Name: Kingella negevensis CSUR P957
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140547

Type strain:

Species: Kingella negevensis

Culture col. no.: CSUR P957, CCUG 69806, SCH Sch538

NCBI tax ID(s): 1522312 (species)

SI-ID 408919

CCUG 69806

Special Collections

Section

Kingella negevensis CSUR P957 is an aerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from oropharynx of healthy children in Israel and Switzerland.

coccus-shaped
colony-forming
Gram-negative
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Neisseriales*
	Family	*Neisseriaceae*
	Genus	*Kingella*
	Species	**Kingella negevensis**
	Full Scientific Name (LPSN)	Kingella negevensis El Houmami et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43336]	Gram stain	negative

[Ref.: #43336]	Cell length	1-1.7 µm

[Ref.: #43336]	Cell width	0.7-1.2 µm

[Ref.: #43336]	Cell shape	coccus-shaped

[Ref.: #43336]	Motility	no

[Ref.: #43336]	Type of hemolysis	beta
[Ref.: #43336]	Hemolysis ability	1
[Ref.: #43336]	Colony size	0.5-1 mm
[Ref.: #43336]	Colony color	pale yellow
[Ref.: #43336]	Colony shape	circular
[Ref.: #43336]	Incubation period	1 day
[Ref.: #43336]	Cultivation medium used	Columbia agar

[Ref.: #63674]	Incubation period	1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43336]

Culture medium

Columbia agar

[Ref.: #43336]

Culture medium growth

[Ref.: #43336]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #63674]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43336]	Oxygen tolerance	aerobe
[Ref.: #43336]	Oxygen tolerance	facultative anaerobe
[Ref.: #63674]	Oxygen tolerance	microaerophile

[Ref.: #43336]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43336]	fructose ChEBI	-	builds acid from
[Ref.: #43336]	glucose ChEBI	+	builds acid from
[Ref.: #43336]	maltose hydrate	-	builds acid from
[Ref.: #43336]	sucrose ChEBI	-	builds acid from

	Metabolite production	Metabolite	Production
[Ref.: #43336]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43336]	acid phosphatase	+	3.1.3.2
[Ref.: #43336]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43336]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43336]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43336]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43336]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43336]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43336]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43336]	beta-galactosidase	-	3.2.1.23
[Ref.: #43336]	beta-glucosidase	-	3.2.1.21
[Ref.: #43336]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43336]	catalase	-	1.11.1.6
[Ref.: #43336]	cystine arylamidase	-	3.4.11.3
[Ref.: #43336]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43336]	esterase	-
[Ref.: #43336]	esterase Lipase (C 8)	-
[Ref.: #43336]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #43336]	leucine arylamidase	+	3.4.11.1
[Ref.: #43336]	lipase	-
[Ref.: #43336]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43336]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #43336]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43336]	proline-arylamidase	-	3.4.11.5
[Ref.: #43336]	trypsin	-	3.4.21.4
[Ref.: #43336]	urease	-	3.5.1.5
[Ref.: #43336]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	ubiquinone biosynthesis	100	7 of 7
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	denitrification	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	vitamin B6 metabolism	81.82	9 of 11
[Ref.: #66794]	methylglyoxal degradation	80	4 of 5
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	lactate fermentation	75	3 of 4
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	glycolysis	58.82	10 of 17
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	purine metabolism	56.38	53 of 94
[Ref.: #66794]	degradation of sugar alcohols	56.25	9 of 16
[Ref.: #66794]	CO2 fixation in Crenarchaeota	55.56	5 of 9
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	leucine metabolism	53.85	7 of 13
[Ref.: #66794]	non-pathway related	52.63	20 of 38
[Ref.: #66794]	oxidative phosphorylation	50.55	46 of 91
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	Entner Doudoroff pathway	50	5 of 10
[Ref.: #66794]	tryptophan metabolism	50	19 of 38
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	tyrosine metabolism	50	7 of 14
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	glutamate and glutamine metabolism	50	14 of 28
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	alanine metabolism	48.28	14 of 29
[Ref.: #66794]	lysine metabolism	42.86	18 of 42
[Ref.: #66794]	glutathione metabolism	42.86	6 of 14
[Ref.: #66794]	pyrimidine metabolism	42.22	19 of 45
[Ref.: #66794]	lipid metabolism	41.94	13 of 31
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	hydrogen production	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	citric acid cycle	35.71	5 of 14
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	propionate fermentation	30	3 of 10
[Ref.: #66794]	arginine metabolism	29.17	7 of 24
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	cholesterol biosynthesis	27.27	3 of 11
[Ref.: #66794]	proline metabolism	27.27	3 of 11
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	glycogen biosynthesis	25	1 of 4
[Ref.: #66794]	histidine metabolism	24.14	7 of 29
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	urea cycle	23.08	3 of 13
[Ref.: #66794]	carotenoid biosynthesis	22.73	5 of 22
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	degradation of pentoses	21.43	6 of 28
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43336]	Sample type/isolated from	oropharynx of healthy children in Israel and Switzerland
[Ref.: #43336]	Country	Israel
[Ref.: #43336]	Country ISO 3 Code	ISR
[Ref.: #43336]	Continent	Asia
[Ref.: #43336]	Enrichment culture	BAV medium

[Ref.: #63674]	Sample type/isolated from	oropharynx of healthy children
[Ref.: #63674]	Sampling date	2014-07-01
[Ref.: #63674]	Geographic location (country and/or sea, region)	Negev
[Ref.: #63674]	Country	Israel
[Ref.: #63674]	Country ISO 3 Code	ISR
[Ref.: #63674]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Human	#Child
#Host Body-Site	#Oral cavity and airways	#Throat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LK985395 (>99% sequence identity) for Kingella negevensis subclade from Microbeatlas

Aquatic Samples	502
Soil Samples	29
Animal Samples	2587
Plant Samples	6
Total Samples	3124

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43336]	Kingella sp. Sch538 partial 16S rRNA gene, isolate Sch538	LK985395		GenBank ENA

[Ref.: #43336]

GC-content

45.7 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #43336]

Culture collection no.

CSUR P957, CCUG 69806, SCH Sch538

[Ref.: #96740]

SI-ID 408919

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population.	El Houmami N, Bakour S, Bzdrenga J, Rathored J, Seligmann H, Robert C, Armstrong N, Schrenzel J, Raoult D, Yagupsky P, Fournier PE	Int J Syst Evol Microbiol	10.1099/ijsem.0.001957	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43336

Nawal El Houmami, Sofiane Bakour, Janek Bzdrenga, Jaishiram Rathored, Hervé Seligmann, Catherine Robert, Nicholas Armstrong, Jacques Schrenzel, Didier Raoult, Pablo Yagupsky, Pierre-Edouard Fournier: Isolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population. IJSEM 67: 2370 - 2376 2017 ( DOI 10.1099/ijsem.0.001957 , PubMed 28699877 )

#63674 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 69806

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96740

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408919.1 )

* These data were automatically processed and therefore are not curated

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