Name: Nocardia heshunensis DSM 46764
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140373

Type strain:

Species: Nocardia heshunensis

Culture col. no.: DSM 46764, JCM 30085, CFH S0067

Strain history: W.-J. Li CFH S0067.

NCBI tax ID(s): 2030913 (species)

SI-ID 400258

DSM 46764

Special Collections

Section

Nocardia heshunensis DSM 46764 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Nocardia*
	Species	**Nocardia heshunensis**
	Full Scientific Name (LPSN)	Nocardia heshunensis Huang et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43899]	Gram stain	positive

[Ref.: #43899]	Cell shape	rod-shaped
[Ref.: #43899]	Cell shape	coccus-shaped

[Ref.: #43899]	Motility	no

[Ref.: #43899]	Colony color	light-orange
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	ISP 2 agar

[Ref.: #43899]	Colony color	light yellowish pink
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	ISP 3 agar

[Ref.: #43899]	Colony color	colourless
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	ISP 5 agar

[Ref.: #43899]	Colony color	light orange
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	Potato-dextrose agar

[Ref.: #43899]	Colony color	yellowish pink
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	ISP 4 agar

[Ref.: #43899]	Colony color	light yellowish pink
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	Czapek's agar

[Ref.: #43899]	Colony color	colourless
[Ref.: #43899]	Incubation period	7 days
[Ref.: #43899]	Cultivation medium used	Nutrient agar

[Ref.: #43899]	Pigment production	no
[Ref.: #43899]	Pigment name	diffusible pigments

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Czapek's agar

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Culture medium growth

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Culture medium

ISP 2 agar

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Culture medium growth

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Culture medium

ISP 3 agar

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Culture medium growth

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Culture medium

ISP 4 agar

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Culture medium growth

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Culture medium

ISP 5 agar

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Culture medium growth

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Culture medium

Potato-dextrose agar

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Culture medium growth

[Ref.: #42980]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #42980]

Culture medium composition

expand / minimize

[Ref.: #43899]

Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #42980]	growth	28 °C
[Ref.: #43899]	growth	4-50 °C
[Ref.: #43899]	optimum	4-37 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #43899]			optimum	4.0-7.0
[Ref.: #43899]			growth	4.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43899]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43899]		NaCl		growth	0-4	%(w/v)
[Ref.: #43899]		NaCl		optimum	0-2	%(w/v)

[Ref.: #43899]	Observation	quinone MK-8
[Ref.: #67770]	Observation	quinones: MK-8(H₄, ω-cycl)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43899]	cellobiose ChEBI	+	carbon source
[Ref.: #43899]	cellulose ChEBI	-	degradation
[Ref.: #43899]	D-fructose ChEBI	+	carbon source
[Ref.: #43899]	D-galactose ChEBI	+	carbon source
[Ref.: #43899]	D-glucose ChEBI	+	carbon source
[Ref.: #43899]	D-mannitol ChEBI	+	carbon source
[Ref.: #43899]	D-mannose ChEBI	+	carbon source
[Ref.: #43899]	D-trehalose ChEBI	-	carbon source
[Ref.: #43899]	D-xylose ChEBI	-	carbon source
[Ref.: #43899]	gelatin ChEBI	-	degradation
[Ref.: #43899]	glycerol ChEBI	+	carbon source
[Ref.: #43899]	glycine ChEBI	+	nitrogen source
[Ref.: #43899]	hypoxanthine ChEBI	+	nitrogen source
[Ref.: #43899]	L-alanine 4-nitroanilide	+	nitrogen source
[Ref.: #43899]	L-arabinose ChEBI	+	carbon source
[Ref.: #43899]	L-arginine ChEBI	-	nitrogen source
[Ref.: #43899]	L-asparagine ChEBI	-	nitrogen source
[Ref.: #43899]	L-cystine ChEBI	-	nitrogen source
[Ref.: #43899]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #43899]	L-lysine ChEBI	-	nitrogen source
[Ref.: #43899]	L-ornithine ChEBI	+	nitrogen source
[Ref.: #43899]	L-phenylalanine ChEBI	-	nitrogen source
[Ref.: #43899]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43899]	L-serine ChEBI	+	nitrogen source
[Ref.: #43899]	L-threonine ChEBI	-	nitrogen source
[Ref.: #43899]	L-tyrosine ChEBI	+	nitrogen source
[Ref.: #43899]	L-valine ChEBI	+	nitrogen source
[Ref.: #43899]	lactose ChEBI	-	carbon source
[Ref.: #43899]	maltose ChEBI	+	carbon source
[Ref.: #43899]	myo-inositol ChEBI	+	carbon source
[Ref.: #43899]	nitrate ChEBI	-	reduction
[Ref.: #43899]	raffinose ChEBI	-	carbon source
[Ref.: #43899]	ribose ChEBI	-	carbon source
[Ref.: #43899]	sodium acetate ChEBI	+	carbon source
[Ref.: #43899]	sodium citrate ChEBI	+	carbon source
[Ref.: #43899]	sodium pyruvate ChEBI	-	carbon source
[Ref.: #43899]	sodium succinate ChEBI	+	carbon source
[Ref.: #43899]	sorbitol ChEBI	+	carbon source
[Ref.: #43899]	sorbose ChEBI	-	carbon source
[Ref.: #43899]	starch ChEBI	-	degradation
[Ref.: #43899]	sucrose ChEBI	-	carbon source
[Ref.: #43899]	tween 20 ChEBI	-	degradation
[Ref.: #43899]	tween 40 ChEBI	-	degradation
[Ref.: #43899]	tween 60 ChEBI	-	degradation
[Ref.: #43899]	tween 80 ChEBI	-	degradation
[Ref.: #43899]	xylitol ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43899]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43899]	catalase	+	1.11.1.6
[Ref.: #43899]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43899]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43899]

