Influenced by the Coastal Upwelling off Eastern Hainan Island.	Liu H, Zhou P, Cheung S, Lu Y, Liu H, Jing H	Microorganisms	10.3390/microorganisms10050952	2022
	A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry.	Law KP, He W, Tao J, Zhang C	Front Microbiol	10.3389/fmicb.2021.735878	2021
Metabolism	Structural insights into bifunctional thaumarchaeal crotonyl-CoA hydratase and 3-hydroxypropionyl-CoA dehydratase from Nitrosopumilus maritimus.	Destan E, Yuksel B, Tolar BB, Ayan E, Deutsch S, Yoshikuni Y, Wakatsuki S, Francis CA, DeMirci H	Sci Rep	10.1038/s41598-021-02180-8	2021
	Characterization of the Exometabolome of Nitrosopumilus maritimus SCM1 by Liquid Chromatography-Ion Mobility Mass Spectrometry.	Law KP, He W, Tao J, Zhang C	Front Microbiol	10.3389/fmicb.2021.658781	2021
Metabolism	Energy flux controls tetraether lipid cyclization in Sulfolobus acidocaldarius.	Zhou A, Weber Y, Chiu BK, Elling FJ, Cobban AB, Pearson A, Leavitt WD	Environ Microbiol	10.1111/1462-2920.14851	2019
Metabolism	Iron requirements and uptake strategies of the globally abundant marine ammonia-oxidising archaeon, Nitrosopumilus maritimus SCM1.	Shafiee RT, Snow JT, Zhang Q, Rickaby REM	ISME J	10.1038/s41396-019-0434-8	2019
Genetics	Genomic adaptation to eutrophication of ammonia-oxidizing archaea in the Pearl River estuary.	Zou D, Li Y, Kao SJ, Liu H, Li M	Environ Microbiol	10.1111/1462-2920.14613	2019
Biotechnology	Genome-scale metabolic model analysis indicates low energy production efficiency in marine ammonia-oxidizing archaea.	Li F, Xie W, Yuan Q, Luo H, Li P, Chen T, Zhao X, Wang Z, Ma H	AMB Express	10.1186/s13568-018-0635-y	2018
Metabolism	Primer selection influences abundance estimates of ammonia oxidizing archaea in coastal marine sediments.	Marshall A, Phillips L, Longmore A, Tang C, Heidelberg K, Mele P	Mar Environ Res	10.1016/j.marenvres.2018.06.001	2018
Metabolism	Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations.	Qin W, Amin SA, Lundeen RA, Heal KR, Martens-Habbena W, Turkarslan S, Urakawa H, Costa KC, Hendrickson EL, Wang T, Beck DA, Tiquia-Arashiro SM, Taub F, Holmes AD, Vajrala N, Berube PM, Lowe TM, Moffett JW, Devol AH, Baliga NS, Arp DJ, Sayavedra-Soto LA, Hackett M, Armbrust EV, Ingalls AE, Stahl DA	ISME J	10.1038/ismej.2017.186	2017
Metabolism	Kinetic analysis of a complete nitrifier reveals an oligotrophic lifestyle.	Kits KD, Sedlacek CJ, Lebedeva EV, Han P, Bulaev A, Pjevac P, Daebeler A, Romano S, Albertsen M, Stein LY, Daims H, Wagner M	Nature	10.1038/nature23679	2017
Metabolism	Influence of ammonia oxidation rate on thaumarchaeal lipid composition and the TEX86 temperature proxy.	Hurley SJ, Elling FJ, Konneke M, Buchwald C, Wankel SD, Santoro AE, Lipp JS, Hinrichs KU, Pearson A	Proc Natl Acad Sci U S A	10.1073/pnas.1518534113	2016
	A Purple Cupredoxin from Nitrosopumilus maritimus Containing a Mononuclear Type 1 Copper Center with an Open Binding Site.	Hosseinzadeh P, Tian S, Marshall NM, Hemp J, Mullen T, Nilges MJ, Gao YG, Robinson H, Stahl DA, Gennis RB, Lu Y	J Am Chem Soc	10.1021/jacs.5b13128	2016
