Cumulibacter manganitolerans 2-36 | Type strain | CCTCC AA 2016026, DSM 103787

Caption:

Medium 830 28°C

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Information on culture and growth conditions Culture and growth conditions

Culture medium

GYM

Culture medium growth

Culture medium

ISP2

Culture medium growth

Culture medium

LB (Luria-Bertani) MEDIUM

Culture medium growth

Culture medium

Culture medium growth

Culture medium

Reasoner's 2A agar (R2A)

Culture medium growth

Culture medium

TSB

Culture medium growth

Culture medium

R2A MEDIUM (DSMZ Medium 830)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

GEO-MEDIUM (DSMZ Medium 714)

Culture medium growth

Culture medium link

Culture medium composition

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

Culture medium growth

Culture medium link

Culture medium composition

		Temperature
[Ref.: #43433]	growth	15-37 °C
[Ref.: #43433]	optimum	28 °C
[Ref.: #64868]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #43433]			optimum	5-8
[Ref.: #43433]			growth	4-10

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

obligate aerobe

Nutrition type

chemoheterotroph

[Ref.: #43433]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %94

What are isolation sources categories?

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43433]		NaCl		growth	6	%(w/v)
[Ref.: #43433]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43433]	adipate ChEBI	+	assimilation
[Ref.: #43433]	D-glucose ChEBI	+	assimilation
[Ref.: #43433]	D-mannitol ChEBI	-	assimilation
[Ref.: #43433]	D-mannose ChEBI	+	assimilation
[Ref.: #43433]	esculin ChEBI	-	hydrolysis
[Ref.: #43433]	gelatin ChEBI	-	hydrolysis
[Ref.: #43433]	L-arabinose ChEBI	-	assimilation
[Ref.: #43433]	L-arginine ChEBI	-	hydrolysis
[Ref.: #43433]	L-tryptophan ChEBI	-	hydrolysis
[Ref.: #43433]	malate ChEBI	+	assimilation
[Ref.: #43433]	maltose ChEBI	-	assimilation
[Ref.: #43433]	nitrate ChEBI	+	reduction
[Ref.: #43433]	phenylacetate ChEBI	+	assimilation
[Ref.: #43433]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43433]	starch ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43433]	acid phosphatase	+	3.1.3.2
[Ref.: #43433]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43433]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43433]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43433]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43433]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43433]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43433]	beta-galactosidase	-	3.2.1.23
[Ref.: #43433]	beta-glucosidase	-	3.2.1.21
[Ref.: #43433]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43433]	cystine arylamidase	+	3.4.11.3
[Ref.: #43433]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43433]	esterase (C 4)	+
[Ref.: #43433]	esterase Lipase (C 8)	+
[Ref.: #43433]	leucine arylamidase	+	3.4.11.1
[Ref.: #43433]	lipase (C 14)	-
[Ref.: #43433]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43433]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43433]	trypsin	-	3.4.21.4
[Ref.: #43433]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43433]	Sample type/isolated from	sludge of a seage outlet in Dalong manganese mine
[Ref.: #43433]	Geographic location (country and/or sea, region)	Dalong manganese mine, Tongren, Guizhou province, PR China
[Ref.: #43433]	Country	China
[Ref.: #43433]	Country ISO 3 Code	CHN
[Ref.: #43433]	Continent	Asia

[Ref.: #64868]	Sample type/isolated from	sludge of a sewage outlet of a manganese mining factory
[Ref.: #64868]	Geographic location (country and/or sea, region)	Guizhou Province, Tongren, Dalong Manganese Mine (27° 43' 8'' N, 108° 31' 42'' E)
[Ref.: #64868]	Country	China
[Ref.: #64868]	Country ISO 3 Code	CHN
[Ref.: #64868]	Continent	Asia
[Ref.: #64868]	Geographic location	27.7189°/108.5280°
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Sewage sludge
#Engineered	#Other	#Mine
#Engineered	#Contamination	#Heavy metal

[Ref.: #69479]

Global distribution of 16S sequence KX602199 (>99% sequence identity) for Actinomycetia from Microbeatlas

Aquatic Samples	250
Soil Samples	380
Animal Samples	1178
Plant Samples	32
Total Samples	1840

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64868]	Cumulibacter manganitolerans strain 2-36 16S ribosomal RNA gene, partial sequence	KX602199	1483	GenBank ENA	1884992 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cumulibacter manganitolerans 2-36	GCA_009602465	scaffold	GenBank ENA	1884992 tax ID	*
[Ref.: #66792]	Cumulibacter manganitolerans 2-36	2887435738	draft	JGI IMG/M	1884992 tax ID	*

GC-content

70.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cumulibacter manganitolerans 2-36 NCBI: GCA_009602465.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	94	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cumulibacter manganitolerans 2-36 NCBI: GCA_009602465.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.602	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.761	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.161	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	69.034	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93.6	yes

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