Name: Cysteiniphilum litorale DSM 101832
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140321

Type strain:

Species: Cysteiniphilum litorale

Culture col. no.: DSM 101832, CGMCC 1.15758, KCTC 52386, NBRC 112441, SYSU D3-2

Strain history: <- Wenjun Li, School of Life Sciences, Sun Yat-Sen Univ., China

NCBI tax ID(s): 2056700 (species)

SI-ID 400333

DSM 101832

Special Collections

Section

Cysteiniphilum litorale DSM 101832 is a microaerophile, Gram-negative, coccus-shaped bacterium that was isolated from seawater.

coccus-shaped
Gram-negative
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Beggiatoales*
	Family	*Francisellaceae*
	Genus	*Cysteiniphilum*
	Species	**Cysteiniphilum litorale**
	Full Scientific Name (LPSN)	Cysteiniphilum litorale Liu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43323]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98

[Ref.: #43323]	Cell length	0.6-0.9 µm

[Ref.: #43323]	Cell width	0.4-0.5 µm

[Ref.: #43323]	Cell shape	coccus-shaped

[Ref.: #43323]	Motility	no
[Ref.: #67771]	Motility	no

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BCYE-alpha agar

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Culture medium growth

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Culture medium

Cystein heart agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Haemophilus chocolate 2 agar

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Culture medium growth

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Culture medium

HTM agar

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Culture medium growth

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Culture medium

Columbia blood agar

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Culture medium growth

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Culture medium

COLUMBIA-GLUCOSE-CYSTEINE-AGAR (DSMZ Medium 429c)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Kligler's iron agar

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

		Temperature
[Ref.: #42928]	growth	37 °C
[Ref.: #43323]	optimum	28-32 °C
[Ref.: #43323]	growth	18-37 °C
[Ref.: #67771]	growth	37 °C

	pH	Kind of pH		pH
[Ref.: #43323]			optimum	7-8
[Ref.: #43323]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43323]	Oxygen tolerance	microaerophile
[Ref.: #67771]	Oxygen tolerance	microaerophile

[Ref.: #43323]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %94.26
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43323]		NaCl		growth	0-7.5	%(w/v)
[Ref.: #43323]		NaCl	no	growth	1	%

[Ref.: #43323]	Observation	ubiquinone-8
[Ref.: #67771]	Observation	quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43323]	acetate ChEBI	+	carbon source
[Ref.: #43323]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #43323]	D-fructose ChEBI	+	builds acid from
[Ref.: #43323]	D-fructose ChEBI	+	carbon source
[Ref.: #43323]	D-glucose ChEBI	+	builds acid from
[Ref.: #43323]	D-serine ChEBI	+	carbon source
[Ref.: #43323]	esculin ChEBI	-	hydrolysis
[Ref.: #43323]	glycerol ChEBI	+	carbon source
[Ref.: #43323]	glycine-proline ChEBI	+	carbon source
[Ref.: #43323]	L-alanine ChEBI	+	carbon source
[Ref.: #43323]	L-aspartate ChEBI	+	carbon source
[Ref.: #43323]	L-glutamate ChEBI	+	carbon source
[Ref.: #43323]	L-histidine ChEBI	+	carbon source
[Ref.: #43323]	L-serine ChEBI	+	carbon source
[Ref.: #43323]	maltose ChEBI	-	builds acid from
[Ref.: #43323]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #43323]	nitrate ChEBI	-	reduction
[Ref.: #43323]	sucrose ChEBI	-	builds acid from
[Ref.: #43323]	tryptophan ChEBI	-	energy source
[Ref.: #43323]	tween 40 ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #43323]	1 % sodium lactate	yes
[Ref.: #43323]	cephalosporin ChEBI		yes
[Ref.: #43323]	chloramphenicol ChEBI	yes
[Ref.: #43323]	ciprofloxacin ChEBI	yes
[Ref.: #43323]	doxycycline ChEBI	yes
[Ref.: #43323]	gentamicin ChEBI	yes
[Ref.: #43323]	levofloxacin ChEBI	yes
[Ref.: #43323]	lincomycin ChEBI	yes
[Ref.: #43323]	rifamycin sv ChEBI	yes
[Ref.: #43323]	tetracycline ChEBI	yes

	Metabolite production	Metabolite	Production
[Ref.: #43323]		acetoin ChEBI	no
[Ref.: #43323]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #43323]		acetoin ChEBI	-
[Ref.: #43323]		indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #43323]	acid phosphatase	+	3.1.3.2
[Ref.: #43323]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43323]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43323]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43323]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43323]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43323]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43323]	beta-galactosidase	-	3.2.1.23
[Ref.: #43323]	beta-glucosidase	-	3.2.1.21
[Ref.: #43323]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43323]	beta-lactamase	+	3.5.2.6
[Ref.: #43323]	catalase	+	1.11.1.6
[Ref.: #43323]	cystine arylamidase	-	3.4.11.3
[Ref.: #43323]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43323]	esterase (C 4)	+
[Ref.: #43323]	esterase Lipase (C 8)	+
[Ref.: #43323]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #43323]	leucine arylamidase	-	3.4.11.1
[Ref.: #43323]	lipase (C 14)	-
[Ref.: #43323]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43323]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43323]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43323]	proline-arylamidase	-	3.4.11.5
[Ref.: #43323]	trypsin	+	3.4.21.4
[Ref.: #43323]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43323]	urease	-	3.5.1.5
[Ref.: #43323]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C15:0 anteiso	34.4
C18:0	20.4
C17:0 anteiso	10.7
C16:0 iso	5.9
C14:0 iso	3.4
C18:1ω9c	3.2
C14:0 3OH / C16:1 iso I	2.1
C18:0 iso	1.9
C12:0 3OH	1.3
C19:0 anteiso	1.1
C16:0 3OH	1
C14:0	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42928]	Sample type/isolated from	seawater
[Ref.: #42928]	Geographic location (country and/or sea, region)	seashore of Wailingding island (113.27° E 23.12° N) near the estuary of Pearl River
[Ref.: #42928]	Country	China
[Ref.: #42928]	Country ISO 3 Code	CHN
[Ref.: #42928]	Continent	Asia
[Ref.: #42928]	Geographic location	23.1200°/113.2700°

