Name: Pseudomonas lactis WS 4992
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140299

Type strain:

Species: Pseudomonas lactis

Strain Designation: WS 4992

Culture col. no.: DSM 29167, LMG 28435

Strain history: <- M. Wenning, (ZIEL) TU Munich, Microbiology, Freising-Weihenstephan, Germany; WS 4992 <- M. Freiherr von Neubeck

NCBI tax ID(s): 1615674 (species)

SI-ID 403013

DSM 29167

Special Collections

Other strains from Pseudomonas lactis

Section

Pseudomonas lactis WS 4992 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from bovine raw milk.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas lactis**
	Full Scientific Name (LPSN)	Pseudomonas lactis von Neubeck et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43273]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99

[Ref.: #43273]	Cell length	2.6 µm

[Ref.: #43273]	Cell width	0.75 µm

[Ref.: #43273]	Cell shape	rod-shaped

[Ref.: #43273]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %90.741

[Ref.: #43273]	Colony size	0.5-1.0 mm
[Ref.: #43273]	Colony color	beige
[Ref.: #43273]	Colony shape	circular
[Ref.: #43273]	Incubation period	1 day
[Ref.: #43273]	Cultivation medium used	TSG

[Ref.: #43273]	Pigment production	yes
[Ref.: #43273]	Pigment name	Fluorescent pigments

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TSG

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

cetrimide agar

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Culture medium growth

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #42906]	growth	28 °C
[Ref.: #43273]	growth	27-31 °C
[Ref.: #43273]	growth	4-35 °C

	pH	Kind of pH		pH
[Ref.: #43273]			optimum	6.0-7.0
[Ref.: #43273]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43273]	Oxygen tolerance	aerobe
[Ref.: #69480]	Oxygen tolerance	aerobe Confidence in %91.701

[Ref.: #43273]	Type of spore	endospore
[Ref.: #43273]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43273]		NaCl		growth	0-6.0	%(w/v)
[Ref.: #43273]		NaCl		optimum	0-1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43273]	amygdalin ChEBI	-	carbon source
[Ref.: #43273]	arbutin ChEBI	-	carbon source
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #43273]	cellobiose ChEBI	-	carbon source
[Ref.: #43273]	cellobiose ChEBI	+	builds acid from
[Ref.: #43273]	D-arabinose ChEBI	-	carbon source
[Ref.: #43273]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43273]	D-arabitol ChEBI	+	carbon source
[Ref.: #43273]	D-fructose ChEBI	+	carbon source
[Ref.: #43273]	D-fructose ChEBI	+	builds acid from
[Ref.: #43273]	D-fucose ChEBI	-	carbon source
[Ref.: #43273]	D-fucose ChEBI	+	builds acid from
[Ref.: #43273]	D-galactose ChEBI	+	carbon source
[Ref.: #43273]	D-galactose ChEBI	+	builds acid from
[Ref.: #43273]	D-glucose ChEBI	+	carbon source
[Ref.: #43273]	D-glucose ChEBI	+	builds acid from
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #43273]	D-lyxose ChEBI	+	carbon source
[Ref.: #43273]	D-lyxose ChEBI	+	builds acid from
[Ref.: #43273]	D-mannitol ChEBI	+	carbon source
[Ref.: #43273]	D-mannitol ChEBI	+	builds acid from
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	D-mannose ChEBI	+	carbon source
[Ref.: #43273]	D-mannose ChEBI	+	builds acid from
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	D-ribose ChEBI	+	carbon source
[Ref.: #43273]	D-ribose ChEBI	+	builds acid from
[Ref.: #43273]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43273]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #43273]	D-tagatose ChEBI	-	carbon source
[Ref.: #43273]	D-xylose ChEBI	+	carbon source
[Ref.: #43273]	D-xylose ChEBI	+	builds acid from
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	erythritol ChEBI	+	carbon source
[Ref.: #43273]	erythritol ChEBI	+	builds acid from
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43273]	galactitol ChEBI	-	carbon source
[Ref.: #43273]	gelatin ChEBI	+	hydrolysis
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #43273]	gentiobiose ChEBI	-	carbon source
[Ref.: #43273]	gentiobiose ChEBI	+	builds acid from
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	glycerol ChEBI	+	carbon source
[Ref.: #43273]	glycerol ChEBI	+	builds acid from
[Ref.: #43273]	glycogen ChEBI	-	carbon source
[Ref.: #43273]	L-arabinose ChEBI	+	carbon source
[Ref.: #43273]	L-arabinose ChEBI	+	builds acid from
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	L-arabitol ChEBI	+	carbon source
[Ref.: #43273]	L-arabitol ChEBI	+	builds acid from
[Ref.: #43273]	L-fucose ChEBI	-	carbon source
[Ref.: #43273]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43273]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43273]	L-sorbose ChEBI	-	carbon source
[Ref.: #43273]	L-xylose ChEBI	-	carbon source
[Ref.: #43273]	lactose ChEBI	-	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	maltose ChEBI	-	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #43273]	melezitose ChEBI	-	carbon source
[Ref.: #43273]	melibiose ChEBI	-	carbon source
[Ref.: #43273]	melibiose ChEBI	+	builds acid from
[Ref.: #43273]	methyl alpha-D-glucopyranoside ChEBI	-	carbon source
[Ref.: #43273]	methyl alpha-D-mannoside ChEBI	-	carbon source
[Ref.: #43273]	methyl beta-D-xylopyranoside ChEBI	-	carbon source
[Ref.: #43273]	myo-inositol ChEBI	+	carbon source
[Ref.: #43273]	myo-inositol ChEBI	+	builds acid from
[Ref.: #43273]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #43273]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #43273]	nitrite ChEBI	-	reduction
[Ref.: #43273]	potassium gluconate ChEBI	+	carbon source
[Ref.: #43273]	raffinose ChEBI	-	carbon source
[Ref.: #43273]	ribitol ChEBI	+	carbon source
[Ref.: #43273]	ribitol ChEBI	+	builds acid from
[Ref.: #43273]	salicin ChEBI	-	carbon source
[Ref.: #43273]	skimmed milk	+	degradation
[Ref.: #43273]	starch ChEBI	-	hydrolysis
[Ref.: #43273]	sucrose ChEBI	+	carbon source
[Ref.: #43273]	sucrose ChEBI	+	builds acid from
[Ref.: #43273]	trehalose ChEBI	+	carbon source
[Ref.: #43273]	trehalose ChEBI	+	builds acid from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #43273]	turanose ChEBI	-	carbon source
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43273]	xylitol ChEBI	+	carbon source
[Ref.: #43273]	xylitol ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43273]	acetoin ChEBI	no
[Ref.: #43273]	hydrogen sulfide ChEBI	no
[Ref.: #43273]	indole ChEBI	no
[Ref.: #43273]	pyocyanine ChEBI	no
[Ref.: #68369]	indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #43273]		acetoin ChEBI	-
[Ref.: #68369]		indole ChEBI		-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #43273]	arginine dihydrolase	+	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #43273]	beta-galactosidase	-	3.2.1.23
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #43273]	catalase	+	1.11.1.6
[Ref.: #43273]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #43273]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43273]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43273]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43273]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43273]

