Name: Pseudomonas paralactis WS 4672
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140300

Type strain:

Species: Pseudomonas paralactis

Strain Designation: WS 4672

Culture col. no.: DSM 29164, LMG 28439

Strain history: <- M. Wenning, (ZIEL), TU Munich, Microbiology, Germany; WS 4672 <- M. Freiherr von Neubeck

NCBI tax ID(s): 1615673 (species)

SI-ID 401223

DSM 29164

Section

Pseudomonas paralactis WS 4672 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from bovine raw milk.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas paralactis**
	Full Scientific Name (LPSN)	Pseudomonas paralactis von Neubeck et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43273]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #43273]	Cell length	1.5-5.5 µm

[Ref.: #43273]	Cell width	0.75 µm

[Ref.: #43273]	Cell shape	rod-shaped

[Ref.: #43273]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.777

[Ref.: #43273]	Colony size	0.5-1.0 mm
[Ref.: #43273]	Colony color	beige
[Ref.: #43273]	Colony shape	circular
[Ref.: #43273]	Incubation period	1 day
[Ref.: #43273]	Cultivation medium used	TSG

[Ref.: #43273]	Pigment production	yes
[Ref.: #43273]	Pigment name	Fluorescent pigments

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TSG

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

cetrimide agar

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Culture medium growth

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #42907]	growth	28 °C
[Ref.: #43273]	optimum	27-31 °C
[Ref.: #43273]	growth	4-35 °C

[Ref.: #42907]	Temperature range	mesophilic
[Ref.: #43273]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43273]			optimum	6.0-7.5
[Ref.: #43273]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43273]	Type of spore	endospore
[Ref.: #43273]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.986

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43273]		NaCl		growth	0-6.0	%(w/v)
[Ref.: #43273]		NaCl		optimum	0-1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43273]	amygdalin ChEBI	-	carbon source
[Ref.: #43273]	arbutin ChEBI	-	carbon source
[Ref.: #43273]	cellobiose ChEBI	-	carbon source
[Ref.: #43273]	cellobiose ChEBI	+	builds acid from
[Ref.: #43273]	D-arabinose ChEBI	-	carbon source
[Ref.: #43273]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43273]	D-arabitol ChEBI	+	carbon source
[Ref.: #43273]	D-fructose ChEBI	+	carbon source
[Ref.: #43273]	D-fructose ChEBI	+	builds acid from
[Ref.: #43273]	D-fucose ChEBI	-	carbon source
[Ref.: #43273]	D-fucose ChEBI	+	builds acid from
[Ref.: #43273]	D-galactose ChEBI	+	carbon source
[Ref.: #43273]	D-galactose ChEBI	+	builds acid from
[Ref.: #43273]	D-glucose ChEBI	+	carbon source
[Ref.: #43273]	D-glucose ChEBI	+	builds acid from
[Ref.: #43273]	D-lyxose ChEBI	+	carbon source
[Ref.: #43273]	D-lyxose ChEBI	+	builds acid from
[Ref.: #43273]	D-mannitol ChEBI	+	carbon source
[Ref.: #43273]	D-mannitol ChEBI	+	builds acid from
[Ref.: #43273]	D-mannose ChEBI	+	carbon source
[Ref.: #43273]	D-mannose ChEBI	+	builds acid from
[Ref.: #43273]	D-ribose ChEBI	+	carbon source
[Ref.: #43273]	D-ribose ChEBI	+	builds acid from
[Ref.: #43273]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43273]	D-tagatose ChEBI	-	carbon source
[Ref.: #43273]	D-xylose ChEBI	+	carbon source
[Ref.: #43273]	D-xylose ChEBI	+	builds acid from
[Ref.: #43273]	erythritol ChEBI	+	carbon source
[Ref.: #43273]	erythritol ChEBI	+	builds acid from
[Ref.: #43273]	galactitol ChEBI	-	carbon source
[Ref.: #43273]	gelatin ChEBI	+	hydrolysis
[Ref.: #43273]	gentiobiose ChEBI	-	carbon source
[Ref.: #43273]	gentiobiose ChEBI	+	builds acid from
[Ref.: #43273]	glycerol ChEBI	+	carbon source
[Ref.: #43273]	glycerol ChEBI	+	builds acid from
[Ref.: #43273]	glycogen ChEBI	-	carbon source
[Ref.: #43273]	L-arabinose ChEBI	+	carbon source
[Ref.: #43273]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43273]	L-arabitol ChEBI	+	carbon source
[Ref.: #43273]	L-arabitol ChEBI	+	builds acid from
[Ref.: #43273]	L-fucose ChEBI	-	carbon source
[Ref.: #43273]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43273]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43273]	L-sorbose ChEBI	-	carbon source
[Ref.: #43273]	L-xylose ChEBI	-	carbon source
[Ref.: #43273]	lactose ChEBI	-	carbon source
[Ref.: #43273]	maltose ChEBI	-	carbon source
[Ref.: #43273]	melezitose ChEBI	-	carbon source
[Ref.: #43273]	melibiose ChEBI	-	carbon source
[Ref.: #43273]	melibiose ChEBI	+	builds acid from
[Ref.: #43273]	methyl alpha-D-glucopyranoside ChEBI	-	carbon source
[Ref.: #43273]	methyl alpha-D-mannoside ChEBI	-	carbon source
[Ref.: #43273]	methyl beta-D-xylopyranoside ChEBI	-	carbon source
[Ref.: #43273]	myo-inositol ChEBI	+	carbon source
[Ref.: #43273]	myo-inositol ChEBI	+	builds acid from
[Ref.: #43273]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #43273]	nitrate ChEBI	-	reduction
[Ref.: #43273]	nitrite ChEBI	-	reduction
[Ref.: #43273]	potassium gluconate ChEBI	+	carbon source
[Ref.: #43273]	raffinose ChEBI	-	carbon source
[Ref.: #43273]	ribitol ChEBI	+	carbon source
[Ref.: #43273]	ribitol ChEBI	+	builds acid from
[Ref.: #43273]	salicin ChEBI	-	carbon source
[Ref.: #43273]	skimmed milk	+	degradation
[Ref.: #43273]	starch ChEBI	-	hydrolysis
[Ref.: #43273]	sucrose ChEBI	-	carbon source
[Ref.: #43273]	trehalose ChEBI	+	carbon source
[Ref.: #43273]	trehalose ChEBI	+	builds acid from
[Ref.: #43273]	turanose ChEBI	-	carbon source
[Ref.: #43273]	xylitol ChEBI	+	carbon source
[Ref.: #43273]	xylitol ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43273]	acetoin ChEBI	no
[Ref.: #43273]	hydrogen sulfide ChEBI	no
[Ref.: #43273]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43273]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #43273]	arginine dihydrolase	+	3.5.3.6
[Ref.: #43273]	beta-galactosidase	-	3.2.1.23
[Ref.: #43273]	catalase	+	1.11.1.6
[Ref.: #43273]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43273]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43273]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43273]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43273]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43273]

