Name: Nitrincola schmidtii R4-8
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 141101

Type strain:

Species: Nitrincola schmidtii

Strain Designation: R4-8

Culture col. no.: DSM 100788, NCAIM B.02626

Strain history: <- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary; R4-8 <- K. Korponai and T. Felföldi

NCBI tax ID(s): 1730894 (species)

SI-ID 398460

DSM 100788

Special Collections

Section

Nitrincola schmidtii R4-8 is a facultative anaerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from a thin layer of a purple bacterial bloom occurring in a soda pan.

colony-forming
Gram-negative
motile
rod-shaped
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Oceanospirillaceae*
	Genus	*Nitrincola*
	Species	**Nitrincola schmidtii**
	Full Scientific Name (LPSN)	Nitrincola schmidtii Borsodi et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #44009]	Gram stain	negative

[Ref.: #44009]	Cell length	1.4-1.8 µm

[Ref.: #44009]	Cell width	0.5-0.6 µm

[Ref.: #44009]	Cell shape	rod-shaped

[Ref.: #44009]	Motility	yes

[Ref.: #44009]	Colony color	beige
[Ref.: #44009]	Colony shape	circular
[Ref.: #44009]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64980]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #64980]

Culture medium growth

[Ref.: #64980]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64980]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #44009]	growth	10-28 °C
[Ref.: #44009]	optimum	15-20 °C
[Ref.: #64980]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #44009]			optimum	8.0-9.0
[Ref.: #44009]			growth	7.0-11.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44009]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44009]		NaCl		growth	0-10	%
[Ref.: #44009]		NaCl		optimum	0-7	%

[Ref.: #44009]	Observation	smooth and convex colonies
[Ref.: #44009]	Observation	the predominant quinone is Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44009]	casein	-	hydrolysis
[Ref.: #44009]	citrate ChEBI	-	assimilation
[Ref.: #44009]	D-glucose ChEBI	-	builds acid from
[Ref.: #44009]	D-glucose ChEBI	-	builds gas from
[Ref.: #44009]	esculin ferric citrate	-	hydrolysis
[Ref.: #44009]	gelatin ChEBI	-	hydrolysis
[Ref.: #44009]	nitrate ChEBI	+	reduction
[Ref.: #44009]	phenylalanine ChEBI	+	assimilation
[Ref.: #44009]	starch ChEBI	-	hydrolysis
[Ref.: #44009]	tween 80 ChEBI	-	hydrolysis
[Ref.: #44009]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #44009]		indole ChEBI	no
[Ref.: #44009]		sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #44009]	acid phosphatase	+	3.1.3.2
[Ref.: #44009]	alkaline phosphatase	+	3.1.3.1
[Ref.: #44009]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #44009]	alpha-fucosidase	+/-	3.2.1.51
[Ref.: #44009]	alpha-galactosidase	-	3.2.1.22
[Ref.: #44009]	alpha-glucosidase	-	3.2.1.20
[Ref.: #44009]	alpha-mannosidase	-	3.2.1.24
[Ref.: #44009]	beta-galactosidase	+/-	3.2.1.23
[Ref.: #44009]	beta-glucosidase	-	3.2.1.21
[Ref.: #44009]	beta-glucuronidase	-	3.2.1.31
[Ref.: #44009]	catalase	+	1.11.1.6
[Ref.: #44009]	cystine arylamidase	-	3.4.11.3
[Ref.: #44009]	cytochrome oxidase	+	1.9.3.1
[Ref.: #44009]	esterase (C 4)	+
[Ref.: #44009]	esterase Lipase (C 8)	+
[Ref.: #44009]	leucine arylamidase	+	3.4.11.1
[Ref.: #44009]	lipase (C 14)	-
[Ref.: #44009]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #44009]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #44009]	phosphatase	+
[Ref.: #44009]	trypsin	+/-	3.4.21.4
[Ref.: #44009]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44009]

Fatty acid	Percentage
C18:1ω7c	50.9
C16:1ω7c	22.7
C16:0	13.4
C10:0 3OH	5.4
C12:1	3.3
C10:0	3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44009]	Sample type/isolated from	a thin layer of a purple bacterial bloom occurring in a soda pan
[Ref.: #44009]	Geographic location (country and/or sea, region)	Kiskunsag National Park
[Ref.: #44009]	Country	Hungary
[Ref.: #44009]	Country ISO 3 Code	HUN
[Ref.: #44009]	Continent	Europe

[Ref.: #64980]	Sample type/isolated from	purple bacterial bloom occurring in a soda pan
[Ref.: #64980]	Geographic location (country and/or sea, region)	Kiskunság National Park near Soltszentimre (46° 45.818' N 19° 10.828' E)
[Ref.: #64980]	Country	Hungary
[Ref.: #64980]	Country ISO 3 Code	HUN
[Ref.: #64980]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KR233209 (>99% sequence identity) for Nitrincola schmidtii subclade from Microbeatlas

Aquatic Samples	119
Soil Samples	13
Animal Samples	57
Plant Samples
Total Samples	189

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64980]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64980]	Nitrincola schmidtii strain R4-8 16S ribosomal RNA gene, partial sequence	KR233209	1459	GenBank ENA	1730894 tax ID

[Ref.: #44009]	GC-content	45.8 mol%	sequence analysis
[Ref.: #64980]	GC-content	45.8 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64980]

Culture collection no.

DSM 100788, NCAIM B.02626

[Ref.: #96855]

SI-ID 398460

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nitrincola iocasae sp. nov., a bacterium isolated from sediment collected at a cold seep field in the South China Sea.	Zhang H, Wang H, Chen H, Sun Q, Zhong Z, Wang M, Cao L, Lian C, Zhou L, Li C	Int J Syst Evol Microbiol	10.1099/ijsem.0.004352	2020	*
Phylogeny	Nitrincola alkalilacustris sp. nov. and Nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus Nitrincola.	Borsodi AK, Korponai K, Schumann P, Sproer C, Felfoldi T, Marialigeti K, Szili-Kovacs T, Toth E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002437	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44009

Andrea K. Borsodi, Kristóf Korponai, Peter Schumann, Cathrin Spröer, Tamás Felföldi, Károly Márialigeti, Tibor Szili-Kovács, Erika Tóth: Nitrincola alkalilacustris sp. nov. and Nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus Nitrincola. IJSEM 67: 5159 - 5164 2017 ( DOI 10.1099/ijsem.0.002437 , PubMed 29056114 )

#64980

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100788

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96855

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398460.1 )

* These data were automatically processed and therefore are not curated

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