Name: Roseobacter ponti MM-7
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140322

Type strain:

Species: Roseobacter ponti

Strain Designation: MM-7

Culture col. no.: DSM 106830, NBRC 112431

Strain history: <- NBRC; NBRC 112431 <- J. H. Yoon, Sungkyunkwan University, Suwon, Korea; MM-7

NCBI tax ID(s): 1891787 (species)

SI-ID 408175

DSM 106830

Special Collections

Section

Roseobacter ponti MM-7 is an aerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from Seawater.

coccus-shaped
colony-forming
Gram-negative
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Roseobacter*
	Species	**Roseobacter ponti**
	Full Scientific Name (LPSN)	Roseobacter ponti Jung et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43325]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.333

[Ref.: #43325]	Cell length	1.5-1.8 µm

[Ref.: #43325]	Cell width	1.0-1.2 µm

[Ref.: #43325]	Cell shape	coccus-shaped
[Ref.: #43325]	Cell shape	oval-shaped

[Ref.: #43325]	Motility	no

[Ref.: #43325]	Colony size	0.1-0.7 mm
[Ref.: #43325]	Colony color	cream
[Ref.: #43325]	Colony shape	circular
[Ref.: #43325]	Incubation period	5 days
[Ref.: #43325]	Cultivation medium used	Marine agar 2216

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43325]

Culture medium

Marine agar (MA)

[Ref.: #43325]

Culture medium growth

[Ref.: #42929]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #42929]

Culture medium growth

[Ref.: #42929]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42929]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #42929]		growth	25 °C
[Ref.: #43325]	no	growth	37 °C
[Ref.: #43325]		growth	4-35 °C
[Ref.: #43325]		optimum	25 °C

	Kind of pH		pH
[Ref.: #43325]		optimum	7-8
[Ref.: #43325]		growth	6
[Ref.: #43325]	no	growth	5.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43325]

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %90.704
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43325]		NaCl		optimum	2-3	%(w/v)
[Ref.: #43325]		NaCl		growth	1-5	%(w/v)

[Ref.: #43325]

