Strain identifier

BacDive ID: 140783

Type strain: Yes

Species: Nesterenkonia pannonica

Strain Designation: BV-35

Strain history: <- A. K. Borsodi, Eötvös Loránd Univ. Budapest, Hungary; BV-35

NCBI tax ID(s): 1548602 (species)

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General

@ref: 64899

BacDive-ID: 140783

DSM-Number: 29786

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, rod-shaped, colony-forming

description: Nesterenkonia pannonica BV-35 is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from the water of the Böddi-szék soda pan.

NCBI tax id

  • NCBI tax id: 1548602
  • Matching level: species

strain history

  • @ref: 64899
  • history: <- A. K. Borsodi, Eötvös Loránd Univ. Budapest, Hungary; BV-35

doi: 10.13145/bacdive140783.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Nesterenkonia
  • species: Nesterenkonia pannonica
  • full scientific name: Nesterenkonia pannonica Borsodi et al. 2017

@ref: 64899

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Nesterenkonia

species: Nesterenkonia pannonica

full scientific name: Nesterenkonia pannonica Borsodi et al. 2017

strain designation: BV-35

type strain: yes

Morphology

cell morphology

  • @ref: 43561
  • gram stain: negative
  • cell length: 0.6-0.8 µm
  • cell width: 0.4-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43561
  • colony color: Orange
  • colony shape: circular
  • incubation period: 1-2 days
  • medium used: modified R2A

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43561modified R2Ayes
64899R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperature
43561positivegrowth10-40
43561positiveoptimum25-28
64899positivegrowth20

culture pH

@refabilitytypepHPH range
43561positivegrowth6-12alkaliphile
43561positiveoptimum9-10

Physiology and metabolism

oxygen tolerance

  • @ref: 43561
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
43561no
69481no100

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43561NaClpositivegrowth0-15 %(w/v)
43561NaClpositiveoptimum5-7 %(w/v)moderately halophilic

murein

  • @ref: 64899
  • murein short key: A11.54
  • type: A4alpha L-Lys-L-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4356116947citrate-assimilation
4356117634D-glucose-builds acid from
4356117634D-glucose-builds gas from
4356117234glucose-fermentation
43561casein-hydrolysis
435615291gelatin-hydrolysis
4356128017starch-hydrolysis
4356153426tween 80-hydrolysis
4356117632nitrate-reduction
435614853esculin+hydrolysis
4356116199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4356115688acetoinno
4356116136hydrogen sulfideno
4356135581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
4356117234glucose-
4356115688acetoin-

enzymes

@refvalueactivityec
43561catalase+1.11.1.6
43561alkaline phosphatase+3.1.3.1
43561esterase (C 4)+
43561esterase Lipase (C 8)+
43561leucine arylamidase+3.4.11.1
43561naphthol-AS-BI-phosphohydrolase+
43561cytochrome oxidase-1.9.3.1
43561lipase (C 14)-
43561valine arylamidase-
43561cystine arylamidase-3.4.11.3
43561trypsin-3.4.21.4
43561alpha-chymotrypsin-3.4.21.1
43561acid phosphatase-3.1.3.2
43561alpha-galactosidase-3.2.1.22
43561beta-galactosidase-3.2.1.23
43561beta-glucuronidase-3.2.1.31
43561alpha-glucosidase-3.2.1.20
43561beta-glucosidase-3.2.1.21
43561N-acetyl-beta-glucosaminidase-3.2.1.52
43561alpha-mannosidase-3.2.1.24
43561alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43561the water of the Böddi-szék soda panKsikunság National ParkHungaryHUNEurope46.76619.15
64899water of soda panKiskunság National Park, Böddi-szék soda pan (46° 46' N 019° 09' E)HungaryHUNEurope46.766719.15

isolation source categories

Cat1Cat2
#Condition#Alkaline
#Condition#Saline
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_79323.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2198;97_40303;98_55298;99_79323&stattab=map
  • Last taxonomy: Nesterenkonia pannonica subclade
  • 16S sequence: LN610504
  • Sequence Identity:
  • Total samples: 608
  • soil counts: 45
  • aquatic counts: 64
  • animal counts: 468
  • plant counts: 31

Safety information

risk assessment

  • @ref: 64899
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64899
  • description: Nesterenkonia sp. BV-35 partial 16S rRNA gene, strain BV-35
  • accession: LN610504
  • length: 1457
  • database: nuccore
  • NCBI tax ID: 1548602

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nesterenkonia pannonica DSM 29786GCA_019575955completencbi1548602
66792Nesterenkonia pannonica strain DSM 297861548602.3completepatric1548602

GC content

  • @ref: 64899
  • GC-content: 65.4

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes83.33yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no93.804no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes84.394no
69480spore-formingspore-formingAbility to form endo- or exosporesno75.922yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.299yes
69480flagellatedmotile2+Ability to perform flagellated movementno71no

External links

@ref: 64899

culture collection no.: NCAIM B 02606, DSM 29786

straininfo link

  • @ref: 96789
  • straininfo: 400779

literature

  • topic: Phylogeny
  • Pubmed-ID: 28905704
  • title: Nesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium.
  • authors: Borsodi AK, Szili-Kovacs T, Schumann P, Sproer C, Marialigeti K, Toth E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002263
  • year: 2017
  • mesh: Alkalies, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hungary, Hydrogen-Ion Concentration, Micrococcaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43561Andrea K. Borsodi, Tibor Szili-Kovacs, Peter Schumann, Cathrin Spröer, Karoly Marialigeti, Erika TóthNesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium10.1099/ijsem.0.002263IJSEM 67: 4116-4120 201728905704
64899Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29786Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29786)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96789Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400779.1