Strain identifier

BacDive ID: 140670

Type strain: Yes

Species: Actinokineospora acnipugnans

Strain Designation: R434

Strain history: J. Kim; Kyonggi Univ., South Korea; R434.

NCBI tax ID(s): 1813471 (species)

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General

@ref: 43434

BacDive-ID: 140670

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped

description: Actinokineospora acnipugnans R434 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from reclaimed grassland.

NCBI tax id

  • NCBI tax id: 1813471
  • Matching level: species

strain history

  • @ref: 67770
  • history: J. Kim; Kyonggi Univ., South Korea; R434.

doi: 10.13145/bacdive140670.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinokineospora
  • species: Actinokineospora acnipugnans
  • full scientific name: Actinokineospora acnipugnans Dahal et al. 2017

@ref: 43434

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Actinokineospora

species: Actinokineospora acnipugnans

strain designation: R434

type strain: yes

Morphology

cell morphology

  • @ref: 43434
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43434
  • colony color: yellow, white

Culture and growth conditions

culture medium

@refnamegrowth
43434ISPyes
43434Reasoner's 2A agar (R2A)yes
43434Trypticase Soy Agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
43434positivegrowth20-45
43434positiveoptimum25-35mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43434positivegrowth6.5-10.5alkaliphile
43434positiveoptimum7.5-9

Physiology and metabolism

oxygen tolerance

  • @ref: 43434
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43434
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43434NaClpositivegrowth3 %(w/v)
43434NaClpositiveoptimum0 %(w/v)non-halophilic

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4343416988D-ribose-carbon source
4343462345L-rhamnose-carbon source
4343417029chitin-hydrolysis
4343417368hypoxanthine-hydrolysis
4343453423tween 40-hydrolysis
4343453425tween 60-hydrolysis
4343453426tween 80-hydrolysis
4343418186tyrosine-hydrolysis
4343415318xanthine-hydrolysis
4343416313D-proline-nitrogen source
4343416467L-arginine-nitrogen source
4343462968cellulose+carbon source
4343412936D-galactose+carbon source
4343417634D-glucose+carbon source
4343465327D-xylose+carbon source
4343430849L-arabinose+carbon source
4343416634raffinose+carbon source
4343417992sucrose+carbon source
4343485146carboxymethylcellulose+hydrolysis
43434casein+hydrolysis
434345291gelatin+hydrolysis
4343428017starch+hydrolysis
4343427477D-valine+nitrogen source
4343417115L-serine+nitrogen source
4343417632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
4343415688acetoinno
4343416136hydrogen sulfideno

metabolite tests

  • @ref: 43434
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
43434catalase+1.11.1.6
43434cytochrome oxidase-1.9.3.1
43434DNase+
43434urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43434C17:0 anteiso1.8
    43434C16:00.7
    43434C16:1ω7c / C16:1ω6c3.9
    43434C17:1ω6c10.2
    43434C17:1ω8c1
    43434C14:0 iso2.2
    43434C15:0 iso3.8
    43434C15:1 iso G0.5
    43434C16:0 iso55.8
    43434C16:1 iso H16.7
    43434C17:0 iso1.1
    43434iso-C17:1ω9c / C16:010-methyl0.8
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43434reclaimed grasslandHwaseong, South KoreaRepublic of KoreaKORAsia37.2694126.761
67770SoilHwaseong, Gyeonggi-doRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Grassland

taxonmaps

  • @ref: 69479
  • File name: preview.99_38451.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_394;97_640;98_11399;99_38451&stattab=map
  • Last taxonomy: Actinokineospora
  • 16S sequence: KU881048
  • Sequence Identity:
  • Total samples: 117
  • soil counts: 63
  • aquatic counts: 16
  • animal counts: 13
  • plant counts: 25

Sequence information

16S sequences

  • @ref: 43434
  • description: 16S rRNA gene sequence
  • accession: KU881048
  • database: nuccore

GC content

  • @ref: 67770
  • GC-content: 71.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43434

culture collection no.: KEMB 9005-403, KACC 18904, JCM 31557

literature

  • topic: Phylogeny
  • Pubmed-ID: 28820111
  • title: Description of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics.
  • authors: Dahal RH, Shim DS, Kim J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002078
  • year: 2017
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cosmetics, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Grassland, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43434Ram Hari Dahal, Dong Seop Shim, Jaisoo KimDescription of Actinokineospora acnipugnans sp. nov., an actinomycete isolated from soil, showing potential uses in cosmetics10.1099/ijsem.0.002078IJSEM 67: 3043-3049 201728820111
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/