Strain identifier

BacDive ID: 140547

Type strain: Yes

Species: Kingella negevensis

NCBI tax ID(s): 1522312 (species)

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General

@ref: 43336

BacDive-ID: 140547

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Kingella negevensis CSUR P957 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from oropharynx of healthy children in Israel and Switzerland.

NCBI tax id

  • NCBI tax id: 1522312
  • Matching level: species

doi: 10.13145/bacdive140547.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Kingella
  • species: Kingella negevensis
  • full scientific name: Kingella negevensis El Houmami et al. 2017

@ref: 43336

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Kingella

species: Kingella negevensis

type strain: yes

Morphology

cell morphology

  • @ref: 43336
  • gram stain: negative
  • cell length: 1-1.7 µm
  • cell width: 0.7-1.2 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilitycolony sizecolony colorcolony shapeincubation periodmedium used
43336beta10.5-1 mmpale yellowcircular1 dayColumbia agar
636741 day

Culture and growth conditions

culture medium

  • @ref: 43336
  • name: Columbia agar
  • growth: yes
  • composition: 5% sheep blood-enriched

culture temp

  • @ref: 63674
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43336aerobe
43336facultative anaerobe
63674microaerophile

spore formation

  • @ref: 43336
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4333617234glucose+builds acid from
43336maltose hydrate-builds acid from
4333628757fructose-builds acid from
4333617992sucrose-builds acid from

metabolite production

  • @ref: 43336
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43336cytochrome oxidase+1.9.3.1
43336urease-3.5.1.5
43336catalase-1.11.1.6
43336alkaline phosphatase-3.1.3.1
43336proline-arylamidase-3.4.11.5
43336gamma-glutamyltransferase-2.3.2.2
43336ornithine decarboxylase-4.1.1.17
43336alkaline phosphatase+3.1.3.1
43336leucine arylamidase+3.4.11.1
43336acid phosphatase+3.1.3.2
43336esterase-
43336esterase Lipase (C 8)-
43336lipase-
43336beta-glucosidase-3.2.1.21
43336beta-galactosidase-3.2.1.23
43336alpha-mannosidase-3.2.1.24
43336alpha-fucosidase-3.2.1.51
43336cystine arylamidase-3.4.11.3
43336valine arylamidase-
43336trypsin-3.4.21.4
43336alpha-chymotrypsin-3.4.21.1
43336alpha-glucosidase-3.2.1.20
43336alpha-galactosidase-3.2.1.22
43336beta-glucuronidase-3.2.1.31
43336naphthol-AS-BI-phosphohydrolase-
43336N-acetyl-beta-glucosaminidase-3.2.1.52

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment culturesampling dategeographic location
43336oropharynx of healthy children in Israel and SwitzerlandIsraelISRAsiaBAV medium
63674oropharynx of healthy childrenIsraelISRAsia2014-07-01Negev

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body-Site#Oral cavity and airways#Throat

taxonmaps

  • @ref: 69479
  • File name: preview.99_3726.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_1209;97_1433;98_2845;99_3726&stattab=map
  • Last taxonomy: Kingella negevensis subclade
  • 16S sequence: LK985395
  • Sequence Identity:
  • Total samples: 3124
  • soil counts: 29
  • aquatic counts: 502
  • animal counts: 2587
  • plant counts: 6

Sequence information

16S sequences

  • @ref: 43336
  • description: 16S rRNA gene sequence
  • accession: LK985395
  • database: nuccore

GC content

  • @ref: 43336
  • GC-content: 45.7
  • method: genome sequence analysis

External links

@ref: 43336

culture collection no.: CSUR P957, CCUG 69806, SCH Sch538

straininfo link

  • @ref: 96740
  • straininfo: 408919

literature

  • topic: Phylogeny
  • Pubmed-ID: 28699877
  • title: Isolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population.
  • authors: El Houmami N, Bakour S, Bzdrenga J, Rathored J, Seligmann H, Robert C, Armstrong N, Schrenzel J, Raoult D, Yagupsky P, Fournier PE
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001957
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Child, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Humans, Israel, Kingella/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Oropharynx/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Switzerland
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43336Nawal El Houmami, Sofiane Bakour, Janek Bzdrenga, Jaishiram Rathored, Hervé Seligmann, Catherine Robert, Nicholas Armstrong, Jacques Schrenzel, Didier Raoult, Pablo Yagupsky, Pierre-Edouard FournierIsolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population10.1099/ijsem.0.001957IJSEM 67: 2370-2376 201728699877
63674Curators of the CCUGhttps://www.ccug.se/strain?id=69806Culture Collection University of Gothenburg (CCUG) (CCUG 69806)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
96740Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408919.1