Name: Vulcanibacillus modesticaldus BR
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1402

Type strain:

Species: Vulcanibacillus modesticaldus

Strain Designation: BR

Culture col. no.: DSM 14931, JCM 12998

Strain history: DSM 14931 <-- M. Miroshnichenko strain BR.

NCBI tax ID(s): 337097 (species)

SI-ID 291020

DSM 14931, JCM 12998

Section

Vulcanibacillus modesticaldus BR is an anaerobe, chemoorganoheterotroph, spore-forming bacterium that forms punctiform colonies and was isolated from core sample collected at hydrothermal vent field.

colony-forming
Gram-positive
rod-shaped
spore-forming
anaerobe
chemoorganoheterotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Vulcanibacillus*
	Species	**Vulcanibacillus modesticaldus**
	Full Scientific Name (LPSN)	Vulcanibacillus modesticaldus L'Haridon et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #23027]	Gram stain	positive

[Ref.: #23027]	Cell length	4.0-5.0 µm

[Ref.: #23027]	Cell width	0.2-0.3 µm

[Ref.: #23027]	Cell shape	rod-shaped

[Ref.: #23027]	Motility	no

[Ref.: #23027]	Colony size	2.0 mm
[Ref.: #23027]	Colony color	cream
[Ref.: #23027]	Colony shape	punctiform
[Ref.: #23027]	Incubation period	5 days
[Ref.: #23027]	Cultivation medium used	EM agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5451]

Culture medium

VULCANIBACILLUS MEDIUM (DSMZ Medium 1042)

[Ref.: #5451]

Culture medium growth

[Ref.: #5451]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5451]

Culture medium composition

expand / minimize

[Ref.: #23027]

Culture medium

EM agar

[Ref.: #23027]

Culture medium growth

		Temperature
[Ref.: #5451]	growth	45 °C
[Ref.: #23027]	growth	37.0-60.0 °C
[Ref.: #23027]	optimum	55.0 °C
[Ref.: #67770]	growth	42 °C

[Ref.: #5451]	Temperature range	thermophilic
[Ref.: #23027]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #23027]			optimum	7
[Ref.: #23027]			growth	6.0-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5451]	Oxygen tolerance	anaerobe
[Ref.: #23027]	Oxygen tolerance	obligate anaerobe

[Ref.: #23027]

Nutrition type

chemoorganoheterotroph

[Ref.: #23027]	Type of spore	endospore
[Ref.: #23027]	Ability of spore formation	yes

	Salt		Salt conc.
[Ref.: #23027]	NaCl	growth	10.0-40.0	g/L
[Ref.: #23027]	NaCl	growth	20.0-30.0	g/L
[Ref.: #23027]	NaCl	optimum	2.0-3.0	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23027]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #23027]	fe(iii) citrate	-	electron acceptor
[Ref.: #23027]	ferric oxide ChEBI	-	electron acceptor
[Ref.: #23027]	nitrate ChEBI	+	electron acceptor
[Ref.: #23027]	nitrate ChEBI	+	reduction
[Ref.: #23027]	nitrite ChEBI	-	electron acceptor
[Ref.: #23027]	sulfate ChEBI	-	electron acceptor
[Ref.: #23027]	thiosulfate ChEBI	-	electron acceptor

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #23027]		catalase	-	1.11.1.6
[Ref.: #23027]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5451]	Sample type/isolated from	core sample collected at hydrothermal vent field
[Ref.: #5451]	Geographic location (country and/or sea, region)	Mid-Atlantic Ridge, Rainbow vent field
[Ref.: #5451]	Geographic location	36.2333°/-33.9000°

[Ref.: #23027]	Sample type/isolated from	deep-sea hydrothermal core sample at the Rainbow vent field
[Ref.: #23027]	Enrichment culture	enrichment medium (EM)
[Ref.: #23027]	Enrichment culture composition	NH4Cl, 0.33; KCl, 0.33; KH2PO4 , 0.33; CaCl2.2H2O, 0.33; MgCl2.6H2O, 0.33; NaCl, 25.0; NaNO3 , 3.0, sodium acetate, 2.0; yeast extract, 0.1; trace element solution, 10 ml l-1 vitamin solution, 10 ml l-1

[Ref.: #67770]	Sample type/isolated from	Deep-sea hydrothermal core sample collected at the Rainbow vent field on the Mid-Atlantic Ridge
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Terrestrial	#Core sample
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM050346 (>99% sequence identity) for Vulcanibacillus modesticaldus subclade from Microbeatlas

Aquatic Samples	4
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	5

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5451]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5451]	Vulcanibacillus modesticaldus 16S rRNA gene, type strain BRT	AM050346	1526	ENA	337097 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Vulcanibacillus modesticaldus BR	GCA_001730235	contig	GenBank	337097 tax ID

[Ref.: #23027]

GC-content

34.5 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Vulcanibacillus modesticaldus BR NCBI: GCA_001730235.1
[Ref.: #69480]	flagellated	no	85.449	yes
[Ref.: #69480]	gram-positive	yes	51.577	no
[Ref.: #69480]	anaerobic	yes	81.69	no
[Ref.: #69480]	aerobic	no	94.171	yes
[Ref.: #69480]	halophile	no	69.547	no
[Ref.: #69480]	spore-forming	yes	95.508	yes
[Ref.: #69480]	thermophile	yes	99.48	yes
[Ref.: #69480]	glucose-util	yes	81.718	no
[Ref.: #69480]	motile	yes	82.081	yes
[Ref.: #69480]	glucose-ferment	no	70.249	no

Availability in culture collections External links

[Ref.: #5451]

Culture collection no.

DSM 14931, JCM 12998

[Ref.: #71053]

SI-ID 291020

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, nitrate-reducing bacterium from deep-sea hydrothermal vents.	L'Haridon S, Miroshnichenko ML, Kostrikina NA, Tindall BJ, Spring S, Schumann P, Stackebrandt E, Bonch-Osmolovskaya EA, Jeanthon C	Int J Syst Evol Microbiol	10.1099/ijs.0.64012-0	2006	*

References

#5451

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14931

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23027

S. L'Haridon,M. L. Miroshnichenko,N. A. Kostrikina,B. J. Tindall,S. Spring,P. Schumann,E. Stackebrandt,E. A. Bonch-Osmolovskaya,C. Jeanthon: Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, nitrate-reducing bacterium from deep-sea hydrothermal vents. IJSEM 56: 1047 - 1053 2006 ( DOI 10.1099/ijs.0.64012-0 , PubMed 16627653 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71053

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID291020.1 )

* These data were automatically processed and therefore are not curated

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