Name: Polynucleobacter sphagniphilus MWH-Weng1-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140723

Type strain:

Species: Polynucleobacter sphagniphilus

Strain Designation: MWH-Weng1-1

Culture col. no.: CIP 111099, DSM 24018

Strain history: CIP <- 2016, DSMZ <- M.W. Hahn, Innsbruck Univ., Mondsee, Austria: strain MWH-Weng1-1

NCBI tax ID(s): 1743169 (species)

SI-ID 401906

DSM 24018, CIP 111099

Section

Polynucleobacter sphagniphilus MWH-Weng1-1 is an aerobe, chemoorganotroph, rod-shaped bacterium that forms circular colonies and was isolated from small acidic freshwater bog pond located at the edge of the Wenger Moor.

colony-forming
rod-shaped
aerobe
chemoorganotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter sphagniphilus**
	Full Scientific Name (LPSN)	Polynucleobacter sphagniphilus Hahn et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #123256]	Gram stain	negative

[Ref.: #123256]	Cell shape	rod-shaped
[Ref.: #43501]	Cell shape	rod-shaped

[Ref.: #123256]	Motility	no

[Ref.: #43501]	Cell length	0.6-1 µm

[Ref.: #43501]	Cell width	0.3-0.5 µm

[Ref.: #43501]	Colony color	Colourless
[Ref.: #43501]	Colony shape	circular
[Ref.: #43501]	Cultivation medium used	NSY agar plates

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43501]

Culture medium

NSY agar plates

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Culture medium growth

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #64880]

Culture medium composition

expand / minimize

[Ref.: #123256]

Culture medium

CIP Medium 566

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #43501]			growth	5-31 °C
[Ref.: #64880]			growth	25 °C

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Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43501]

Oxygen tolerance

aerobe

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Nutrition type

chemoorganotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43501]		NaCl		growth	0-0.4	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43501]	4-oxopentanoate ChEBI	+/-	assimilation
[Ref.: #43501]	acetate ChEBI	+	assimilation
[Ref.: #43501]	betaine ChEBI	-	assimilation
[Ref.: #43501]	citrate ChEBI	-	assimilation
[Ref.: #43501]	D-galactose ChEBI	-	assimilation
[Ref.: #43501]	D-galacturonic acid ChEBI	+	assimilation
[Ref.: #43501]	D-glucose ChEBI	-	assimilation
[Ref.: #43501]	D-lyxose ChEBI	-	assimilation
[Ref.: #43501]	fructose ChEBI	+/-	assimilation
[Ref.: #43501]	fumarate ChEBI	+	assimilation
[Ref.: #43501]	glycolate ChEBI	-	assimilation
[Ref.: #43501]	glyoxylate ChEBI	-	assimilation
[Ref.: #43501]	L-alanine ChEBI	+	assimilation
[Ref.: #43501]	L-asparagine ChEBI	-	assimilation
[Ref.: #43501]	L-aspartate ChEBI	-	assimilation
[Ref.: #43501]	L-fucose ChEBI	+/-	assimilation
[Ref.: #43501]	L-glutamate ChEBI	+	assimilation
[Ref.: #43501]	L-histidine ChEBI	-	assimilation
[Ref.: #43501]	L-leucine ChEBI	-	assimilation
[Ref.: #43501]	L-serine ChEBI	-	assimilation
[Ref.: #43501]	malate ChEBI	+	assimilation
[Ref.: #43501]	malonate ChEBI	+	assimilation
[Ref.: #43501]	mannose ChEBI	+/-	assimilation
[Ref.: #43501]	oxalate ChEBI	-	assimilation
[Ref.: #43501]	oxaloacetate ChEBI	-	assimilation
[Ref.: #43501]	propionate ChEBI	+/-	assimilation
[Ref.: #43501]	pyruvate ChEBI	+	assimilation
[Ref.: #43501]	sorbitol ChEBI	+/-	assimilation
[Ref.: #43501]	succinate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43501]	Sample type/isolated from	small acidic freshwater bog pond located at the edge of the Wenger Moor
[Ref.: #43501]	Geographic location (country and/or sea, region)	Salzburg
[Ref.: #43501]	Country	Austria
[Ref.: #43501]	Country ISO 3 Code	AUT
[Ref.: #43501]	Continent	Europe

[Ref.: #64880]	Sample type/isolated from	freshwater, pond in ombrotrophic bog ecosystem
[Ref.: #64880]	Geographic location (country and/or sea, region)	Wenger Moor, near lake Wallersee
[Ref.: #64880]	Country	Austria
[Ref.: #64880]	Country ISO 3 Code	AUT
[Ref.: #64880]	Continent	Europe

[Ref.: #123256]	Sample type/isolated from	Environment, Freshwater from pond in ombrotrophic bag
[Ref.: #123256]	Geographic location (country and/or sea, region)	Mondsee, Austria
[Ref.: #123256]	Country	Austria
[Ref.: #123256]	Country ISO 3 Code	AUT
[Ref.: #123256]	Continent	Europe
[Ref.: #123256]	Isolation date	2007-12-03
* marker position based on {}

Isolation sources categories

#Condition	#Acidic	-
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Pond (small)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64880]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123256]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43501]	16S rRNA gene sequence	MPIY01000000		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter sphagniphilus MWH-Weng1-1	GCA_001953355	contig	GenBank	1743169 tax ID	*
[Ref.: #66792]	Polynucleobacter sphagniphilus MWH-Weng1-1	2574179738	draft	JGI IMG/M	1743169 tax ID	*

[Ref.: #43501]	GC-content	45.6 mol%
[Ref.: #64880]	GC-content	45.6 mol%	sequence analysis

Availability in culture collections External links

[Ref.: #64880]

Culture collection no.

CIP 111099, DSM 24018

[Ref.: #96782]

SI-ID 401906

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter sphagniphilus sp. nov. a planktonic freshwater bacterium isolated from an acidic and humic freshwater habitat.	Hahn MW, Karbon G, Koll U, Schmidt J, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002096	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43501

Martin W. Hahn, Gerlinde Karbon, Ulrike Koll, Johanna Schmidt, Elke Lang: Polynucleobacter sphagniphilus sp. nov. a planktonic freshwater bacterium isolated from an acidic and humic freshwater habitat. IJSEM 67: 3261 - 3267 2017 ( DOI 10.1099/ijsem.0.002096 , PubMed 28829016 )

#64880

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24018

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96782

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401906.1 )

#123256 Collection of Institut Pasteur ; Curators of the CIP; CIP 111099
* These data were automatically processed and therefore are not curated

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