Fatty acid	Percentage
C16:1ω7c / C15:0 iso 2OH	33.1
C16:0	29.1
C18:1ω7c	18.4
C12:0	3.9
C12:0 3OH	3.8
C10:0 3OH	3.4
C12:0 2OH	3.4
cyclo-C17:0	3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42907]	Sample type/isolated from	bovine raw milk
[Ref.: #42907]	Country	Germany
[Ref.: #42907]	Country ISO 3 Code	DEU
[Ref.: #42907]	Continent	Europe

[Ref.: #43273]	Sample type/isolated from	raw bovine milk
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

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Global distribution of 16S sequence KP756921 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	1631
Soil Samples	874
Animal Samples	3216
Plant Samples	2451
Total Samples	8172

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42907]	Pseudomonas paralactis strain DSM 29164 16S ribosomal RNA gene, partial sequence	KP756921	1428	ENA	1615673 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas paralactis DSM 29164	GCA_001439735	contig	GenBank	1615673 tax ID	*
[Ref.: #66792]	Pseudomonas sp. DSM 29164	1615673.3	wgs	PATRIC	1615673 tax ID	*
[Ref.: #66792]	Pseudomonas paralactis DSM 29164	2693429756	draft	JGI IMG/M	1615673 tax ID	*

[Ref.: #43273]	GC-content	59.7 mol%
[Ref.: #42907]	GC-content	59.7 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas sp. DSM 29164 PATRIC: 1615673.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas sp. DSM 29164 PATRIC: 1615673.3
[Ref.: #69480]	motile	yes	90.844	yes
[Ref.: #69480]	gram-positive	no	98.489	no
[Ref.: #69480]	anaerobic	no	98.201	no
[Ref.: #69480]	aerobic	yes	95.218	yes
[Ref.: #69480]	halophile	no	91.573	yes
[Ref.: #69480]	spore-forming	no	95.252	no
[Ref.: #69480]	thermophile	no	99.777	yes
[Ref.: #69480]	glucose-util	yes	94.126	yes
[Ref.: #69480]	flagellated	yes	85.311	no
[Ref.: #69480]	glucose-ferment	no	90.225	no

Availability in culture collections External links

[Ref.: #42907]

Culture collection no.

DSM 29164, LMG 28439

[Ref.: #96605]

SI-ID 401223

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas carnis sp. nov., isolated from meat.	Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Bantleon A, Goesmann A, Kalinowski J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003928	2020	*
Phylogeny	Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk.	von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001836	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42907

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29164

#43273

Mario von Neubeck, Christopher Huptas, Claudia Glück, Manuel Krewinkel, Marina Stoeckel, Timo Stressler, Lutz Fischer, Jörg Hinrichs, Siegfried Scherer and Mareike Wenning: Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp.nov., isolated from bovine raw milk. IJSEM 67: 1656 - 1664 2017 ( DOI 10.1099/ijsem.0.001836 , PubMed 28141500 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96605

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401223.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas sp. DSM 29164

Pseudomonas sp. DSM 29164

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