Fatty acid	Percentage
C16:0	31.7
C16:1ω7c / C15:0 iso 2OH	29.5
C18:1ω7c	17
cyclo-C17:0	5.9
C12:0 2OH	4.1
C12:0 3OH	3.9
C10:0 3OH	2.8
C12:0	2.6
C18:0	1.1

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #42906]	24784	-	-	-	+	-	-	+	-	+	+	+	+	+	-	+	+	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42906]	Sample type/isolated from	bovine raw milk
[Ref.: #42906]	Geographic location (country and/or sea, region)	Southern Gemany
[Ref.: #42906]	Country	Germany
[Ref.: #42906]	Country ISO 3 Code	DEU
[Ref.: #42906]	Continent	Europe

[Ref.: #43273]	Sample type/isolated from	raw bovine milk
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP756923 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	1631
Soil Samples	874
Animal Samples	3216
Plant Samples	2451
Total Samples	8172

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42906]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42906]	Pseudomonas lactis strain DSM 29167 16S ribosomal RNA gene, partial sequence	KP756923	1428	GenBank ENA	1615674 tax ID
[Ref.: #124043]	Pseudomonas lactis strain DSM 29167 16S ribosomal RNA gene, partial sequence.	OR660289	1053	GenBank ENA	1615674 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas lactis DSM 29167	GCA_001439845	contig	GenBank ENA	1615674 tax ID	*
[Ref.: #66792]	Pseudomonas sp. DSM 29167	1615674.3	wgs	PATRIC	1615674 tax ID	*
[Ref.: #66792]	Pseudomonas lactis DSM 29167	2660238313	draft	JGI IMG/M	1615674 tax ID	*

[Ref.: #43273]	GC-content	60 mol%
[Ref.: #42906]	GC-content	60.0 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas sp. DSM 29167 PATRIC: 1615674.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	98.941	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	84.228	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	91.701	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.997	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	90.741	no

Availability in culture collections External links

[Ref.: #42906]

Culture collection no.

DSM 29167, LMG 28435

[Ref.: #96604]

SI-ID 403013

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas lactucae sp. nov., a pathogen causing bacterial rot of lettuce in Japan.	Sawada H, Fujikawa T, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004917	2021	*
Phylogeny	Pseudomonas carnis sp. nov., isolated from meat.	Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Bantleon A, Goesmann A, Kalinowski J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003928	2020	*
Phylogeny	Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk.	von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001836	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42906

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29167

#43273

Mario von Neubeck, Christopher Huptas, Claudia Glück, Manuel Krewinkel, Marina Stoeckel, Timo Stressler, Lutz Fischer, Jörg Hinrichs, Siegfried Scherer and Mareike Wenning: Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp.nov., isolated from bovine raw milk. IJSEM 67: 1656 - 1664 2017 ( DOI 10.1099/ijsem.0.001836 , PubMed 28141500 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96604

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403013.1 )

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Pseudomonas sp. DSM 29167

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