Observation

ubiquinone Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43325]	benzoate ChEBI	-	carbon source
[Ref.: #43325]	benzoate ChEBI	-	energy source
[Ref.: #43325]	casamino acids	-	growth
[Ref.: #43325]	casein	-	hydrolysis
[Ref.: #43325]	casitone	+	growth
[Ref.: #43325]	cellobiose ChEBI	-	carbon source
[Ref.: #43325]	cellobiose ChEBI	-	energy source
[Ref.: #43325]	cellobiose ChEBI	-	builds acid from
[Ref.: #43325]	citrate ChEBI	-	carbon source
[Ref.: #43325]	citrate ChEBI	-	energy source
[Ref.: #43325]	D-fructose ChEBI	-	carbon source
[Ref.: #43325]	D-fructose ChEBI	-	energy source
[Ref.: #43325]	D-fructose ChEBI	-	builds acid from
[Ref.: #43325]	D-galactose ChEBI	-	carbon source
[Ref.: #43325]	D-galactose ChEBI	-	energy source
[Ref.: #43325]	D-galactose ChEBI	-	builds acid from
[Ref.: #43325]	D-glucose ChEBI	-	carbon source
[Ref.: #43325]	D-glucose ChEBI	-	energy source
[Ref.: #43325]	D-glucose ChEBI	-	builds acid from
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #43325]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43325]	D-mannose ChEBI	-	carbon source
[Ref.: #43325]	D-mannose ChEBI	-	energy source
[Ref.: #43325]	D-mannose ChEBI	-	builds acid from
[Ref.: #43325]	D-ribose ChEBI	-	builds acid from
[Ref.: #43325]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43325]	D-xylose ChEBI	-	carbon source
[Ref.: #43325]	D-xylose ChEBI	-	energy source
[Ref.: #43325]	D-xylose ChEBI	-	builds acid from
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43325]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #43325]	formate ChEBI	-	carbon source
[Ref.: #43325]	formate ChEBI	-	energy source
[Ref.: #43325]	gelatin ChEBI	-	hydrolysis
[Ref.: #43325]	hypoxanthine ChEBI	+	hydrolysis
[Ref.: #43325]	indole-3-acetic acid ChEBI	-	carbon source
[Ref.: #43325]	indole-3-acetic acid ChEBI	-	energy source
[Ref.: #43325]	L-arabinose ChEBI	-	carbon source
[Ref.: #43325]	L-arabinose ChEBI	-	energy source
[Ref.: #43325]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43325]	L-glutamate ChEBI	-	carbon source
[Ref.: #43325]	L-glutamate ChEBI	-	energy source
[Ref.: #43325]	L-malate ChEBI	-	carbon source
[Ref.: #43325]	L-malate ChEBI	-	energy source
[Ref.: #43325]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43325]	lactose ChEBI	-	builds acid from
[Ref.: #43325]	maltose ChEBI	-	carbon source
[Ref.: #43325]	maltose ChEBI	-	energy source
[Ref.: #43325]	maltose ChEBI	-	builds acid from
[Ref.: #43325]	melezitose ChEBI	-	builds acid from
[Ref.: #43325]	melibiose ChEBI	-	builds acid from
[Ref.: #43325]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43325]	nitrate ChEBI	-	reduction
[Ref.: #43325]	peptone	+/-	growth
[Ref.: #43325]	pyruvate ChEBI	-	carbon source
[Ref.: #43325]	pyruvate ChEBI	-	energy source
[Ref.: #43325]	raffinose ChEBI	-	builds acid from
[Ref.: #43325]	salicin ChEBI	-	carbon source
[Ref.: #43325]	salicin ChEBI	-	energy source
[Ref.: #43325]	starch ChEBI	-	hydrolysis
[Ref.: #43325]	succinate ChEBI	-	carbon source
[Ref.: #43325]	succinate ChEBI	-	energy source
[Ref.: #43325]	sucrose ChEBI	-	carbon source
[Ref.: #43325]	sucrose ChEBI	-	energy source
[Ref.: #43325]	sucrose ChEBI	-	builds acid from
[Ref.: #43325]	trehalose ChEBI	-	carbon source
[Ref.: #43325]	trehalose ChEBI	-	energy source
[Ref.: #43325]	trehalose ChEBI	-	builds acid from
[Ref.: #43325]	tryptone	+	growth
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #43325]	tween 20 ChEBI	+	hydrolysis
[Ref.: #43325]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43325]	tween 60 ChEBI	+	hydrolysis
[Ref.: #43325]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43325]	tyrosine ChEBI	+	hydrolysis
[Ref.: #43325]	urea ChEBI	-	hydrolysis
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43325]	xanthine ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43325]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43325]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #43325]	cefalotin ChEBI	yes	30	µg (disc)
[Ref.: #43325]	chloramphenicol ChEBI	yes	100	µg (disc)
[Ref.: #43325]	gentamicin ChEBI				yes	30	µg (disc)
[Ref.: #43325]	kanamycin ChEBI	yes	30
[Ref.: #43325]	lincomycin ChEBI				yes	15	µg (disc)
[Ref.: #43325]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43325]	novobiocin ChEBI	yes	5	µg (disc)
[Ref.: #43325]	oleandomycin ChEBI	yes	15	µg (disc)
[Ref.: #43325]	penicillin g ChEBI	yes	20	µg (disc)
[Ref.: #43325]	polymyxin b ChEBI	yes	100	Unit (disc)
[Ref.: #43325]	streptomycin ChEBI	yes	50	µg (disc)
[Ref.: #43325]	tetracycline ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #43325]	acid phosphatase	-	3.1.3.2
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #43325]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #43325]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #43325]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #43325]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #43325]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #43325]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #43325]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #43325]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #43325]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #43325]	catalase	+	1.11.1.6
[Ref.: #43325]	cystine arylamidase	-	3.4.11.3
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #43325]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43325]	esterase (C 4)	-
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #43325]	esterase Lipase (C 8)	-
[Ref.: #43325]	leucine arylamidase	+	3.4.11.1
[Ref.: #43325]	lipase (C 14)	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #43325]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #43325]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #43325]	trypsin	-	3.4.21.4
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #43325]	valine arylamidase	-
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43325]