[Ref.: #43323]	Sample type/isolated from	Seawater samples from the seashore of Wailingding island, near the estuary of Pearl River in southern China
[Ref.: #43323]	Sampling date	2014-09
[Ref.: #43323]	Geographic location (country and/or sea, region)	Wailingding island
[Ref.: #43323]	Country	China
[Ref.: #43323]	Country ISO 3 Code	CHN
[Ref.: #43323]	Continent	Asia
[Ref.: #43323]	Geographic location	23.0000°/113.0000°
[Ref.: #43323]	Enrichment culture	BCYEa supplemented with 3 g/L glycine, 1 mg/L vancomycin, 80 000 IU/L polymyxin B sulphate, 80 mg/L cycloheximide
[Ref.: #43323]	Enrichment culture duration	7 days

[Ref.: #67771]	Sample type/isolated from	From seawater
[Ref.: #67771]	Geographic location (country and/or sea, region)	the seashore of Wailingding island, near the estuary of Pearl River, Guangdong province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42928]	Cysteiniphilum litorale strain SYSU D3-2 16S ribosomal RNA gene, partial sequence	KX817994	1533	GenBank ENA	2056700 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cysteiniphilum litorale DSM 101832	GCA_003428165	contig	GenBank ENA	2056700 tax ID	*
[Ref.: #66792]	Cysteiniphilum litorale CGMCC 1.15758	GCA_014643575	scaffold	GenBank ENA	2056700 tax ID	*
[Ref.: #66792]	Cysteiniphilum litorale strain CGMCC 1.15758	2056700.4	wgs	PATRIC	2056700 tax ID	*
[Ref.: #66792]	Cysteiniphilum litorale strain DSM 101832	2056700.3	wgs	PATRIC	2056700 tax ID	*
[Ref.: #66792]	Cysteiniphilum litorale DSM 101832	2831476743	draft	JGI IMG/M	2056700 tax ID	*

[Ref.: #42928]	GC-content	38.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	38.1 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cysteiniphilum litorale CGMCC 1.15758 NCBI: GCA_014643575.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cysteiniphilum litorale CGMCC 1.15758 NCBI: GCA_014643575.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.286	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	51.537	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	94.26	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.069	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	82.87	yes

Availability in culture collections External links

[Ref.: #42928]

Culture collection no.

DSM 101832, CGMCC 1.15758, KCTC 52386, NBRC 112441, SYSU D3-2

[Ref.: #96625]

SI-ID 400333

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Fastidiosibacter lacustris gen. nov., sp. nov., isolated from a lake water sample, and proposal of Fastidiosibacteraceae fam. nov. within the order Thiotrichales.	Xiao M, Salam N, Liu L, Jiao JY, Zheng ML, Wang J, Li S, Chen C, Li WJ, Qu PH	Int J Syst Evol Microbiol	10.1099/ijsem.0.002510	2017	*
Phylogeny	Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater.	Liu L, Salam N, Jiao JY, E SM, Chen C, Fang BZ, Xiao M, Li M, Li WJ, Qu PH	Int J Syst Evol Microbiol	10.1099/ijsem.0.001917	2017	*
Phylogeny	Cysteiniphilum marinum sp. nov., isolated from coastal seawater.	Luo HM, Feng JH, Li LH, Li MM, Liu RQ, Chen F, Lin Y, Li WJ, Qu PH, Xiao M	Antonie Van Leeuwenhoek	10.1007/s10482-021-01579-7	2021	*
Phylogeny	Facilibium subflavum gen. nov., sp. nov. and Cysteiniphilum halobium sp. nov., new members of the family Fastidiosibacteraceae isolated from coastal seawater.	Xiao M, Zheng ML, Salam N, Jiao JY, Dong L, Liu L, Chen C, Li WJ, Qu PH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003676	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42928

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101832

#43323

Lan Liu, Nimaichand Salam, Jian-Yu Jiao, Shun-Mei E, Cha Chen, Bao-Zhu Fang, Min Xiao, Mo Li, Wen-Jun Li, Ping-Hua Qu: Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater. IJSEM 67: 2178 - 2183 2017 ( DOI 10.1099/ijsem.0.001917 , PubMed 28671520 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96625

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400333.1 )

* These data were automatically processed and therefore are not curated

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Cysteiniphilum litorale CGMCC 1.15758

Cysteiniphilum litorale CGMCC 1.15758

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