Nitrincola alkalilacustris (DSM 29817, NCAIM B.02612, NCAIM B 02612)

Name: Nitrincola alkalilacustris (DSM 29817, NCAIM B.02612, NCAIM B 02612)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141100

Type strain

Strain Designation ZV-19

Culture col. no. DSM 29817 NCAIM B.02612 NCAIM B 02612 ZV-19

NCBI tax ID(s) 1571224

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Nitrincola alkalilacustris ZV-19 is a facultative anaerobe, Gram-negative, motile prokaryote that forms circular colonies and was isolated from the alkaline water of Zab-szek soda pan.

Gram-negative motile rod-shaped colony-forming facultative anaerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 401396

NCAIM B 02612,DSM 29817

@ref 20215

Last LPSN update 2025-12-16 10:03:46

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Oceanospirillales

Family Oceanospirillaceae

Genus Nitrincola

Species Nitrincola alkalilacustris

Full scientific name Nitrincola alkalilacustris Borsodi et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
44009	negative	1.5-1.8 µm	0.5-0.8 µm	rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Incubation period	Medium used
44009	whitish-cream	circular	1-2 days	Reasoners 2A agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition
44009	Reasoners 2A agar		0.5 g yeast extract, 0.5 g proteose peptone (Difco no.3), 0.5 g casamino acids, 0.5 g glucose, 0.5 g soluble starch, 0.3 g sodium pyruvate, 0.3 g K2HPO4, 0.05g MgSO4 7H2O, 15.0 g agar and 1000.0 ml distilled water supplemented with 5 % (w/v) NaCl, and S medium containing 1.0 g yeast extract, 0.6 g MgSO4 7H2O, 0.3 g CaCl2 2H2O, 10.0 g NaHCO3, 16.0 g gellan gum (Sigma Gelzan CM) and 1000.0 ml distilled water, the pH of the media was adjusted to pH 9.0
64979	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
44009	positive	growth	10-37
44009	positive	optimum	20-28
64979	positive	growth	25

Culture pH

@ref	Ability	Type	PH
44009	positive	optimum	8.0-9.0
44009	positive	growth	7.0-11.0

Physiology and metabolism

Oxygen tolerance

44009

Oxygen tolerancefacultative anaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
44009	NaCl	positive	growth	0-10 %
44009	NaCl	positive	optimum	0-7 %

Observation

@ref	Observation
44009	smooth and convex colonies
44009	the predominant quinone is Q-8.

Metabolite utilization

@ref	Metabolite	Utilization activity	Kind of utilization tested
44009	casein	-	hydrolysis
44009	citrate	+	assimilation
44009	D-glucose	-	builds acid from
44009	D-glucose	-	builds gas from
44009	esculin	-	hydrolysis
44009	gelatin	+	hydrolysis
44009	nitrate	+	reduction
44009	starch	-	hydrolysis
44009	tween 80	-	hydrolysis
44009	tyrosine	+	assimilation
44009	urea	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite	Production
44009	35581 ChEBI	indole
44009	15138 ChEBI	sulfide

Enzymes

@ref	Value	Activity	Ec
44009	acid phosphatase	+	3.1.3.2
44009	alkaline phosphatase	-	3.1.3.1
44009	alpha-chymotrypsin	-	3.4.21.1
44009	alpha-fucosidase	-	3.2.1.51
44009	alpha-galactosidase	-	3.2.1.22
44009	alpha-glucosidase	+	3.2.1.20
44009	alpha-mannosidase	-	3.2.1.24
44009	beta-galactosidase	-	3.2.1.23
44009	beta-glucosidase	-	3.2.1.21
44009	beta-glucuronidase	-	3.2.1.31
44009	catalase	+	1.11.1.6
44009	cystine arylamidase	-	3.4.11.3
44009	cytochrome oxidase	+	1.9.3.1
44009	esterase (C 4)	+
44009	esterase Lipase (C 8)	+
44009	leucine arylamidase	-	3.4.11.1
44009	lipase (C 14)	-
44009	N-acetyl-beta-glucosaminidase	-	3.2.1.52
44009	naphthol-AS-BI-phosphohydrolase	+
44009	phosphatase	-
44009	trypsin	-	3.4.21.4
44009	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

44009

Fatty acid	Percentage
C18:1ω7c	63
C16:1ω7c	19.1
C16:0	10.1
C10:0 3OH	2.8
C12:0	1.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Condition	#Alkaline
#Environmental	#Aquatic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
44009	the alkaline water of Zab-szek soda pan	Kiskunsag National Park	Hungary	HUN	Europe
64979	alkaline water of a soda pan	Kiskunság National Park, Zab-szék (46° 50.190' N 19° 10.283'' E)	Hungary	HUN	Europe

Taxonmaps

69479

Global distribution of 16S sequence LN650603 (>99% sequence identity) for Nitrincola alkalilacustris subclade from Microbeatlas

Aquatic Samples	121
Soil Samples	63
Animal Samples	60
Plant Samples	17
Total Samples	261

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64979	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM897375v1 assembly for Nitrincola alkalilacustris NCAIM B.02612	contig	GCA_008973755	1571224.3	2890493618	1571224	73.64

Genome browser: GCA_008973755

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64979	Nitrincola sp. ZV-19 partial 16S rRNA gene, isolate ZV-19	LN650603	1473		1571224

GC content

@ref	GC-content (mol%)	Method
44009	54.5	sequence analysis
64979	54.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nitrincola tapanii sp. nov., a novel alkaliphilic bacterium from An Indian Soda Lake.	Joshi A, Thite S, Dhotre D, Moorthy M, Joseph N, Ramana VV, Shouche Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003883	2020
Phylogeny	Nitrincola alkalilacustris sp. nov. and Nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus Nitrincola.	Borsodi AK, Korponai K, Schumann P, Sproer C, Felfoldi T, Marialigeti K, Szili-Kovacs T, Toth E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002437	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#44009	Andrea K. Borsodi, Kristóf Korponai, Peter Schumann, Cathrin Spröer, Tamás Felföldi, Károly Márialigeti, Tibor Szili-Kovács, Erika Tóth: Nitrincola alkalilacustris sp. nov. and Nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus Nitrincola. IJSEM 67: 5159 - 5164 2017 ( DOI 10.1099/ijsem.0.002437 , PubMed 29056114 )
#64979	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29817
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nitrincola alkalilacustris (DSM 29817, NCAIM B.02612, NCAIM B 02612)

Name and taxonomic classification