Name: Actinokineospora acnipugnans R434
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140670

Type strain:

Species: Actinokineospora acnipugnans

Strain Designation: R434

Culture col. no.: KEMB 9005-403, KACC 18904, JCM 31557

Strain history: J. Kim; Kyonggi Univ., South Korea; R434.

NCBI tax ID(s): 1813471 (species)

Section

Actinokineospora acnipugnans R434 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from reclaimed grassland.

Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinokineospora*
	Species	**Actinokineospora acnipugnans**
	Full Scientific Name (PNU)	Actinokineospora acnipugnans Dahal et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43434]	Gram stain	positive
[Ref.: #43434]	Cell shape	rod-shaped
[Ref.: #43434]	Motility	no

[Ref.: #43434]

Colony color

yellow, white

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43434]

Culture medium

ISP

[Ref.: #43434]

Culture medium growth

[Ref.: #43434]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43434]

Culture medium growth

[Ref.: #43434]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #43434]

Culture medium growth

		Temperature
[Ref.: #43434]	growth	20-45 °C
[Ref.: #43434]	optimum	25-35 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #43434]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43434]			optimum	7.5-9
[Ref.: #43434]			growth	6.5-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43434]

Oxygen tolerance

aerobe

[Ref.: #43434]	Type of spore	spore
[Ref.: #43434]	Ability of spore formation	yes

[Ref.: #43434]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43434]		NaCl		growth	3	%(w/v)
[Ref.: #43434]		NaCl		optimum	0	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43434]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #43434]	casein	+	hydrolysis
[Ref.: #43434]	cellulose ChEBI	+	carbon source
[Ref.: #43434]	chitin ChEBI	-	hydrolysis
[Ref.: #43434]	D-galactose ChEBI	+	carbon source
[Ref.: #43434]	D-glucose ChEBI	+	carbon source
[Ref.: #43434]	D-proline ChEBI	-	nitrogen source
[Ref.: #43434]	D-ribose ChEBI	-	carbon source
[Ref.: #43434]	D-valine ChEBI	+	nitrogen source
[Ref.: #43434]	D-xylose ChEBI	+	carbon source
[Ref.: #43434]	gelatin ChEBI	+	hydrolysis
[Ref.: #43434]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43434]	L-arabinose ChEBI	+	carbon source
[Ref.: #43434]	L-arginine ChEBI	-	nitrogen source
[Ref.: #43434]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43434]	L-serine ChEBI	+	nitrogen source
[Ref.: #43434]	nitrate ChEBI	+	reduction
[Ref.: #43434]	raffinose ChEBI	+	carbon source
[Ref.: #43434]	starch ChEBI	+	hydrolysis
[Ref.: #43434]	sucrose ChEBI	+	carbon source
[Ref.: #43434]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43434]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43434]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43434]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43434]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #43434]		acetoin ChEBI
[Ref.: #43434]		hydrogen sulfide ChEBI

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43434]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #43434]	catalase	+	1.11.1.6
[Ref.: #43434]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43434]	DNase	+
[Ref.: #43434]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43434]

Fatty acid	Percentage
C16:0 iso	55.8
C16:1 iso H	16.7
C17:1ω6c	10.2
C16:1ω7c / C16:1ω6c	3.9
C15:0 iso	3.8
C14:0 iso	2.2
C17:0 anteiso	1.8
C17:0 iso	1.1
C17:1ω8c	1
iso-C17:1ω9c / C16:010-methyl	0.8
C16:0	0.7
C15:1 iso G	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43434]	Sample type/isolated from	reclaimed grassland
[Ref.: #43434]	Geographic location (country and/or sea, region)	Hwaseong, South Korea
[Ref.: #43434]	Country	Republic of Korea
[Ref.: #43434]	Country ISO 3 Code	KOR
[Ref.: #43434]	Continent	Asia
[Ref.: #43434]	Geographic location	37.2694°/126.7610°

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Hwaseong, Gyeonggi-do
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Grassland

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU881048 (>99% sequence identity) for Actinokineospora from Microbeatlas

Aquatic Samples	16
Soil Samples	63
Animal Samples	13
Plant Samples	25
Total Samples	117

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43434]	16S rRNA gene sequence	KU881048		GenBank

[Ref.: #67770]

GC-content

71.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43434]

Culture collection no.

KEMB 9005-403, KACC 18904, JCM 31557

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics.	Dahal RH, Shim DS, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002078	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43434

Ram Hari Dahal, Dong Seop Shim, Jaisoo Kim: Description of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics. IJSEM 67: 3043 - 3049 2017 ( DOI 10.1099/ijsem.0.002078 , PubMed 28820111 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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