Fatty acid	Percentage
C16:0	33.4
C16:1ω7c / C16:1ω6c	21.3
C18:1ω9c	13.4
10-methyl C18:0	10.9
C19:0	6.8
C20:0	3.3
C14:0	2.5
C17:1ω8c	2.1
C17:1 iso ω9c / C16:0 10-methyl	1.1
C17:0	1
C18:0	0.8
10-methyl C17:0	0.8
C19:1ω11c / C19:1ω9c	0.6
C12:0	0.5
C13:0	0.4
C20:4ω6,9,12,15c	0.3
C16:0 iso	0.2
C15:1ω8c	0.2
C15:1ω5c	0.2
C17:1ω5c	0.1
C15:0 anteiso	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42980]	Sample type/isolated from	soil
[Ref.: #42980]	Geographic location (country and/or sea, region)	Yunnan province, Tengchong, Heshun old town
[Ref.: #42980]	Country	China
[Ref.: #42980]	Country ISO 3 Code	CHN
[Ref.: #42980]	Continent	Asia

[Ref.: #43899]	Sample type/isolated from	soil sample from Heshun old town , Tengchong, Yunnan province, south-west PR China
[Ref.: #43899]	Geographic location (country and/or sea, region)	Heshun old town , Tengchong, Yunnan province
[Ref.: #43899]	Country	China
[Ref.: #43899]	Country ISO 3 Code	CHN
[Ref.: #43899]	Continent	Asia
[Ref.: #43899]	Enrichment culture	ISP 2 agar
[Ref.: #43899]	Enrichment culture composition	supplemented with nalidixic acid 25 mg/L, nystatin 50 mg/L
[Ref.: #43899]	Enrichment culture duration	2 weeks
[Ref.: #43899]	Enrichment culture temperature	28 ̊C

[Ref.: #67770]	Sample type/isolated from	Soil from Heshun old town in Tengchong
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yunnan Province, south-west China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42980]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42980]	Nocardia sp. strain CFH S0067 16S ribosomal RNA gene, partial sequence	KY039322	1541	GenBank ENA	1821 tax ID

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GC-content

66.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #42980]

Culture collection no.

DSM 46764, JCM 30085, CFH S0067

[Ref.: #96671]

SI-ID 400258

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardia yunnanensis sp. nov., an actinomycete isolated from a soil sample.	Zhang JX, Ming H, Zhao ZL, Ji WL, Chang XL, Zhang LY, Cheng LJ, Meng XL, Li WJ, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003600	2019	*
Phylogeny	Nocardia heshunensis sp. nov., an actinomycete isolated from soil.	Huang JR, Ming H, Duan YY, Li S, Zhang LY, Ji WL, Zhao ZL, Meng XL, Li WJ, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.002143	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42980

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46764

#43899

Jian-Rong Huang, Hong Ming, Yan-Yan Duan, Shuai Li, Ling-Yu Zhang, Wei-Li Ji, Zhuo-Li Zhao, Xiao-Lin Meng, Wen-Jun Li, Guo-Xing Nie: Nocardia heshunensis sp. nov., an actinomycete isolated from soil. IJSEM 67: 3467 - 3473 2017 ( DOI 10.1099/ijsem.0.002143 , PubMed 28875906 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#96671

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400258.1 )

* These data were automatically processed and therefore are not curated

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