Phylogeny	The influence of salinity on the abundance, transcriptional activity, and diversity of AOA and AOB in an estuarine sediment: a microcosm study.	Zhang Y, Chen L, Dai T, Tian J, Wen D	Appl Microbiol Biotechnol	10.1007/s00253-015-6804-x	2015
Enzymology	[Diversity of thaumarchaeal nitrite reductase (nirK) -like genes in environments].	Huang X, Luo J, Zhao D, Lin W	Wei Sheng Wu Xue Bao		2015
Phylogeny	Changes in northern Gulf of Mexico sediment bacterial and archaeal communities exposed to hypoxia.	Devereux R, Mosher JJ, Vishnivetskaya TA, Brown SD, Beddick DL Jr, Yates DF, Palumbo AV	Geobiology	10.1111/gbi.12142	2015
Metabolism	The production of nitric oxide by marine ammonia-oxidizing archaea and inhibition of archaeal ammonia oxidation by a nitric oxide scavenger.	Martens-Habbena W, Qin W, Horak RE, Urakawa H, Schauer AJ, Moffett JW, Armbrust EV, Ingalls AE, Devol AH, Stahl DA	Environ Microbiol	10.1111/1462-2920.12677	2015
Phylogeny	Marine ammonia-oxidizing archaeal isolates display obligate mixotrophy and wide ecotypic variation.	Qin W, Amin SA, Martens-Habbena W, Walker CB, Urakawa H, Devol AH, Ingalls AE, Moffett JW, Armbrust EV, Stahl DA	Proc Natl Acad Sci U S A	10.1073/pnas.1324115111	2014
Metabolism	Thermodynamic characterization of proton-ionizable functional groups on the cell surfaces of ammonia-oxidizing bacteria and archaea.	Gorman-Lewis D, Martens-Habbena W, Stahl DA	Geobiology	10.1111/gbi.12075	2014
Metabolism	Transcriptional response of the archaeal ammonia oxidizer Nitrosopumilus maritimus to low and environmentally relevant ammonia concentrations.	Nakagawa T, Stahl DA	Appl Environ Microbiol	10.1128/AEM.02028-13	2013
Enzymology	Ammonia oxidation kinetics and temperature sensitivity of a natural marine community dominated by Archaea.	Horak RE, Qin W, Schauer AJ, Armbrust EV, Ingalls AE, Moffett JW, Stahl DA, Devol AH	ISME J	10.1038/ismej.2013.75	2013
Phylogeny	Life in the dark: metagenomic evidence that a microbial slime community is driven by inorganic nitrogen metabolism.	Tetu SG, Breakwell K, Elbourne LD, Holmes AJ, Gillings MR, Paulsen IT	ISME J	10.1038/ismej.2013.14	2013
Metabolism	Mimicking the oxygen minimum zones: stimulating interaction of aerobic archaeal and anaerobic bacterial ammonia oxidizers in a laboratory-scale model system.	Yan J, Haaijer SC, Op den Camp HJ, van Niftrik L, Stahl DA, Konneke M, Rush D, Sinninghe Damste JS, Hu YY, Jetten MS	Environ Microbiol	10.1111/j.1462-2920.2012.02894.x	2012
Metabolism	Metagenomic analysis of a complex marine planktonic thaumarchaeal community from the Gulf of Maine.	Tully BJ, Nelson WC, Heidelberg JF	Environ Microbiol	10.1111/j.1462-2920.2011.02628.x	2011
Metabolism	Ammonia-oxidizing bacteria dominates over ammonia-oxidizing archaea in a saline nitrification reactor under low DO and high nitrogen loading.	Ye L, Zhang T	Biotechnol Bioeng	10.1002/bit.23211	2011
Metabolism	Nitrogen metabolism and kinetics of ammonia-oxidizing archaea.	Martens-Habbena W, Stahl DA	Methods Enzymol	10.1016/B978-0-12-386489-5.00019-1	2011
Metabolism	Genomic signatures of fifth autotrophic carbon assimilation pathway in bathypelagic Crenarchaeota.	La Cono V, Smedile F, Ferrer M, Golyshin PN, Giuliano L, Yakimov MM	Microb Biotechnol	10.1111/j.1751-7915.2010.00186.x	2010
