Strain identifier
BacDive ID: 140299
Type strain:
Species: Pseudomonas lactis
Strain Designation: WS 4992
Strain history: <- M. Wenning, (ZIEL) TU Munich, Microbiology, Freising-Weihenstephan, Germany; WS 4992 <- M. Freiherr von Neubeck
NCBI tax ID(s): 1615674 (species)
General
@ref: 42906
BacDive-ID: 140299
DSM-Number: 29167
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming
description: Pseudomonas lactis WS 4992 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from bovine raw milk.
NCBI tax id
- NCBI tax id: 1615674
- Matching level: species
strain history
- @ref: 42906
- history: <- M. Wenning, (ZIEL) TU Munich, Microbiology, Freising-Weihenstephan, Germany; WS 4992 <- M. Freiherr von Neubeck
doi: 10.13145/bacdive140299.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas lactis
- full scientific name: Pseudomonas lactis von Neubeck et al. 2017
@ref: 42906
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Burkholderiales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas lactis
full scientific name: Pseudomonas lactis von Neubeck et al. 2017
strain designation: WS 4992
type strain: yes
Morphology
cell morphology
- @ref: 43273
- gram stain: negative
- cell length: 2.6 µm
- cell width: 0.75 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 43273
- colony size: 0.5-1.0 mm
- colony color: beige
- colony shape: circular
- incubation period: 1 day
- medium used: TSG
pigmentation
- @ref: 43273
- production: yes
- name: Fluorescent pigments
multimedia
- @ref: 42906
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_29167.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
43273 | TSG | yes | trypticase soy agar (TSA; Roth) supplemented with 1 % glucose | |
43273 | Reasoner's 2A agar (R2A) | yes | ||
43273 | cetrimide agar | yes | ||
42906 | NUTRIENT AGAR (DSMZ Medium 1) | yes | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | https://mediadive.dsmz.de/medium/1 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43273 | positive | growth | 27-31 | mesophilic |
43273 | positive | growth | 4-35 | |
42906 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
43273 | positive | growth | 5-8 |
43273 | positive | optimum | 6.0-7.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 43273
- oxygen tolerance: aerobe
spore formation
- @ref: 43273
- type of spore: endospore
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43273 | NaCl | positive | growth | 0-6.0 %(w/v) |
43273 | NaCl | positive | optimum | 0-1 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43273 | 15963 | ribitol | + | carbon source |
43273 | 18333 | D-arabitol | + | carbon source |
43273 | 15824 | D-fructose | + | carbon source |
43273 | 17634 | D-glucose | + | carbon source |
43273 | 12936 | D-galactose | + | carbon source |
43273 | 62318 | D-lyxose | + | carbon source |
43273 | 16899 | D-mannitol | + | carbon source |
43273 | 16024 | D-mannose | + | carbon source |
43273 | 16988 | D-ribose | + | carbon source |
43273 | 17924 | D-sorbitol | + | carbon source |
43273 | 17992 | sucrose | + | carbon source |
43273 | 27082 | trehalose | + | carbon source |
43273 | 65327 | D-xylose | + | carbon source |
43273 | 17113 | erythritol | + | carbon source |
43273 | 17754 | glycerol | + | carbon source |
43273 | 17268 | myo-inositol | + | carbon source |
43273 | 30849 | L-arabinose | + | carbon source |
43273 | 18403 | L-arabitol | + | carbon source |
43273 | 506227 | N-acetylglucosamine | + | carbon source |
43273 | 32032 | potassium gluconate | + | carbon source |
43273 | 17151 | xylitol | + | carbon source |
43273 | 27613 | amygdalin | - | carbon source |
43273 | 18305 | arbutin | - | carbon source |
43273 | 17108 | D-arabinose | - | carbon source |
43273 | 17057 | cellobiose | - | carbon source |
43273 | 28847 | D-fucose | - | carbon source |
43273 | 17716 | lactose | - | carbon source |
43273 | 17306 | maltose | - | carbon source |
43273 | 6731 | melezitose | - | carbon source |
43273 | 28053 | melibiose | - | carbon source |
43273 | 16634 | raffinose | - | carbon source |
43273 | 16443 | D-tagatose | - | carbon source |
43273 | 32528 | turanose | - | carbon source |
43273 | 16813 | galactitol | - | carbon source |
43273 | 28066 | gentiobiose | - | carbon source |
43273 | 28087 | glycogen | - | carbon source |
43273 | 18287 | L-fucose | - | carbon source |
43273 | 62345 | L-rhamnose | - | carbon source |
43273 | 17266 | L-sorbose | - | carbon source |
43273 | 65328 | L-xylose | - | carbon source |
43273 | 320061 | methyl alpha-D-glucopyranoside | - | carbon source |
43273 | 43943 | methyl alpha-D-mannoside | - | carbon source |
43273 | 74863 | methyl beta-D-xylopyranoside | - | carbon source |
43273 | 17814 | salicin | - | carbon source |
43273 | 17754 | glycerol | + | builds acid from |
43273 | 17113 | erythritol | + | builds acid from |
43273 | 30849 | L-arabinose | + | builds acid from |
43273 | 16988 | D-ribose | + | builds acid from |
43273 | 65327 | D-xylose | + | builds acid from |
43273 | 15963 | ribitol | + | builds acid from |
43273 | 12936 | D-galactose | + | builds acid from |
43273 | 17634 | D-glucose | + | builds acid from |
43273 | 15824 | D-fructose | + | builds acid from |
43273 | 16024 | D-mannose | + | builds acid from |
43273 | 62345 | L-rhamnose | + | builds acid from |
43273 | 17268 | myo-inositol | + | builds acid from |
43273 | 16899 | D-mannitol | + | builds acid from |
43273 | 17924 | D-sorbitol | + | builds acid from |
43273 | 17057 | cellobiose | + | builds acid from |