Fatty acid	Percentage
C18:1ω6c / C18:1ω7c	78
11-Methyl C18:1ω7c	5.9
C16:0	4.2
C10:0 3OH	3.2
C13:0 iso 3OH	2
C15:1 iso F	1.7
C18:0	1.4
C17:0	1.1
anteiso-C15:0 A	0.8
C15:1 iso h / C13:0 3OH	0.8
C16:1ω6c / C16:1ω7c	0.8

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #42929]	24863	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.
[Ref.: #42929]	24690	+	-	-	-	-	+	+	-	+	+	+	+	+	+	+	-	+/-	+	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #42929]	14181	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #42929]	14037	-	+	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42929]	Sample type/isolated from	Seawater
[Ref.: #42929]	Geographic location (country and/or sea, region)	Mallipo,Taean, Yellow Sea
[Ref.: #42929]	Country	Republic of Korea
[Ref.: #42929]	Country ISO 3 Code	KOR
[Ref.: #42929]	Continent	Asia

[Ref.: #43325]	Sample type/isolated from	seawater of Mallipo, Taean, Korea
[Ref.: #43325]	Geographic location (country and/or sea, region)	Mallipo, Taean
[Ref.: #43325]	Country	Republic of Korea
[Ref.: #43325]	Country ISO 3 Code	KOR
[Ref.: #43325]	Continent	Asia
[Ref.: #43325]	Enrichment culture	Marine agar 2216
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX756455 (>99% sequence identity) for Sulfitobacter from Microbeatlas

Aquatic Samples	222
Soil Samples
Animal Samples	4
Plant Samples
Total Samples	226

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42929]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43325]	Roseobacter ponti strain MM-7 16S ribosomal RNA gene, partial sequence	KX756455		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Roseobacter ponti DSM 106830	GCA_012932215	complete	GenBank ENA	1891787 tax ID	*
[Ref.: #66792]	Roseobacter ponti strain DSM 106830	1891787.3	complete	PATRIC	1891787 tax ID	*

[Ref.: #43325]

GC-content

60.8 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Roseobacter ponti DSM 106830 NCBI: GCA_012932215.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Roseobacter ponti DSM 106830 NCBI: GCA_012932215.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99.333	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.509	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	82.59	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	90.704	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.475	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	63.463	no

Availability in culture collections External links

[Ref.: #42929]

Culture collection no.

DSM 106830, NBRC 112431

[Ref.: #96626]

SI-ID 408175

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseobacter cerasinus sp. nov., isolated from a fish farm.	Muramatsu S, Kanamuro M, Sato-Takabe Y, Hirose S, Muramatsu Y, Takaichi S, Hanada S	Int J Syst Evol Microbiol	10.1099/ijsem.0.004360	2020	*
Phylogeny	Roseobacter ponti sp. nov., isolated from seawater.	Jung YT, Park S, Lee JS, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijsem.0.001922	2017	*
Genetics	Complete Genome of Roseobacter ponti DSM 106830T.	Hollensteiner J, Schneider D, Poehlein A, Daniel R	Genome Biol Evol	10.1093/gbe/evaa114	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42929

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106830

#43325

Yong-Taek Jung, Sooyeon Park, Jung-Sook Lee, Jung-Hoon Yoon: Roseobacter ponti sp. nov., isolated from seawater. IJSEM 67: 2189 - 2194 2017 ( DOI 10.1099/ijsem.0.001922 , PubMed 28671525 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96626

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408175.1 )

* These data were automatically processed and therefore are not curated

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Roseobacter ponti DSM 106830

Roseobacter ponti DSM 106830

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