Name: Nesterenkonia pannonica BV-35
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140783

Type strain:

Species: Nesterenkonia pannonica

Strain Designation: BV-35

Culture col. no.: NCAIM B 02606, DSM 29786

Strain history: <- A. K. Borsodi, Eötvös Loránd Univ. Budapest, Hungary; BV-35

NCBI tax ID(s): 1548602 (species)

SI-ID 400779

DSM 29786

Special Collections

Section

Nesterenkonia pannonica BV-35 is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from the water of the Böddi-szék soda pan.

colony-forming
Gram-negative
rod-shaped
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia pannonica**
	Full Scientific Name (LPSN)	Nesterenkonia pannonica Borsodi et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43561]	Gram stain	negative

[Ref.: #43561]	Cell length	0.6-0.8 µm

[Ref.: #43561]	Cell width	0.4-0.6 µm

[Ref.: #43561]	Cell shape	rod-shaped

[Ref.: #43561]	Motility	no

[Ref.: #43561]	Colony color	Orange
[Ref.: #43561]	Colony shape	circular
[Ref.: #43561]	Incubation period	1-2 days
[Ref.: #43561]	Cultivation medium used	modified R2A

Information on culture and growth conditions Culture and growth conditions

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Culture medium

modified R2A

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Culture medium growth

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #64899]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43561]	growth	10-40 °C
[Ref.: #43561]	optimum	25-28 °C
[Ref.: #64899]	growth	20 °C

	pH	Kind of pH		pH
[Ref.: #43561]			optimum	9-10
[Ref.: #43561]			growth	6-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43561]

Oxygen tolerance

obligate aerobe

[Ref.: #43561]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

[Ref.: #43561]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43561]		NaCl		growth	0-15	%(w/v)
[Ref.: #43561]		NaCl		optimum	5-7	%(w/v)

[Ref.: #64899]	Murein short key	A11.54
[Ref.: #64899]	Murein types	A4alpha L-Lys-L-Glu

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43561]	casein	-	hydrolysis
[Ref.: #43561]	citrate ChEBI	-	assimilation
[Ref.: #43561]	D-glucose ChEBI	-	builds acid from
[Ref.: #43561]	D-glucose ChEBI	-	builds gas from
[Ref.: #43561]	esculin ChEBI	+	hydrolysis
[Ref.: #43561]	gelatin ChEBI	-	hydrolysis
[Ref.: #43561]	glucose ChEBI	-	fermentation
[Ref.: #43561]	nitrate ChEBI	-	reduction
[Ref.: #43561]	starch ChEBI	-	hydrolysis
[Ref.: #43561]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43561]	urea ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43561]	acetoin ChEBI	no
[Ref.: #43561]	hydrogen sulfide ChEBI	no
[Ref.: #43561]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #43561]		acetoin ChEBI	-
[Ref.: #43561]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #43561]	acid phosphatase	-	3.1.3.2
[Ref.: #43561]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43561]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43561]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43561]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43561]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43561]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43561]	beta-galactosidase	-	3.2.1.23
[Ref.: #43561]	beta-glucosidase	-	3.2.1.21
[Ref.: #43561]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43561]	catalase	+	1.11.1.6
[Ref.: #43561]	cystine arylamidase	-	3.4.11.3
[Ref.: #43561]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43561]	esterase (C 4)	+
[Ref.: #43561]	esterase Lipase (C 8)	+
[Ref.: #43561]	leucine arylamidase	+	3.4.11.1
[Ref.: #43561]	lipase (C 14)	-
[Ref.: #43561]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43561]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43561]	trypsin	-	3.4.21.4
[Ref.: #43561]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43561]	Sample type/isolated from	the water of the Böddi-szék soda pan
[Ref.: #43561]	Geographic location (country and/or sea, region)	Ksikunság National Park
[Ref.: #43561]	Country	Hungary
[Ref.: #43561]	Country ISO 3 Code	HUN
[Ref.: #43561]	Continent	Europe
[Ref.: #43561]	Geographic location	46.7660°/19.1500°

[Ref.: #64899]	Sample type/isolated from	water of soda pan
[Ref.: #64899]	Geographic location (country and/or sea, region)	Kiskunság National Park, Böddi-szék soda pan (46° 46' N 019° 09' E)
[Ref.: #64899]	Country	Hungary
[Ref.: #64899]	Country ISO 3 Code	HUN
[Ref.: #64899]	Continent	Europe
[Ref.: #64899]	Geographic location	46.7667°/19.1500°
* marker position based on {}

Isolation sources categories

#Condition	#Alkaline	-
#Condition	#Saline	-
#Environmental	#Aquatic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN610504 (>99% sequence identity) for Nesterenkonia pannonica subclade from Microbeatlas

Aquatic Samples	64
Soil Samples	45
Animal Samples	468
Plant Samples	31
Total Samples	608

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64899]	Nesterenkonia sp. BV-35 partial 16S rRNA gene, strain BV-35	LN610504	1457	GenBank ENA	1548602 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nesterenkonia pannonica DSM 29786	GCA_019575955	complete	GenBank ENA	1548602 tax ID	*
[Ref.: #66792]	Nesterenkonia pannonica strain DSM 29786	1548602.3	complete	PATRIC	1548602 tax ID	*

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GC-content

65.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nesterenkonia pannonica strain DSM 29786 PATRIC: 1548602.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nesterenkonia pannonica DSM 29786 NCBI: GCA_019575955.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	83.33	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.804	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	84.394	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	75.922	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.299	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	71	no

Availability in culture collections External links

[Ref.: #64899]

Culture collection no.

NCAIM B 02606, DSM 29786

[Ref.: #96789]

SI-ID 400779

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium.	Borsodi AK, Szili-Kovacs T, Schumann P, Sproer C, Marialigeti K, Toth E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002263	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43561

Andrea K. Borsodi, Tibor Szili-Kovacs, Peter Schumann, Cathrin Spröer, Karoly Marialigeti, Erika Tóth: Nesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium. IJSEM 67: 4116 - 4120 2017 ( DOI 10.1099/ijsem.0.002263 , PubMed 28905704 )

#64899

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29786

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96789

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400779.1 )

* These data were automatically processed and therefore are not curated

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Nesterenkonia pannonica strain DSM 29786

Nesterenkonia pannonica DSM 29786

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