43273 | 28053 | melibiose | + | builds acid from |
43273 | 17992 | sucrose | + | builds acid from |
43273 | 27082 | trehalose | + | builds acid from |
43273 | 17151 | xylitol | + | builds acid from |
43273 | 28066 | gentiobiose | + | builds acid from |
43273 | 62318 | D-lyxose | + | builds acid from |
43273 | 28847 | D-fucose | + | builds acid from |
43273 | 17108 | D-arabinose | + | builds acid from |
43273 | 18403 | L-arabitol | + | builds acid from |
43273 | 5291 | gelatin | + | hydrolysis |
43273 | 28017 | starch | - | hydrolysis |
43273 | 17632 | nitrate | - | reduction |
43273 | 16301 | nitrite | - | reduction |
43273 | skimmed milk | + | degradation | |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43273 | 8653 | pyocyanine | no |
43273 | 16136 | hydrogen sulfide | no |
43273 | 35581 | indole | no |
43273 | 15688 | acetoin | no |
68369 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
43273 | 15688 | acetoin | - | |
68369 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43273 | beta-galactosidase | - | 3.2.1.23 |
43273 | lysine decarboxylase | - | 4.1.1.18 |
43273 | ornithine decarboxylase | - | 4.1.1.17 |
43273 | urease | - | 3.5.1.5 |
43273 | tryptophan deaminase | - | 4.1.99.1 |
43273 | arginine dihydrolase | + | 3.5.3.6 |
43273 | catalase | + | 1.11.1.6 |
43273 | cytochrome oxidase | + | 1.9.3.1 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | + | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage 43273 C12:0 2.6 43273 C16:0 31.7 43273 C18:0 1.1 43273 C18:1ω7c 17 43273 C10:0 3OH 2.8 43273 C12:0 2OH 4.1 43273 C12:0 3OH 3.9 43273 cyclo-C17:0 5.9 43273 C16:1ω7c / C15:0 iso 2OH 29.5 - type of FA analysis: whole cell analysis
- incubation medium: TSB
- incubation temperature: 28
- incubation time: 1
- system: MIS MIDI
- cutoff value:
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
42906 | - | - | - | + | - | - | + | - | + | + | + | + | + | - | + | + | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
42906 | bovine raw milk | Southern Gemany | Germany | DEU | Europe |
43273 | raw bovine milk |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Bovinae (Cow, Cattle) |
#Host Body Product | #Fluids | #Milk |
taxonmaps
- @ref: 69479
- File name: preview.99_199.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_199&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: KP756923
- Sequence Identity:
- Total samples: 8172
- soil counts: 874
- aquatic counts: 1631
- animal counts: 3216
- plant counts: 2451
Safety information
risk assessment
- @ref: 42906
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 42906
- description: Pseudomonas lactis strain DSM 29167 16S ribosomal RNA gene, partial sequence
- accession: KP756923
- length: 1428
- database: ena
- NCBI tax ID: 1615674
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudomonas lactis DSM 29167 | GCA_001439845 | contig | ncbi | 1615674 |
66792 | Pseudomonas sp. DSM 29167 | 1615674.3 | wgs | patric | 1615674 |
66792 | Pseudomonas lactis DSM 29167 | 2660238313 | draft | img | 1615674 |
GC content
@ref | GC-content | method |
---|---|---|
43273 | 60 | |
42906 | 60.0 | sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 91.272 | yes |
gram-positive | no | 98.116 | yes |
anaerobic | no | 98.294 | no |
halophile | no | 92.49 | no |
spore-forming | no | 94.397 | yes |
thermophile | no | 99.902 | yes |
glucose-util | yes | 93.064 | yes |
flagellated | yes | 81.681 | no |
aerobic | yes | 94.563 | yes |
glucose-ferment | no | 91.18 | yes |
External links
@ref: 42906
culture collection no.: DSM 29167, LMG 28435
straininfo link
- @ref: 96604
- straininfo: 403013
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 28141500 | Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk. | von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001836 | 2017 | Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Milk/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Phylogeny | 31922466 | Pseudomonas carnis sp. nov., isolated from meat. | Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Bantleon A, Goesmann A, Kalinowski J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003928 | 2020 | Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Genes, Bacterial, Germany, Meat/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Poultry, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine, Ubiquinone/chemistry | Biotechnology |
Phylogeny | 34309505 | Pseudomonas lactucae sp. nov., a pathogen causing bacterial rot of lettuce in Japan. | Sawada H, Fujikawa T, Satou M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004917 | 2021 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Japan, Lettuce/*microbiology, Nucleic Acid Hybridization, *Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
42906 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29167 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29167) | |||
43273 | Mario von Neubeck, Christopher Huptas, Claudia Glück, Manuel Krewinkel, Marina Stoeckel, Timo Stressler, Lutz Fischer, Jörg Hinrichs, Siegfried Scherer and Mareike Wenning | Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp.nov., isolated from bovine raw milk | 10.1099/ijsem.0.001836 | IJSEM 67: 1656-1664 2017 | 28141500 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
68369 | Automatically annotated from API 20NE | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
96604 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403013.1 |