Metabolism	Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea.	Walker CB, de la Torre JR, Klotz MG, Urakawa H, Pinel N, Arp DJ, Brochier-Armanet C, Chain PS, Chan PP, Gollabgir A, Hemp J, Hugler M, Karr EA, Konneke M, Shin M, Lawton TJ, Lowe T, Martens-Habbena W, Sayavedra-Soto LA, Lang D, Sievert SM, Rosenzweig AC, Manning G, Stahl DA	Proc Natl Acad Sci U S A	10.1073/pnas.0913533107	2010
Phylogeny	Low genome content diversity of marine planktonic Thaumarchaeota.	Luo H, Sun Y, Hollibaugh JT, Moran MA	Environ Microbiol Rep	10.1111/1758-2229.12417	2016
Metabolism	Enrichment of a novel marine ammonia-oxidizing archaeon obtained from sand of an eelgrass zone.	Matsutani N, Nakagawa T, Nakamura K, Takahashi R, Yoshihara K, Tokuyama T	Microbes Environ	10.1264/jsme2.me10156	2011
Phylogeny	Metatranscriptomic analysis of ammonia-oxidizing organisms in an estuarine bacterioplankton assemblage.	Hollibaugh JT, Gifford S, Sharma S, Bano N, Moran MA	ISME J	10.1038/ismej.2010.172	2010
Metabolism	Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria.	Martens-Habbena W, Berube PM, Urakawa H, de la Torre JR, Stahl DA	Nature	10.1038/nature08465	2009
Phylogeny	Nitrosarchaeum koreense gen. nov., sp. nov., an aerobic and mesophilic, ammonia-oxidizing archaeon member of the phylum Thaumarchaeota isolated from agricultural soil.	Jung MY, Islam MA, Gwak JH, Kim JG, Rhee SK	Int J Syst Evol Microbiol	10.1099/ijsem.0.002926	2018
Phylogeny	Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine ammonia-oxidizing archaea of the phylum Thaumarchaeota.	Qin W, Heal KR, Ramdasi R, Kobelt JN, Martens-Habbena W, Bertagnolli AD, Amin SA, Walker CB, Urakawa H, Konneke M, Devol AH, Moffett JW, Armbrust EV, Jensen GJ, Ingalls AE, Stahl DA	Int J Syst Evol Microbiol	10.1099/ijsem.0.002416	2017
Phylogeny	Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic, ammonia-oxidizing archaeon from soil and a member of the archaeal phylum Thaumarchaeota.	Stieglmeier M, Klingl A, Alves RJE, Rittmann SKR, Melcher M, Leisch N, Schleper C	Int J Syst Evol Microbiol	10.1099/ijs.0.063172-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#44010	Wei Qin, Katherine R. Heal, Rasika Ramdasi, Julia N. Kobelt, Willm Martens-Habbena, Anthony D. Bertagnolli, Shady A. Amin, Christopher B. Walker, Hidetoshi Urakawa, Martin Könneke, Allan H. Devol, James W. Moffett, E. Virginia Armbrust, Grant J. Jensen, Anitra E. Ingalls, David A. Stahl: Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine ammonia-oxidizing archaea of the phylum Thaumarchaeota. IJSEM 67: 5067 - 5079 2017 ( DOI 10.1099/ijsem.0.002416 , PubMed 29034851 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

Rate our new design

Content

About us

Nitrosopumilus maritimus (ATCC TSD-97, NCIMB 15022, SCM 1)

Name and taxonomic classification

Culture Collection Numbers

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Halophily

Observation

Tolerance

Metabolite utilization

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_000018465

GC content

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media