Actinokineospora acnipugnans (KEMB 9005-403, KACC 18904, JCM 31557)

Name: Actinokineospora acnipugnans (KEMB 9005-403, KACC 18904, JCM 31557)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140670

Type strain

Strain Designation R434

Culture col. no. KEMB 9005-403 KACC 18904 JCM 31557 R434 NEAU GQTH2-3 1 more

NCBI tax ID(s) 1813471

Special Collections JCM Literature strains

History JCM <-- J. Kim; Kyonggi Univ., South Korea; R434. DSMZ <- JCM; JCM 31557

Links

Actinokineospora acnipugnans R434 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from reclaimed grassland.

spore-forming Gram-positive rod-shaped aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 285351

DSM 118753,JCM 31557

@ref 20215

Last LPSN update 2026-05-29 08:39:46

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinokineospora

Species Actinokineospora acnipugnans

Full scientific name Actinokineospora acnipugnans Dahal et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
43434	positive	rod-shaped

Colony morphology

43434

Colony coloryellow, white

Multimedia

@ref:	126077
multimedia content:	DSM_118753.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_118753.jpg
caption:	Medium 554 28°C
intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
43434	ISP
43434	Reasoner's 2A agar (R2A)
43434	Trypticase Soy Agar (TSA)
126077	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
126077	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
126077	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
43434	positive	growth	20-45
43434	positive	optimum	25-35
67770	positive	growth	28
126077	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
43434	positive	optimum	7.5-9
43434	positive	growth	6.5-10.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

43434

Oxygen toleranceaerobe

Spore formation

@ref	Type of spore	Spore formation
43434	spore

Halophily

@ref	Halophily level	Salt	Growth	Tested relation	Concentration
43434		NaCl	positive	growth	3 %(w/v)
43434	non-halophilic	NaCl	positive	optimum	0 %(w/v)

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43434	85146 ChEBI	carboxymethylcellulose	+	hydrolysis
43434		casein	+	hydrolysis
43434	62968 ChEBI	cellulose	+	carbon source
43434	17029 ChEBI	chitin	-	hydrolysis
43434	12936 ChEBI	D-galactose	+	carbon source
43434	17634 ChEBI	D-glucose	+	carbon source
43434	16313 ChEBI	D-proline	-	nitrogen source
43434	16988 ChEBI	D-ribose	-	carbon source
43434	27477 ChEBI	D-valine	+	nitrogen source
43434	65327 ChEBI	D-xylose	+	carbon source
43434	5291 ChEBI	gelatin	+	hydrolysis
43434	17368 ChEBI	hypoxanthine	-	hydrolysis
43434	30849 ChEBI	L-arabinose	+	carbon source
43434	16467 ChEBI	L-arginine	-	nitrogen source
43434	62345 ChEBI	L-rhamnose	-	carbon source
43434	17115 ChEBI	L-serine	+	nitrogen source
43434	17632 ChEBI	nitrate	+	reduction
43434	16634 ChEBI	raffinose	+	carbon source
43434	28017 ChEBI	starch	+	hydrolysis
43434	17992 ChEBI	sucrose	+	carbon source
43434	53423 ChEBI	tween 40	-	hydrolysis
43434	53425 ChEBI	tween 60	-	hydrolysis
43434	53426 ChEBI	tween 80	-	hydrolysis
43434	18186 ChEBI	tyrosine	-	hydrolysis
43434	15318 ChEBI	xanthine	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43434	15688 ChEBI	acetoin
43434	16136 ChEBI	hydrogen sulfide

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
43434	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
43434	catalase	+	1.11.1.6
43434	cytochrome oxidase	-	1.9.3.1
43434	DNase	+
43434	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

@ref

43434

Fatty acid	Percentage
C16:0 iso	55.8
C16:1 iso H	16.7
C17:1ω6c	10.2
C16:1ω7c / C16:1ω6c	3.9
C15:0 iso	3.8
C14:0 iso	2.2
C17:0 anteiso	1.8
C17:0 iso	1.1
C17:1ω8c	1
iso-C17:1ω9c / C16:010-methyl	0.8
C16:0	0.7
C15:1 iso G	0.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Grassland

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
43434	reclaimed grassland	Hwaseong, South Korea	Republic of Korea	KOR	Asia	37.2694	126.761 37.2694/126.761
67770	Soil	Hwaseong, Gyeonggi-do	Republic of Korea	KOR	Asia
126077	soil of grassland	Hwaseong	Republic of Korea	KOR	Asia

Taxonmaps

69479

Global distribution of 16S sequence KU881048 (>99% sequence identity) for Actinokineospora from Microbeatlas

Aquatic Samples	16
Soil Samples	63
Animal Samples	13
Plant Samples	25
Total Samples	117

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
126077	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Database
43434	Actinokineospora acnipugnans strain R434 16S ribosomal RNA gene, partial sequence	KU881048

GC content

@ref	GC-content (mol%)	Method
67770	71.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics.	Dahal RH, Shim DS, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002078	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43434	Ram Hari Dahal, Dong Seop Shim, Jaisoo Kim: Description of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics. IJSEM 67: 3043 - 3049 2017 ( DOI 10.1099/ijsem.0.002078 , PubMed 28820111 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126077	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 118753
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinokineospora acnipugnans (KEMB 9005-403, KACC 18904, JCM 31557)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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